GlendaleSchool

#11St.BenedictSt.ParadiseVillage
Project8,QuezonCity

A Research on the Effectiveness

of Oregano as Mouthwash

InPartialfulfillmentofthecourse
RequirementinResearch


Presentedby:

MarjoreyP.Cabugon
MaggiA.Dailisan
JeanPsalmeS.Layno
DexterA.Roque

Presentedto:

Dr.MarilouJuachonPanlilio
Mr.ArnoldAntiporda
Mrs.SherrleneUy
Mr.GeminiMalubay
 February9,2015

ACKNOWLEDGEMENT:
Foremost,wewouldliketoexpressoursinceregratitudetoourEnglishteacher,Ms.

SherrleneUyforthecontinuoussupportofourinvestigatoryproject,forherpatienceand

immenseknowledge.Also,forhelpingusduringprocessesourresearchpaper.

Wewouldalsoliketothankeachandeveryonewhohadhelpedinmakingtheinvestigatory

projectsuccessful.TotheCabugonfamilyforlettingustousetheirequipmentsformakingthe

OreganoMouthwashattheirhouse.Inaddition,Mrs.Laynoforsponsoringthepaymentofthe

laboratorytestsforourproduct.Aboveall,wethankGodforhisguidance,givingusstrength

andknowledgewhenwearemakingthisresearchpaperandallthetime.

ABSTRACT:

This study aims to make an alternative mouthwash using Oregano leaves. The researchers used

Oregano leaves as the main ingredient to produce a healthier mouthwash that is beneficial to the

body too. Oral diseases are qualified as major public health problems in all the regions of the

world. And for all of the diseases, the greatest burden of oral diseases is on disadvantaged and

socially marginalized populations.

Oregano Leaves are proven to cure bacteria that causes bad breath, plaque, and gum diseases, as

well as respiratory infections. Oregano leaves are aromatic, warm, and slightly bitter. Also, this

herb is very affordable and can be made into a mouthwash in just easy steps.

The Oregano Mouthwash is made of the boiled water of 1 cup oregano leaves. The Oregano

leaves are separated from the liquid. Then, the researchers put the liquid into a cup. After 2

weeks, the mouthwash had expired. The researchers arent satisfied of the results and therefore

performed a second trial. For the second trial, the researches put lemon zest to the original

recipe, and it expired after 3 weeks.

The researchers have taken the Oregano mouthwash to a laboratory test that was been done by

chemists in Gensens Incorporated. The researchers got 3 people to be the test subjects. A swab

had been wiped on their inner cheeks before and after they gargled the mouthwash and are given

to the chemists to test it. After 2 weeks, the results have been released. Based on the results, both

the first and second trial mouthwashes are effective.

CHAPTERONE:

BACKROUNDOFTHESTUDY
StatementofObjectives:
Thisstudyaimstomakeanoreganomouthwashwhichwillhelpmanyofustohaveafresher

breathandhealthiermouth.Itischeapersincetheingredientusedcanbefoundinmostofthe

markets.Also,oreganoleavescanalsobeusedtocurecoughs,asthma,coldandflu,croup,

bronchitisandmanymoreillnessesaccordingtowebmd.

Thegoalofthisstudyistomakeanalternativemouthwashthatismadeoforeganoleaves.

Accordingtoanarticlefromdailymail.co.uk,manymouthwashesareproventoraisetheriskof

heartattackandstrokesbecauseofthelargeamountofantisepticchlorhexidinefoundinthe

mouthwashes.Scientistshavesaidthat"chlorhexidinekillsmicrobesneededtohelpcreate

nitrite,essentialforbloodvesselstodilateproperly.Butthemouthwashcausednitriteproduction

inthemouthtofallbyover90percent,andbloodnitritetofallby25percent".Theyalsohave

saidthat"foreachtwopointriseinbloodpressure,theriskofdyingfromheartdiseaserisesby

sevenpercent,accordingtoseparateresearch.Sucharisealsoincreasestheriskofdyingfrom

strokebytenpercent".

Basedontheresearchers'researches,oreganoleavesareantibacterialandantiinflammatory,

whichisgood.Inaccordancetowebmd.com,theleaveswereusedasmedicine.Theresearchers

usedanaturalingredienttoproduceahealthiermouthwashthatalsobenefitsthebodytoo.

Accordingtoanarticlefromabcnews.go.com,withoutproperdentalcare,apersoncanhavea

seriousdentalproblem."Peopledon'trealizethatdentaldiseasecancauseseriousillness,"said

Dr.IrvinSilverstein,adentistattheUniversityofCaliforniaatSanDiego."Theproblemsare

notjustcosmetic.Manypeoplediefromdentaldisease."
Itisplacedindentalhealth.orgthattheneedformouthwashisaresultofaconditioncalled

halitosis,orbadbreath.AndaccordingtoPoulPeterson'sarticle(TheWorldOralHealthReport

2003:continuousimprovementoforalhealthinthe21stcenturytheapproachoftheWHO

GlobalOralHealthProgramme),itisestimatedthatoverhalftheworldpopulationoccasionally

hasfoulsmellingbreathforthereasonofthewidespreaduseoftobaccoandtheincreasing

consumptionofalcohol.Andhealsoaddedthat"oraldiseasesqualifyasmajorpublichealth

problemsowingtotheirhighprevalenceandincidenceinallregionsoftheworld,andasforall

diseases,thegreatestburdenoforaldiseasesisondisadvantagedandsociallymarginalized

populations".

LastApril2011,theKaiserFamilyFoundationreportedthat33percentofpeoplesurveyed

skippeddentalcareordentalcheckupsbecausetheycouldn'taffordthem.Theresearchers

noticedthatmanypeople,nowadays,areignoringtheirhealthconditionsevenifsometimesthey

knowhowseriousitcanbe.Andtherearealsopeoplethataren'tevenawarethattheyalready

havehealthconditions.

Accordingtoanarticle(PsychologicalandSocialImpactsofHalitosis:AReview),halitosishas

becomeamajorhealthconcernamongthegeneralpublicbecauseitcausessignificantamountof

socialdisharmony,embarrassment,frustration,despairandoftenleadstowardsocialand

professionalisolationandmaritalproblems.Badbreathisknowntohaveadevastatingeffecton

thedailysociallifeofthosewhosufferfromitthusearningthetitle'sociallifekiller'.Itis

estimatedtobethethirdmostfrequentreasonforseekingdentalaid,followingtoothdecayand
periodontaldisease.Inwesternsocieties,embarrassmentanddiscomfortarethemainreasonsfor

seekingprofessionalcare.

SignificanceoftheStudy:

Accordingtonatureworldnews.com,thereareclosetofourbillionpeopleintheworldwhosuffer

fromuntreatedoralhealthconditionsthatcausestoothacheandpreventthemfromeatingand

possiblysleepingproperly,whichisadisability.Thistotaldoesnotevenincludesmallcavities

ormildgumdiseases,sowearefacingseriousproblemsinthepopulation'soralhealth.

ThisstudyaimstohelpthegovernmentsuchasDepartmentofHealth(D.O.H.)andPhilHealth.

ItwillbenefitthembecausetheywillnowknowthatOreganoleavescanbeaneffective

mouthwash.Itwillstopcavitiesfromformingwhichpreventsthemfromspreadingtheirvirus

throughcough.Thisstudyalsogoalstohelpstudents,parents,andalsochildrenbecausethe

ingredientstomaketheOreganomouthwashareveryaffordable.Sincetheproductsingredients

arecheap,manypeoplecanmakeitandmorepeoplewillhaveahealthierandcleanermouth.

Themouthwashreducesharmfulsubstancesinthemouththatcausestoothdecayandgum

disease.Whichwillcausetheirmouthtobehealthier.

Thisstudyalsoaimstoprovideourcommunitywitheconomicallybeneficialmouthwash,make

useofreadilyavailableherbalplantsuchasOregano.Themouthwashissafe,ithadnoharsh

chemicalsincluded,andalsoitwilllessenmouthandteethinfections.
Thisstudyalsoaimstoletusknowtheimportanceofusingouravailableresources,howwecan

usethemandthebenefitswecangetfromthem.Also,theresearchersaremakingthisproduct

becausetheywanttoprovethatpeoplecanalsouseherbalmedicinestosolveworldwide

problems.

ScopeandDelimitation:

To be able to make the product, the researchers used dried Oregano leaves, water, pot, sifter,

measuring cups and bottles. Some of the ingredients are needed to be bought in the market while

the materials are readily available in one of the researchers house.

The ingredients (Oregano leaves and water) are affordable for many of the people and can be

made in your houses easily by following the procedure correctly.

The Oregano mouthwash can be made by firstly putting 2 cups of water in a pot and let the water

boil for 15 minutes. After boiling, add 1 cup of oregano leaves and wait until the leaves sank.

Turn off the stove and strain out the Oregano leaves. Then, let the liquid to cool down and put it

in a cup or a bottle.

The general budget that the researchers have to make the product is three hundred (300) pesos.

The researchers only spent for the ingredients was 70 pesos in total. 50 pesos for the Oregano

leaves and 20 pesos for the lemon that is used in the second trial of the mouthwash. The prices

may vary depending on the price that is been sold by the vendors.
The researchers also spent four hundred (400) pesos for the laboratory test. The laboratory test

was made to prove that the product is effective in use. The people that will make the product

doesnt need to test the product in the laboratory too for the researchers already have done it for

them. So, the researchers spent four hundred seventy (470) pesos in total.

The estimated time for the Oregano mouthwash to make is about forty-five (45) minutes in total.

CHAPTERTWO:

REVIEWOFRELATEDLITERATURESANDSTUDIES

HistoryofOregano:

Herb is a plant where their leaves, seeds, or flowers are used for cooking and in

medicine. If you will buy oregano make sure that the kind of oregano you will pick is the

Greek Oregano rather than the common one especially in cooking. Because the

common one does not have any flavour unlike the Greek oregano. Also, herbs can be

used in cosmetics or perfumes. The word herb means green crops. Oregano belongs

to the member of mint family. These things was been based in www.garden.org.

According to www.cals.arizona.edu, herbs that are grown in one specific country,

oregano is a niche crop in Yuma County. This crop becomes the specialty of various

restaurants and it was packed in Yuma and delivered it into US locales. After wards, this

crop becomes popular in culinary treats. Then again, even though oregano was

introduced and known as the specialty of Italian cuisine, still it was originated in Greece.

The word Oregano was came from the Greeks and its meaning joy of the mountain

since oros which means mountains and ganos which means joy or happiness. They

also believed that when their cows grazed the field of oregano, it produces a tastier
meat. Aside from Greece, oregano is also in many cuisines but in different countries like

Mexico, Cuban and Spain.

Also it was said in www.herbsociety.org.uk , that oreganos is the common herb

they used in cooking and as what written in the first paragraph, monks was habitual in

using this herb for food flavouring and medicine. If we go back into the ancient time, as

medieval times they use oreganos as a stewing herb. In addition, the Greeks plant the

oreganos on graves in order to follow their belief that it brought joy to the dead.

Activities:

Herbisaplantwheretheirleaves,seeds,orflowersareusedforcookingandinmedicine.

IfyouwillbuyoreganomakesurethatthekindoforeganoyouwillpickistheGreekOregano

ratherthanthecommononeespeciallyincooking.Becausethecommononedoesnothaveany

flavourunliketheGreekoregano.Also,herbscanbeusedincosmeticsorperfumes.Theword

herbmeansgreencrops.Oreganobelongstothememberofmintfamily.Thesethingswas

beenbasedinwww.garden.org.

Accordingtowww.cals.arizona.edu,herbsthataregrowninonespecificcountry,

oreganoisanichecropinYumaCounty.Thiscropbecomesthespecialtyofvariousrestaurants

anditwaspackedinYumaanddelivereditintoUSlocales.Afterwards,thiscropbecomes

popularinculinarytreats.Thenagain,eventhoughoreganowasintroducedandknownasthe

specialtyofItaliancuisine,stillitwasoriginatedinGreece.ThewordOreganowascamefrom

theGreeksanditsmeaningjoyofthemountainsinceoroswhichmeansmountainsand
ganoswhichmeansjoyorhappiness.Theyalsobelievedthatwhentheircowsgrazedthefield

oforegano,itproducesatastiermeat.AsidefromGreece,oreganoisalsoinmanycuisinesbutin

differentcountrieslikeMexico,CubanandSpain.

Alsoitwassaidinwww.herbsociety.org.uk,thatoreganosisthecommonherbtheyused

incookingandaswhatwritteninthefirstparagraph,monkswashabitualinusingthisherbfor

foodflavouringandmedicine.Ifwegobackintotheancienttime,asmedievaltimestheyuse

oreganosasastewingherb.Inaddition,theGreeksplanttheoreganosongravesinorderto

followtheirbeliefthatitbroughtjoytothedead.

Uses:

According to www.herbsociety.org, Oregano is one of the essential ingredients in Greek, Italian and

French cuisine. Aside from that, the fresh and dried leaves of oregano can be use in soups,

sauces, stew, eggs, chilli, pizza, and more. Also the flowers of oregano can be use as a decoration

for vegetables, salads and other foods because it flowers and leaves has the same flavour. Not

only with cooking, but also in medicines to cure illnesses because oregano can use/ cure colds,

gastrointestinal problems, cough, and a variety of other conditions.

Also it was said by T. Tuttolomondo, S. La Bella, A. Ciacco, and C.Leto that oregano is an aromatic herb which is mostly known for its pleasant. And since

oregano grows wild to the entire island of Sicily, it was decided to use it for cooking in order to satisfy the growing demand for this herb for seasoning.

In addition, oregano can also use to protect generated plants. As hyperhydricity, hyperhydricity is a physiological malformation affecting tissue culture-

generated plants. At which it results in poor regeneration of plants. By using some of nonspecific bacteria, it helps to prevent the hyperhydricity and

improved the acclimation of oregano clones. These information come from these people; K ueno and K Shetty.

Types of Oregano:

There are three common types of oregano. One is heracleoticum or the Greek Oregano. Based on

Project A of horizon herbs, oregano is a prized medicinal and it is used in cooking in which

makes the food spicy because of its aromatic herb. They said that the oregano is very helpful

because it can help in rendering various dishes succulent.

Origanum Majorana is the second type of oregano. It was told in www.ageless.co.za that this

type of oregano was also useful and effective remedy for various ailments. This herb can cure

natural disinfection, antifungal, antibacterial, bruising, tonsillitis, painful menstruation, and

more. Its leaves and flowers also used for creating brews.
The third type of oregano is Origanum Vulgare. According to Dr. Ingrams book, origanum

vulgare was been tested against aggressive germs such as cold, flu and bird flu viruses, all of it

was killed because of it. With this type of oregano, it can also completely destroy human corona

virus. Aside from that, Christopher brown state that the oregano was able to eliminate a host of

bacteria and viruses. In addition, the oregano was considered as the best traditional antibiotic

medications.

Components:

The most important components of oregano are the limonene, gamma-cariofilene, rho-cymene,

canform linalool, alpha-pinene, carvacrol and thymol. The composition of oregano depends on

the type of species, climate, altitude, time or recollection and the stage of growth. Also, all the

characteristics of oregano may enhance the safety and stability of foods. All of these data was

been gathered in www.ncbi.nlm.nih.gov.

It also said in www.ncbi.nlm.nih.gov with different author that the major components of an

oregano essential oil were carvacrol, alcohol, thymol, and terpinene. All extracts were ineffective

in inhibiting the growth of the seven tested bacteria but in hot water extract had the strongest

antioxidant properties and the highest Phenolic content.

According to the people in Ronde Tocht 48 in Zaandam, oregano oil was the most used essential

oil because of its strong antibacterial properties. The components of oregano oil are
phenolicterpenoids, carvacrol, thymol and p-cymene. These components are consisting of great

amount of oregano oil.

Side Effects:

According to www.bio-alternatives.net, using too much oil of the oregano is bad in people who

are allergic to oregano. It can form rash, swelling, and difficulty of breathing. They said that

digestive concerns may also arise because of improper formulation of the oregano. And of

course, the supplements may also interfere with the absorption of iron inside the body. Oregano

oil has only few side effects so most of the time, people who use it can experience the benefits of

its use.

Based on J. Raul Riveras review, oregano has many great advantages but there are some

potential side effects to be aware of. It can lower the amount consumed iron in a body. They also

said that anyone who goes through pregnancy should avoid using oregano oil because it can

energize the blood circulation within the womb which could weaken the lining that encompasses

the foetus. Also, people who had allergic reaction to thyme, basil, mint, or sage must avoid using

oregano.
Review of Related Literatures and Studies:

According to A Critical Review Of The Literature On Mouthwashes and The history of

oral hygiene products: how far have we come in 6000 years? articles, the first reference to

mouth rinsing as a formal practice is credited to Chinese medicine, about 2700 B.C.E., for

treatment of diseases of the gums. The recommendations was rinsing with the urine of a child.

However, in accordance with the Herbal Mouthwashes A Gift Of Nature article,

Pedanius Dioscorides, a Greek physician and surgeon (4090 AD) whose writings served as a

medical textbook, suggested for treatment of bad breath a mouthwash mixture of the following: a

decoct of the leaves of the olive tree, milk, the juice of pickled olives, gum myrrh with wine and

oil, pomegranate peelings, nutgalls, and vinegar. The ancient Romans included teeth cleaning as

part of their religious ceremonies.

 In accordance with Chlorhexidine mouthwash: a review of its pharmacological activity,

clinical effects, uses and abuses., Additional properties and uses of essential oils, For cleaning

oral cavity, containing effervescing agents, volatile oil in spray-dried form and surfactants;

increased foaming, taste, mouth rinses were used for cleaning and disinfecting an individuals

mouth, teeth, gums and breath.