European Cells and Materials Vol. 26. Suppl.

3, 2013 (page 57) ISSN 1473-2262

Mesenchymal stem cell effects on microbiological and histopathological

alterations in spondylitis tuberculosis rabbit's healing process
Rahyussalim1, T Kurniawati2, A Syahrurachman3, , N Chairani4
1
Department of Orthopaedic and Traumatology FMUI. 2Stem Cell and Tissue Engineering Cluster
MERC FMUI. 3Department Microbiology Clinic FMUI. 4Department Pathologi Anatomic FMUI.

INTRODUCTION: Mesenchymal Stem Cell (MSC)

has the potency to modulate immune response and to
repair tissues. The existence of MSC in Mycobacterium
tuberculosis microscopical environment is hoped to
reduce its proliferating activity. This research aims to
observe the direct MSC transplantation effect on the Fig. 1: Graph changes in population of Th1 and
vertebral body defect in spondylitis tuberculosis rabbit Th2 from blood samples spondylitis tuberculosis
on microbiological and histopathological aspects of rabbits
bone healing in vivo.
Table 2. Result of healing percentage
METHODS: Fourteen spondylitis tuberculosis rabbits
Modality With MSC Without MSC
were divided into two groups which are the treatment examination n % n %
P

(n=7) and the control group (n=7). The treatment group Microbiological 3 (3/7) 42,9 4 (4/7) 57,1 0,500
underwent total treatment Subroto Sapardan alternative
Histophatological 2 (2/7) 28,6 2 (2/7) 28,6 0,720
6 (TTSSA6) intervention procedure, scaffold and MSC
transplantation and anti tuberculosis drugs
administration, meanwhile control group underwent the
same procedure as treatment group without MSC
transplantation. Th1 and Th2 along with
microbiological and histopathological examination on
the tissue lesion from both group were analysed from
Fig. 2: Image of result of AFB staining, culture,
blood after 6 weeks of incubation. The results were
tested statistically and the healing score was calculated. PCR dan histophatology test from tissue lession
spondylitis tuberculosis rabbits
RESULT: Six weeks after MSC transplantation, Th1
was increased from 4.79% (SD= 2.35) into 30.90% DISCUSSION AND CONCLUSION:
(SD = 30.23) and Th2 was decreased from 42.74% (SD Microbiological and histopathological
= 10.23) into 29.26% (SD = 34.95). Th1/Th2 examination on tissues lesion shows a good
population was increased from 0.12 (SD = 0.08) into healing process from both treatment and control
5.84 (SD = 7.80). Microbiological examination showed group which show that there is no effect of MSC
healing of 3 out of 7 treatment rabbits group (3/7, transplantation to the immune response system on
42.9%) and 4 out of 7 control rabbits group (4/7, the infection of Mycobacterium tuberculosis
57.1%); (p = 0.500). Histopathological examination
which is shown on the alteration of positive into
showed healing of 2 from 7 treatment rabbits and also
2 from 7 control rabbits group (2/7, 28.6%); (p = negative results from 2 out of 7 rabbits on both
0.720). Hence the healing score of both the treatment groups. MSC transplantation does not have
group (n=7; mean score 268.57; SD = 15.74) and the significant effect on ST rabbit's healing process
control group (n=5; mean score 264.00; SD = 16.73) microbiologically and histopathologically shown
were all above 105 (healed) (p = 0.595). by the same total healing score.
REFERENCES: 1 R.J.Basaraba (2008) Experimental
Table 1. Result of Th1 and Th2 examination tuberculosis: the role of comparative pathology in the
Before After
MSC tranplantation MSC tranplantation P discovery of improved tuberculosis treatment
% SD % SD
Th1 4.79 2.35 30.90 30.23 Na
strategies, Tuberculosis 88. 2 K. Dheda, S. Schwander,
Th2 42.74 10.23 29.26 34.95 Na et al (2010) The Immunology of Tuberculosis: From
Ratio of Th1/Th2 0.12 0.08 5.84 7.80 Na
Bench to Bedside, Respirology 15:433-450.3 Shirley H.
J. M, et al (2010) Mesenchymal Stem Cells Reduce
Inflammation while Enchanching Bacterial Clearance
and Improving Survival in Sepsis, Am J Respir Crit
Care Med 182:1047-57. 4 S. Kaufmann (2001) How
Can Immunology Contribute to The Control of
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