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18/04/2016

Filamentous Bacterial Disease


fine, colorless, threadlike growth on body surface and gills of affected
Bacterial Diseases of species
Crustaceans Leucothrix sp.,Thiothrix sp., Flexibacter sp., Cytophaga sp.
Flavobacterium sp.
P. monodon, P. merguiensis, P. indicus, p. vannamei, Scylla serrata

Filamentous Bacterial Disease Filamentous Bacterial Disease


thick mat of filaments on egg surface microscopic examination of wet mounts
respiration, feeding, locomotion, molting may be impaired (larvae and maintain good water quality with optimum DO
PL)larval stages not as severely affected due to frequent molting low organic matter
respiratory distress leading to mortality due to hypoxia 0.15 ppm copper, 24h flowthrough
high organic load, low DO, stress from molting 0.5 ppm copper, 4-6h (PL2 and older shrimps)
80% mortality or more in a few days or week

Shell Disease, Brown/Black Spot, Black Rot/Erosion,


Blisters, Necrosis of Appendages
brownish to black, single or multiple, eroded areas on carapace,
abdominal segments, rostrum, gills and appendages
in larval and PL stages, the affected appendage shows a cigarette
butt-like appearance
blisters containing gelatinous fluid may develop on the carapace and
abdominal segments

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18/04/2016

Luminous Vibriosis Luminous Vibriosis


Vibrio harveyi, V. splendidus affected shrimp larvae becomes weak
opaque white juveniles lose their appetite
gram-negative, comma-shaped, luminescent colonies
swim to the pond surface and edges
all stages of shrimp heavily infected shrimp show continuous greenish glow when observed in
total darkness
opaque abdominal segments
hepatopancreas- target organ
inflammatory response in and around hepatopancreas
necrosis and dysfunction of hepatopancreas
up to 100% mortality
cause of Philippine shrimp production (mid 1990s)

Luminous Vibriosis Luminous Vibriosis


horizontal transmission bacterial isolation (NA, TCBS)
30 DOC- 10^4 CFU/hepatopancreas (threshold level); 10^2 infected tissues streaked on agar show lumiscent colonies
CFU/hepatopancrease (safe level) FAT
horizontal transmission ELISA
30 DOC- 10^4 CFU/hepatopancreas PCR-based detection method
Multiplex PCR

Luminous Vibriosis EMS/AHPND


in hatchery phase- regular monitoring
water and larvae
siphoning of sediments
low salinity culture system
Probiotics
green water culture system
crop rotation

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