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1-chromosomes
2-plasmids
Enzymes which slice DNA into fragments at specific
sequences (4-8 base pairs long)
When sliced at the ends, the DNA becomes sticky if there is
a single-strand exposed (overhang)
Certain R E need to be used to ensure the gene being analyzed
is removed completely from the DNA
Multiple R E are being used at the same time
Also called restriction enzymes
Example: EcoR1
Genus: Escherichia
Species: coli
Strain: R
Order discovered: 1
Enzymes recognize specific 4-8 bp sequences
EcoRI 5GAATTC3
3CTTAAG5
PvuII 5CAGCTG3
3GTCGAC5
Restriction Enzymes for Transformation
Human DNA cleaved with EcoRI Corn DNA cleaved with EcoRI
5-C-G-G-T-A-C-T-A-G-OH PO4-A-A-T-T-C-A-G-C-T-A-C-G-3
3-G-C-C-A-T-G-A-T-C-T-T-A-A-PO4 + HO-G-T-C-G-A-T-G-C-5
5-A-C-G-G-T-A-C-T-A-G A-A-T-T-C-A-G-C-T-A-C-G-3
3-T-G-C-C-A-T-G-A-T-C-T-T-A-A G-T-C-G-A-T-G-C-5
5-A-C-G-G-T-A-C-T-A-G-A-A-T-T-C-A-G-C-T-A-C-G-3
3-T-G-C-C-A-T-G-A-T-C-T-T-A-A-G-T-C-G-A-T-G-C-5