(ENVIRONMENTAL MICROBIOLOGY)

BNEH2123

___E__1. Organisms that live on dead or decaying

organic matter.

__B___ 2. Microorganisms that do not

TUTORIAL 1 cause disease.

_C____ 3. Microorganisms that usually do

not cause disease, but can cause disease
under certain circumstances.
A. pathogens
__D___ 4. The microorganisms that live on
B. nonpathogens us and in us.

C. opportunistic pathogens __A___ 5. The most common causes of

infectious diseases or microbial
D. indigenous microflora
intoxications.
E. saprophytes
F.. 6. Inhabit intestines of animals
F. enteric bacteria
G. 7. caused primarily by serotypes
G. Salmonellosis Typhimurium and Enteriditis

H. typhoid fever H . 8. Serotype typhi

I. enterotoxigenic E. coli I..9. travellers diarrhea

J. Shigellosis J 10. watery or bloody diarrhea, cramps,

fever, malaise
K. Astrovirus
K..11. most common cause of GE in the immuno-
L. Escherichia coli
compromised
M. Salmonella
L..12. Commensal enteric bacterium, but some
N. Lipids strains are pathogenic

O. Nitrobacter M13. Found in particularly high numbers in the

intestines of birds and reptiles

N.14. Complex polymers

O15. Nitrite oxidizing bacteria (NOB)

TUTORIAL 2

_F___ 1. All infectious diseases are caused by pathogens.

__T___2. Pathogens greatly outnumber nonpathogens.

__T___3. Using microorganisms to clean up the environment is known as

bioremediation.

__F___ 4. Microorganisms are essential in the field of genetic engineering.

__T___5. Infectious diseases that are transmitted from animals to humans are known
as zoonoses.

__F___ 6. Vibrio cholera is a Gram (-) oxidase (+) fermentative facultative aerobes

__F___ 7. Vibrio cholera is a Gram (-) oxidase (+) fermentative facultative aerobes

__T___8. Giardia lamblia ia a food borne protozoan parasite.

T..9. The effluent released into the environment should have a high BOD and low
numbers of pathogens

T..10. Monitoring is carried out to quantify indicators of nonpathogens and BOD to make
sure the environment is safe for humans and wildlife.

T.11. Some nitrogen-fixing bacteria live free in the soil.

T.12. Microorganisms of decay break down the molecules in excretions and dead
organisms into nitrogen.

TUTORIAL 3

1. List THREE important microbes in the sewage treatment plant.

I) ____Algae__________

II) ____Cyanobacteria__________

III) ___Protozoa__________

2. In sewage treatment and environmental monitoring:

Define indicator organisms.

Types of organism used to detect and estimate the level of faecal contamination of
water
 Name TWO common indicator bacteria.

Total coliform

Faecal coliform

3. List the FOUR processes participate in the cycling of nitrogen through the biosphere
involving microorganisms.
- Mineralisation
- Nitrification
- Denitrification
- Assimilatory nitrate reduction

4. Bioremediation is:

a. The use of living organisms or their products to degrade waste into less
toxic/non-toxic products.
b. A Human made problem.
c. Natures way of cleaning itself.
d. The use of algae or their products to degrade waste into better products.
e. None of the above.

5. Which one of the following describes all existing bacteria?

a. Pathogenic, omnipresent & morphologically diverse

b. Extremophiles, tiny, abundant
c. Small, harmful & fast-growing
d. Tiny, ubiquitous, metabolically active
e. Morphologically diverse, metabolically diverse, extremophiles

6. Fill in the blank...

_______ soil treatment is the process of removing soil from the ground and treating it
at an outside location, such as a bioreactor.

A.Chemical
B.Physical
C.In Situ
D.Ex Situ