FAMILY ENTEROBACTERIACEAE - eosin Y & methylene blue as gram (+)

bacteriostatic agents
- gram negative - lactose as CHO source
- motile except SKY - eosin Y & methylene blue as pH indicator
- facultative anaerobe - (+) result: pink to red or black w/
- ubiquitous organisms
greenish metallic sheen
- Nitrate reduction (+)
- E. coli produces black greenish metallic
- Oxidase (-)
sheen
- glucose fermenter
SSA
Isolation Media
- bile salts as gram (+) bacteriostatic agent
- majority of Enterobacteriaceae are
- lactose as CHO source
present in stool - neutral red as pH indicator
- tissue specimen & biopsy is also used
- detects H2S production
Enrichment Medium - (+) result: pink to red colonies
- (-) result: colorless
- lets all microbes live except for normal
Xylose Lysine Desoxycholate (XLD)
microflora
- includes Selenite, Gram negative & - bile salts as gram (+) bacteriostatic agent
Tetrathionate broth - xylose, lactose & sucrose as CHO source
- phenol red as pH indicator
Non-Selective Medium - detects H2S production
- non-inhibitory - (+) result: yellow colonies
- (-) result: red colonies
- lets all microbe live
- forms grayish-colonies Hektoen Agar
- includes BAM
- bile salts as gram (+) bacteriostatic agent
Selective Medium - salicin, lactose & sucrose as CHO source
- bromthymol blue as pH indicator
- allow some microbe to live
- depends on selectivity - detects H2S production
- (+) result: yellow, orange or salmon pink
Slightly Selective colonies
- (-) result: green or blue-green colonies
- includes MAC & EMB
- differentiates lactose fermenters from Bismuth Sulfite Agar
non-lactose fermenters
- bismuth & brilliant green as gram (+)
Moderately Selective bacteriostatic agent
- glucose as CHO source
- includes SSA, Xylose Lysine Agar & - bismuth sulfite as pH indicator
Hektoen Agar - (+) result: green colonies
- detects H2S production - (-) result: colorless
- forms black colonies
Bismuth Green Agar
Highly Selective
- brilliant green as gram (+) bacteriostatic
- includes Bismuth Sulfite & Brilliant Green agent
Agar - lactose as CHO source
- for Shigella species - phenol red as pH indicator
- detects H2S production
Enteric Selective & Differential Media - (+) result: yellow to green colonies
- (-) result: white to red colonies
MAC

- crystal violet & bile salts as gram (+)

bacteriostatic agents
- lactose as CHO source
- neutral red as pH indicator
- (+) result: pink to red colonies
- (-) result: colorless

EMB