ENDOLIMAX NANA

6 6-
CYST TROPHOZOITE

6- 10um 6-15um (ave. 7um) Tiny irregular shape

Mononucleated w/ eccentrically located karyosome

 Spherical to ellipsoidal (large)

Quadrinucleated w/ eccentrically located karyosome No peripheral chromatin Cytoplasm granular

(large)
Pseudopodia are blunt and hyaline
Cross eyed cyst
Movement is sluggish and random
Blotlike karyosome No peripheral chromatin

Lacks chromatoidal bodies

6-15um (ave. 7um) Tiny irregular shape

Mononucleated w/ eccentrically located karyosome

(large)

No peripheral chromatin Cytoplasm granular

Pseudopodia are blunt and hyaline

Movement is sluggish and random

Iodamoeba butschlii

CYST TROPHOZOITE

6-16um (ave. 9-10 um) Spherical to ellipsoidal 4-20 um (9-14um)

Mononucleated (large) w/ eccentrically located Irregular shaped

karyosome
Mononucleated w/ centrally located karyosome
Achromatic granules may or may not be present (large) surrounded by a cluster of achromatic granules

Most prominent feature: large compact mass of Motility: same w/ E. coli

glycogen (large iodine staining glycogen vacuole)
Granular , vacuolated cytoplasm w/c may contain
bacteria and
Entamoeba histolytica
CYST TROPHO
5-20um 10-60um
Rounded & elongated Irregular vary in size & form
Nuclei range from 1-4 w/ a minute centrally located Pseudopod: Dendritic and finger-like (release one at a
karyosome time or explosive)
Quadrinucleated Motility: Directional/ Unidirectinal & Progressive
Mature Endoplasm (granular) contains RBC (bluish black)
Chromatoidal bars w/ rounded ends but no bacteria
CB: Rod/cigar/ sausage/coffin shaped Single spherical nucleus consists of thin nuclear
membrane w/ fine, uniform chromatin granules and a
small centrally located karyosome
Entamoeba coli
CYST TROPHO
10-33um 15-50um (20-30um)
Usually spherical but may be elonggated Irregular vary in size & form
Nuclei range from 1-8 w/ an eccentric karyosome Pseudopod: Blunt and rounded
Peripheral chromatin: coarse and granular, and irregularly arranged along release several at a time
the nuclear membrane Motility: Non- directional &
In younger cyst chromatoidal bars (splinter like w/ pointed ends) and Slugish/ Non-progressive
glycogen mass may be present Endoplasm (granular) contains
CB: Spinter needles/ witch/whisked broomstick appearance Bacteria, yeast & cell dendritus
Single spherical nucleus consists
thicker nuclear membrane w/
variously sized, irregular
chromatin
granules and a large eccentrally
located karyosome

Acanthamoeba
C T
15-20 um 20-45 um
Spherical, and doubled walled, with the walls having Single nucleus w/ central prominent endosome
a Hyalin & blunt pseudopods w/
Wrinkled appearance Spine-like projections
Acanthamoeba castellanii of the cytoplasm (acanthopodia)

Giardia lamblia
C T
8-12um x 7-10um 9.5-12um x 5-15um
Ovoidal, foolball shaped Pear/teardrop shaped/ pyriform & w/a pair of
Cystic wall has 2 layers (double wall) ovoidal nuclei
Nuclei: 2 (young) and 4 (mature)
 Crossed fibrils arranged in parallel pairs Rounded anteriorly and pointed posteriorly
(bilaterally symmetrical)
Sucking disc occupies the ventral portion w/c
is pale stained shallow, slightly notched concavity
Prominent axostyle

Chilomastix mesnili
C T
7-10um x 4.5-6um 6-20um x 2-10um Pear-shaped Asymmetrical
Pear shaped or Lemon shaped Large spherical nucleus
Conical anterior with knob-like or nipple- shaped Longitudinal spiral groove in the surface in the middle
protuberance of the body
1 nucleus present Cytoplasm is grayish violet, sometimes with food
occasionally 2 vacoules
Cytoplasm is clearly separated from the cyst wall at Flagella are rarely seen Three anterior flagella
the anterior portion 1 Flagellum within the cytostome (cystostomal
fibril/sheperds crook)
Boring & spiral forward movement
Cork-screw movement
Trichomonas hominis
C T
5-14um x 7-10um Pyriform
A single nucleus at the anteror end w/a central karyosome
5 anterior flagella w/ one flagella on the margin on the undulating
membrane (full body length)
Undulating membrane as long as the costa
The posteror end of the axostyle protrudes through the posterior portion
of the parasite
Cytoplasm is grayish violet, finely granular
Trichomonas vaginalis
C T
7-32um x 5-12um (ave. 5-15um)
Pear shaped/Pyriform Undulating membrane
shorter
4 anterior flagella and the 5th along the margin of the undulating
membrane
Undulating membrane is 1/2 of the costa
Prominent axostyle
More granules along its axostyle and costa
Rapid jerky tumbling motility
Trichomonas tenax
C T
5-16um x 2-15 um
Small, pyriform flagellate w/c resembles T. vaginalis
Four free equal flagella & fifth on the margin of UM
Undulating membrane is 2/3 of the costa
Does not form cyst
MOT: Direct or indirect contact
through kissing, utensils and other
Dientamoeba fragilis
C T
3-22um (ave. 7-12um) Irregular
2 Nuclei (rosette shaped nuclei) w/ karyosome consisting of 4-6
discrete granules
Pseudopodia are broad, hyaline, and leaflike in appearance
Cytoplasm is granular w/ granules containing bacteria
Balantidium coli
C T
45-65um 50-200um x 40-70um
Spherical or ellipsoidal Pointed anteriorly and broadly rounded posteriorly
Thick, refractile cyst wall Macro (large & bean shaped) and micronucleus are
Macro and micronucleus are present present
Cytoplasm is granular Cilia may be present Cytostome located at the anterior end
Cytoplasm w/ vacoules
The entire body is covered w/ cilia
PLASMODIUM
Species Types of Malaria Interval Victims

Falciparum Malignant tertian 36-48 Hours All

Vivax Benign tertian 48 Hours Young

Malariae Quartan 72 Hours Adult

Ovale Benign tertian 48 Hours Young