PROFILED COMPANY
BioLargo, Inc.
BioLargos Advanced Oxidation
System (AOS) Poised to Impact
$862 Billion Global Water Market
OTCQB: BLGO
G lobal Water Intelligence, a leading industry market
researcher, estimates the annual global water market
to be $862 billion.
Goldman Sachs calls water the petroleum
for the next century1 adding, investors who
know how to play the infrastructure boom
will reap huge rewards. With global water
consumption doubling every 20 years, they
predict that by 2025 about one third of the
global population will not have access to
adequate drinking water.
EXISTING WATER TREATMENT
TECHNOLOGIES ARE
EXPENSIVE AND OFTEN
INADEQUATE
After examining data from more than 36,000
water samples collected nationwide by the
U.S. Environmental Protection Agency from
2013 to 2015, Harvard researchers found
unsafe levels of toxic chemicals in the drink-
ing water of 33 states2. In 2016, a USA Today
Investigation reported that after analyzing
samples from 2012 through 2015, unsafe
1 http://www.telegraph.co.uk/finance/
newsbysector/utilities/2791116/Water-crisis-to-
be-biggest-world-risk.html
2 http://news.harvard.edu/gazette/
Dennis Calvert, CEO story/2016/08/unsafe-levels-of-toxic-chemicals-
found-in-drinking-water-of-33-states/
28 MicroCap Review Magazine
drinking water was found with excessive lead
levels in All 50 States3.
While some of the violations involve
pathogens such as Legionella, most violations
appear to involve recalcitrant contaminants
such as lead and mercury that are difficult to
remove with existing water treatment tech-
nologies. The EPA lists4 over 80 potentially
harmful contaminants found in their Table
of Regulated Drinking Water Contaminants,
most of which are difficult for existing water
treatment plants to remove. Some of the bet-
ter-known contaminants are: lead, arsenic,
mercury, nitrate, benzene, and pharmaceuti-
cal residues.
For the most part, modern water treat-
ment plants perform reasonably well for
disinfection, albeit with high capital and
operational costs. However, based on the
numerous water safety violations, water
treatment plants can fall short when it comes
to removing recalcitrant contaminants.
3 https://weather.com/health/news/lead-
contamination-water-usa-today-study
4 https://www.epa.gov/ground-water-and-
drinking-water/table-regulated-drinking-water-
contaminants
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Figure 1
TECHNOLOGY GAP WITH
CURRENT WATER TREATMENT
TECHNOLOGIES
Today, chlorine remains one of the most
commonly used water treatment technolo-
gies for standard disinfection processes, but
is under increasing scrutiny due to envi-
ronmental concerns regarding its potential
byproducts. UV (ultraviolet light) is gaining
popularity because it does not leave any toxic
residue or byproduct, however, it is costly to
purchase and operate, requiring substantial
input energy. Ozone is another technology
that can be effective, but is very expensive
to purchase and operate, and requires high
levels of input energy.
Filtration and membrane technologies are
also commonly used to filter out unwanted
contaminants, but they are fraught with
high maintenance costs and biofilm fouling.
Some, such as reverse osmosis systems, often
require significant energy and can have lim-
ited efficiency.
The world needs more effective clean
water technologies that are inexpensive to
build and inexpensive to operate in order to
meet the ever-increasing demand for clean,
safe water.
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BIOLARGOS AOS:
BREAKTHROUGH LOWEST
COST - HIGHEST IMPACT CLEAN
WATER TECHNOLOGY
Figure 1 BioLargo AOS Alpha Unit Built by
Northern Alberta Institute of Technology
After millions of dollars in R&D and more
than 30 government grants (and counting),
BioLargos research and development team
recently showcased the first pre-commercial
prototype of its patented AOS water treat-
ment system, billed as the lowest cost and
highest impact, scalable clean water technol-
ogy in the world.
Test results show that the AOS achieves
unprecedented rates of disinfection, and
eliminates infectious biological pathogens
such as Salmonella enterica, Listeria mono-
cytogenes and E. coli. The AOS has been
shown to be 100x more effective at eliminat-
ing Salmonella than the best-in-class poul-
try production water disinfectant, chlorine
dioxide. The AOS has also been proven
effective in the elimination of toxic oil & gas
water contaminants such as naphthenic acids
and polyaromatic hydrocarbons. Research
suggests the AOS also has the potential to
remove soluble chemical contaminants such
as acids, solvents, sulfurs, pharmaceutical
by-products, nitrates, phosphates, mercury,
copper, lead, aluminum, and most contami-
nants identified by the EPA. Lab-scale test-
ing is planned to verify efficient removal
of these contaminants. Past results confirm
that the AOS can match the best-in-class
technologies in performance at destroying
soluble organics while using less than 1/20th
the energy and operating more than 10X
Test results show that the AOS achieves
unprecedented rates of disinfection, and eliminates
infectious biological pathogens such as Salmonella
enterica, Listeria monocytogenes and E. coli.
faster than the commonly accepted industry
protocol, hydrogen peroxide and ozone.
The AOS is scalable and modular in
design. The AOS is also unique in its ability
to deliver residual iodine where the custom-
ers desire, or it can remove residual iodine as
needed. Its flexibility allows it to be built to
meet a wide variety of capacity and flow-rate
requirements, and the company is already
working on what it calls Gen 2 for ultra-
high flow rates. The AOS Beta, which will
feature full data-logging and automation
features, is planned for early 2017.
BIOLARGO AND INDUSTRY
GIANT CHICAGO BRIDGE &
IRON FORM RELATIONSHIP TO
IMPLEMENT AOS AND OTHER
BIOLARGO TECHNOLOGIES
BioLargo recently announced a new rela-
tionship with CB&I to support implemen-
tation of BioLargos advanced water and
air technologies and provide independent
performance verification. News of the CB&I
relationship sends a signal to the investment
world and to big industry that BioLargos
advanced water treatment technology has an
important contribution to help make clean
water affordable in a wide variety of indus-
tries. The relationship also sets the stage
to pursue business opportunities in which
BioLargos technologies or products will be
used to serve clients of CB&I and BioLargo.
With 40,000 employees and a 2016 back-
log of $20 Billion, CB&I is a world-leading
engineering, procurement, fabrication, and
construction company, and a provider of
environmental and infrastructure services.
MicroCap Review Magazine 29

BioLargo recently announced a new relationship
with CB&I to support implementation of
BioLargos advanced water and air technologies and
provide independent performance verification.
CB&I builds oil refineries, liquefied natural
gas terminals, wastewater treatment plants,
offshore platforms, and power plants. CB&I
is the worlds largest storage tank construc-
tion company, with tanks constructed for
the oil & gas, mining, municipal water, and
wastewater industries. They are also actively
involved in the remediation and manage-
ment of hazardous waste.
According to BioLargo CEO Dennis
Calvert, all of BioLargos technologies can
serve a wide array of industrial customers.
He adds, Our mission to make life better
includes helping industry tackle operational
cost challenges effectively. That intersection
of service is likely where our new relationship
with CB&I will shine the brightest and we
look forward to working with the exceptional
team at CB&I to serve industry.
BIOLARGO PRODUCTS FOR
CLEAN AIR AND ADVANCED
WOUND CARE ALSO FINDING
COMMERCIAL PATH
Since the first sale in May of 2016, BioLargo
has focused on marketing its CupriDyne
The opportunities for BioLargo to make a
meaningful contribution for a healthy world
are massive. The company has invested years
developing, refining and validating innovative
products to make life better.
30 MicroCap Review Magazine
Clean industrial odor control prod-
ucts for the large waste-handling markets.
Management reports, We have a number
of trials underway with leading companies
in the waste-handling industry, and the feed-
back from customers confirms our belief that
they want and need a reliable and afford-
able solution to their inherent odor manage-
ment issues. CupriDyne Clean eliminates
powerful odor-causing compounds like H2S
and ammonia instantly on contact, thereby
maintaining clean, safe, and healthy air. The
product is safe and non-toxic for humans,
animals, and plants. As the word gets out,
and after clearing the usual lengthy hurdles
involved with becoming a vendor for large
corporate and government accounts, man-
agement believes that sales will grow with
national supplier contracts.
In January of 2016, BioLargo announced
that its Clyra Medical subsidiary closed
a $5.75 million financing package for its
Advanced Wound Care products. Cash of
$750,000 was earmarked for Clyra to com-
plete its product development work, testing,
labeling and ultimately its FDA application
and approval which, due to the successful
development work over the past year, is now
scheduled 2017. An additional $5 million
line of credit was committed by the inves-
tor to support commercialization once FDA
approval is complete. Clyra is targeting late
2017 early 2018 to be ready for market.
LOOKING AHEAD
The opportunities for BioLargo to make a
meaningful contribution for a healthy world
are massive. The company has invested years
developing, refining and validating innova-
tive products to make life better. As the
Company continues to recruit highly quali-
fied people, engage strategic relationships,
and properly capitalize various business
opportunities, management believes its tech-
nology and products will continue to find a
successful path forward to significant com-
mercial success as well as an important con-
tribution to helping people and the planet. n
The company paid consideration to SNN or its affiliates for this article.
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