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Theros H/I SM and MGI SM adjuvant endocrine therapy in breast cancer. J Clin Oncol.
1999;17(5):1474-81.
Theros H/I SM is a molecular diagnostic test, measuring the
ration of gene expression of genes HOXBI3:IL17BR. It helps 9. Hagemann, IS. Molecular Testing in Breast Cancer.Arch
predicts the clinical outcome for breast cancer patients treated Pathol Lab Med. 2016;140:815-24.
with tamoxifen [21]. Resistance to tamoxifen is been associated
10. National Institutes of Health Consensus Development
with an increased level of gene expression resulting into
Conference statement: adjuvant therapy for breast cancer,
aggressiveness of the tumor [22].
November 1-3, 2000. J Natl Cancer Inst. Monogr 2001;30:5-15.
Theros MGI SM is an additional test that profiles for 5 gene
expressions helping understand the recurrence pattern of the 11. Eifel P, Axelson JA, Costa J, et al. National Institutes of
ER+ breast cancer and reclassify Stage II tumor into Stage I like Health Consensus Development Conference Statement:
or Stage III like outcomes [23]. mRNA samples from formalin adjuvant therapy for breast cancer, November 1-3, 2000. J
fixed paraffin embedded (FFPE) tissues is extracted and q RT- Natl Cancer Inst. 2001;93(13):979-89.
PCR is used to quantify the gene expression. If both tests are 12. http://www.hermarkassay.com/
used together, demonstrates potential advantages over current
diagnostic techniques [24]. 13. Berse B. Julie Lynch for Molecular Diagnostic Testing in
Breast Cancer. Seminars in Onco Nursing. 2015;31(2):108-21.
Next Generation Sequencing of Breast Cancer
14. MammaPrint Test [Internet]. BreastCancer.org. 2014.
Tissues
15. van de Vijver MJ, He YD, van t Veer LJ, et al. A gene-
To identify an active mutation in the tumor, Next Generation
Sequencing (NGS) has made it possible to sequence expression signature as a predictor of survival in breast
hundreds of genes in the tumor cells [25]. It helps sequence cancer. N Engl J Med. 2002;347(25):1999-2009.
multiple genes by gene panel testing and is offered by 16. Knauer M, Mook S, Rutgers EJ, et al. The predictive value
many laboratories. Prime advantage of NGS over single gene of the 70-gene signature for adjuvant chemotherapy in early
testing, includes the potential to sequence large number of breast cancer. Breast Cancer Res Treat. 2010;120(3):655-61.
genes in one single panel. It helps detects all possible genetic
variations and mutations [26]. It is cost effective and requires 17. Albain KS, Barlow WE, Shak S, et al. Prognostic and
less time with compare to single gene testing. Since, NGS predictive value of the 21-gene recurrence score assay in
provide systemic results with all tissue samples irrespective postmenopausal women with node-positive, oestrogen-
of ER/PR status; it helps in personalized therapy for treating receptor-positive breast cancer on chemotherapy: a retro-
breast cancer [24]. spective analysis of a randomised trial. Lancet Oncol.
2010;11(1):55-65.
Conflict of Interest
18. Dowsett M, Cuzick J, Wale C, et al. Prediction of risk of
The author declares no conflict of interest. distant recurrence using the 21-gene recurrence score in
References node-negative and node-positive postmenopausal patients
with breast cancer treated with anastrozole or tamoxifen: a
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*Correspondence to:
Patel Ankur P
Long Island University
C.W.Post college
NY
USA
E-mail: Patelankur39@gmail.com