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SKELETAL MUSCLE: MYOCYTE
Under TEM: I band is bisected by a dark
transverse line (Z line)
Sarcomere:
Repetitive functional subunit of the contractile
apparatus
Extends from Z line to Z line
SKELETAL MUSCLE: SATELLITE CELLS
Responsible for the skeletal muscles ability to
regenerate
Normally quiescent but becomes activated and re-
enter the cell cycle in response to muscle injuries
Proliferate and give rise to new myoblasts
Interposed between the plasma membrane of
the muscle fiber and its external lamina
Small cells with scant cytoplasm
Single nucleus with a denser and coarser
chromatin than a muscle cell nuclei
SARCOPLASMIC RETICULUM and
TRANSVERSE TUBULE SYSTEM
Sarcoplasmic Reticulum:
Smooth endoplasmic reticulum (SER) in a muscle
fiber
Specialized for Ca2+ ion sequestration
Depolarization T-tubules Release of Ca2+ ions
from SR
CARDIAC MUSCLE
Exclusive to the myocardium
Same types and arrangement of
contractile filaments as skeletal muscle
Exhibit cross-striations and branching
CARDIAC MUSCLE
Spontaneous rhythmic contraction
Involuntary
Autorhythmicity
Centrally located nucleus
With densely staining cross-bands
(intercalated discs)
No regenerative capacity beyond early
childhood
CARDIAC MUSCLE
The intercalated discs represent junctions between
cardiac muscle cells
Fascia adherens
Responsible for ID staining in routine H&E
Holds the cardiac muscle cells at their ends to form the functional
cardiac muscle fiber
Maculae adherens (desmosomes)
Bind the individual muscle cells to one another
Prevent the cells from pulling apart under the strain of regular
repetitive contractions
Gap junctions (communicating junctions)
Provide ionic continuity between adjacent cardiac muscle cells,
thus allowing informational macromolecules to pass from cell to
cell
Permits cardiac muscle fibers to behave as a syncytium while
retaining cellular integrity and individuality
SMOOTH MUSCLE
Occurs as bundles or sheets of elongated
fusiform cells with finely tapered ends
Sidepolar arrangement of myosin heads
(skeletal muscle bipolar)
Dense bodies instead of Z disc
Involuntary control
No striations
SMOOTH MUSCLE
Interconnected by gap junctions
Eosinophilic cytoplasm due to concentrations
of actin and myosin
Centrally located nuclei
Lacks a T-system
Lines walls of viscera
Longitudinal or circular arrangement
Alternate contraction results to peristalsis on the
GI
SMOOTH MUSCLE
Structure of Smooth Muscle
Possess a contractile apparatus of thin (actin) and
thick filaments (myosin II) and a cytoskeleton of
desmin and vimentin intermediate filaments
Actin is the smooth muscle isoform of tropomyosin
No troponin associated with smooth muscle tropomyosin
Essential proteins for smooth muscle contraction
initiation and regulation:
Myosin light chain kinase (MLCK): enzyme that
initiates the contraction cycle after its activation by
Ca2calmodulin complex
Calmodulin: Ca2-binding protein, regulates the
intracellular concentration of Ca2
SMOOTH MUSCLE: RENEWAL and
REPAIR
Respond to injury by undergoing mitosis
Contains regularly replicating populations of
cells:
Smooth muscle in the uterus proliferates during
the normal menstrual cycle and during pregnancy
Smooth muscle of the muscularis externa of the
stomach and colon regularly replicates and may
even slowly thicken during life