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FERR2
Ferritin Gen.2 - Application for C.f.a.s. Proteins Specific proteins
Order information
Analyzer(s) on which cobasc pack(s) can be used
COBASINTEGRA 400 plus
03528995 190 Ferritin Gen.2 (200 tests) System-ID 0768081
COBAS INTEGRA 800
11355279 216 Calibrator f.a.s. Proteins (5 1 mL) System-ID 0765570
10557897 122 Precinorm Protein (3 1 mL) System-ID 0791059
11333127 122 Precipath Protein (3 1 mL) System-ID 0791067
05117003 190 PreciControl ClinChem Multi 1 (20 5 mL) System-ID 0774693
05947626 190 PreciControl ClinChem Multi 1 (4 5 mL) System-ID 0774693
05117216 190 PreciControl ClinChem Multi 2 (20 5 mL) System-ID 0774707
05947774 190 PreciControl ClinChem Multi 2 (4 5 mL) System-ID 0774707
20756350 322 NaCl Diluent 9 % (6 22 mL) System-ID 0756350
English Mix all brand new (nonpunctured) cobasc packs for 1minute on a
cassette mixer before loading on the analyzer.
System information
Test FER2P, test ID 0278 COBAS INTEGRA 800 analyzers
After cobasc packs puncture, the analyzer automatically mixes the reagent
Intended use for 1minute.
In vitro test for the quantitative immunological determination of human
ferritin in serum and plasma on COBASINTEGRA systems. Storage and stability
Summary1,2,3,4,5,6,7,8,9,10 Shelf life at 28C See expiration date on
Ferritin is the iron storage protein. It has a molecular weight of cobasc pack label
440000daltons, depending upon the iron content, and consists of a
protein shell (apoferritin) of 24subunits and an iron core containing an COBASINTEGRA400 plus system
average of approximately 2500Fe3+ions (in the basic isoforms). Common On-board in use at 1015C 12weeks
to all isoforms is their construction from two separate subunits, the acid
H(heavy)type subunit and the weakly basic L(light)type subunit. The COBASINTEGRA800 system
basic isoferritins are responsible for the longterm iron storage function and On-board in use at 8C 12weeks
are mainly detectable in the liver, spleen and bone marrow. Acid isoferritins
are found mainly in the myocardium, placenta, tumor tissue and -to a Specimen collection and preparation
lesser content- in the depot organs. For specimen collection and preparation only use suitable tubes or
The determination of ferritin is necessary above all in iron metabolism collection containers.
diagnosis, monitoring iron therapy, ascertaining the iron reserves in groups The use of certain blood collection tubes containing separation gels and/or
at risk and in the differential diagnosis of anemias. It encompasses clotting accelerators may interfere with this test.
prelatent and latent iron deficiency as well as iron overloading. It is also
used to distinguish between hypoferric anemia and hypochromic anemia Only the specimens listed below were tested and found acceptable.
(chronic infection and tumor anemias, sideroblastic anemia or thalassemia). Serum
Plasma: Liheparin plasma, K2 or K3EDTA plasma
Ferritin determinations are particularly suitable for monitoring renal anemia
when iron utilization and distribution disorders are present during therapy The sample types listed were tested with a selection of sample collection
with erythropoietin. The ferritin detectable in blood is in equilibrium with the tubes that were commercially available at the time of testing, i.e. not all
bodys depot iron and hence acts as an indicator for the level of iron stores. available tubes of all manufacturers were tested. Sample collection systems
from various manufacturers may contain differing materials which could
A variety of methods are available for determining ferritin, e.g. affect the test results in some cases. When processing samples in primary
radioimmunoassay (RIA), enzymelinked immunosorbent assay (ELISA), tubes (sample collection systems), follow the instructions of the tube
fluorescence immunoassay (FIA), luminescence immunoassay (LIA) and manufacturer.
nephelometric immunoassay.
When using K3EDTA tubes pay particular attention that the tubes are
The automated Roche ferritin assay is based on the immunological adequately filled.
agglutination principle with enhancement of the reaction by latex.
Blood collected in capillary blood collection tubes is unsuitable. Do not thaw
Test principle frozen specimens in a 37C bath. Violent mixing may denature ferritin.11
Particle enhanced immunoturbidimetric assay. Do not use turbid samples.
Human ferritin agglutinates with latex particles coated with anti-ferritin Centrifuge samples containing precipitates before performing the assay.
antibodies. The precipitate is determined turbidimetrically at 552nm.
Reagents - working solutions Stability:12 7days at 1525C
7days at 28C
R1 Glycine buffer: 0.17mol/L, pH8.3; rabbit globulin: 5mg/mL;
stabilizers and preservative. 1year at (-15)(-25)C
SR Latex particles coated with antihuman ferritin (rabbit): 0.17%; Materials provided
glycine buffer: 0.17mol/L, pH7.3; stabilizer and preservative. See Reagents working solutions section for reagents.
R1 is in position B and SR is in position C. Materials required (but not provided)
Precautions and warnings NaClDiluent9%, Cat.No.20756350322, systemID0756350 for
automatic sample dilution and standard serial dilutions. NaClDiluent9% is
Pay attention to all precautions and warnings listed in placed in its predefined rack position and is stable for 4weeks onboard
Section1/Introduction of this Method Manual. COBASINTEGRA400plus/800analyzers.
Reagent handling
Ready for use
COBAS INTEGRA 400plus analyzers
FERR2
Ferritin Gen.2 - Application for C.f.a.s. Proteins Specific proteins
FERR2
Ferritin Gen.2 - Application for C.f.a.s. Proteins Specific proteins
FERR2
Ferritin Gen.2 - Application for C.f.a.s. Proteins Specific proteins
Symbols
Roche Diagnostics uses the following symbols and signs in addition to
those listed in the ISO 152231 standard.
Contents of kit
Volume after reconstitution or mixing
GTIN Global Trade Item Number