Creative Biolabs Released Antibody Affinity Maturation Service

It is learned from the news PR news that with the common effort of its scientists, Creative Biolabs released unique
antibody affinity maturation service for antibody affinity maturation improvement.

Antibodies are used extensively in diagnostics and as therapeutic agents. Achieving high-affinity binding is crucial
for expanding detection limits, extending dissociation half-times, decreasing drug dosages and increasing drug
efficacy. In order to conquer the shortages of common antibodies, Creative Biolabs developed antibody affinity
maturationservice.

Weve gained extensive experience in antibody affinity maturation over the years. We use scFv as the antibody
format and monovalent display phagemid as the system to reduce the avidity effects during antigen-binding
screening, said Dr. Monika, scientific officer of Creative Biolabs.

As Dr. Monika introduced, untargeted mutagenesis and oligonucleotide-directed mutagenesis are utilized to
construct random or defined sub-libraries to introduce a large number of mutants of the original
antibody. Antibody binders of higher affinity are then selected by increasing the screening stringency. By
constructing a series of sub-libraries of a scFv/Fab antibody, its protocol allows increase of the affinity of the scFv
antibodies from 10 -8 to 10-9.

Untargeted Mutagenesis

Creative Biolabs uses an error-prone PCR integrated DNA-shuffling approach to mutate main CDR regions during
sub-library construction. If the potential of introducing immunogenic mutations to framework positions is not a
concern, they usually use this approach to create mutations at completely random positions across the entire VH
and VL fragments.

Oligonucleotide-directed Mutagenesis

If the structure of the antibody/antigen complex is available or modeling the structure of the antibody/antigen is
possible, certain positions can be randomized at a defined diversity to improve the affinity. Creative Biolabs is able
to create any sub-libraries to incorporate the defined mutations utilizing trimer codon technology.

Phage Display Antibody Library Screening

Subsequent library screening will fish out the antibody mutants that have high affinity. Two library screening
strategies are available, "surface-panning" strategy and "solution-sorting" strategy. In the first "surface-panning"
strategy, decreasing concentrations of antigen is surface immobilized. In the second "solution-sorting" strategy, we
have two approaches, selection based on the equilibrium constant (Kd) and selection based on binding kinetics.

In addition to the three introduced above, Creative Biolabs has other approaches used, such as antibody affinity
measurement and peptide affinity maturation.
About Creative Biolabs

Creative Biolabs is specialized in providing custom biotechnology and pharmaceutical services that cover the full
scope of biotechnology needs of early drug discovery and drug development, such as T cell production, antibody
protein sequencing, etc.

Resource:
http://news.usaonline.us/Press-Releases/Creative-Biolabs-Released-Antibody-Affinity-Maturation-Service-96581