Fantastic Properties

of Nano-bubbles

Kaneo Chiba
Masayoshi Takahashi

Burstatthesurface

Goesup
rapidly

Disappear
Continue
Shrinking

Ordinarybubble
(Macrobubble)

Microbubble

Nanobubble

Nano-bubble production by collapsing microbubble

Waste water treatment
OH
Microbubble
Decomposition
of organic chemicals

Collapse
OH

Radical generation

Physical stimulus

Nanobubble production

with salt (electrolytes)

Microbubble

Collapse

Nano-bubble
Physical stimulus

Nono-bubble generator

Microbubble
Nano-bubble

Physical stimulus

Analysis of nano-bubble by Dynamic light scattering (DLS)

20
18
16
14
12
10
8
6
4
2
0
76

93

113

138

168

204

249

304

370

Particle size (nm)

The deference of size between MB and NB

Microbubble 10 30m

Nano-bubble
< 200nm

The deference of life time between MB and NB

Disappear
Continue
Shrinking
Microbubble

Several minutes

Nanobubble

Several months
Why ?

Electrode
(+)

Light

Electrode
( +)

Water + microbubble
Electrophoresis cell
Microscope

Movement of microbubbles in electrophoresis cell

Change in potential of microbubble over time

Ions

time
Ions

Microbubble

Accumulation of surrounding ions

potential mV

-80

with salt (electrolytes)

-70
-60

time

-50
-40
-30
-20
0

10

15

20

Diameter m

25

30

Stabilization mechanism of nanobubble (assumption)

Nano-bubble

Negatively charged

Aqueous solution

Surrounded by accumulated ions

(counter ions)
Salting out
Prevention of gas dispersion

Nano-bubble generator

Normal ozone water vs. Ozone nano-bubble

Ozone water ; disinfection & sterilizing Food industry, medical
Normal method

1.5mg/L

Ozone

0.1mg/L
1 hour

Bubbling
Nano-bubble

Collapse

1.5mg/L
Micro-bubble

Nano-bubble

More than
1.0mg/L
100 days

13

How to treat the problem of norovirus

During
cultivation

Norovirus

Norovirus symptoms:
vomiting, diarrhea,
a low-grade fever, chills,
headache, muscle aches

Oyster
14

Conventional method

Oyster
Chlorine

Norovirus

Oyster

Norovirus

Chlorine ( high concentration )

----- not good for foods

Ozone water
Ozone = strong oxidation ability

Virus inactivation

15

Oysters in ozone nanobuble water for 1 day

16

Before ozone nanobubble treatment

17

After ozone nanobubble treatment

More than 99% of feline calicivirus in the body --- inactivated

18

Practical method for inactivation of norovirus by ozone nano-bubble

19

Active effect for fishes

Carps : recovering from Carp herpes ( serious disease )

20

Oxidation ability of ozone nano-bubble as a form of mist

21

more than 80% of original oxidation ability

reduction of smell and bacteria from atmospheres
22

Oxygen nanobubble

Collapse of oxygen microbubble

About 1% salt
Recovery of weakened fishes

Active effect for creatures

23

Nano-Bubble Aquarium

24

Astonishing effects on plant

Orchid in oxygen NB water

for more than 1 month

Still alive

25

Initial growth of plants

White radish
Broccoli

Tap water

White radish
Broccoli

Oxygen nano-bubble water

26

Active effect for oysters

Room temperature

-20 for 1 day in oxygen NB

Still alive

After 1 hour
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Application of nanobubble in medicine

Prevention of arteriosclerosis (hardening of blood vessel )
Oxygen nanobubble inhibits mRNA expression induced by
TNF in RAOECs
ICAM1

VCAM1
Heart attack

GAPDH

Stroke
control

Culture aorta endothelium of rat

Cytokine stimulationTNF alpha

TNF
alpha

control

TNF
alpha

With oxygen
nanobubble

Department of Cardiology,
Jichi Medical University

28

Application of nanobubble in medicine

Preservation of living tissue / organ
Vagus nerve of rat : after 1 week
Isotonic NaCl solution

Oxygen nanobubble

Tokyo Medical and Dental University

29

Thank you very much

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