Gargi Singh, PHD: Research Talk at Iit Roorkee, 2016

Gargi Singh, PhD
Research talk at IIT Roorkee, 2016
PhD Civil Engineering, Sustainable Nanotechnology; Virginia Tech

Biodegradation of Nanocellulose and Microbial Community Response:
Effect of Surface Modification and Morphology 2015
MS Civil Engineering; Virginia Tech

Influence of Petroleum Deposit Geometry on Local Gradient of Electron Acceptors and
Microbial Catabolic Potential 2012
BTech Civil Engineering; IIT Roorkee 2009

Sustainable Nanotech Future Research Teaching
Unintended Consequences 1
Hero or Villain?
Tetra Ethyl Lead (in
Gasoline)
Freon (a CFC)
Sustainable Approach
Environmental Fate
Thomas Midgley Impact on Relevant
Environments: Global
Nutrient Cycles
Environmental Sustainability 2
Nanotechnology
APPLICATION IMPLICATION
Matthew Chan Gargi Singh

Application Implication
Environmental Sustainability 2
Nanotechnology Green ideal material!!

Nanocellulose Distinctions
Derived from cellulose Nano-size range

Wide range of properties Wide range of surface
& applications modifications
High strength Different morphologies
Low density Has nanocellulosic core,
Expected market of 1 but questionable
billion USD by 2020 environmentally
friendliness
Nanocellulose Fate and Environmental Impact 3
Differences in size, surface charge & morphology affects

properties of nanocellulose
Preparation procedure
Source
Cellulose degrading enzymes specific to size, surface charge
and morphology
Impact cellulose degradation & C-cycle in receiving

environments
Biodegradability & environmental impact??

Relevant Environments
Partitioning into
Community sludge
Community Waste
waste
Anaerobic digesters
Relevant Environments
Community waste Dumpsites
Wetlands
- Global Carbon-cycling
- Receiving sinks
- Impacted and impacting
- Community resource: fish & water
Nanocellulose Biodegradation 6
Singh, G., Pati, P., Chandoha-Lee, C., Zhang, W., Vikesland P.J., Renneckar, S., Pruden, A. 2016. Biodegradation of
Nanocrystalline Cellulose by Environmentally-relevant Consortia. (Under review for Water Research)
Singh, G., Pati, P., Chandoha-Lee, C., Zhang, W., Vikesland P.J., Renneckar, S., Pruden, A.. Effect of Nanocellulose Surface
Charge and Morphology on Biodegradation and Microbial Community Shifts of Two Environmentally-relevant Microbial Communities.
(Under Review for Environmental Science & Technology)
Biodegradation rates
Size:
Nano
Micro
Surface Chemistry:
Negatively
charged
Positively
charged
Morphology:
Crystalline rod
Fibrillated
Levels of Gene Markers 7
cel48 16S
rRNA
cel48 16S
rRNA
Metagenomic Investigation of Nanocellulose Biodegradation 8
Next generation sequencing

A new and powerful technique
- Characterization on both taxonomic and functional gene level
- Advanced fundamental understanding of cellulose degradation
- Insight into potential for toxicity
Development of a novel algorithm: MetaStorm
- Robust assembly to scaffold level.

- Higher diversity of cellulose degrading enzymes than any other
metagenomic or otherwise study.
Argoty, G.A., Singh, G., Heath, L., Pruden, A., Zhang, Q.
MetaStorm: A Public Resource for Customizable Metagenomics Annotation. (Under review for Nucleic Acids Research)
Evolution of Molecular Biology Tools 10
2011 2012 2013-14 2014-15
qPCR qPCR, rep-PCR qPCR qPCR
DGGE t-RFLP Pyrosequencing based Illumina based
amplicon sequencing amplicon sequencing
Cloning followed by Culturing followed by - Whole sample

Sanger sequencing Sanger sequencing metagenomics
DGGE based bacterial community profiling
Singh, G., Widdowson, M., and Pruden. A. 2012. Influence of Petroleum Deposit Geometry on Local Gradient of Electron Acceptors
and Microbial Catabolic Potential. Environmental Science and Technology. 46 (11): 57825788.
2011 2012 2013-14 2014-15

rep-PCR Pyrosequencing based bacterial community profiling

Hoyland, V.W., Knocke W.R., Falkinham III J.O., Pruden A., Singh G. 2014. Effect of Drinking Water Treatment Process Parameters on Biological
Removal of Manganese from Surface Water. Water Research. 66: 31-39.
2011 2012 2013-14 2014-15

Pyrosequencing
qPCR based
based community
evidenceprofiling
for
sulfate reduction
Xu, J., Veeramani, H., Qafoku, N., Singh, G., Breazeal, V., Kukkadapu, R., Pruden, A., Hochella, M. F. Jr. Efficacy of Biostimulation for Uranium
Sequestration: Coupled Effects Sediment/Groundwater Geochemistry and Microbiology (Submitted to Microbial Ecology)
2011 2012 2013-14 2014-15

Illumina based
community profiling
Who?
Who?
Enzymatic Families
10
Bacterial Classes
Who is doing What?

An Interdisciplinary Approach 11
Biodegradability & Environmental Impact of Nanocellulose
Nanocellulose with contrasting
Sustainable surface charges & Environmental
Biomaterials morphologies Nanotechnology
AFM
Raman
Nanocellulose characterization DLS
Environmental Zeta Potential
Microbiology X-ray powder
Biodegradation diffraction
Consortia from Biodegradation Assays
Anaerobic Digester
& Wetland
Molecular
Taxonomic profiles & biology
Gene Biomarkers qPCR
Next Generation
Computing & DNA sequencing
Bioinformatics Metagenomics
MetaStorm
Degradation: Who? How?
Future Work 13
Open defecation fields as a major sink and source of antibiotic
resistance potential
Teaching and Service 15
Training in Pedagogy
GRAD 5104 Preparing Future Professoriate
GRAD 5114 Contemporary Pedagogy
Charles E. Via, Jr. Academic Prep Teaching

Assistantship
- Global Climate Change Game CEE 3104: Introduction to
- Press Conference for Washington DC Lead Case Environmental Engineering,
- Continual Feedback Platform Virginia Tech
- Online Discussion Forum
Acknowledgements

Gargi Singh, PHD: Research Talk at Iit Roorkee, 2016

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Gargi Singh, PhD

Research talk at IIT Roorkee, 2016

PhD Civil Engineering, Sustainable Nanotechnology; Virginia Tech

MS Civil Engineering; Virginia Tech

BTech Civil Engineering; IIT Roorkee 2009

Matthew Chan Gargi Singh

Nanotechnology Green ideal material!!

Derived from cellulose Nano-size range

Differences in size, surface charge & morphology affects

Impact cellulose degradation & C-cycle in receiving

Biodegradability & environmental impact??

Community waste Dumpsites

Next generation sequencing

- Robust assembly to scaffold level.

Cloning followed by Culturing followed by - Whole sample

DGGE based bacterial community profiling

Cloning followed by Culturing followed by - Whole sample

rep-PCR Pyrosequencing based bacterial community profiling

Cloning followed by Culturing followed by - Whole sample

Cloning followed by Culturing followed by - Whole sample

Who is doing What?

Charles E. Via, Jr. Academic Prep Teaching

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