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Alginate and T Cells Proposal

Tiffany Mckenzie
Abstract
To stop deaths caused by HIV the body must learn to cope with it. By genetically
altering the target of HIV it will stop it from spreading. The target must be altered
in a way to prevent HIVs complicated process from happening by putting up a
barrier to prevent the target from becoming infected. By considering another
anatomy of another biological structure a successful barrier could come to mind.
Introduction
HIV attacks directly at the immune system, disrupting the way the body naturally
fights of infections.
This proposal is to use a different form of gene therapy to successfully prevent
HIV from inserting its genetic information into t cells.
The approach is to stop all forms of HIV from entering in any of the receptors it is
capable of entering. This will be done by stopping the lipid-lipid interaction
between HIV and the t cells by constructing a barrier.
What makes this study important is that it could potentially lead to the
development of a way to completely eliminate a common and deadly terminal
illness.
Method
Materials:
Hematopoietic stem cells
Pseudomonas aeruginosa PAO1 bacteria
Molecular scissors
Procedures:
1. Extract alginate genes algD, algA, algG, alg44, algF, algL, and etc. from
bacteria DNA using molecular scissors.
2. Extract and make copies of the genes.
3. Use gene design to make it so that the gene can function in the
lymphocyte.
4. Insert the gene into the hematopoietic cells using a gene gun.
5. Inject stem cells into the bone marrow of HIV positive patients.
Gene sequences for alginate genes
Pseudomonas aeruginosa , strain PAO1
AlgU

1 atgctaaccc aggaacagga tcagcaactg gttgaacggg tacagcgcgg agacaagcgg

61 gctttcgatc tgctggtact gaaataccag cacaagatac tgggattgat cgtgcggttc

121 gtgcacgacg cccaggaagc ccaggacgta gcgcaggaag ccttcatcaa ggcataccgt

181 gcgctcggca atttccgcgg cgatagtgct ttttatacct ggctgtatcg gatcgccatc

241 aacaccgcga agaaccacct ggtcgctcgc gggcgtcggc caccggacag cgatgtgacc

301 gcagaggatg cggagttctt cgagggcgac cacgccctga aggacatcga gtcgccggaa

361 cgggcgatgt tgcgggatga gatcgaggcc accgtgcacc agaccatcca gcagttgccc

421 gaggatttgc gcacggccct gaccctgcgc gagttcgaag gtttgagtta cgaagatatc

481 gccaccgtga tgcagtgtcc ggtggggacg gtacggtcgc ggatcttccg cgctcgtgaa

541 gcaatcgaca aagctctgca gcctttgttg cgagaagcct ga

AlgD

1 atgcgaatca gcatctttgg tttgggctat gtcggtgcag tatgtgctgg ctgcctgtcg

61 gcacgcggtc atgaagtcat tggtgtggat gtctccagca ccaagatcga cctgatcaac

121 cagggcaagt cgcccatcgt cgaaccgggc ctggaagcgt tgttgcagca aggccggcag

181 accggacggc tgtcgggcac caccgacttc aagaaggccg tgctggactc cgacgtatcg

241 ttcatctgcg tcggcacgcc gagcaagaag aacggcgacc tggacctggg ctacatcgag

301 accgtctgcc gcgagatcgg cttcgccatc cgcgagaagt ccgaacgcca caccgtggtg

361 gtgcgcagca ccgtactgcc gggcaccgtc aacaacgtgg tgatcccgct gatcgaggac

421 tgctcgggca agaaggccgg ggtcgacttc ggcgtcggca ccaaccccga attcctccgc


481 gagagcaccg cgatcaagga ctacgacttc ccgccgatga ccgtgatcgg cgaactggac

541 aagcagaccg gcgaccttct cgaggaaatc taccgcgagc tggacgcgcc gatcatccgc

601 aagaccgtcg aggtcgccga gatgatcaag tacacctgca acgtctggca cgccgccaag

661 gtcaccttcg ccaacgagat cggcaacatc gccaaggcgg tcggcgtcga cggccgcgag

721 gtgatggacg tgatctgcca ggaccacaag ctcaacctgt cgcgctacta catgcgtccc

781 ggcttcgcct tcggcggctc ctgcctgccc aaggatgtac gcgccctcac ctatcgcgcc

841 agccagctgg acgtcgagca cccgatgctc ggttcgttga tgcgcagcaa ctccaaccag

901 gtgcagaagg ccttcgatct catcaccagc cacgacaccc gcaaggtcgg cctgctcggc

961 ctgtcgttca aggccggcac cgacgatttg cgcgaaagcc cgctggtgga gctggccgag

1021 atgctcatcg gcaagggcta cgagttgcgc atcttcgacc gcaacgtcga atacgcgcgt

1081 gtccacgggg ccaacaagga atacatcgag tcgaagatcc cgcacgtctc ctcactgctg

1141 gtctccgacc tcgacgaagt ggtggcgagt tccgatgtgc tggtgctggg caatggcgac

1201 gagctgttcg tcgacctggt gaacaagacc ccgagcggca agaagctggt cgacctggtg

1261 ggcttcatgc cgcacaccac cactgcccag gccgagggca tctgctggta g

AlgA

1 atgatcccag taatcctttc cggtggcagc ggctcgcgac tctggcctct ttcccgcaag

61 cagtacccca agcagttcct cgccctcacc ggcgacgaca ccctgttcca gcagaccatc

121 aagcgcctgg ccttcgacgg catgcaggca ccgctgctgg tgtgcaacaa ggagcaccgc

181 ttcatcgtcc aggaacagct ggaggcacag aacctggcga gccaggcgat cctcctcgaa

241 cccttcggcc gcaacacggc gccggcggtg gccatcgccg cgatgaaact ggtcgccgaa

301 ggccgcgacg aactgctgct gatccttccc gccgaccacg tgatcgagga ccagcgcgcc

361 ttccagcagg ccctggcgct ggccaccaac gccgccgaaa agggcgagat ggtgctcttc

421 ggcattcccg ccagccgccc cgagaccggc tacggctaca tccgcgcgag cgccgatgcg

481 caactgccgg aaggcgtcag ccgggtgcag agcttcgtcg agaagcccga cgaagcccgc

541 gcccgcgagt tcgtcgccgc cggcggctac tactggaaca gcggcatgtt cctgttccgc

601 gccagccgct acctggaaga actgaagaag cacgacgccg acatctacga cacctgcctg

661 ctggccctgg agcgcagcca gcacgacggc gacctggtga acatcgacgc cgccaccttc

721 gaatgctgcc cggacaactc catcgactac gcggtgatgg agaagacctc acgcgcctgc

781 gtggtgccgc tgtccgccgg ctggaacgat gtcggcagct ggtcgtcgat ctgggacgtg


841 cacgccaagg acgccaacgg caacgtcacc aagggcgacg tgctggtcca cgacagccac

901 aactgcctgg tgcacggcaa cggcaagctg gtctcggtga tcggcctgga ggacatcgtg

961 gtggtggaaa ccaaggacgc catgatgatc gcccacaagg accgggtgca ggacgtcaag

1021 cacgtggtca aggacctcga cgcccagggc cgcagcgaga cccagaacca ctgcgaggtc

1081 taccgcccgt ggggctccta cgactcggtg gacatgggcg gccgcttcca ggtcaagcac

1141 atcaccgtga agcccggcgc gcgcctctcg ctgcagatgc accaccaccg cgccgagcac

1201 tggatcgtgg tttccgggac cgcccaggtg acctgcgacg acaagacctt cctgctcacc

1261 gagaaccagt cgacctacat cccgatcgcc tccgtgcacc gcctggccaa ccccggcaag

1321 atcccgctgg agatcatcga ggtgcagtcc ggcagctacc tcggcgagga cgacatcgag

1381 cgcctggaag acgtctacgg gcgcaccgca gaaccggccc tgcaagtggt cgccggcagc

1441 cgctga

AlgG

1 atgcccgaca tttccctttc gatcccccgc cgccggctcc cgcgcctgcg cccgctggcc

61 gcggcagtgc tcggcgcggt gctgctgcac ggccaggcgt gggcggcgca gccggtggag

121 aaaccgcagc cggtcccggc gcaggccggc aacgagccgg gcctgaccca gggcctgaag

181 gaaaccggca actacacggt gaccaccgcg ccggccgaac cgttgcacct ggacccgccg

241 aaactgcccg acctctccgg ctacaccgcc gccgcggtgg aagccaagat cgtgcgcaag

301 ccgggtggcc gcgccagcgt gcagcgcatg gtccagcagc agccgctgaa ggagttcacc

361 ggtggcagca accgcctcgc cgaatgggtc aagcgccagc gccagatgcc ccaggcgatc

421 ttcatcgaag gcggctacgt gaacctcgcc cagctcgccg gcaagcttcc cgccagcgcg

481 ctggaacagg tcgagccggg ggtgttcgtc gcccgcctgc cgatcgtggt cagccagggc

541 gcgaccctcg acatcgacaa gcaggtcaag gaactgcgcc tgtcccagga gcgcggcgcg

601 ttcctggtca acgacggcat gctgttcgtc cgcgacagca aggtcaccgg ctggagcgaa

661 tcgaagaagg agccggcctg gttcaagacg cccaacgagt tccgtccgtt cctgatctcc

721 tggggcggcg ccgaggtgta cctgagcaac agcaccttca ccagcttcgg ctacaacgcc

781 tccaaggcct acggcatctc catctcgcag tacagcccgg gcatggacaa gcagatgaag

841 cgcccgcgcc ccaagggctg ggtgatcgac tcgaccatcg tcgacagctg gtacggcttc

901 tattgctacg aggccgacga cctggtggtg aagggcaaca cctaccgcga caacatcgtc

961 tacggcatcg acccgcacga ccgttcgcac cgcctgatca tcgccgacaa caccgtccac


1021 gggaccagga agaagcacgg catcatcgtc tcgcgggaag tcaacgacag cttcatcttc

1081 aacaaccgca gctacgagaa caagctttcc gggatcgtcc tcgaccgtaa cagcgagggc

1141 aacctggtgg cctacaacga ggtctatcgc aaccattccg acggcatcac cctctacgag

1201 agcggcgaca acctgctctg gggcaaccag gtcctggcca accgccgcca cggcatccgc

1261 gtgcgcaaca gcgtgaacat ccgcctctac gagaacctcg cggcgggcaa ccagctgatc

1321 ggcgtgtacg gccacatcaa ggacctcacc aacaccgacc gcaacatcgc cctcgaccca

1381 ttcgacacca aggtctcgct gatcgtggtc ggcggcaagc tcgccggcaa cggctccggc

1441 ccgctctcgg tggattcgcc gctgagcctc gaactgtacc gggtggcgat gctcgccccg

1501 accaaatcct ccggcatcag cctgcccggc gttctcggcg agaagcagga ccagatcctc

1561 gacctgctgg tgcgccagga caaggcggtg ctgatcgacc ccgtggaaag ccaggccgaa

1621 ctccaggact ga

Alg44

1 atgaatacag ccgtcaacgt caacgtcgtg cacgaatccg aagcccagcg ccagttcgcc

61 cgggtcaagc tgccggcgcg catccgctac atcggcgcca accgcgaagg cgtcgacgca

121 cgcctgctgg atctctccgc cggcggcttc gccttcaccg cgagcggcgc gccgatccag

181 cccggcgacc tgtacaaggg caagctgctg ttccaggtcg acagcatcag cttttccctc

241 gaagtggagt tccaggtgcg ctcggtggac cccgccagcc gccgcgtcgg ctgcgagttc

301 cagaacctca agccgcgcga ggtggcggcc ctgcgctacc tgatcacctc ctacctggcc

361 ggcgaggtga tcggcgtcgg cgacatgctc aataccctcc agcgcgagaa cttcaccaag

421 gcccgcaagc agggcggcgg caacggcggg atgggcttct tcggccgggt ccgggcggtg

481 accctgagca ccgcgatctt cgtcgtcggg gtcggcgcct tcgccttcat cctcaaccag

541 atgtacaacc tgtacttcgt cacccatgcc gattccggcg tggtcagcgt ccccaaccag

601 cagatcacca tgccccgcga gggcaccgtg cagagccttc tcgggcccaa cgcggaagtc

661 gccaaaggcg cgccgatcgc taccttctcg gccaacctgc tggacatgct caagggcaac

721 ctgaccgagg agcagctcaa cccgggcaac atcgagaagc tcttcggcca ccagatgaaa

781 gggaccctga ccagcccctg cgactgccgc gtggtccagc aactggtagc cgacgggcaa

841 tacgccaaca agggccaggt gatcttcacc ctcgcgccgc gcgacagcgt ggccagcatc

901 gaggcgcgct tcccctaccg caacgccgcc gagctggcgc cgggcacccg ggtgaacttc

961 caggtcgccg gcgacggcgt caaccgcagc ggccgcatcg tcaacaccgc gccggtggac


1021 ggcgacctgt cctcggaaat ccgcgtgcag atccagcccg accagccgct cgacgcccag

1081 tacgccggcc gcccggccga ggtcagcatc ggcggcctgc ccggccgcac cctgctgaac

1141 aaggccgtga ccctggccac cgctcgctga

AlgF

1 atgaacccga tgacccgccg ccacacctgg acccgcctgg cctgcgcact ttccctcggc

61 gtcgccgcat tcgccgcgca ggccgacgag ggcgccctgt acgggcccca ggcacccaag

121 ggctcggcct tcgtccgcgc ctacaacgcc ggcaacagcg aactcgacgt gtcggtcggc

181 agcaccagcc tgaacgacgt cgcgccgctc ggttccagcg acttcaagtt cctccccccc

241 ggcagctaca ccgcccaggt cggccagcag agcctgccgg tcaagctcga cccggacagc

301 tactacaccc tggtcagcca gcccggcggc aagccgcagc tggtggccga gccgccgttc

361 aagaacaagc agaaggcgct ggtacgggtg cagaacctca gcggctcgaa gctgaccctg

421 aagaccgccg acggcaagac cgacgtggtc aaggacgtcg gcccgcaaag ccacggcgac

481 cgcgagatca acccggtgaa ggtgaacctg gcgctgttcg acggcagcaa gaaggtcagc

541 gacctgaagc cagtcaccct ggcccgcggc gaggtggtct gcctgtacgt caccggcagc

601 ggcggcaagc tcgcgccggt ctgggtcaag cgcccggtga aggcggactg a

AlgL

1 atgaaaacgt cccacctgat ccgtatcgcc ctgcccggtg ccctcgccgc ggcattgctc

61 gccagccagg tcagccaggc cgccgacctg gtacccccgc ccggctacta cgcggcggtc

121 ggcgagcgca agggcagcgc cggcagctgc cccgcggtgc cgccgccgta taccggcagc

181 ctggtcttca ccagcaagta cgaaggctcc gattcggcgc gggcgaccct caacgtcaag

241 gcggagaaga ccttccgctc gcagatcaag gacatcaccg acatggagcg cggcgccacc

301 aagctggtca cccagtacat gcgcagcggc cgcgacggcg acctggcctg cgcactgaac

361 tggatgagcg cctgggcccg cgccggcgcc ctgcagagcg acgacttcaa ccacaccggc

421 aagtccatgc gcaaatgggc gctgggcagc ctctccggcg cctacatgcg cctgaagttc

481 tccagctcgc ggccgctcgc ggcccacgcc gagcagagcc gggaaatcga ggactggttc

541 gcccggctcg gcacccaggt agtccgcgac tggagcggcc tgccgctgaa gaagatcaac

601 aaccattcct actgggcggc ctggtcggtg atgtccaccg cggtggtgac caaccgccgc

661 gacctcttcg actgggcggt gagcgagttc aaggtcgccg ccaaccaggt cgacgagcag

721 ggcttcctgc ccaacgaact caagcgccgc cagcgcgccc tcgcctacca caactatgcg


781 ctgccaccgc tggcgatgat cgccgcgttc gcccaggtca acggcgtcga cctgcgccag

841 gagaaccacg gcgccctgca gcgcctggcc gagcgggtga tgaagggagt cgacgacgag

901 gaaaccttcg aggagaagac cggcgaggac caggacatga ccgacctcaa ggtcgacaac

961 aagtacgcct ggctggagcc ctactgcgcc ctctaccgct gcgagccgaa gatgctcgag

1021 gcgaagaagg accgcgagcc gttcaacagt ttccgcctcg gcggcgaagt gacgcgggtg

1081 ttcagccgcg aagggggaag ttga

Alg8

1 atgatggaaa cttacaaacg tggcctcgcc gaagccaccg gctggctggt gttcctcagc

61 ctgctgatgg tgctcgcgct ggcagtgccg aagaccgtgt tcgacgccga ctccaaggat

121 ttcatcctgc ttatcggcgc cgtcggcatc tggcgctact ccatgggcgg cgtgcacttc

181 ctgcgcggca tgctgttcct ccacgtggtc tacccgtact accgccggcg cgtgcgccag

241 ttgggcagcg cggccgaccc gtcgcacgtg ttcctgatgg tcaccagttt ccgcatcgac

301 gccctgacca ctgccatggt ctatcgctcg gtgatccgcg aagccatcga cagcggctac

361 ccgaccaccg tggtctgctc catcgtcgag atgtccgacg aggtcctggt ccgttctctg

421 tgggagaaga tgaacccgcc ggaccgggtc agcctcgact tcgtgcggat tcccggcacc

481 ggcaagcgcg acggcctggc ctatggcttc cgcgcgattt cccggcacct gccggacgac

541 gacgcggtgg tcgcggtgat cgacggcgac accgtgctcg accacggcgt ggtgaagaag

601 accgtgccct ggttcaagct cttccccaac gtcggcggcc tcaccaccaa tgaattctgc

661 gaagtgcagg gcggctacgt gatgagcgag tggcacaagc tgcgcttcgc ccagcgccac

721 atcaacatgt gctcgatggc cctgtccaag cgcgtactga ccatgaccgg acggatgtcg

781 gtgttccgcg cccgggtggt gaccaacccc gagttcatca ccgacgtcga gaacgaccac

841 ctggagcact ggcgcctggg tcgcttcaag ttcctcaccg gcgacgacaa gtccagctgg

901 ttcagcctga tgcgcctggg ctacgacacc ttctacgtgc ccgacgcggc gatcaacacg

961 gtcgagcacc cgccggagaa gagcttcatc aaggccagtc gcaagctgat gtaccgctgg

1021 tacggcaaca acctgcggca gaactcgcgc gcgctcaagc tcggcgcgcg gcgcctgggc

1081 tggttcacca tgctggtgct gttcgaccag cgcgtctcga tgtggaccag cctgctcggc

1141 ctggtggtgg cgatcctcgc cagcctcaag tacagcatcg ccttcctgct ggtgtacctg

1201 ctctggatcg gcctcacccg cctggtgctg accctgctcc tctcgctctc cgggcaccgc

1261 atcggcccgg cctatccgct gatcctctat tacaaccaga tcgtcggcgc gctggtgaag


1321 atctacgtgt tcttccgcct cgaccggcag tcctggaccc gccagccgac caagctggag

1381 cgcggcctgg ccagcttcca gcgctggttc aacgcctggt cgtcgcgggc catgaccttc

1441 tctgccgcca gcatcttcgt cgccgtgctg ctgaccatcg tatga

AlgK

1 atgaagatgc ccatcctccc tcccctgccc ctggcgtccc ggcacctgct gctggcctcg

61 gccatcgccc tggccgccgg ctgcgccgga ctgcccgacc agcgcctggc ccaggaggcc

121 ctggagcgcg gcgacctcgc caccgcccag agcaactacc aggccctggc cgcgatgggt

181 tacgccgacg cccaggtcgg cctcgccgac atgcaggtcg ccagcggcga cagcgcccag

241 caggccaagg ccgaaaagct ctaccgcgag gccgcgcaga cctcgccgcg ggcccgcgcg

301 cgccttggca agtggctggc ggccaagccc ggcgccagcg acgccgagca ccgcgaggcc

361 gagcgcctgc tcagccaggc cttcgaacag ggcgaggaca gtgccctggt gccgctcatc

421 gtgctctacc tgcaataccc gcagtcctgg ccggaaatcg acccgcagca gcgcatcgac

481 cagtggcgcg cgcgcggact gccgcaggcc gacctggcgc agatcatcct ctaccgcacc

541 cagggcacct acgcccagca cctgggcgag atcgagcagg tctgccagcg ctggctgcgg

601 cgcatggacg tgtgctggta cgaactggcc acggtctacc agatgcaggg caacgcggaa

661 aagcagaagg tcctgctcga acagctgcgc gccgcctaca aggccggccg ggtgcccggc

721 gagcgggtcg actcggtggc cggggtgctc gccgacggcg aactcggcca gcccgacccg

781 cagaccgccc aggcgctgct ggaagagatc gcgccgagct accccgcggc ctgggtcagc

841 ctggccaagc tgctctacga ctatcccgac cagggcgacc tggaaaagat gctcggctac

901 ctgaagaacg cccaggacgc cgcccagcca cgcgccgaac tgctcctcgg gcgcctctac

961 tacgacggca agtgggcgcc gcaggacccg cgcaaggccg agcggcacct gctcaaggcc

1021 gccgccagcg aaccccaggc caactactac ctggggcaga tctaccgccg cggcttcctc

1081 ggcaaggtct atccgcagaa ggccgtcgac cacctgatcc tcgccgcccg cgccggccag

1141 gccagcgccg acatggccct ggcccagctc tggtcgcagg gccgcggcat ccagccgaac

1201 cgggtcaacg cctacgtctt cggccagctc gccgtgcagc agcaggtacc gcaggccagc

1261 gacctgctcg ggcagatcga agcgcaactg ccgccggccg aacgcagcca ggcgcagcaa

1321 ttgctcaagc gcgaacaaca gagccgcggc aacaactggc aggccaccgt cagcctgctg

1381 cagagccagg actcgccgat caacgaagaa gagccagaaa gcctatga

AlgE
1 atgaacagct cccgttccgt caacccgcgg ccgtccttcg cgccgcgcgc cctgtccctg

61 gccatcgccc tgctgctcgg cgcgccggcg ttcgccgcca acagcggcga ggcgccgaag

121 aacttcggcc tggacgtgaa gatcaccggc gaatcggaaa acgatcgcga cctcggcacc

181 gctcccggcg gcaccctcaa cgacatcggt atcgacctgc ggccctgggc cttcggccag

241 tggggcgact ggagcgccta cttcatgggc caggcggtgg ccgccaccga caccatcgag

301 accgacaccc tgcaatcgga caccgacgac ggcaacaaca gccgcaacga cggtcgcgag

361 ccggacaaga gctacctcgc cgcacgcgaa ttctgggtcg actacgccgg cctcaccgcc

421 taccccggcg agcacctgcg cttcggccgc cagcgcctgc gggaagacag cggccagtgg

481 caggacacca acatcgaggc gctgaactgg agcttcgaga ccaccctgct caacgcccac

541 gccggggtcg cccagcgttt cagcgaatac cgcaccgacc tcgacgaact ggctccggag

601 gacaaggacc gcacccatgt gttcggcgac atttccaccc agtgggcgcc gcaccaccgc

661 ataggcgtgc gcatccacca cgccgacgac agcggccacc tgcgccgccc cggcgaggaa

721 gtcgacaacc tcgacaagac ctataccggc cagctcacct ggctcggcat cgaggctacc

781 ggcgatgcct acaactatcg ttcgagcatg ccgctgaact actgggccag cgccacctgg

841 ctgaccggcg accgcgacaa cctgaccacc accacggtcg acgaccggcg catcgccacc

901 ggcaagcaga gcggcgacgt caatgccttc ggcgtcgacc tcggcctgcg ctggaacatc

961 gacgagcaat ggaaggccgg cgtcggctac gcccgcggca gcggcggtgg caaggacggc

1021 gaggagcagt tccagcagac cgggctggag agcaatcgct ccaacttcac cggcacccgc

1081 tcgcgcgtgc accgcttcgg cgaagccttc cgcggcgaac tgagcaacct ccaggcagcc

1141 accctgttcg gctcctggca actgcgcgag gactacgacg cgagcctggt ctaccacaag

1201 ttctggcgcg tcgacgacga ctccgacatc ggtaccagcg gcatcaacgc cgccctgcaa

1261 ccgggcgaga aggacatcgg ccaggaactc gacctggtgg tgaccaagta cttcaagcaa

1321 ggcctgctgc cggcctcgat gagccagtac gtcgacgagc cctcggcgct gatccgcttc

1381 cgcggcggcc tgttcaagcc gggcgacgcc tacgggccgg gcaccgactc gaccatgcac

1441 cgcgccttcg tcgacttcat ctggcgcttc tga

AlgX

1 atgaaaaccc gcacttcccg actgttccgc ctgagcgccc tggccgccgg cctctgcctg

61 gcccaggcag ccctggccgc cgaccccggc gcggcgccga gctaccaggc gctgccggcc

121 ggcaacctgt gcccggccgc cgcctacgac tcgcgctaca acaccaagta cctgggcttc


181 ttcacccacc tggtgcaggc ccaggacgac tggctgttcc gcaccaccta cgacctgcgt

241 accgacttcg gcaccagcgc cgaaggctgg cgcgaactcc gcgcactgcg cgacgaactc

301 aagcgcaagg gcatcgagct ggtggtggtc taccagccga cccgcggcct ggtgaaccgc

361 gagaagctca gcccggcgga aaaggccggc ttcgactacg agctggcgaa gaagaactac

421 ctggcgacca tcgcccgctt ccgccaggcc ggcatctgga ccccggactt ctcgccgctg

481 ttcgacgaga aggaggagca cgcctactac ttcaagggcg accaccactg gactccccat

541 ggcgcccggc gcagcgcgaa gatcgtggcc gagacgctga agcaggtgcc cggcttcgag

601 gagatcccga agaagcagtt cgaaagcaag cgcgtcggcc tgctgtccaa gctcggcacc

661 ttccacaagg ccgccgcgca actctgcggc aacagctacg ccacccagta cgtcgaccgc

721 ttcgagaccg agccggtggg cgccagcgac agcggcgacc tgttcggcga cggcggcaac

781 ccgcagatcg ccctggtcgg cacctccaac agcggcccgg cctacaactt cgccggcttc

841 ctggaggagt tcagcggcgc cgacatcctc aacaacgcgg tgtccggcgg cggcttcgac

901 agttcgctgc tggcgtacat gaccagcgag gaattccaca agaacccgcc gaagatcctc

961 atctgggaat tcgccaccca ctacgacatg gcgcagaaga gcttctaccg ccaggccatg

1021 ccgctggtgg acaacggctg ctccggccgg aagaccgtac tcagccgcaa ggtcaagctg

1081 cgccagggcc gcaacgaggt gctgctgaac agcgccgcgc taccgatccg tagcggcagc

1141 tacgtcgccg acgtgaccta cagcgaccct tcggtacacg agttgaagaa caccatctgg

1201 tacatgaacg gccgccgcga gcagttgaag atcgagcagt cgaaagccgt cgataccggc

1261 ggccgctacg tcttccagct gcgcaacgac agcgactggg ccgaccagca gttcctttcc

1321 ctggaaatcg aagcgccgga ggacatgccc cagggcctgg aggtccaggc cagcatctgc

1381 caggcggcgc ccgccaaggc cagccagtcg gtggccggga ggtaa

AlgJ

1 atgacacaga gcatttcccg ccccctgcaa tacgcctata tcgccgcctt cggcgggctg

61 ctgctggggc tcgccggatg gtcgctgaag agcgtgccgg gcttctccgc cgcggccgat

121 acgccactgc tcaacggcaa gctggcgcac gccttcgaag cccactacga caaggaattc

181 ccgatcaagc gcctgggcac caacctctgg gccgcactcg actacacgct gttccacgaa

241 ggccgccccg gcgtggtgat cggcaaggac ggctggctgt tcaccgacga agagttcaag

301 ccggcaccga gcggccagca actggaagac aactgggcac tggtacgcgg cgtgcagcgg

361 gagctgaatc ggcgcggcgt caagctggtg ctcgcggtga tcccggccaa ggcccgcctg


421 tatcccgagc acatcggccg cgagcagccg gccgcgctgc acgacagcct ctaccaggac

481 ttcctggccc gcgcccgcgc cgccggcatc gacagtcccg acctgctcgg cagcctgcgc

541 caggccaagg acaacggcgc ggtgttcctg cgcaccgaca cccactggtc gccgctcggc

601 gcggaaaccg tggcccagcg cctcggcgcg gagatccgcg agacgcacct gctggatgtg

661 ccggcgcaga acttcgtcac ccgcgtcggc gaagaacgca cgcacaaggg cgacctgctc

721 agcttcctgc cgctggaccc gctgttcgac gaactgctgc cgcgtccgga gcagttgcag

781 caacgcacca ccgaagccgc gccggcactg ccgggcggcc agcagtccgg cgcgggcgac

841 gacctcttcg gcgatagcca gcaaccgcgc ctggcgctgg tcggcaccag ctacagcgcc

901 aaccccaggt ggaacttcga gggcgcgctg aaacaggcgc tgtccgccga cctgatcaat

961 tacgcgaagg aaggcaaggg ccccctcgaa ccgatgctgg aactgctgca ggacgaaggc

1021 ttccgcaagg acccgccgca actgctggtc tgggagtttc ccgagcgcta cctgccgatg

1081 gccagcgacc tcagccagtt cgacgccgac tgggtcgccc agctgaaggc cagcggcggc

1141 cgcgatgagc gcctggccgc cagccgcaac gattga

AlgI

1 atggtctttt cttcaaacgt gttcctgttc ctgttcctgc cggtctttct cggcctgtac

61 tacctgagcg gcgaacgtta ccggaacctg ctgctgctga tcgccagcta cgtgttctac

121 gcctggtggc gggtggactt cctcctgctg ttcgccgggg tcaccgtctt caactactgg

181 atcggcctgc gcatcggcgc cgccggcgta cgcaccaggg ccgcgcaacg ctggctgatc

241 ctcggcgtgg tggtcgacct ctgcgtgctc gggtacttca agtacgccaa cttcggcgtc

301 gacagcctca acgagatcat cacctcgttc ggcatgcagc cgttcgtgct gacccacatc

361 ctgctgccga tcggcatctc cttctacacc ttcgagtcga tcagctacat catcgacgtc

421 taccgcggcg ataccccggc gacccacaac ctgatcgact tcgccgcctt cgtcgcgatc

481 ttcccgcacc tgatcgccgg cccggtgctg cgcttcaagg acctggtcga ccagttcaac

541 caccgcaccc acaccgtcga caagttcgcc gaaggctgca cccgcttcat gcagggcttc

601 gtcaagaagg tgttcatcgc cgacaccctg gccgccctcg ccgaccactg cttcgccctg

661 cagaacccga ccaccggcga cgcctggctc ggcgccctgg cctacaccgc gcagctctac

721 ttcgacttct ccggctacag cgacatggcc atcggcctcg gcctgatgat gggcttccgc

781 ttcatggaga acttcaacca gccctacatc agccagtcga tcaccgagtt ctggcggcgc

841 tggcacatca gcctgtcgac ctggctgcgc gactacctct acatcagcct gggcggcaac


901 cgcggcagca ccttccagac ctaccgcaac ctgttcctga ccatgctcct cggcggcctg

961 tggcacggcg ccaacttcac ctacatcatc tggggcgcct ggcacggcat gtggctggcg

1021 atcgagcggg ccctcggagt gaacgccgcg ccgcgcgtgc tcaacccgtt gaagtgggtg

1081 atcaccttcc tcctggtggt gatcggctgg gtgatcttcc gcgccgagaa cctgcaggtc

1141 gcctggcgca tgtacgaagc catgttcagc ttcggcacct ggcagctctc cgaactgaac

1201 cgcgccaacc tcaccggcct gcaggtcggc accctggtcc tggcctacct ggtgctggcg

1261 ttcttcggcc tgcgccagtt ctacaaccag ccattgcaga ccaaggcgcc caaggccgcg

1321 gcgaacagcg acgaagtggc cgccgacggc ccggccagtg cccagccgcg tgccccgcgc

1381 gaggccgccg gcgacccggc ggcgatcgcc tactcgccga gcggcgcgct ggtctaccag

1441 ccgagctggc tcagccagtt gcccgtcctg gccacccgcc tggcgctgct cctgctgttc

1501 gccgcctcgg tgctgaagct ctccgcgcag agctactcgc cattcctcta cttccagttc

1561 tga

AlgW

1 atgcccaagg ccctgcgttt cctcggctgg cccgtgctgg tcggcgtgct gctggccctg

61 ctgatcatcc agcacaaccc cgagctggtc ggcctgccac gccaggaggt gcacgtcgag

121 caggcgcctc tgctcagccg cctgcaggaa ggcccggtgt cctatgccaa cgcggtgagt

181 cgagcggctc cggcagtggc caacctgtac accaccaaga tggtcagcaa gccctcccac

241 cccctgttcg acgacccgat gttccgccgc ttcttcggcg acaacctgcc gcaacagaag

301 cgcatggagt cgagcctcgg ctcggcggtg atcatgagcg cggaaggcta cctgctgacc

361 aacaaccacg tgaccgctgg cgccgaccag atcatcgtgg ccttgcgcga cggccgcgaa

421 accatcgccc agttggtcgg cagcgacccg gaaaccgacc tggccgtgct gaagatcgac

481 cttaagaacc tgccggcgat gaccctcggc cgctccgacg gcattcgcac cggcgacgtc

541 tgcctcgcca tcggcaaccc gttcggcgtc ggccagaccg tgaccatggg catcatcagc

601 gccaccggac gcaaccagct cggcctgaac acctacgaag acttcatcca gaccgacgcg

661 gcgatcaacc ccggcaactc cggcggcgcg ctggtggacg ctgccggcaa cctgatcggc

721 atcaacacgg cgatcttctc caagtccggc ggctcccagg gtatcggctt cgccatcccg

781 accaagctgg ccctggaggt catgcagtcg atcatcgagc acggccaggt gatccgcggc

841 tggctcggcg tcgaggtcaa ggcgctgacc ccggaactgg cggagtcgct gggcctcggc

901 gaaaccgccg ggatcgtcgt cgccggcgtc tatcgcgacg gtccggcggc acgcggcggc


961 ctgctgccgg gcgatgtgat cctgaccatc gacaagcagg aagccagcga cggccgccgc

1021 tcgatgaacc aggtggcgcg cacccgtccg ggacagaaga tcagcatcgt ggtgctgcgc

1081 aacggacaga aggtcaacct gaccgccgag gtcggcctgc gtccgccgcc ggcaccggct

1141 ccacagcaga aacaggacgg cggcgagtga

AlgP

1 ttaggaggcg ctggtcgggg cggcgccgtt gctgcccgcg gcaggcgttg ccgaagcagc

61 gctggaggcg gctgccgggg cgctgggagt cgccgcaggc ttcgcagcag gagcggtggc

121 gggcttggtt gcggcaggtt tcgcggctac gggtttcgcc gcaggcttgg ccgccggttt

181 cgccgcaggc ttcgcagccg gcttggctgc aggcttcgcg gccgcggatt tcgccacagg

241 cttggccgcc ggtttcgccg caggcttcgc agctggcttg gctgcgggct tcgcggctgc

301 ggttttcgcc gcgggcttgg ccgtcggttt cgcgacaggc ttcgctgccg gcttggcggc

361 gggcttcgcg gctgcggttt tcgccgcagg cttggccgcc ggtttcgcca caggcttcgc

421 cgccggcttg gctgcgggct tcgcggctgc ggttttcgcc gcgggcttgg ccgccggttt

481 cgcagcaggc ttcgccgtcg gcttggctgc gggcttcgct gccgcggttt tcgccgcagg

541 cttggccgcc ggtttcgcag caggcttcgc agccggcttg gccgcaggct tcgccgctac

601 ggttttcacc gcaggcttgg ctgccgcctt cgcagcaggt ttggtcgcgg gtttcgcctt

661 gcggctctcc agagccttgc cagcagcttc cttcaccttg ccgacaccct gcgccagctt

721 gaggctttcc tgtacgtcac gcttgaggcc gacgatgtag gtacgggtgt ccgcctgccg

781 ggccttcagg gtatccaacg cctcttccag gtcgctgatg gtctcgcgcg ccttggcctg

841 tgccttggtc ttgccggcct tggcagcatc ctgcagcttg gtgcgagcct tgtgcagctt

901 ttcctgggct ttgccacgtt gcttttcaag tttggccagg agcttttccg aatcgaccag

961 cgcttgtttg cacgcacctt ccaggtgctc gacaaggctg tgggaaagtt gctgcaacag

1021 gtgcaagggg gtggtgacgg gcttcttgtt ggccgacat

AlgQ

1 tcagaccggt actgccgaac cctctgcgcc gctctggctg tgggcgttgt gcaggacttc

61 gatgaggcag tcctccagct cgaagcgttc gtgcaactgt tgccgcagga ccttcagctc

121 cgcgatgagg caggctcctt cacggcaatc gccgttgtcg cagcggtcgt tgaagttcag

181 cgcggattcg gtgatggctt ccagccgggg gaagatctgc ttggccagct ccaggccgcg

241 ctgatcgccg aaggccttgc cttccgccgt cagttgctcg tagacctcga agtgtccggc


301 agatacatag tccagcagca attggcagaa atgctggagt tcttcggcgt tgggcgccgg

361 tgcctgaata ccgctcaggg catcgaaggc ctgcaccagt tgctggcgct catgcagcca

421 gcgatcgatc agttggtgca caccccccca ccgctcttgg gcattacggc agctttcgag

481 cat

AlgR

1 tcagagctga tgcatcagac gcctgactcc ggccacgtgc cgccggctga cggtcagcgc

61 atcgccgtcg aggcctttca ggtagagctg gaaatgcccc agcggcgtac gctgcaggcg

121 ttcgatccgt tcgcgggcga ccagcgcgtt gcggtggatg cgcacgaagc gctcgccgaa

181 ctcgtcttcc agcgccttca acggctcgtc cagcagcacc tcgccctgcg catggcgcaa

241 ggtcacgtac ttgtggtcgg caatgaagaa gatcacctct tccagcggga tcagctcgat

301 ccccttgcgg gtccgtgcac tgatgtggct gcgcggaccg ctgccgccgg aggccggggg

361 cttggtcagc gcggccagtt gcacgcggtt cggtcgcgag gctttcttca acgcctcggc

421 caggtcttcg ctgcgcaccg gcttgaccag gtagcccacg gcgctgacct ggaaggcttc

481 cagggcgaat tcgtcatggg ccgtgcagaa gatcacagcc ggcggcgctt cccgctcgca

541 gagtctggcc gcgacctgga ggccgtccag accgggcatg cggatatcca gcaggacgat

601 atcgggcttg aggctgtcga tcagcgtcag cgcttcttcg ccattgctgg ccgagggctc

661 gaggacgcga tagccgtcca gttgccctac caatcgggcc aggcgctctc gcgccagagg

721 ttcgtcatcg acaatcagga cattcat

AlgZ

1 tcaggcttcc tgcatgagtc gcgcacatgg atagcgtaga caggtgtagt gccgtccgtc

61 acggcgctcg acgctgagac tcgctttagg cccgaaaagt gccccaagtc gtgcatcgat

121 attgtgtaac gcctgtcgcg tgcctttcga cgggggcgac tcgagcgcct cgtcgtaggg

181 gttgctcaca catagctgga acactccttc acgataaacc gcctcgacct gcaccaggcc

241 gccttcgacc cgcggctgga tgccatagat aagcgcattt tccagcagcg gctgcaaggt

301 cagttggggg atcggcaggt tggccggcac cccgtgcacc tgccagtcca gttgcaggcg

361 atcgccaaga cgatactgct caatggaaag atagcgcctt gccagcgcca gttcctcctc

421 ccaggaaact aaagtgccgg gcttcgccag gctggcccgg aacaggtcgg acaggtccag

481 caccgcatgc tcggccttca gcgggtccag ctcgatcagg ctggcgatgc tgttcaggct

541 gttgaacagg aagtgcgggc gaatccgcgc ctgcagcgat tccaggcgcg cctgcaactc


601 ggcctgctgc tgccggcgcc actggctctg caggtaaaag tagcgcagca ccagggcgga

661 catgatcagc gcgatcagcg cgtgccgcag atacagattg acctcgccgg cacgcgtcag

721 ttctccgccc agctgatagt gctcggcgac cgccgtgcag cccagggtga gggccaccac

781 caacaggcag caggcactcc ccgccagcgc caccggcagg cgtgcgagca atggccgcag

841 gcggcagaac agcgccgccg agagcagtac gatccactgc acgaacagcg acgccagggc

901 caggcgcacc cagttgaagc tcggcgtcat cggctccgcc agcaccagca cgagcaccag

961 cagctccgcc agcagcacca gggtgaacag ggcttcggct tcgcagagct cgggaatgaa

1021 gaagtcgtct tggacggccg gccgggtcga gcggccgggc ttgaatcgga taggcat

AlgC

1 atgagcactg caaaagcacc gacgctgccc gccagcatct tccgcgccta cgacatccgt

61 ggcgtggtag gcgataccct caccgccgag accgcctact ggatcggtcg cgccatcggc

121 tcggaaagcc tcgcccgcgg cgaaccgtgc gtcgctgtcg gccgcgatgg ccgcctgtcc

181 ggtcccgagc tggtcaagca gctgatccag ggcctggtgg actgcggttg ccaggtcagc

241 gacgtgggca tggtgcctac cccggtgctg tactacgcgg ccaacgtgct cgagggcaag

301 tccggggtga tgctgaccgg cagccacaac ccgccggact acaacggctt caagatcgtg

361 gtcgccggag agaccctggc caacgagcag atccaggccc tgcgcgagcg catcgagaaa

421 aacgacctgg catccggcgt cggcagcgta gagcaggtcg acatcctgcc gcgctacttc

481 aagcagatcc gcgacgacat cgccatggcc aagccgatga aggtggtggt cgactgcggc

541 aacggcgtgg ccggggtgat cgccccgcag ttgatcgagg ccctgggctg cagcgtgatc

601 ccgctgtact gcgaggtcga cggcaacttc ccgaaccacc atccggaccc gggcaagccg

661 gagaacctga aggacctgat cgccaaggtc aaggccgaga acgccgacct gggcctggcc

721 ttcgacggcg acggcgatcg cgtcggcgtg gtcaccaata ccggtaccat catctatccg

781 gaccgtctgc tgatgctgtt cgccaaggac gtggtctcgc gcaacccggg ggccgacatc

841 atcttcgacg tcaagtgcac ccgccgtctg atcgccctga tcagcggcta cggcggccgt

901 ccggtgatgt ggaagaccgg ccactcgctg atcaagaaga agatgaagga aaccggcgcc

961 ctgctggctg gcgagatgag cggccacgtg ttcttcaagg agcgctggtt cggcttcgac

1021 gatggcatct acagcgccgc ccgcctgctg gaaatcctca gccaggatca gcgtgacagc

1081 gagcacgtgt tctcggcctt cccgagcgac atttccaccc cggaaatcaa catcaccgtc

1141 accgaggaca gcaagttcgc catcatcgag gcgctgcaac gcgacgccca atggggcgaa


1201 ggcaacatca ccaccctcga cggcgtgcgg gtcgactacc ccaaaggctg gggcctggta

1261 cgcgcctcca acaccactcc cgtgctggtc ctgcgcttcg aagcggacac cgaggaagag

1321 ctggagcgca tcaagaccgt cttccgtaac caactgaagg cagtggattc ctcgctgccc

1381 gtgcccttct ga

AlgB

1 atggaaacca cttccgaaaa acaggggcgc atcctgctgg tcgatgacga gtcggcgatc

61 ctgcgcactt tccgttattg cctcgaagac gaaggctaca gcgtggccac cgccagcagc

121 gcgccgcagg cggaggccct gttgcagcgc caggtattcg acctgtgctt cctcgacctg

181 cgcctgggcg aagacaacgg gctcgacgtt ctcgcccaga tgcgcgtcca ggcgccatgg

241 atgcgcgtgg tgatcgtcac cgcgcattcg gcggtggata ccgcggtcga tgccatgcag

301 gccggcgcgg tggattacct ggtcaagccc tgcagcccgg accaactgcg cctggccgcc

361 gccaagcaac tggaggtgcg ccaactgacc gcgcgcctgg aggccctgga ggacgaagtg

421 cgccgccagg gcgacggcct ggaatcgcac agcccggcca tggccgcggt actggagacc

481 gcgcgccagg tagcggcgac cgacgccaac atcctcatcc tcggcgaatc cggctccggc

541 aagggcgaac tggcacgcgc catccacacc tggagcaaac gcgcgaagaa gccccaggtc

601 accatcaact gcccgtcgct gaccgccgaa ctgatggaaa gcgaactgtt cgggcacagt

661 cgcggcgcct tcaccggtgc caccgaaagc accctgggca gggtcagcca ggctgacggc

721 ggcaccctgt tcctcgacga gatcggcgac ttcccgctga ccttgcaacc caagctgctg

781 cgcttcatcc aggacaagga atacgaacgc gtcggcgatc cggtgacccg ccgcgccgac

841 gtacgcatcc ttgccgcgac caaccgcgac ctgggcgcga tggtcgccca gggccagttc

901 cgcgaggacc tgctctaccg cctcaacgtg atcgtgctca acctgcctcc cctgcgcgaa

961 cgcgccgagg atatcctcgg cctggccgaa cgtttcctcg cccgcttcgt caaggactac

1021 ggccgccccg cccgcggctt cagcgaagcc gcccgcgagg ccatgcggca atacccctgg

1081 ccgggcaacg tacgcgagct acgcaacgtg atcgaacgcg ccagcatcat ctgcaaccag

1141 gaactggtgg atgtcgacca cctcggtttc agcgctgcgc aatctgccag cagcgcgccg

1201 cggatcggcg aatcgctgag cctggaagac ctggagaaag cccatatcac ggcggtgatg

1261 gcctccagcg cgaccctcga ccaggccgcc aagaccctcg gtatcgatgc ctcgaccctg

1321 taccggaagc gcaagcagta cggcctatga


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https://www.nature.com/scitable/topicpage/cell-membranes-14052567

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https://www.ncbi.nlm.nih.gov/nuccore/AE004091.2