5/14/2015 JournalofAccordIntegrativeMedicine

JournalofAccordIntegrativeMedicine
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2009,Volume5,Number1

ISSN19324642

ImpactofanexternalenergyonEnterococcusfaecalis
[ATCC51299]inrelationtoantibioticsusceptibility
andbiochemicalreactionsAnexperimentalstudy
2009,Volume5,Number2:119130

MahendrakumarTrivedi(FounderPresident1,2,FounderChairman3),Dr.Yogi

Bhardwaj(President3),

ShrikantPatil(Associate)1*,HarishShettigar(Trustee2),ArchanaBulbule(Treasurer2)

1SocietyforDivineLife,A14,KanwalApartment,FourBungalows,Andheri(W.),

Mumbai,MaharashtraINDIA

2AdvancedSocietyForDivineLife,Bhopal(MadhyaPradesh)INDIA

3DivineLifeFoundation,1680,N.DelanyRoad,Gurnee,Illinois600311238USA

*CorrespondingauthorShrikantPatil

Emailaddressthelifeenergy@gmail.com
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Contactnumber+1(847)8760898

Categoryofsubmission:InVitroStudy

Institutioninwhichtheworkwasperformed:

MicrobiologyLaboratoryofP.D.HindujaNationalHospital&MedicalResearchCentre,
Mumbai,IndiaaccreditedbyTheCollegeofAmericanPathologists

KeywordsAntibioticresistance,BiochemicalReactions,Lyophilization,
Polymorphism,Energy,Model

Abstract

Background:

Whilespiritualandmentalenergiesareknowntoman,theirimpacthasneverbeen
scientificallymeasurableinthematerialworldandtheyremainoutsidethedomainof
science.ThepresentexperimentsonEnterococcusfaecalis[ATCC51299],reportthe
effectsofsuchenergytransmittedthroughaperson,Mr.MahendrakumarTrivedi,which
hasproducedanimpactmeasurableinscientificallyrigorousmanner.

Methods:

Enterococcusfaecalisstrainsinrevivedandlyophilizedstateweresubjectedtospiritual
energytransmittedthroughthoughtinterventionand/orphysicaltouchofMr.Trivedito
thesealedtubescontainingstrain,theprocesstakingabout3minutesandwereanalyzed

within10daysafterincubation.Alltestswereperformedwiththehelpofautomationon
theMicroscanWalkawaySysteminMicrobiologyLaboratoryaccreditedbyThe
CollegeofAmericanPathologists

Results:

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TheresultsindicatedthatMr.Trivedisenergyhaschanged9of27biochemical
characteristicsofEnterococcusfaecalisalongwithsignificantchangesinsusceptibility
patternin5of31antibiotics.TheBiotypenumberhaschangedfromtheoriginalcontrol

straingivingriseto2differentbiotypesintreatedsampleswhiletheexternalenergy
/treatmentgivenwasthesameforalltreatedsamplessuggestiveofrandom

polymorphismasanalyzedthroughtheautomatedmachine.

Conclusions:

Theseresultscannotbeexplainedbycurrenttheoriesofscience,andindicateapotency
inMr.Trivedisenergy,providingamodelforsciencetobeabletoinvestigatethe

impactofspiritualenergyinarigorousmanner.

Inlyophilizedstate,biochemicalandenzymaticcharacteristicscouldbealtered.

Introduction

Alllivingorganismscontainlargecomplexmoleculeswithinthecells.Someoftheseare
proteinsandenzymesincludingDNA/RNA.Thenatureoftheorganismsisdependent
onthebiochemicalreactions,whichcanbecharacterizedandidentified.

Antibioticresistance,regardlessofantibioticandbacteria,willoccurwithsufficienttime

anddruguse.Widespreadantibioticusecausesselectionpressure:resistantstrains
survivewhilesusceptibleonesareeliminated.Antibioticresistanceisprogressive,
increasingfromlowtointermediatetohighlevels[1].Bacterialstrainsarealso
characterizedbytheirrelativeresistancestoarangeofantibiotics.However,sucha
changedoesnottakeplacespontaneouslyinastrainovera10dayperiodofnormal
incubationwithoutcontinuousexposuretoanydrugs.

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InthispaperwereporttheimpactofspiritualenergyonEnterococcusfaecalis,in
revivedandlyophilizedstate,withrespecttoitsantibioticsusceptibilitypatternalong

withbiochemicalpropertiesanalyzedwithinaperiodof10days.Thesaidenergywas
transmittedthroughthoughtinterventionofanindividual,Mr.MahendrakumarTrivedi
whohasbeeninteractingwithalargenumberofpeopleasahealeroverthelastdecade.
Asresponsesbyhumanscanbeaccountedforbytheplaceboeffect,theseexperiments
onlowerorganismsweredesignedinordertodirectlytesttheimpactthroughscientific
studiestoruleouttheplaceboeffect.

Itiswidelyacceptedthatlyophilizationisthemethodmostcommonlyusedtostoreand
transportmicrobialculturesaschangeinthebiochemicalandenzymaticcharacteristics

ofanorganismcannotbecarriedoutinthisstate.

MaterialandMethods

TwostrainsofEnterococcusfaecalis[ATCC51299]wereprocuredfrom
MicroBioLogicsinsealedpacksbearingthesameATCCnumberandstoredaccording
totherecommendedstorageprotocolsuntilneededforexperiments.Thestudywas
groupedasperthefollowing.

GroupI:

OneofthetwosealedpacketswashandedovertoHindujaMicrobiologyLabandwas

revivedbythemintwoseparatetubes,ofwhichonewasthecontrol.Thecontroltube
wasanalyzedforidentification,antibioticsusceptibilityandbiochemicalreactionsasper

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thestandardprotocolsofsampleprocessinginthemicrobiologylab.

Thesecondtube,(ATCCA)havingviablebacterialculture,washandedoverto

Mr.Trivedifortreatment,aftersealingbyparafilm.Itwasassessedonthe5thand10th
daysaftertreatment.

Treatment:

Mr.Trivediheldthistubeinhishandunderambientconditionsforbetween0.5to3

minuteswhiletreatingitthroughhisthoughtinterventionprocessbycommunicatingand
instructingtheexperimentalobjectwithinthetubeinordertoundergothechange.The
tubewasreturnedtothelabinthesealedconditionitself.

GroupII:

ThesecondsealedpacketofEnterococcusfaecalis[ATCC51299](ATCCB)was
treatedbyMr.Trivedidirectlyinthesealedlyophilizedstate,usingthesametreatment

processasabove.Thesealedtubeswerebrokenandthestrainswererevivedand

analyzedonthe10thdayforidentification,susceptibilitytestingandbiochemical
reactions.

AlltestswereperformedwiththehelpofautomationontheMicroscanWalkaway
System(DadeBehringSiemens)usingPBPC20panels.Antimicrobialsusceptibility
wasdeterminedusingtheMinimumInhibitoryConcentration(MIC)methodasperthe
latestCLSIguidelines.

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MicroScan

Afterinoculationandrehydrationwithastandardizedsuspensionoforganismand

incubationat350cfor16hrs,theminimuminhibitoryconcentration(MIC)orthe
qualitativesusceptibility(susceptible,intermediateorresistant)wasdeterminedby
observingthelowestantimicrobialconcentrationshowinginhibitionofgrowth.The
resultsofsusceptibilitytestingwereexpressedinmillimetersofgrowthinhibitionwith

disktestingandinmcg/mlinMICtesting.

Qualitycontrol:

Theacceptabilityoftheidentificationmediaandantimicrobialagentswascheckedprior
tothestudybyATCCcontrolorganisms,SAureusATCC29213,&EColiATCC
25922.

Panelused:PBPC20

ListofAntibioticstested:

Amoxycillin/kclavulanicacid,Ampicillin/Sulbactam,Ampicillin,Cafazolin,

Cefepime,Cefotaxime,Ceftriaxone,Cephalothin,Chloramphenicol,Ciprofloxacin,
Clindamycin,Erythromycin,Gatifloxacin,Gent.Synergy,Gentamicin,Imipenem,

Levofloxacin,Linezolid,Moxifloxacin,Nitrofurantoin,Norfloxacin,Ofloxacin,
Oxacillin,Penicillin,Pip/Tazo,Rifampin,Strep.Synergy,Synercid,Tetracycline,

Trimethoprim/SulfaandVancomycin.

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FollowingBiochemicaltestswereperformed:

Urea,Lactose,Arginine,CrystalViolet,Novobiocin,IndoxylPhosphatase,Voges

Proskauer,Nitrate,Pyruvate,Glycosidases,Arabinose,Raffinose,Sorbitol,RBS,PGR,
MS,OPT,PHO,BE,PYR,MAN,TRE,MNS,BAC,HEM,INUandNACL.

Setting:

MicrobiologyLaboratoryofP.D.HindujaNationalHospital&MedicalResearchCentre,

Mumbai,IndiaaccreditedbyTheCollegeofAmericanPathologists

Results:

Inthispaper,onlythechanges/variationsinantibioticsusceptibilityandbiochemical
reactionsthatwereobservedbeforethetreatment(control)andafterthetreatment

(treated)havebeenreportedinatabularformattachedherewith.

Theresultsoftheantibioticsusceptibilitytests,identificationtestsandbiochemicaltests

canbeseeninTable1.

DetailsofMICvalueshavebeenpresentedinTable2.

AntibioticSusceptibility

GroupI:

Forfourofthefiveantibioticsshownhere,Ampicillin,Linezolid,Penicillin,

Tetracycline,theMICvaluesintheTreated5thDayremainedunalteredasincontrol,

andfurtherincreasedintheTreated10thDaychangingthesusceptibilityfromsensitive

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inboththecontrolandTreated5thDaysamplestoaresistantstrainbythe10thday.

ForVancomycintheMICvaluebeing16inthecontrol,hadremainedthesamebyDay

5,andincreasedto>16byDay10,changingthesusceptibilityfromintermediateasin

controlandtreated5thdaytoresistantbythe10thday.

GroupII:

InGroupIIsamplestoo,althoughtreateddirectlyinthelyophilizedform,achangemay

benotedinthesusceptibilitytoVancomycin,whoseMICvaluesdecreasedafter
treatmentfrom16inthecontrolto4inthetreatedsample,changingthesusceptibility

fromintermediateinthecontroltosensitiveinthetreatedsamplebyDay10.Whereas
thesusceptibilitytoAmpicillin,Linezolid,PenicillinandTetracyclineremained

unalteredbeingsensitiveasincontrol.

OrganismIdentification

Biotypenumbersofparticularorganismswerearrivedatafterinterpretingtheresultsof
thebiochemicaltests.TheBiotypenumbersthenledtotheparticularOrganism

Identification.

Surprisingly,thebiotypeshavechangedinGroupIandGroupIIsamplesbythe10thday
comparedtocontrolanddidnotmatchwitheachother.

BiochemicalReactions

GroupI:

InGroupI,forVogesProskauer,Lactose,RBSandPyruvate,thereactionswere
positiveinthecontrolsamplesandhadremainedthesamebyDay5followedby
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changingtonegativeonthe10thday.

IncaseofNitrate,IndoxylPhosphatase,BEandUrea,thereactionswerenegativeinthe
controlsamplesandhadremainedthesamebyDay5followedbychangingtopositive

onthe10thday.

InthecaseofHEM,nochangeobservedaftertreatment.

GroupII:

InthreeofthebiochemicaltestschangesmaybenotedintheGroupIIsample,which

wastreateddirectlyinthelyophilizedform.ThereactionwithNitrate,Indoxyl
PhosphataseandHEMhaschangedfromanegativeresultinthecontroltopositiveby

thetimeitwastestedonthe10thday.Restofthebiochemicalsremainedthesameasin
control.

Discussion:

Whilemisidentificationsanderrorsinassessmentoftheabovecharacteristicsareknown

tohaveoccurredinsuchtests,theexistenceofchangesinsomanypropertiesandtests
cannotbedismissedasamachineerror.Thefindingsofalterationsinantibiotic

susceptibilitypatternsaswellasinseveralbiochemicalreactionsasaresultofthe
treatmentappliedareunexpectedandunprecedentedbasedontheliteraturesofar,and

currentlyunexplainedbyscience.Thebiotypesoftheorganismshavealsochangedafter
treatmentbyMr.Trivedi.Thisappearstoindicatethepresenceofanenergytowhichthe

organismswereexposed.Andmostimportantisthattheparticularmicrobereactedto

thisenergy[2,3].

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Inseparatestudies,theeffectofMr.TrivedisenergyonbacterialstrainsofATCC
throughDNAfingerprintinghasindicatedDNApolymorphismrangingfrom4%to79%

[4](unpublished).Further,16SrDNAtestsshowachangeinthebacterialspecies.[5]
(unpublished).Suchobservationsareindicativeofalterationsingenotype.The

similarityofeffectofMr.Trivedisuniqueenergyonbiochemicalchangesand

susceptibilitypatternsofEnterococcusfaecalis[ATCC51299]andotherbacteria
referencedabove[4,5]leadustopostulatethatEnterococcusfaecalis[ATCC51299]

hasalsogonethroughagenotypechange.ThusweexpecttoseeDNApolymorphismin
thesestrains,whichmayberesponsibletocausesuchchangesinantibioticsusceptibility

aswellasinbiochemicalreactions.

Theresultsobtainedinthepresentexperimentindicatetheexistenceofanenergythatis

stillnotknowntocontemporaryphysicalandlifesciencesandwhichcaneffectively

alterthecharacteristicsofthemicroorganismssuchasthebacteriastudiedinthiswork.
Itisnotpossibletofindanexplanationofsuchobservationsbasedonthecurrent

scientificknowledge.Theseresultsmaygiverisetobetterinsightintothepathwaysthat
leadtomutationandmayalsohelptounderstandthepossiblebiochemicalpathwaysthat

takeplaceinthelifecycleoftheparticularmicrobe.

Whilesciencehassofarremainedunabletoenterintoquestionsregarding

consciousness/Spiritualismandorderedforcesorenergieswhichcaninterconnect
materialsonaplaneofhigherinformation,theresultsofthecurrentexperimentsprove

theexistenceofadirectmodelforinvestigationintotheimpactofaspiritualenergyina

rigorousmanner.

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TheimpactofMr.Trivedisenergyhasalsobeentestedonvarioustransitionmetals

resultingintochangeinatomicandcrystalparameters.Thetreatedmetalpowders
exhibitedincreaseaswelldecreaseinvolumeofunitcell,effectivenuclearchargeper

unitvolumeoftheatomandtheatomicweight.Thechangesinatomicparametersare
significantenoughtoincreaseanddecreasethecrystalliteaswellasparticlesizes.[6]

Thermalanalysisofthetreatedmetalpowdersalsoshowedadecreaseinlatentheatof
fusiontherebyindicatingthetreatedpowderstobeinahighenergystate.[7]

Theresultsoftheexperimentsoninorganicmaterialsshowthatthechangesareatthe
atomiclevels.Asthebuildingblocksoflivingorganismscontainmanytypesof

chemicalelements,theenergyhasalsobeenabletoaffecttheassembliesofatomsand
thechemicalbondsinthiscase.

Conclusions:

1Mr.Trivedisenergyhasalteredthebiochemicalreactionsalongwiththeantibiotic

susceptibilityofEnterococcusfaecalisonlywithintheperiodof10days.

2.Thechangesoccurredinthreebiochemicalreactionsinthelyophilizedstateare

impossibleasperthepresentknowledgeandwouldbeconsideredmiraculous.

3.Lyophilizationisthemethodmostcommonlyusedforthepreservationof

microorganisms,asitdoesnotaltertheChemicalandenzymaticcharacteristicsofthe
microbes.Oncontrary,thepresentedresultshaveobservedthechangesinfew

characteristicsofEnterococcusfaecalisinthelyophilizedstateitselfindicatingtheneed
forsuperiormethodforstorageofparticularmicroorganism.
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4.Theseresultscannotbeexplainedbycurrenttheoriesofscience,andindicatea
potencyinMr.Trivedisenergy,providingamodelforsciencetobeabletoinvestigate

theimpactofspiritualenergyinarigorousmanner.

5.Inlyophilizedstate,biochemicalandenzymaticcharacteristicscouldbealtered.

Acknowledgements

TheauthorswouldliketoacknowledgeMs.NandiniAltekarforhelpingusinwritingthe

paper.

References:

1. ChangingAntibioticSusceptibilityPatternsataUniversityHospital[SouthMedJ
94(6):619620,2001.2001SouthernMedicalAssociation]
2. MahendraKuamrTrivedi&S.Patil,Impactofanexternalenergyon
Staphylococcusepidermis[ATCC13518]inrelationtoantibioticsusceptibility
andbiochemicalreactionsAnexperimentalstudy,JournalofAccordIntegrative
Medicine,Nov.2008,Volume4,Number4.
3. MahendraKuamrTrivedi&S.Patil:ImpactofanexternalenergyonYersinia
enterocolitica[ATCC23715]inrelationtoantibioticsusceptibilityand
biochemicalreactions:Anexperimentalstudy.TheInternetJournalofAlternative
Medicine.2009Volume6Number2
4. http://www.divinelife.us/Genetics/staphylococcus_aureus.htm
5. http://www.divinelife.us/Genetics/B_Nocardia_otitidis_Treated_A.htm
6. MahendraKumarTrivedi&RamaMohanTallapragada,Atranscendentalto
changingmetalpowdercharacteristics,MetalPowderReport,Volume63,Issue9,
October2008,Pages2228,31.
7. VikramV.Dabhade,RamaMohan.T.R,MahendraKumarTrivedi,Effectof
externalenergyontheatomic,crystalline,andpowdercharacteristicsofantimony
andbismuthpowders,BulletinofMaterialsScience(AcceptedForPublication)

Table1:Analysisofbiochemicalreactions,Biotype,OrganismIdentificationNameand

Antibioticsusceptibilitypatternsofallsamples.

GROUPI GROUPII
Name Antibiotics& Control Treated Treated Lyophilized

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ofthe Biochemical 5thday 10thday Treated

bacteria Reactions Directly,
& 10thday
ATCC
no.
ATCCB

ATCC ATCCA
A
E.
faecalis
ATCC Ampicillin S S R S
51299
Linezolid S S R S
Penicillin S S R S
Tetracycline S S R S
Vancomycin I I R S

Biotype 6473 6473 753773604 757357647
57646 57646
Organism

Identification E. E. E.faecalis E.faecalis

faecalis faecalis
Name

VogesProskauer + + +
Lactose + + +
Rbs + + +
Pyruvate + + +
Nitrate + +
IndoxylPhosphatase + +
Hem +
Be +
Urea +

Key:S=susceptible,I=intermediate,R=resistant,+=positive,=negative

Legend:

ControlreferstoATCCstrainsthatwereanalyzedbeforethetreatment.

TreatedreferstoATCC'A'on5th&10thdayafterthetreatment&
ATCC'B'lyophilizedthatweretreateddirectly

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Table2:MinimumInhibitoryConcentrationvaluesofantibioticsinmcg/ml.

GROUPI GROUPII
Antibiotics Control Treated Treated Lyophilized
10thday Treated
5thday Directly



ATCCA ATCCB
ATCCA

Ampicillin 2 2 >8 2
Linezolid <=2 <=2 >4 <=2
Penicillin 2 2 >8 2
Tetracycline <=4 <=4 >8 <=4
Vancomycin 16 16 >16 4

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