Components Positive Result
Triple Sugar Iron Protein sources (Beef & Yeast
Agar (TSI) Extract, peptone)
Lactose, sucrose,
Glucose(1/10)
Phenol Red- indicator
Ferrous sulfate- indicator of
H2S
Indole Production Tryptophane broth
w/ p-
methylaminobenzaldehyde
Kovacs Reagent
Ehrlichs Reagent(Sensitive)
Methyl Red and MRVP Broth (Dextrose Broth
Voges-Proskauer butffered with peptone)
Tests (MR-VP)
Citrate Utilization Simmons Citrate Agar (SCA)
-Sodium citrate
Motility Test Semi-solid Agar
Sulfide Indole Motility
Medium(SIM)
Motility Indole Ornithine
Medium(MIO)
1% Triphenyltetrazolium
chloride (For visualization)
Negative Result Principle
Phenol Red-> Phenol Red-> Initial step in identification of
Yellow(Acid) Red(Alkaline) enteric bacilli.
Ferrous sulfate-> Aerobic slant position becomes
Black precipitate alkaline due to oxidative
decarboxylation.
Red No change Determine the ability of an
(Pink to wine organism to split tryptophan to
colored ring form indole.
(Book)) Used in ID of lactose-fermenting
enterics.
Red (Both MR & Yellow (MR & Determine the ability of an
VP) VP) organism to produce and
maintain stable acid end
products and produce neutral end
products from glucose
fermentation and to overcome
buffering capacity.
Bromothymol Determine if an enterobacter can
Blue-> Green to utilize citrate as main source of
Blue carbon and inorganic ammonium
salts as its only nitrogen source.
Spread growth in Growth at the Use to determine if an organism
the medium site of is motile and possess flagella.
inoculation
Urease Reaction Urea Agar Slant Pink- Red No color Change Determine the capability of an
(Light orange to organism to produce urease
Magenta(Book)) which hydrolyzes urea.

O-Nitrophenyl-Beta- ONPG disk Yellow colorless Detects the ability of organism

D-Galactopyranoside (Orthonitrophenol) to produce B-Galactosidase.
Reaction (ONPG) Determines presence of late or
slow lactose fermenting strains.

Decarboxylase Moeller Decarboxylase Purple-blue Yellow(Acid) Determines production of

Reaction Medium (1% Conc) (Alkaline) decarboxylases by bacteria.
Bromcresol purple indicator

Deaminase Reaction Lysine-Iron Agar (LIA) Colored compound Detects deaminase activity.
Phenylalanine or Tryptophan

Lysine Iron Agar Protein, glucose, lysine, sulfur Purple butt Yellow butt Determine the ability to
H2S Indicator Red slant deaminate lysine, decarboxylate
Bromcresol purple Black ppt. (H2S) lysine and produce hydrogen
sulfide gas.

Fermentation Media Peptone Medium with Pink Growth but no Determine the ability of an
Andrades Indicator color change organism to ferment a specific
carbohydrate.
Heart Infusion Broth with Yellow Growth but
Bromcresol purple indicator remains purple

Nitrate Reduction Nitrate broth Red w/ gas Detects the ability of an

Sulfanilic acid organism to reduce Nitrate.
Alpha-Naphthylamine