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10 MRVP Dextrose For doing methyl red test and VP test as E. coli is MR +ve, acidic
medium end product pH <4.4, acetic, lactic and formic acids & Klebsiella is
VP +ve neutral end product pH> 6.0, acetyl methyl carbinol, most
enterobacteria gives either MR or VP test positive, but some as
Hafnia alvei and Proteus mirabilis can give both tests +ve
11 Nitrate Pot. Nitrate Enterobacteriaceae, vibrionaceae- positive, cherry red
broth Acinetobacter- negative
12 OF Glucose Bacteria Open Closed
medium Oxidation Enterobacteriaceae Acid gas or Acid Acid gas or Acid
fermentation Pseudomonadaceae Acid Alkaline
Acinetobacter Acid Alkaline
Vibrionaceae Acid gas or Acid Acid gas or Acid
13 Peptone Ferric citrate, H2S producer- Black coloration of medium in tube
iron agar Thiosulphate Non H2S producer- medium remains yellow
Differentiation of Vibrios
Character Yersinia Es
Pseudotu- Entero- Fredrikseni Intermedia Kristenseni Mollaretti Bercovieri
berculosis colitica
Colony size <1 mm <1 mm <1 mm <1 mm <1 mm <1 mm <1 mm >1
Lysine - - - - - - - V
decraboxylase
Ornithine - - + + + - - V
decarboxylase
Simmons citrate - except - V + - - - -
For Yersinia at serogroup
25oC IV
Urease + + + + + + + -
Indole - V + + V - - +
Phenyalanine - - - - - - - -
Gas from - V V V V V V +
glucose
Motility at 25oC + + + + + + + +
o
At 37 C - - - - - - - +
O
VP test at 25 C - + + + - - - -
Acid from - + + + + + +
cellobiose
Methyl-d- - - - + - - -
glucoside
Mucate - - V V - + +
Raffinose V - - + - - -
Rhamnose + - + + - - -
Sorbitol - + + + + + +
Sorbose - + + + + + -
Sucrose - + + + - + +
Pyrazinamidase* - V + + + + +
Pyrazinamidase test: A slant is made with TSA containing pyrezinecarboxamide and inoculated tubes
are incubated at 28oC for 48hr and then flooded with ferrous ammonium sulphate, which reacts with
pyrazinoic acid to give pink colour.
Wauters serotyping scheme is for Y. enterocolitica-18 serovars with 20 O antigens.
Biovars of Y. enterocolitica
Test 1e (environmental) 1a (american) 2 3
Hydrolysis of tween + + - -
Aesculin V + - -
Indole productiuon + + + -
Acid from xylose + + + +
Ornthine decarboxylase, nitrate + + + +
reduction and acid from trehalose
Pyrazinmidase + - - -
DNAse - - - -
Reservoir/ animal host Human and animals Humans, rats Human/pig/cat Chin
& fleas tle and sheep
Pathogenicity None (environmental) Human Human Chin
O Serovars associated with disease 4; 8; 13a; 13b; 9; 5,27 1,2,3
18; 20;21