ANTIDIARRHEAL ACTIVITY TEST OF BAY LEAVES INFUSION

(Syzygium polyanthum [Wight] Walp) TO MALE MICE SWISS STRAIN

INDUCED BY CASTOR OIL

Riska Handiani Jubaedah P17335112217

ABSTRACT

Diarrrheal is still a public health problem in developing countries such as

Indonesia, because it is high morbidity and mortality. Diarrheal may occur due
to increased intestinal motility and absorption disorder compensated by watery
stool. Bay leaves (Syzygium polyanthum [Wight] Walp) has been used by the
community as a seasoning agent. Bay leaves has many benefits in treating
various diseases, one of them as an antidiarrheal potion. This study was aimed
to determine the antidiarrheal activity of bay leaves infusion by observing
defecation frequency, stool weight, stool consistency, as well as the percentage
ratio of marker to the intestine. The examination antidiarrheal activity of bay
leaves infusion conducted with 3 variation concentration which are 12,5% w/v,
25% w/v, and 50% w/v. Antidiarrheal activity testing was done using two
methods: Protection of Diarrheal Method and Transit Intestinal Method. In first
method, we observed defecation frequency, stool weight, and stool consistency.
In second method, we calculated the ratio of intestinal transit, which is the ratio
of a distance traveled by a marker in a certain time compared to total length of
the mice intestine. Antidiarrheal activity test results showed that infusion of bay
leaves (Syzygium polyanthum [Wight] Walp) concentration of 25% w/v and 50%
w/v has an antidiarrheal activity, as it significantly (p < 0,05) decreasing the
frequency of defecation, decreasing weight feses, and increasing stool
consistensy. Antidiarrheal activity were achieved by decreasing the frequency of
diarrheal by the bay leaves infusion concentration of 50% w/v significantly
different (p < 0,05) with control positive. Bay leaves infusion concentration of
50% w/v has similiar activity comparator drug suspension Nodiar 3,69
mg/20gBB insignificantly different (p > 0,05) with a comparator drug
suspension Nodiar 3,69 mg/20gBB. Bay leaves infusion showed no
antidiarrheal effect as an antimotility agent.

Keywords: Bay leaves, antidiarrheal, infusion, Syzygium polyanthum [Wight]

Walp.