Professional Documents
Culture Documents
DIAN PUSPITAWARNI
1508062235
Supervisor
DEPARTMENT OF APOTHECARY
FACULTY OF PHARMACY
AHMAD DAHLAN UNIVERSITY
YOGYAKARTA
2016
r' -!-l
:
APPROVAL PAGE
SYSTEMATIC REVIEW
15a8a62235
Author,
Confirmed by,
COVER........................................................................................................................... i
APPROVAL PAGE ........................................................................................................ ii
TABLE OF CONTENTS ............................................................................................... iii
LIST OF TABLE ............................................................................................................ iv
LIST OF FIGURE .......................................................................................................... v
ABSTRACT ................................................................................................................... vi
A. BACKGROUND ....................................................................................................... 1
B. METHOD .................................................................................................................. 2
1. Searching Method ............................................................................................... 2
2. Reference Selection Method ............................................................................... 2
3. Data Extraction ................................................................................................... 3
4. Processing and Data Assessment ........................................................................ 3
C. RESULT .................................................................................................................... 4
D. DISCUSSION ............................................................................................................ 4
E. CONCLUSION .......................................................................................................... 6
F. REFERENCE............................................................................................................. 6
iii ii
LIST OF TABLE
iv
iii
LIST OF FIGURE
Viv
ANTIBACTERIAL ACTIVITY OF Jatropha curcas L.
ROOTS METHANOL EXTRACT AGAINST
Staphylcoccus aureus
Dian Puspitawarni, Muhammad Muhlis
Faculty of Pharmacy, Ahmad Dahlan University
Correspondence :
dianpuspita36@gmail.com
Keyword: Antibacterial, Jatropha curcas L., root, methanol extract, Staphylococcus aureus
v
vi
A. Background
In the part few years, there has been an increasing interest in use of natural
bioactive compound for medicine. The plant is an important source of natural
bioactive compounds. According to WHO, about 80 % of people in worldwide still
rely on herbal medicines. There are thousands of important medicinal plants which are
medicinally important and also
recommended for to cure various infectious
disease that cause by bacteria and yeast.
One of bacteria that can cause infection is
Staphylococcus aureus. Staphylococcus
aureus is gram positive bacteria, spherical
shape with diameter 0,7 1,2 m,
facultative anaerobic, unforming spore,
Figure I. Staphylococcus aureus
and unmoved (Figure I). This bacteria
grows at optimum temperature 37C, but form pigments the best temperature in room
temperature (20-25C) (Jawetz et al., 1995; Novick et al., 2000). Microorganisms
have developed resistance to many antibiotics because of inaccuracies using
commercial antimicrobial, so scientist are encouraged to finding new antimicrobial
from variety source including medicinal plants (Karaman et al., 2003). Medicinal
plants are one of important source of antimicrobial agent. Antimicrobial is substance
that can kill/ inhibit the growth of pathogenic microorganisms.
One of the plants that have been used in society is Jatropha curcas L. Jatropha
curcas belongs to the family Euphorbiaceae, Jatropha genus (Backer and Brink,
1965). Jatropha curcas L. is a plant that
has been cultivated for a long time ago in
worldwide. Natural compound of Jatropha
shows bioactive activity for fever, mouth
infection, jaundice, and rheumatic joints
(Aiyelaagbe et al., 2007). Extracts from
various parts of Jatropha curcas such as
seeds, seed oil, stem barks, roots and leaves Figure II. Jatropha curcas Linn.
have shown fungicidal (Nwosu and Okafor,
1995; Saetae and Worapot, 2010) and bactericidal properties (Igbinosa et al., 2009).
1
Alkaloids, saponins, tannins, anthraquinones and flavonoids of active compounds are
known to have activity against several pathogens (Hassan, et al., 2004).
The hypothesis in this review is methanol extract of Jatropha curcas L. root has
antibacterial activity against Staphylococcus aureus. Based on the hypothesis, so the
objective of this review aimed to determine antibacterial activity methanol extract of
Jatropha curcas L. root against Staphylococcus aureus based on research that has
been published.
B. Method
This study used Systematic Review method with descriptive design. This study
is made in March until May 2016.
1. Searching Method
a. The strategy of article searching is based on PICO analysis.
Table I. PICO Assessment
PICO Data
Problem Antibacterial
Comparation Placebo
2
5) Using methanol extract of Jatropha curcas L. root
6) Studied use Staphylococcus aureus bacteria
b. Exclusion Criteria:
1) Incomplete publication
2) Not as an antibacterial effect
3) There is no MIC values and clear zone
3. Data Extraction
Result of data extraction from reference obtained as follows (Table II).
Table II. Search Result Data
Number of Article
Reference Search Result
Received
Google 5440 3
www.scholar.google.com 325 2
From some result of the research was found, then obtained some article selected
that are (Table III).
Table III. Result of Article Selected
Tittle, Author, Year
3
C. Result
D. Discussion
4
Table V. Result Data Review Article
Minimum Inhibition Concentration Minimum Inhibitory Zone
Article
(MIC) (Clear Zone)
1 2,3 mg/ml 14 mm
5
E. Conclusion
F. Reference
Aiyelaagbe, O.O., Adeniyi, B.A., Fatunsin, O.F. and Arimah, B.D. (2007). In-vitro
antimicrobial activity and photochemical analysis of Jatropha curcas roots.
Intern. J. Pharmacol., 3(1): 106-110.
Anwar, S., Muhamad, Z., Rashis, U., et. al. (2013). Antimicrobial Potential of
Methanolic Extract and Various Fractions of Jatropha curcas Roots. Asian
Journal of Chemistry. Volume 25: 7477-7480.
Backer, A and Van Den Brink, B. (1965, Flora of Java (Spermatophytes Only).
Volume I. N.V.P. The Nederlands. Noordhoff-Groningen.
Hassan MM, Oyewale AO, Amupitan JO, Abduallahi MS, Okonkwo EM. Preliminary
phytochemical and antibacterial investigation of crude extracts of the root bark
of Detarium microcarpum, J. Chem. Soc. Nigeria. 2004; 29:26-29
Igbinosa, O.O., Igbinosa, E.O. and Aiyegoro, O.A. (2009). Antimicrobial activity and
phytochemical screening of stem bark extracts from Jatropha curcas (Linn).
African Journal of Pharmacy and Pharmacology, 3(2): 58-62.
Jawetz, E., J.L. Melnick., E.A. Adelberg., G.F. Brooks., J.S. Butel., dan L.N. Ornston.
1995. Mikrobiologi Kedokteran. Edisi ke-20 (Alih bahasa : Nugroho &
R.F.Maulany). Jakarta : Penerbit Buku Kedokteran EGC. hal. 211,213,215.
Karaman, I., Sahin, P., Gulluce, M., Oguten, H., Songul, M. and Adiguzed, A. (2003).
Antimicrobial activity of aqueous and methanol extracts of Juniperus oxycedrus
L. J Ethanopharmacol., 2837: 1-5.
Naidu, A. S. 2000. Natural Food Antimicrobial System. CRC Press, USA.
Namuli A, Abdullah N, Sieo CC, Zuhainis SW, Oskoueian E. (2011). Phytochemical
compounds and antibacterial activity of J. curcas L. extracts. J. Med. Plants
Res., 5: 3982-3990.
Nwosu, M.O. and Okafor, J.I. (1995). Preliminary studies of the antifungal activities
of some medicinal plants against Basidiobolus and some other pathogenic fungi.
Mycoses, 38: 191-195.
Obasi, N.L., Madus, P., Ejikeme., Cemaluk A.C., Egbuonu. 2011. Antimicrobial and
phytochemical activity of methanolic extract and its fractions of Jatropha curcas
Linn (Eurphorbiaceae) stem bark. African Journal of Pure and Applied
Chemistry. 5(5) : 92-96. ISSN 1996 0840.
Saetae, D. and Worapot, S. (2010). Antifungal Activities of Ethanolic Extract from
Jatropha curcas Seed Cake. J. Microbiol. Biotechnol., 20 (2): 319-324.
Sharma, A.K., Gangwar, M., Tilak, R., et. al. 2012. Comparative In Vitro Antimicrobial
and Phytochemical Evaluation Methanolic Extract of Root, Stem and Leaf of Jatropha
curcas Linn. Pharmacognosy Journal. Volume 4. Issue 30.
Vaquero MJR, Alberto MR, Nadra MCM (2007). Antibacterial effect of phenolic
compounds from different wines. Food Control, 18: 93-101
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WHO. 2002. Traditional medicine growing needs and potential. WHO policy
perspective on medicines. World Health Organization. Geneva. 1-6.