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Article history: An algae biolm growth system was developed to study the growth kinetics and neutral lipid productiv-
Received 17 December 2012 ities of Scenedesmus obliquus and Nitzschia palea, and to determine if algal biolms can be starved of key
Received in revised form 5 March 2013 nutrients to increase their neutral lipid concentrations. Linear growth curves were determined for each
Accepted 7 March 2013
species until nutrient starvation commenced, at which point growth ceased and/or biolms sloughed
Available online 15 March 2013
from their substratum. Nutrient starvation did not increase neutral lipid concentrations in any of the bio-
lms; however, it approximately doubled their lipid concentrations when grown in suspension. Biomass
Keywords:
productivities of 2.8 and 2.1 g/m2/d and lipid productivities of 0.45 and 0.18 g/m2/d were determined for
Biofuels
Algae
N. palea and S. obliquus, respectively. The results suggest that nutrient starvation of biolms is not a desir-
Biolms able method of lipid production for algae biolm biofuel production systems, but that lipid production
Lipids rates compare favorably with conventional terrestrial biofuel sources.
Nutrients 2013 Elsevier Ltd. All rights reserved.
1. Introduction cal and energy efcient, allowing nations to grow fuels and
chemicals from inorganic nutrients through photosynthesis.
In recent years researchers around the world have been focus- One of the most compelling advantages for algae as a source of
ing their efforts on producing biofuels and biochemicals from the renewable energy is the potentially high rates of productivity of
growth of algae cultures. This effort has been inspired by the need many algal species compared to conventional biofuel stocks such
to develop renewable biofuels and other biochemicals in order to as corn, soybean, rapeseed, and other plants (Chisti, 2007). It has
alleviate the environmental effects associated with the use of been reported that some algae species can produce up to 30 times
petroleum fuels and chemicals, and because conventional fuel re- more bioenergy per area of land than the most productive terres-
sources are dwindling and are less secure (Li et al., 2008). In addi- trial oil seed plants (Johnson and Wen, 2010; Mata et al., 2010).
tion, algae produce some potentially high value chemicals such as This is due to rapid growth with population doubling times as
b-carotene and astaxanthin (Barowitzka, 1992). Biofuel and bio- low as 3.5 h and to algae biomass lipid/oil concentrations in the or-
chemical development from culturing microalgae simultaneously der of 1575% of the dry weight (Chisti, 2007). Algal systems also
addresses the above challenges if the technology can be economi- offer the potential to simultaneously treat wastewater and ue
gas streams by utilizing the nitrogen, phosphorus and carbon diox-
ide within these streams.
Abbreviations: EPS, extracellular polymeric substance; PBR, photobioreactor; Algal lipid yields may be enhanced by exposing cultures to a
LED, light emitting diode; BSE, backscatter electron; SE, secondary electron; FAME,
range of environmental stresses prior to harvest (Hu et al., 2008;
fatty acid methyl ester; GC, gas chromotography.
Corresponding author. Tel.: +1 (416) 9788517; fax: +1 (416) 9788605. Opute, 1974). Most of this increase in lipid concentration is in
E-mail addresses: peter.schnurr@utoronto.ca (P.J. Schnurr), george.espie@ the form of neutral lipids, particularly triacylglycerol the type
utoronto.ca (G.S. Espie), dgrant.allen@utoronto.ca (D.G. Allen). generally used for biofuel production (Hu et al., 2008), aquaculture
0960-8524/$ - see front matter 2013 Elsevier Ltd. All rights reserved.
http://dx.doi.org/10.1016/j.biortech.2013.03.036
338 P.J. Schnurr et al. / Bioresource Technology 136 (2013) 337344
feeds, and nutraceuticals (Sicko-Goad and Anderson, 1991). These tween 0.02% and 0.06% total suspended solids (TSS), and that sig-
neutral lipids form in the cytoplasm as dense lipid bodies (Hu nicant energy is spent to harvest and concentrate the cells to 5
et al., 2008; Sriharan et al., 1991), and arise from the assimilation 25% TSS. Gudin and Therpenier (1986) report that energy for har-
of new carbon (from CO2), and from the conversion of already vesting and de-watering accounts for 2030% of the total biomass
assimilated carbon in carbohydrates, proteins and other internal production costs.
biochemical molecules (Rodol et al., 2009; Roessler, 1988). To circumvent these biomass-processing costs, we have been
Although many types of environmental stress can trigger a lipid investigating the utility of growing algae as a biolm, rather than
accumulation response, the most commonly researched are nutri- in a conventional suspension culture. The idea is that by growing
ent starvation stresses. Of the many potential types of nutrient algae as a biolm the biomass can effectively be concentrated
starvation stresses, the majority of research has been done on and immobilized on a solid subsurface. Typically, biolms range
nitrogen and silicon starvation. Silicon starvation is only relevant from 616% total suspended solids (TSS) (Christenson and Sims,
for diatom algae species because it is a macronutrient essential 2011; Johnson and Wen, 2010; Ozkan et al., 2012) thereby mini-
for cell division as it composes a signicant fraction of the frustule mizing the effort required to concentrate and de-water the bio-
exoskeleton unique to diatoms (Spilling et al., 2010). Nitrogen is a mass during harvest. Additionally, the immobilized and
necessary macronutrient for all algae species because it is essential concentrated biomass can potentially reduce difculty in down-
for cell division as it composes functional groups of nucleic acids, stream processing i.e. removing substratum-biomass structure
proteins and other biomolecules (Madigan et al., 2009). and directly immersing in solvent, or removing growth medium
Although the underlying mechanism is not well understood, from the reactor and adding solvent.
researchers have clearly demonstrated that starvation of key To date, there has been very little research on biolm systems
macronutrients can signicantly increase lipid concentrations for commercial production of algae and lipids (Christenson and
within algae biomass of many different species. Opute (1974) Sims, 2012; Irving and Allen, 2011; Johnson and Wen, 2010; Ozkan
determined that the lipid concentration of Nitzschia palea could et al., 2012). Most of the algal biolm literature focuses on using
be increased from 22% to 35% after 5 days of silicon starvation. them as a means of wastewater treatment. In this paper the
Roessler (1988) reported an increase from 20% to 28% lipid concen- dynamics of a laboratory-scale algal biolm growth system were
tration when starving the marine diatom Cyclotella cryptica of sili- explored to determine algal biolm growth kinetics and lipid pro-
con for just 12 h. Sriharan et al. (1991) starved C. cryptica until lipid duction. Additionally, the hypothesis that nutrient starvation
concentrations peaked (unreported amount of time) increasing the (nitrogen and silicon) would elicit enhanced lipid production in
lipid concentrations from 21% to 42%, and from 17% to 45%, for sil- biolm cultures, as is the case in algal suspensions, was tested.
ica deciencies and nitrogen deciencies, respectively. Rodol
et al. (2009) starved a marine algae Nannochloropsis sp. of nitrogen 2. Methods
and increased the lipid concentration 4-fold (from 15% to 60%)
after 3 days of starvation. Lastly, Shifrin and Chisholm (1981) An algal biolm growth system was designed to characterize
starved 30 different algal species of nitrogen and silicon for 4 to and control many algal biolm growth parameters while maintain-
9 days and observed a 2 to 3-fold increase in lipids for green algae, ing the ability to manipulate the bulk medium nitrogen and silicon
and both increases and decreases for diatoms. Thus, nutrient star- concentrations part way through the experiment to elicit nutrient
vation can be an important industrial strategy to control and in- starvation. Additionally, the system allows for the collection of
crease the yield of neutral lipids prior to biomass harvest. growth kinetics data while doing destructive lipid analysis on the
Although there are many attractive attributes to the production biolms.
of biofuels and biochemicals from algae cultures, there are signi-
cant economical challenges to overcome before commercialization 2.1. Biolm culturing system
of these technologies can occur. One of the most signicant limita-
tions to the economical use of algae is the high cost of harvesting The semi-continuous at plate parallel horizontal photobioreac-
and concentrating the biomass (Christenson and Sims, 2011; Gudin tor (PBR) system (Fig. 1) had signicant re-circulation (95% by
and Therpenier, 1986; Uduman et al., 2010). In their review article, volume) to re-use the large amounts of medium going through
Uduman et al. (2010) state that algal suspensions are often be- the reactors. The fresh feed medium (5% by volume of total to
Fig. 1. Schematic of the algae biolm culturing system used to grow algae biolms with controls on key growth parameters.
P.J. Schnurr et al. / Bioresource Technology 136 (2013) 337344 339
each reactor) allowed for control of key nutrient (N and Si) concen- tem was intended to seed the biolm growth medium with bacte-
trations once biolms were grown and the starvation period com- ria, dissolved organic compounds, and EPS.
menced. Flow rates for the re-circulation and fresh feed streams A synthetic biolm growth medium was developed because,
within the system were controlled with Cole Parmer Masterex, compared to wastewater, it is consistent and easily characterized
Model #7523-90 peristaltic pumps. Carbon dioxide was sparged and controllable for when nutrient starvation is commenced part
into the growth medium in the mixing vessel at 2% CO2 (v/v), way through the experiments. This synthetic medium was created
which corresponds to 30 mg/L at 25 C and a total pressure of using the macro and micro nutrients of the CHU-10 diatom med-
1 atm according to Henrys Law. The concentration of CO2 was con- ium. The phosphate concentrations were amended to 75 and
trolled through the use of rotameters to control the relative ow 175 mg/L for K2HPO4 and KH2PO4, respectively, while the NH4Cl
rates of compressed air and CO2. Growth medium exited the mix- and Ca(NO3)24H2O concentrations were amended to 120 and
ing vessel into a manifold, which divided the stream into 18 sepa- 20.2 mg/L, respectively.
rate tubes. A Cole Parmer Masterex, Model 7520-50 peristaltic Immediately following the switch to biolm growth medium,
pump and 18 dampeners controlled the ow rates and hence res- the pumps were stopped in order to inoculate the PBRs. This
idence time and shear rates into 18 replicate PBRs. The shear rates was done by injecting 15 mL of an algal inoculum directly into each
used in these experiments were calculated to be 4 and 7 s 1 corre- of the 18 reactors. The inoculum was 200300 mg/L (dry weight) of
sponding to ow rates of between 13.3 and 23.3 mL/min, respec- a pure culture of N. palea (CPCC 160) or Scenedesmus obliquus (CPCC
tively, through each bioreactor. These shear rates were calculated 5) supplied by the Canadian Phycological Culture Centre, Univer-
by assuming fully developed ow, by calculating the hydraulic sity of Waterloo, ON. After inoculation, the pumps were left idle
diameter of the reactors which had rectangular cross section, the for 24 h in order to facilitate initial adhesion of algae cells to the
Reynolds Number (19 and 33 for 4 and 7 s 1, respectively), the glass substrate in the PBRs.
friction factor based on laminar ow, the wall shear stress, and The pumps then were started and the rst samples were taken
then nally, the shear rate. There were 18 (20 2 0.4 cm, for the experiment. During each experiment biolms were grown
length width height) replicate PBRs in order to facilitate for 2021 days; however, at day 1314 the nutrient starvation per-
destructive analysis with time, particularly dry weight per area of iod began by exchanging nutrient replete medium with nutrient
substrate, and lipid concentrations in the dry biomass. The PBRs depleted medium. This was accomplished in 2 ways: (1) 95% of
were illuminated with custom-made strips of 8 Watt, red-light emit- nutrient replete medium was immediately taken from system
ting diodes (LED) positioned at the top and the bottom of the PBR. and replaced with nutrient decient medium; (2) the remaining
An articial (vs natural) light system was selected to ensure that nitrogen and silicon in the lines, dampeners, reactors, etc., was di-
we had well controlled, reproducible conditions to compare the var- luted out with fresh feed of nutrient decient medium. Given that
ious biolm growth conditions. Red LED lights were chosen because the residence time in the system was about 0.11 days, virtually
chlorophyll a absorbs in the red part of the visible light spectrum complete ushing of the system was expected within 1 day (i.e. 9
and studies have shown that there are no differences in growth rates residence times).
when systems are irradiated with red light compared to all photo- The rst set of experiments was run in the biolm culturing sys-
synthetically active radiation (PAR) (Wang et al., 2007). Additionally, tem at a shear rate of 4 s 1. Under these conditions triplicate
Gordon and Polle (2007) state that on a per watt basis PAR is approx- experiments of 20 days each were run with S. obliquus as the inoc-
imately half as effective as red light in getting a photosynthetic spec- ulum. After 13 days of growth the biolms were starved of nitro-
tral response. Pond Biofuels, Toronto, Canada, supplied the lighting gen and silicon. A negative control non-starved S. obliquus culture
system. Light was maintained at 80 lmol/m2/s at both surfaces was grown for 26 days to compare to the starved cultures. The sec-
(top and bottom each) of the PBRs on a 16/8 h light/dark cycle. Light ond set of experiments was run at 7 s 1. These two separate exper-
intensities were measured as photosynthetically active radiation iments utilized two different inoculating species N. palea and S.
with a FieldScout light meter and quantum sensor from Spectrum obliquus as the inocula. Nutrient starvation commenced after
Technologies. Bulk medium temperatures were controlled at 14 days of biolm growth.
26 2 C by controlling the ambient temperature of the laboratory.
The pH was controlled at 7 0.2 with an Omega PHP-700 Series 2.2.2. Suspended cultures
pH meter and ORP Controller and Dosing Pump. Because the med- Suspension cultures of N. palea and S. obliquus were also ex-
ium drift was towards acidic conditions, a 0.5 M NaOH solution posed to nutrient decient conditions in order to determine the
was used to control the pH. Manifolds collected the PBR outlet med- enhancement of lipid accumulation, as is suggested in the litera-
ium into a single tube, which emptied into a collection ask, where- ture. This was done by growing two pure cultures of each of the
by the medium either left the system or was re-circulated. two species in asks sparged with 2% CO2 at a volumetric ow rate
of 1 L/min. Additionally, each ask was maintained at 25 C, buf-
2.2. Experimental approach fered at a pH of 6.8, and illuminated at 100 lmol/m2/s with Lumina
34 Watt cool white uorescent lights. In order to accumulate en-
2.2.1. Biolm growth ough biomass to measure lipids each culture was grown for 3
Irving and Allen (2011) determined that the presence of waste- 4 weeks with the same nutrient replete growth medium used in
water is an important factor for enhancing the formation of an al- the biolm experiments. Then, one ask was harvested the
gae biolm. The presence of bacteria and extracellular polymeric non-starved culture for lipid analysis. The second culture was
substances (EPS) in the wastewater apparently created a positive centrifuged at 4000g for 10 min to remove the nutrient replete
environment on which to seed an algal biolm. Therefore, the PBRs medium, and the cell pellet was re-suspended in the ask with
were conditioned with owing unsterile secondary wastewater nutrient decient medium void of nitrogen and silicon chemical
efuent from Ashbridges Bay Wastewater Treatment Facility, Tor- species. These cultures were then grown for another 3 days under
onto, ON, at a rate of 13.3 or 23.3 ml/min, for 48 h. It was assumed nutrient decient conditions and then harvested for lipid analysis.
that this step formed an initial biolm and EPS matrix for which
the inoculated cells could adhere. After 48 h the ow was stopped 2.3. Sampling and analysis
and approximately 90% of the wastewater was removed from the
culturing system to immediately be replaced with a biolm growth Each of the 18 replicate reactors represented a sample for anal-
medium. The 10% of residual wastewater that remained in the sys- ysis. The reactors were harvested in duplicate or triplicate, 23
340 P.J. Schnurr et al. / Bioresource Technology 136 (2013) 337344
times per week. Additionally, the sampling was designed such that used, and absorbances were measured to calculate and directly
there were 2 sets of duplicate or triplicate samples after nutrient read out the concentration of the analyte.
starvation commenced. One set was harvested and analyzed after
34 days of nutrient starvation while the other set was harvested
after 67 days. To harvest, the glass substrata were removed from 3. Results and discussion
the PBRs and scraped clean of their algal biomass. The algal bio-
mass was then lyophilized with a Labconco Lyph Lock 6 Freeze 3.1. Biolm growth kinetics and the affects of nutrient starvation
Dryer to determine dry weight. The algae biomass was then ex-
tracted to determine lipid content. In all experiments the algae biolm growth system was effec-
Biolm and suspended algae biomass neutral lipid concentra- tive in reproducibly growing thick and robust algal biolms.
tions were determined by rst extracting total lipids with the Folch Fig. 2a shows that prior to starvation the algae biolm growth
extraction method using 2:1 (v/v) chloroform to methanol as the kinetics of the three S. obliquus experiments were reproducible
solvent. Upon extraction the methanol phase of the Folch and polar and linear. Although grown in the presence of bacteria and other
lipids solution was discarded because only neutral lipids were tar- microbes, qualitative observation of the biolms shows a very
geted. Neutral lipids, predominately composed of triacylglycerides, green lm indicating a high proportion of algae (Fig. 3a). From
were targeted because they are the ones which accumulate during the date of inoculation onward the biolms gradually progressed
nutrient starvation, and because they are the preferred feedstock to being more stable and robust, having the cells rmly bonded to-
for biodiesel production (Hu et al., 2008). The chloroform was then gether as one lm. Although the biolms were primarily comprised
evaporated under a nitrogen blanket to isolate the extracted of algae, qualitative observations with SEM show signicant pres-
neutral lipids from solution. The neutral lipids were then methyl- ence of bacteria and EPS as well.
ated using the Fatty Acid Methyl Ester (FAME) technique based The algae biolm growth kinetics and biolm sloughing were
on the Microbial Identication System, Microbial ID Inc. (MIDI signicantly affected by the levels of nitrogen and silicon. As
Method) (Smid and Salnger, 1994). The samples were then ana- shown in Figs. 2a and 2b, the algal biolms grew in an approxi-
lyzed by gas chromatography (GC) using a Perkin Elmer Clarus mately linear fashion until nitrogen and silicon were ushed from
680 GC. A Hewlett Packard HP-5 MS 30 m 0.25 mm 0.25 lm the media to below their detection limit (<1 mg/L, TN; <0.01 mg/L
column was used to separate the methylated fatty acids in the
gas chromatography instrument. In order to determine the mass
of fatty acids/lipids present in the algae biomass samples, the ratio
of the area under the calibration curve to that of the internal stan-
dard was compared to those ratios in the unknowns. The calibra-
tion curve was made from 100% olive oil, while the internal
standard was hexadecane. Lastly, the lipid mass was compared to
the dry weight of algae mass to determine the percent lipid
concentration.
Scanning electron microscopy was used to observe the presence
of microbes and EPS within the biolm. Small samples of live bio-
lms were pelletized and treated for 10 min with osmium tetrox-
ide. The osmium tetroxide bonded with cellular lipids increasing
density of the high lipid biolm components, mainly algae cells.
Backscatter electron (BSE) mode was then used to observe these
osmium tetroxide treated high-density cells. Secondary electron
(SE) mode was used to image everything else present within the
eld of view i.e. bacteria, EPS, inert solids. BSE and SE images were Fig. 2a. Algae biolm growth kinetics grown at a shear rate of 4 s 1. Triplicate
experiments were run to determine the affects of nutrient starvation on growth
overlayed upon each other in order to show the high resolution al-
kinetics. Nutrient starvation of N and Si began at the 13th day of growth. A fourth
gae cells within the biolm matrix. The scanning electron micro- experiment was run for 26 days without any nutrient deciencies.
scope used was a Hitachi S-3400N.
Total nitrogen and total silicon analysis was conducted on the
bulk medium to measure the level of these nutrients prior to star-
vation, and to follow the disappearance of these nutrients after the
starvation period commenced. These analyses were conducted
using HACH chemical reagent kits, which utilizes principles of col-
orimetric analysis. The chemical reagent kits used for total nitro-
gen were TNTplus (827) HR for high range analysis of 540 mg/L,
and TNTplus (826) LR for low range analysis of 116 mg/L. For
determination of total silicon the reagent kit Silica Reagent Set
High Range (1100 mg/L), and the Silica Reagent Set Low Range
(0.011.60 mg/L) were used.
For preparation and analysis of the samples a HACH digestion
instrument and a HACH spectrophotometer were used. The HACH
digestion (DRB200) unit was used to digest all nitrogen compounds
into a single form that would react with the HACH reagent sup-
plied in the kit. Upon digestion and treatment of samples with
the HACH reagent, a HACH DR3900 spectrophotometer was used
to analyze all samples for total nitrogen and total silicon concen- Fig. 2b. Algae biolm growth kinetics grown at a shear rate of 7 s 1 for both
trations. The wavelengths for analysis were programed directly Nitzschia palea and Scenedesmus obliquus. Nutrient starvation at the 14th day of
into the spectrophotometer according to the reagent kit being growth.
P.J. Schnurr et al. / Bioresource Technology 136 (2013) 337344 341
Fig. 3a. Algae biolms inoculated with S. obliquus and grown for 14 days under nutrient replete conditions.
Fig. 3b. Algae biolms inoculated with S. obliquus and grown for 14 days under nutrient replete conditions followed by 3 days of exposure to nutrient decient conditions.
Si), after which point the biolms signicantly sloughed from the or silicon limitations within algal-bacterial biolm consortia, a
substratum it was xed to (Fig. 3b), and/or ceased to grow. Lag similar phenomenon of microbe nutrient acquisition may be occur-
phases and sloughing events were variable between the triplicate ring in these consortiums.
S. obliquus experiments ran at 4 s 1; these types of variability are In addition, quorum-sensing mechanisms within biolms could
not uncommon in biological systems. A non-starved culture of S. lead to other reasons for dis-attachment within the lm (Decho,
obliquus grew for 26 days without any cessation of growth or sig- 1999). Wingender et al. (1999) suggest that cell-to-cell communi-
nicant sloughing. The N. palea biolm grown at 7 s 1 had the least cations are fundamental for biolms to adapt and respond to cer-
amount of observable and quantiable sloughing. tain environmental conditions, including nutrient limitations. It
The cessation of growth with nitrogen limitations is expected may be that the cells exposed to adverse conditions such as nutri-
because without this nutrient these organisms cannot produce ent limitations will utilize an adaptive strategy whereby they deat-
the amino acids, nucleic acids, and proteins necessary for growth tach from the substratum in order to reinhabit a new environment
and reproduction (Madigan et al., 2009). Similarly, diatoms cannot where nutrients are more abundant.
produce the frustule exoskeleton without silicon (Spilling et al., Linear, rather than exponential growth, suggests some chemical
2010). Opute (1974) suggested another potential reason for growth or physical limitations to growth. This could be due to limitations
inhibition. He suggested that, when starved of key nutrients, algae of light and nutrient transport, and/or the result of cell deattach-
release an autotoxin that inhibits cell division while photosynthe- ment from the biolm. The linear growth could also be a result
sis continues to assimilate carbon and store it in the form of lipids. of allocation of electrons and other resources i.e. carbon, nitrogen,
Since growth is inhibited during nutrient starvation the overall va- into building the EPS matrix rather than reproduction (Laspidou
lue of using this method to increase lipid productivities is debat- and Rittmann, 2002). Wolfaardt et al. (1999) suggest that in bacte-
able because the growth rate-lipid concentration trade-off is rial systems up to 70% of the carbon and energy can go into the
delicate (Opute, 1974; Rodol et al., 2009). production of EPS, rather than cell reproduction. Additionally, Da-
Sloughing of the biolms may be a result of EPS matrix degrada- vies (1999) determined that when bacterial cells form biolms
tion by enzyme release induced by nutrient starvation. (Davies, they make up to 19 times more EPS than planktonic cells. Algae
1999; Wingender et al., 1999). Davies (1999) suggests that chang- cells may behave similarly in the sessile state.
ing environments will cause bacterial cells to down regulate algi- The differences in sloughing observed between N. palea and S.
nate promoters causing much less alginate to be produced, obliquus (Fig. 2b) might be due to the biological properties of the
eventually leading to cell dettachment and sloughing. Additionally, two species N. palea is a diatom and diatoms are generally more
and perhaps more importantly, Davies (1999) states that when ex- of a sessile group of algae species whereas green algae (S. obliquus)
posed to vital nutrient deciencies, bacteria can release alginate are more of a planktonic-type group of species (Congestri et al.,
lyase, leading to the degradation of the EPS matrix bonding the al- 2006). Additionally, through observations in the lab, and corre-
gae cells to each other and to the substratum. Recall that the liter- spondence with the Canadian Phycological Culture Centre, N. palea
ature suggests, and that the research in this paper anticipated, was determined to be a very adherent species, readily forming bio-
biolms bound together by EPS produced by bacteria; however, al- lms and ocs under sterile environments. Thus, N. palea is likely
gae cells may also behave in a similar fashion as bacteria when ex- to form a more robust and resilient biolm, even while exposed
posed to nutrient decient conditions i.e. down regulate alginate to nutrient decient conditions for relatively short periods of time.
promoters and release enzymes.
Hydrolysis and degradation of the EPS may also occur during 3.2. Lipid concentrations of algae grown in nutrient replete and
periods of nutrient starvation in an attempt to either utilize the decient conditions
nutrients within the biomolecules in the matrix (Wingender
et al., 1999), or to dis-attach and re-populate where more nutrient In contrast to planktonic algae, algal biolms did not accumu-
favorable conditions exist. Wingender et al. (1999) and Wolfaardt late more neutral lipids when exposed to nutrient decient condi-
et al. (1999) both suggest that bacteria will use enzyme-substrate tions for 34 or 67 days (Fig. 4). There was no signicant
interactions in order to utilize nutrients in EPS during periods of difference between the neutral lipid concentrations of either
nutrient stress. Although these authors mostly write about carbon S. obliquus or N. palea after 3 or 6 days of starvation. On the
nutrient limitations on bacterial biolms, and not about nitrogen other hand, consistent with the literature, planktonic N. palea and
342 P.J. Schnurr et al. / Bioresource Technology 136 (2013) 337344
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