Brix Refractometere51709840

RE20B BRIX Refractometer
Operating Instructions
Contents
Contents Page
1. Introduction ..................................................................................................... 5
2. Safety measures .............................................................................................. 6
3. BRIX, HFCS and Refractive Index .................................................................. 7

3.1 Definition of refractive index ............................................................................... 7
3.2 Definition of BRIX (%) ........................................................................................ 8
3.3 Definition of HFCS42 and HFCS55 (Inverted sugar) ......................................... 8
3.4 Measurement principle ....................................................................................... 9
4. The instrument .............................................................................................. 10

4.1 General overview ............................................................................................. 11
4.2 Keyboard .......................................................................................................... 12
4.3 Display ............................................................................................................. 14
4.4 Measures for correct measurements ............................................................... 15
5. Getting started ............................................................................................... 16

5.1 Setting up the instrument ................................................................................. 16
5.2 Performing the first adjustment (calibration) .................................................... 17
5.3 Perform a measurement (BRIX, HFCS42 and HFCS55) ................................. 19
6. Sample (Number and ID) .............................................................................. 20
7. Report ............................................................................................................. 20
8. Functions (password protected) ................................................................. 21

8.1 Data File ............................................ (Function 0) .......................................... 21
8.2 Set check .......................................... (Function 1) .......................................... 23
8.3 Periodic Check .................................. (Function 2) .......................................... 25
8.4 Periodic adjustment, resp. calibration (Function 3) .......................................... 26
8.5 Memory Clear .................................... (Function 4) .......................................... 27
8.6 Wait Time .......................................... (Function 5) .......................................... 27
8.7 Decimals ........................................... (Function 6) .......................................... 28
METTLER TOLEDO RE20B BRIX Refractometer 3

Contents
Page
9. Setup (password protected) ......................................................................... 29

9.1 Interface ............................................ (Setup 0) ............................................... 29
9.2 Date and Time ................................... (Setup 1) ............................................... 30
9.3 Name ................................................. (Setup 2) ............................................... 31
9.4 Serial/Version number ....................... (Setup 3) ............................................... 31
9.5 LCD Contrast .................................... (Setup 4) ............................................... 31
9.6 International ...................................... (Setup 5) ............................................... 32
9.7 Lock with password ........................... (Setup 6) ............................................... 32
9.8 Beep .................................................. (Setup 7) ............................................... 33
10. Error messages and malfunctions .............................................................. 34
11. Standard and optional equipment ............................................................... 35

11.1 Standard equipment ......................................................................................... 35
11.2 Optional equipment .......................................................................................... 35
MemoCard RE20B (Short Instructions) ........................................................................ 36
Annex 1: BRIX Table ...................................................................................................... 37
Annex 2: Temperature corrections for BRIX refractometer values ......................... 38
Specifications ................................................................................................................. 40
4 METTLER TOLEDO RE20B BRIX Refractometer

Introduction
1. Introduction
The RE20B BRIX is a refractometer specially designed for quick measurement of BRIX and
concentration of isomerized (invert) sugar solution (HFCS42, HFCS55) with a very high
accuracy. Its measurement range is from 0 to 100 %, with the BRIX values higher than 85 %
being extrapolated from the recognized ICUMSA table.
The BRIX measurement gives a result directly in percentage of sucrose in water. The
HFCS42 and HFCS55 give the result in percentage of High Fructose Corn Syrup, the
HFCS42 being used for solutions with 42% fructose, the HFCS55 for solutions with 55%
fructose.
The measurement is performed at the actual sample temperature and the result compensat-
ed to 20 C according to the ICUMSA table.
Features of RE20B:
The light source for measurement is an LED which is maintenance free (unlike conventional
light bulb that blows from time to time).
The measurement prism is made of sapphire, which is strongly resistant against corrosion,
very rigid and of high thermal conductivity.
The border temperature between sample and prism is measured so that highly reliable data
can be expected and obtained as measurement results.

Safety measures
2. Safety measures
The instrument has been tested for the experiments and intended purposes documented in
the Operating Instructions. However, this does not absolve you from the responsibility of
performing your own tests of the product supplied by us regarding its suitability for the
methods and purposes you intend to use it for. You should therefore observe the following
safety measures.
Measures for your protection

Ensure that you plug the power cable supplied into a socket which is
grounded! A technical defect could be lethal in the absence of grounding.
Switch the instrument off and disconnect the power cable if you change
blown fuses! An electric shock could be lethal.
Do not work in a hazardous area! The housing of the refractometer is not

gas tight (explosion hazard through spark formation, corrosion by gas
diffusion into the instrument).
Measures for operational safety

Do not add samples with hard objects made of glass, metal or ceramics!
They could damage the prism.
Clean the prism immediately after measuring strong acids and bases!
These could attack the prism.
Use only fuses of the specified type if you need to change them!
Exclude the following environmental influences:
powerful vibrations,
direct sunlight and sharp changes in temperature,
exposure to corrosive gases,
very dusty places,
high atmospheric humidity,
temperatures below 5 C and above 35 C,
powerful electric or magnetic fields which influence the power supply
through large load fluctuations!
If the instrument is not going to be used for a longer period of time, clean
the prism before stored. It is recommended to use the carton box in which
it was first delivered.
Avoid a place for storage under excessive ambient condition including too
high or too low temperature, high humidity or heavily dusty area.

Refractive index
3. BRIX, HFCS and Refractive Index
3.1 Definition of refractive index

The refractive index n of a substance is the ratio of the velocity of a ray of light in vacuum to
its velocity in the medium (dimension = 1).
If a ray of light at a particular angle passes

from, e.g. air to water (optically less dense
to optically more dense medium), it chang-
n1 e.g. es its direction except when the incident
of air
light is vertical. According to Snell's law of
refraction, the ratio of the refractive indices
of the two media is proportional to the ratio
of the angle of refraction and angle of inci-
n2 e.g.
of water dence of the ray of light:
n1 sin
=
n2 sin
Air If a ray of light passes into an optically less

dense medium from an optically more
dense medium, it also changes its direction.
If the angle of incidence is increased, it
reaches a critical value at which the ray of
Water light no longer passes into the optically less

dense medium, the angle of refraction
= 90 C. If this "critical angle" is exceeded,
total reflection occurs. The critical angle is
Critical angle Total reflexion used to calculate the refractive index:
= 90 sin = 1
n2
n1 =
sin
As the refraction depends on the wavelength of the incident light, the refractive index is
measured at the wavelength of the D line of sodium (589.3 nm) as standard and symbolized
by nD.
The refractive index depends not only on the wavelength used to measure it, but also on the
temperature of the solution being measured. The standard temperature is 20 C. If measure-
ments are performed at a different temperature, e.g. 25 C, this must be specified: n D25.

Refractive index
3.2 Definition of BRIX

The BRIX degree represents the concentration in weight percent of a mixture of pure
sucrose and water (g sucrose per 100 g solution). It is used as the weight % concentration of
sucrose in general. This concentration is calculated from the measured refractive index
using the stored concentration table (16th conference of the International Commission of
Uniform Methods for Sugar Analysis ICUMSA). The result is given at the standard reference
temperature of 20 C using the temperature dependence for these solutions from the same
source.
So the refractive index and the prism surface temperature are measured and the BRIX is
calculated from the tables.
Note: When substances other than sucrose are present, BRIX is calculated from the total
refractive index. In such a case the result in BRIX will not indicate the real sucrose
concentration.
3.3 Definition of HFCS42 and HFCS55 (Inverted sugar)

HFCS stands for High-Fructose Corn Syrup. This syrup is manufactured from natural corn
syrup and consists of inverted (or isomerized) sugar: a mixture of dextrose, fructose, maltose
and sucrose. They are classified according to their fructose content. Mainly three different
HFCS are in use: a mixture containing 42% fructose (HFCS42), one containing 55% fructose
(HFCS55) and a third one containing 90% fructose (HFCS90).
Inverted sugar concentration denominates the concentration in weight % of an isomerized

sugar solution. Inverted sugar concentration can be converted from the refractive index of
20
the inverted sugar solution measured at 20 C (n D ). The RE20B stores conversion tables
(Physical Properties Table, Corn Products, 1991) for two of the mixtures: HFCS42 and
HFCS55. Since a temperature compensation table is also stored, temperature compensa-
tion is practicable, as long as the sample temperature is within the range of 20 ~ 60.
The relation between inverted sugar concentration and refractive index varies with the
composition of the inverted sugar. Compositions of the inverted sugars HFCS42 and
HFCS55 stored in the RE20B are shown below.
HFCS42 HFCS55
Fructose 42.50% 55.40%
Dextrose 52.50% 40.30%
Maltose 3.00% 3.00%
Sucrose 0.00% 0.00%
Maltotriose DP3 0.70% 0.40%
Oligosaccharide DP4 1.30% 0.90%
Sulfated Ash 0.03% 0.05%

Refractive index
3.4 Measurement principle
The light emitted by the RE20B pass-

es through the prism and encounters
the solution. It is partially refracted
(angle of incidence < critical angle)
and partially reflected (angle of inci-
dence > critical angle).
The reflected light is recorded using
an optical sensor (CCD). The bounda-
ry between the dark and light areas
represents the critical angle needed to
calculate the refractive index.
Setup of the measurement system

The light source is a light emitting di-
ode (LED) whose beam passes
through a polarizing filter, an interfer-
ence filter (589.3 nm) and various
lenses before it passes through the
sapphire prism and encounters the
sample. The reflected light (angle >
critical angle) is led via a lens to the
optical sensor, which records the criti-
cal angle.
The temperature at the boundary re-
gion prism-sample is measured by the
embedded sensor.

The instrument
4. The instrument
Front view
3. Cover lid
4. Measuring cell
5. Prism
1. LCD display
2. Keypad
Rear view
6. Printer connector
7. RS-232 connector
8. Power switch
9. AC power connector

The instrument
4.1 General overview

1. LC Display
The RE20B has a two-line liquid crystal display that shows measurement results, condi-
tions or parameters.
2. Keypad
Start measurement, input of measurement conditions or calculation parameters are
operated on these keys.
3. Cover lid
It covers the sample stage for protection and prevents incoming dispersed light. When
opened it will prevent sample liquid from splashing to the rear of the instrument.
4. Measuring cell
This is the measurement cell. Sample solution is dropped to the centre of the cone. The
sample drops must fill the indicated line which shows approximately 0.4 mL.
5. Prism
The prism is made of sapphire, which is resistant to corrosion and physical damage.
6. Printer connector
This is for connection of RE20B to an external printer.
7. RS-232C connector
This is for connection of RE20B to a personal computer.
8. Power switch
The power of RE20B is turned on or off by this switch.
9. AC power connector (IN)

Power is supplied by connecting the supplied power cord.
Connect the power cable supplied to the power supply. The instrument will automatically
adjust its power supply unit to the specific line voltage of your country as long as this is in the
range between 100 and 240 V AC.

The instrument
4.2 Keyboard
Display Sample
Print Report
Func.
Setup
7 8 9 Check Calib. BS Method

ABC DEF GHI
4 5 6
JKL MNO PQR
1 2 3 Enter
STU VWX YZ
. 0 - Clear Esc. Reset Measure

BLK
Description of each key and its function:
Sample
Sample Key to set up sample parameters, like sample number and ID/Lot.
Report Report Key to change print format.

There are three (3) kinds of format for printing.
(1) Short: Short form for printing minimum necessity of resulting data.
(2) GLP: GLP form, which complies with GLP requirements.
(3) Variable: User defined printing format.
Method Method Key to change Method.

(1) Method 0: BRIX concentration
(2) Method 1: Concentration of isomerized sugar HFCS42
(3) Method 2: Concentration of isomerized sugar HFCS55
Display Display Key to change display or selectet numeric, capital or small letters
Print Print Key to print the displayed message or parameters

The instrument
Function Function Key to selectet following functions:

(0) Data File: Recalculation, auto statistics, deletion of measure-
ment results
(1) Set Check: Define the names, values and allowed tolerances
for the standards to be used for daily and precision
check of the instrument
(2) Periodic Check: Define the interval for the precision check
(3) Periodic Calib: Define the interval for calibration
(4) Memory Clear: Initialize stored data
(5) Wait Time: Select time to wait for measurement after [Meas-
ure] key is pressed
(6) Decimals: Select a number of digits after decimal
Setup Setup Key to select the setup parameters as follows:

(0) Interface: Set up the RS-232C and the printer
(1) Date & Time: Set up date and time
(2) Name: Register name (lab or operator)
(3) Serial/Ver. No.: Display serial and version No. of the unit
(4) LCD Contrast: Adjust brightness of LC display
(5) International: Select format for date and select unit (C/F) for
temperature
(6) Lock: Set up a password for protection
(7) Beep: Select beep at the end of measurement or cancel it
Check Check Key to start the instrument check, daily (always) or precision (only
when due)
Calib.
Calibration Key to start a calibration
BS
Backspace Key to erase one preceding letter or numeral
Clear
Clear Key to erase the whole entry (2x to clear names)
Esc.
Escape Key to return to the main display
Reset
Reset Key to abort a measurement or a calibration and to return to main
display
Measure Key to start a measurement

The instrument
7
ABC 0 9 key Key to enter alphanumeric characters (the selection between
capital/small letters or numbers is done with the <DISPLAY>
key). To select B, press twice, for C press three times.
On the key with the number 0 there BLK (BLK = blank).
. Dot and hyphen key

Enter Key to confirm a entry. This key is also called <Enter> key in
Enter
this manual.
Up

Left, Right
Keys to move the cursor.
Down

4.3 Display
The RE20B BRIX meter has a two line LCD display with 15 characters per line. When the
power is turned on (power switch on the rear of the instrument), the instrument will diyplay
the following message for a few seconds:
RE20B Vx.xx The software version appears for a short time. This soft-
Brix Meter ware version can also be displayed under Setup 3 "Seri-
al/Version".
0 Ready After a few seconds the main display will appear:

27.92 C Brix Then it will switch to the ready mode.
The number in the left top corner shows the selected
method, i.e. method "0".

The instrument
4.4 Measures for correct measurements

Check the instrument from time to time measuring reference standards, e.g. from R.P.
Cargille Laboratories, Inc. USA.
Use soft paper towels or washed gauze to remove the sample or dry the prism.
Use deionized water to clean the cell after aqueous samples. For organic samples use a
suitable organic solvent like toluene, ethanol or acetone. If the solvent used doesn't
evaporate readily enough, use a second solvent like acetone to wipe the first one off and get
a dry cell.
Never use cotton wool as its fibres could adhere to the prism and influence the measure-
ment!
Fill the sample until the ring mark is covered (approximately 0.4 mL)
Always stir the sample on the prism carefully to allow air bubbles to escape!

Getting started
5. Getting started
5.1 Setting up the instrument

5.1.1 Printer (Setup 0)
If you work without printer you can skip this section and go directly to section 5.1.2. Perform
the following steps if you have a GA42 printer to connect:
- Make sure that the DIP switch 2 is ON on the GA42 printer.
Press the <SETUP> key
Interface? Select "0.Interface". Confirm with <ENTER>.

Printer Select "Printer" with the key. Confirm with <ENTER>.
Printer? Select "GA" with the key. Confirm with <ENTER>.

GA
To choose the format of the printed results press the <REPORT> key and choose between
"None", "Short", "GLP" and "Variable" (see section 7 "Report").
Then press the <RESET> key.
5.1.2 Date and time (Setup 1)
Press the <SETUP> key
Setup(0-7)? 1 Select "1.Date&Time". Confirm with <ENTER>.

Date&Time
Date & Time: Actual date and time are displayed. Press <ENTER>
key.
15/04/199811:27
Enter date (the date format is selected in the menu Setup

Date DD/MM/YYYY? "5.International") and confirm with <ENTER>
20/06/1998
Time? Enter the correct time and press <ENTER>.
11:27

Getting started
5.1.3 Enter the lab name (Setup 2)

Press the <SETUP> key.
Setup(0-7)? 2 Select "2.Name". Confirm with <ENTER>.
Name
The capital A means that the alphanumerical keyboard
Name? A is in entry mode for capital letters. Press the <DISPLAY>
key to change the entry mode:
A: capital letters (A, B, C, D, ..)
a: small letters (a, b, c, d, ...)
Name? 1 1: numbers (0, 1, 2, 3, 4, ...)
Quality Lab 1
Enter the lab name or the instrument name using the alphanumeric keys, e.g. Quality Lab
1, and confirm with <ENTER>.
The instrument is now ready for calibration and the first measurements.
5.2 Performing the first adjustment (calibration)

After turning on the instrument allow at least 15 minutes for the instrument to warm up before
performing the first adjustment.
Note: According to the International Vocabulary of Basic and General Terms in Metrol-
ogy from DIN the operation of bringing a measuring instrument into a state of
performance suitable for its use is called adjustment.
The earlier used term calibration should not be used anymore for this purpose as
soon as instrument settings are changed or adapted in order to ensure correct
measurement values.
Clean Prism Press the <CALIB.> key and follow the directions on the
display.
Press Enter Key
Open the cover lid and clean the measuring cell (the
prism) thoroughly with water and ethanol. Then wipe it
dry with a soft tissue, like soft paper towels or washed
gauze. Then close the lid and press <ENTER>. While
adjusting to the air value the display shows shortly:
Calibrating before switching to

25.67C

Getting started
Set Water Add water to the measuring cell until the ring mark is
covered (approximately 0.4 mL). After pressing <EN-
Press Enter Key
TER>, the following message will be flashing in the
display during adjustment to the water value.
Calibrating
25.72C
When the adjustment is done the display shows this
Calibration message.
27.61C OK
[Calibration] The results of the adjustment are printed on the attached

Date : 29/06/1998 11:15 printer.
Result : OK
Temperature : 27.61 C The "OLD" value is the measured value of the actual
water with the old adjustment settings.
OLD > NEW
Water The "NEW" value is the tabulated value of water as-
0.00 0.00 signed to the actual reading.
Name : Quality Lab 1 These values of the last 10 adjustments) are stored and
can be viewed and printed in the list of adjustments (see
---------------- chapter 8.4).
Calibration When the instrument fails to do the adjustment it will

27.61C Failed show an error message.
In such a case clean the prism well, wipe it dry, and repeat the adjustment. If it fails again,
then exchange the pure water (make sure it really is of the appropriate quality, i.e. bi-distilled
or correctly deionized).
If the adjustment still does not succeed, contact your METTLER TOLEDO service for
technical assistance.
5.3 Perform a measurement (BRIX, HFCS42 and HFCS55)

Press <METHOD>.
Method No.? 0 Choose the correct method by using the up and down
Brix arrows from the list:
- Method 0: BRIX,
- Method 1: Solid (HFCS42),
- Method 2: Solid (HFCS55).
Confirm with <ENTER>.

Getting started
Now perform for example three measurements with your standard sample.
Press <SAMPLE> to enter the sample data: sample number and sample ID.
Sample No.? Enter sample no. 02-01: press 2 <ENTER> 1 <ENTER>.

[02]- [01]
Sample ID? A Then you can enter your sample ID using the alphanu-
meric keys (max. 10 letters) and confirm with <ENTER>.
Orange J._
Add your sample to the measuring cell until the ring mark is covered (approximately 0.4 mL)
and stir carefully to remove any air bubbles from the surface of the prism.
Close the cover lid and press <MEASURE> (the green key).
Measuring The display is flashing during measurement.

29.85C 11.23%
0 Brix When the measurement is finished, the result is dis-

29.81C 11.21% played and printed.
Example of a GLP print of such a BRIX measurement.

*** R e s u l t ***
Sample No. 02-06
Date : 29/06/1998 12:07

Meas.Temp. : 29.81 C
Brix(20.00 C) :
11.21%
Name : Quality Lab 1
----------------

Sample and Report
6. Sample (Number and ID)
Here you can define your sample identifications like sample number and sample ID.
The sample number is composed of two parts: for example [03][01].

The first two-digit number ranging from 00 to 98 represents the series number of your
measurement series. In the instrument it is called "High Sample No.". If you group your
measurements of different sample types by this series number, you can conveniently
process your data statistically later on.
The second two-digit number ranging from 00 to 99 is automatically increased by one per
measurement.
Additionally you can define a sample ID, for example a sample name or the Lot number.
Press the <SAMPLE> key.
Sample No.? Enter the sample ID using the alphanumeric keys (max.
[03][01] 10 characters including "space", "period" and "hyphen".
Sample ID? A
Lot 36-05a
7. Report
In the <REPORT> menu you can choose the format of your printout according to the
following table.
Short GLP Variable
Name X X X
Model/Serial X choice
Sample No. X X choice
Date & Time X X choice
Sample ID X choice
Method No. & Name X choice
Meas.Temp. X choice
Result X X choice
Meas. Time X choice

Functions
8. Functions (password protected)
8.1 Data File (Function 0)

This function allows to view and process the measured data. You can either do a recalcula-
tion of measured results, statistics on the results or delete single data i.e. mark them so that
they are not considered in the statistics calculations.
Note: To view stored measured data, use the "Data deletion" function and skip the delete
step.
Function(0-6)?0 Select <FUNCTION> 0 and confirm with <ENTER>.

Data File
Now select with the and keys between <Statistics>

Data File?
or <Data Deletion> and confirm with <ENTER>.
Statistics
8.1.1 Statistics
The RE20B BRIX meter allows to do a statistical evaluation of your data. You can calculate
the mean value (Mean), the standard deviation (SD) and the relative standard deviation
(RSD). The relative standard deviation is the same as the coefficient of variation (CV).
The measured data can be filtered so that useful statistical results can be obtained after a
days work. On the first level the method (0 for BRIX, 1 for HFCS42, or 2 for HFCS55) will give
access to the respective data only. On the second level the series number (first two-digit
number of the sample number) can be chosen for which the statistics is to be done.
Method No.? 0 After selecting <Statistics> and confirmation with <EN-

TER>, the display asks for the method number (0 for
Brix
BRIX, 1 for HFCS42, or 2 for HFCS55) for which you
want to perform the statistics. Confirm with <ENTER>.
Next it asks for the High Sample No.: that is the series
High Sample No.?
number, the first two-digit number of the sample number.
01 Confirm with <ENTER>.

Functions
Data Print? Now you select whether the statistics results are to be
Off printed alone ("Off") or together with a list of all the
measured values ("On"). Confirm with <ENTER>.
Results: 4 The first part of the statistics is now shown on the display.
Mean : 11.21% With the down arrow you can switch to the second part
i.e. standard deviation and relative standard deviation.
SD : 0.06%
RSD : 0.54%
<Statistics>
To print the results of the statistics calculation press the
Date : 29/06/1998 17:29 <PRINT> key.
Sample No.(High) : 01
Method No. : 0(Brix)
Results: 4
Mean : 11.21 To get back to the beginning of the statistics selection
SD : 0.06 press the down arrow.
RSD : 0.54
Press <RESET> to exit the functions.

Name : Quality Lab 1
----------------
8.1.2 Data Deletion

This function allows to exclude single measured values from the statistics calculation by
marking them with an asterisk.
Data File? Select data deletion with the and keys and confirm
Data Deletion with <ENTER>.
Deletion No. Now enter one of the numbers of the given range (for
(00-06)?06 example 00) and press <ENTER>.
01-05 Orange J. You can flip through the list of the selected measurement
00 11.38% using the and keys.
01-05 Orange J. Pressing the <DISPLAY> key will turn on and off an
00* 11.38% asterisk on the selected measurement number.

Functions
Now you can resume the statistics calculation again and then print the results without the
marked measurements. If you selected the data print "On" the excluded measurements will
be printed with an asterisk before the sample number.
8.2 Set check (Function 1)

In this function you can set up the substances - name, rated value and allowed tolerances -
you want to use as standard check samples either for the daily or the periodic check.
Select function 1 - Set Check - and confirm with <ENTER>.
Set Check? With the and keys select either "Daily" or "Periodic"
Daily and confirm with <ENTER>.
8.2.1 Set Daily Check

This check is used mainly to control the working condition of the instrument. Is the instrument
really clean and dry? Did the previous operator really clean the cell after measuring his
sample?
Pressing the check key will start a periodic check only when such a test is due according to
the settings in section 8.3. When no periodic check is due it will start a daily check.
Normally deionized or bi-distilled water is used for this purpose. Of course any other solution
can be used that suites the purpose i.e. is readily and in abundance available as well as has
a stable and well known composition.
Enter the name of the check solution (max. 10 charac-

Standard? A ters) using the alphanumeric keys.
Water
Enter the rated value of the check solution and confirm
Ref.Value? with <ENTER>.
0.00%
Enter the allowed tolerances for this solution and confirm
Tolerance? with <ENTER>.
0.15%
The results of this daily check are not stored in the instrument. They are just meant to check
whether the instrument is really ready for the next measurement.

Functions
8.2.2 Set Periodic Check

This check is used mainly to verify whether a new adjustment of the instrument is necessary
or not. Every adjustment of the instrument means that the instrument settings are changed.
This can be dangerous if it is not done correctly because all the measurements afterwards
will be wrong.
Pressing the check key will always activate a daily check, unless a periodic check is due (see
also section 10.6). When no periodic check is due it will start a daily check.
So instead of actually performing frequent adjustments we suggest to perform frequent

checks with an exactly known standard solution. As long as this periodic check is done
successfully, there is no need to adjust the instrument. The results of period checks are
stored automatically in the instrument.
Should the periodic check fail, the operator should carefully clean and dry the measuring cell
and then repeat the periodic check.
If it still fails, then the instrument should be adjusted again: press the <Calib.> key and follow
the instructions of the instrument.
If the periodic check fails too often - like every second or third time - then you should contact
your METTLER TOLEDO office for servicing and closer support.
A standard solution can be a commercially available standard of well-defined accuracy (a

certified reference standard) or a standard that is prepared in-house strictly according to an
SOP.
For example a solution of 10.00% sucrose in doubly distilled water - i.e. weigh 10.00 g
sucrose into a volumetric flask and fill up with doubly distilled water to exactly 100.00 g -
could be used for this purpose.
Select "Periodic" under function 1 (with the <Down> key) and confirm with <ENTER>.
Standard? A Enter the name of the check solution (max. 10 charac-

10 Brix ters) using the alphanumeric keys.
Ref.Value? Enter the rated value of the check solution and confirm
10.00% with <ENTER>.
Tolerance? Enter the tolerances you want to allow for this solution
0.06% and confirm with <ENTER>.

Functions
8.3 Periodic Check (Function 2)

This function is used to set the intervals of the periodic check. This function is also used to
display and print the last check data stored in the instrument.
The display will show the due date for the next periodic
Next Check Date:
check. This due date is calculated automatically by the
06/07/1998 instrument according to following settings. Confirm with
<ENTER>.
Select the periodic check (Check Alarm to be "On" or

Check Alarm? "Off", and confirm with <ENTER>.
On If "On" is selected, the instrument will give one of the
following messages when it is turned on:
"X Days To Check" as a remainder, "Check Day" on the
due date, and "Check Day Over!" on the following days.
Each of these displays will return to the "Ready" display
after a while.
If "Off" is selected, the instrument will not remind you to
check.
Check Interval? Enter the check interval (in days) and confirm with <EN-
TER>.
_ 7Day
Note: Enter 0 Day to force a periodic check on today's
date. This will especially be required if the peri-
odic check has failed, in order to perform it again.
Select "Yes" to display the list of the last 10 checks

Show Check List?
performed. The whole list can be printed by pressing the
Yes <PRINT> key.
01; 10 Brix OK When "Yes" is selected and confirmed with <ENTER>,

the last check result is displayed. You can switch through
29/06 10.00%
the other check results using the and keys.
The whole list can be printed pressing the <PRINT> key.
Data not exist. If there are no such data (none performed yet or data
Press Any Key! cleared previously), this message starts flashing. Press-
ing any key will return the screen to "Function(0-6)?".
The results of such a periodic check are automatically stored in the instrument. You can view
the last 10 checks performed as described above.

Functions
Note: Once the periodic check has been performed the CHECK key will automatically
switch to the daily check. In order to repeat a periodic check, set the Check Interval
to 0 Day to force a periodic check on today's date. This will especially be required if
the periodic check has failed, in order to perform it again.
8.4 Periodic adjustment, resp. calibration (Function 3)

This function is used to set the intervals of the periodic adjustment (calibration). This function
is also used to display and print the last adjustment data stored in the instrument.
The instrument has to be adjusted from time to time. You can either choose the time
according to your special needs i.e. perform it when the periodic check fails for example two
times successively, or you can define a certain time period after which the instrument will
remind you to repeat the adjustment.
The results of these adjustments will automatically be stored by the instrument. You can view
at any time the last 10 adjustments performed and also print out the list for documentation
purposes as described below.
Select function 3 "Periodic Calib." and confirm with <ENTER>.
Next Calib.Date: The display will show the due date for the next periodic
adjustment. This due date is calculated automatically by
06/07/1998 the instrument according to following settings. Confirm
with <ENTER>.
Select the periodic adjustment to be "On" or "Off", and
Calib.Alarm?
confirm with <ENTER>. If "On" is selected, the instru-
On ment will give one of the following messages when it is
turned on:
"X Days To Calib" as a remainder, "Calib. Day" on the
due date, and "Calib. Day Over!" on the following days.
Each of these displays will return to the "Ready" display
after a while.
Calib.Interval? Enter the periodic adjustment interval (in days) and con-
_ 7Day firm with <ENTER>.
Note: Enter 0 Day to force a periodic adjustment on
today's date.
Select "Yes" or "No" with the key whether or not the list
Show Calib.List? of the last 10 adjustments performed is to be displayed.
Yes The whole list can be printed by pressing the <PRINT>
key.

Functions
01;29/06/1998 OK When "Yes" is selected and confirmed with <ENTER>,

0.00% 0.00% the last adjustment data is displayed. You can switch
through the other adjustment data using the and
keys.
Data not exist. If there are no such data (none performed yet or data
Press Any Key! cleared previously), this message starts flashing. Press-
ing any key will return the screen to "Function(0-6)?".
8.5 Memory Clear (Function 4)

This function is used to reset all parameters to default (factory setting), for instance to
initialize the memory after a serious malfunction of the instrument.
You can also selectively reset single records like the adjustment data (Calib. data), the check
records, the sample files or the method parameters.
Memory Clear? Press <Function> 4 and confirm with <ENTER>.

Measured Data
Select the desired option with the and keys:
"Measured Data", "Sample File", "Periodic Check", "Peri-
odic Calib.", "Method Para.", "All Parameter", and con-
Clear Execute? firm your choice with <ENTER>.
Yes
Select "Yes" to confirm your choice again and press
<ENTER>.
The chosen data or parameters are now deleted and the respective factory settings are
active again.
8.6 Wait Time (Function 5)

If the sample temperature is too far from the prism temperature, a measuring error is likely to
occur because of a sharp temperature change when the sample is applied to the prism
surface. To prevent this problem you can define a wait time in seconds. When you press
<Measure> the internal clock of the instrument first counts back this time before actually starting
the measurement. Thus the sample has time to adapt roughly to the prism temperature.
Function(0-6)?5 Press <FUNCTION> 5 <ENTER>.

Wait Time
Enter the wait time (in seconds) and confirm with
Wait Time?
_ 10s <ENTER>.

Functions
8.7 Decimals (Function 6)

Here you can select the number of decimals you want to show in the results of your
measurements.
Function(0-6)?6 Select <FUNCTION> 6 and confirm with <ENTER>.

Decimals
Enter the number of decimals for the result display:
Decimals choose between 0, 1 or 2. Confirm with <ENTER>.
(0-2)? 2
Note: Since the RE20B BRIX meter in fact has only one method that allows three different
result calculations i.e. BRIX, HFCS42 and HFCS55 the wait time as well as the
decimals in result display will be valid for all measurement that are performed after
defining it. When a different wait time is needed, you have to set it up anew.

Setup
9. Setup (password protected)
9.1 Interface (Setup 0)

This option is used to setup the RS-232C serial interface and the serial printer.
Setup(0-7)?0 Select "0.Interface". Confirm with <ENTER>.

Interface
Interface? Select "Printer" with the -> key in order to setup the
Printer printer or "RS-232C" to setup the serial interface
9.1.1 Printer setup

We recommend to select the GA42 printer from METTLER TOLEDO. Make sure that the DIP
switch 2 is ON on the GA42 printer (see operating instructions of the GA42).
Following printers are available:

Printer? GA- : for GA42 and SPRINTER printer from
METTLER TOLEDO.
GA
Other: for all serial printers. Selection of:
- Baud rate (300, 600, 1200, 2400,....)
- Parity (none, even, odd)
- Stop bits (1, 2)
- Data bits (7, 8)
These parameters have to be set according to
the specifications of your printer.
The bold settings are active for the GA42 printer.
IDP- : not used
DP- : not used

Setup
9.1.2 RS-232C Interface setup

This option is used to set the RS-232C interface for connecting a computer. Make sure that
the computer is set up with the same values.
Interface? Select "RS-232C" with the key. Confirm with

RS-232C <ENTER>.
Baud Rate? Select the following values according to your computer

1200 setup.
Parity?
None
9.2 Date and Time (Setup 1)

This option is used to enter the correct date and time.
Setup(0-7)? 1 Select "1.Date&Time". Confirm with <ENTER>.

Date&Time
Date&Time? Actual date and time are displayed. Press <ENTER>

1998/06/30 14:56 key.
Date DD/MM/YYYY? Enter date (the date format is selected in the menu Setup
20/06/1998 5.International) and confirm with <ENTER>.
Time? Enter the correct time and press <ENTER>.

11:27

Setup
9.3 Name (Setup 2)

This option is used to enter the laboratory (or company or operator) name.

Select "2.Name". Confirm with <ENTER>.
Setup(0-7)? 2
Name
The capital A means that the alphanumerical keyboard
Name? A
is in entry mode for capital letters. Press the <DISPLAY>
key to change the entry mode:
A: capital letters (A, B, C, D, ..)
Name? 1 a: small letters (a, b, c, d, ...)
Quality Lab 1 1: numbers (0, 1, 2, 3, 4, ...)
9.4 Serial/Version number (Setup 3)

This option is used to identify the software version and the serial number of your instrument
without having switch off the instrument.
- Press the <SETUP> key.

Select "3.Serial/Version" .
Setup(0-7)? 3
Serial/Version
Press the <ENTER> key. The serial number of your
Serial :xxxx xxxx instrument as well as the software version are displayed.
Version: x.xxx
9.5 LCD Contrast (Setup 4)

This option is used to setup the contrast of the LCD display in your instrument.
Setup(0-7)? 4 Select "4.LCD Contrast".

LCD Contrast Confirm with <ENTER>.
LCD Contrast? 4 Press the corresponding number key on the alphanumer-

Low:0 <-> 6:High ical keyboard (0:low, 3:normal, 6:high)

Setup
9.6 International (Setup 5)

This option is used to select the date format and the unit for the temperature (C or F).
Setup(0-7)? 5 Select "5.International".

International Confirm with <ENTER>.
International?
Select "Date" or "Temp.Unit" with the key.
Date Confirm with <ENTER>.
9.6.1 Setup date format

Select "Date" with the key. Confirm with <ENTER>.
International?
Date
Select your date format with the key.
Date? YYYY: Year
YYYY/MM/DD MM : Month
DD : Day
9.6.2 Setup temperature unit

Select "Temp.Unit" with the key. Confirm with <EN-
International?
TER>.
Temp.Unit
Select your temperature unit with the key.
Temp.Unit?
C C : degree Celsius
F : degree Fahrenheit
9.7 Lock with password (Setup 6)

Select "6.Lock".
Setup(0-7)? 6
Lock
Select "On" with the key.
Lock?
On

Setup
Password? Enter your password. Make sure to keep it in a safe place

in case you would forget it.
********
Enter your password a second time to confirm the correct
Password? spelling.
********
In case you would have forgotten your password you will need to call the METTLER
TOLEDO service.
9.8 Beep (Setup 7)

Setup(0-7)? 7 Select "7.Beep".

Beep Confirm with <ENTER>.
Beep? Select "On" with the key.

On Confirm with <ENTER>.
Enter your beep selection:

Beep Type(1-4)?
1: long and fast
4
2: long and slow
3: short and fast
4: short and slow

Error messages and malfunctions
10. Error messages and malfunctions
Messages appear in the display for several errors and malfunctions; causes and corrective
measures for these and other faults are listed below.
Error message Cause Measure
Prism not clean You have not cleaned the prism First clean the prism and then
for a zero point calibration calibrate
No sample You have no sample in the First add the sample

measuring cell and have
pressed the Meas. key
Busy ! Key is locked! You have, e.g. (Press Reset, if you wish to
pressed the Method key during abort the measurement)
a measurement
Protected ! The method is protected! You Remove protection as appropri-
have pressed the Meas. Para., ate (see Section 9.7)
Calcu. Para. or Print Para. key
Out of range Value below or above measure-

ment range for this sample
Temp Error The temperature sensor of the Switch the instrument off and
thermostat control is faulty call METTLER TOLEDO service
Lamp NG The LED light source is faulty Switch the instrument off and
call METTLER TOLEDO service
Malfunction Possible cause Measure
Instrument can not Cable not plugged in properly, Check power cable, on/off switch
be switched on fuse blown and fuses
Stored data Internal battery dead Switch the instrument off and
have been lost call METTLER TOLEDO service
(e.g. date)
Measurements of Prism faulty Switch the instrument off and

same sample not call METTLER TOLEDO service
reproducible
Blank display Display contrast is set to mini- Change contrast setting (see
mum or maximum Section 9.5)

Standard and optional equipment
11. Standard and optional equipment
11.1 Standard equipment

Order No.
One BRIX Refractometer RE20B

One set of Operating Instructions 51709840
One power cable in accordance with your order
11.2 Optional equipment
GA42 printer:
- Alphanumerical printer (serial) GA42
- Set of paper rolls (5 pcs) 00072456
- Ribbon catridge (black) 00065975
- Connection cable (not included) 51190362
PC software (for Windows 95 & 98):

- Data transfer software to EXCEL (version 7.0 or later) 51324100
- Connection cable to computer 51190362

MemoCard RE20B (Short Instructions)
Sample Sample No, Sample ID Function Function selection

0. Data file
1. Set check
(name, value, tolerance)
Report Select print format 2. Periodic check
off (check interval)
short (show check list)
GLP 3. Periodic calibration
variable (calibration interval)
(show calibration list)
Method Method selection 4. Memory clear
0: Brix 5. Wait time
1: HFCS42 6. Decimals
2: HFCS55
Setup Setup selection
Display Display selection 0. Interface
(Printer, RS-232)
1. Date & Time
Print Print message or parameter 2. Name
3. Serial/Version No.
4. LCD contrast
Measure Start measurement 5. International
(date format)
(C or F)
BS Backspace (erase) 6. Lock
7. Beep
Clear Clear entry (2x to clear name)

Check Run instrument check (*)
Periodic check (on check date)
Esc. Escape Daily check (all other dates)
Calib. Perform an adjustment

Reset Return to main display
(*) Note: Once the periodic check has been performed the CHECK key will automatically switch to the daily
check. In order to perform a periodic check again, set check interval (Function 2) to 0 Day to force
a periodic check on today's date. This will especially be required if the periodic check has failed, in
order to perform it again.

Table
Annex 1: BRIX Table
The stored conversion tables in the instrument are based on: Adjusted refractometer values
for the concentration of sucrose in water at 20 C ( = 589.3 nm). Based on the 16th session
of the International Commission of Uniform Methods for Sugar Analysis (ICUMSA), 1974.
The temperature correction tables used in the instrument are based on the International
Recommendation OIML R 108 (Edition 1993, E). See Annex 2.
BRIX table
Brix Refractive Brix Refractive Brix Refractive

% Index nD % Index nD % Index nD
0 1.33299 30 1.38115 60 1.44193
1 1.33442 31 1.38296 61 1.44420
2 1.33586 32 1.38478 62 1.44650
3 1.33732 33 1.38661 63 1.44881
4 1.33879 34 1.38846 64 1.45113
5 1.34026 35 1.39032 65 1.45348
6 1.34175 36 1.39220 66 1.45584
7 1.34325 37 1.39409 67 1.45822
8 1.34476 38 1.39600 68 1.46061
9 1.34629 39 1.39792 69 1.46303
10 1.34782 40 1.39986 70 1.46546
11 1.34937 41 1.40181 71 1.46792
12 1.35093 42 1.40378 72 1.47037
13 1.35250 43 1.40576 73 1.47285
14 1.35408 44 1.40776 74 1.47535
15 1.35568 45 1.40978 75 1.47787
16 1.35729 46 1.41181 76 1.48040
17 1.35891 47 1.41385 77 1.48295
18 1.36054 48 1.41592 78 1.48552
19 1.36218 49 1.41799 79 1.48810
20 1.36384 50 1.42009 80 1.49071
21 1.36551 51 1.42220 81 1.49333
22 1.36720 52 1.42432 82 1.49597
23 1.36889 53 1.42647 83 1.49862
24 1.37060 54 1.42862 84 1.50129
25 1.37233 55 1.43080 85 1.50398
26 1.37406 56 1.43299
27 1.37582 57 1.43520
28 1.37758 58 1.43743
29 1.37936 59 1.43967

Table
Annex 2: Temperature corrections for BRIX refractometer values
BRIX
Temp C 0 5 10 15 20 25 30 35 40
to be substracted from the BRIX value
10.00 0.52 0.56 0.59 0.61 0.64 0.67 0.69 0.71 0.72
11.00 0.48 0.51 0.54 0.55 0.58 0.61 0.63 0.65 0.65
12.00 0.44 0.47 0.49 0.50 0.52 0.55 0.57 0.58 0.58
13.00 0.39 0.42 0.43 0.44 0.46 0.49 0.50 0.51 0.51
14.00 0.35 0.37 0.38 0.39 0.40 0.42 0.43 0.44 0.44
15.00 0.29 0.31 0.32 0.33 0.34 0.35 0.36 0.37 0.37
16.00 0.24 0.25 0.26 0.27 0.28 0.28 0.29 0.30 0.30
17.00 0.18 0.19 0.20 0.20 0.21 0.21 0.22 0.22 0.23
18.00 0.12 0.13 0.13 0.14 0.14 0.14 0.15 0.15 0.15
19.00 0.06 0.06 0.07 0.07 0.07 0.07 0.07 0.08 0.08
BRIX
Temp C 0 5 10 15 20 25 30 35 40
to be added to the BRIX value
21.00 0.06 0.07 0.07 0.07 0.07 0.07 0.08 0.08 0.08
22.00 0.13 0.14 0.14 0.14 0.14 0.15 0.15 0.16 0.16
23.00 0.20 0.21 0.21 0.22 0.22 0.23 0.23 0.23 0.23
24.00 0.27 0.28 0.29 0.29 0.30 0.30 0.31 0.31 0.31
25.00 0.34 0.35 0.36 0.37 0.38 0.38 0.39 0.39 0.40
26.00 0.42 0.43 0.44 0.45 0.46 0.46 0.47 0.47 0.48
27.00 0.50 0.51 0.52 0.53 0.54 0.55 0.55 0.56 0.56
28.00 0.58 0.59 0.60 0.61 0.62 0.63 0.64 0.64 0.64
29.00 0.66 0.67 0.68 0.69 0.70 0.71 0.72 0.73 0.73
30.00 0.74 0.75 0.77 0.78 0.79 0.80 0.81 0.81 0.81

Table
BRIX
Temp C 45 50 55 60 65 70 75 80 85
to be substracted from the BRIX value
10.00 0.74 0.74 0.74 0.75 0.76 0.77 - - -

11.00 0.67 0.67 0.67 0.68 0.68 0.69 - - -
12.00 0.60 0.60 0.60 0.60 0.61 0.61 - - -
13.00 0.53 0.53 0.53 0.53 0.53 0.53 - - -
14.00 0.45 0.45 0.45 0.45 0.45 0.46 - - -
15.00 0.38 0.38 0.38 0.38 0.38 0.38 0.38 0.37 0.37
16.00 0.30 0.31 0.31 0.31 0.31 0.31 0.30 0.30 0.30
17.00 0.23 0.23 0.23 0.23 0.23 0.23 0.23 0.23 0.22
18.00 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15
19.00 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08
BRIX
Temp C 45 50 55 60 65 70 75 80 85
to be added to the BRIX value
21.00 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.07
22.00 0.16 0.16 0.16 0.16 0.16 0.15 0.15 0.15 0.15
23.00 0.24 0.24 0.24 0.24 0.23 0.23 0.23 0.23 0.22
24.00 0.32 0.32 0.32 0.32 0.31 0.31 0.31 0.30 0.30
25.00 0.40 0.40 0.40 0.40 0.39 0.39 0.39 0.38 0.37
26.00 0.48 0.48 0.48 0.48 0.47 0.47 0.46 0.46 0.45
27.00 0.56 0.56 0.56 0.56 0.55 0.55 0.54 0.53 0.52
28.00 0.65 0.65 0.64 0.64 0.64 0.63 0.62 0.61 0.60
29.00 0.73 0.73 0.73 0.72 0.72 0.71 0.70 0.69 0.68
30.00 0.82 0.81 0.81 0.81 0.80 0.79 0.78 0.77 0.75

Table
Specifications
Type and model name RE20B BRIX Refractometer

Measuring method optical detection of critical angle with Na-D line (589.3 nm)
Measuring range BRIX 0.00 to 100.00 %
Precision BRIX 0.03 %
Repeatability BRIX 0.01 %
Resolution BRIX 0.01 %
Temperature range T 15 to 40 C
Temperature precision T 0.05 C
Temperature compensation range
BRIX 15 to 40 C
Sample required min. 0.2 mL, 0.4 mL to cover the ring mark
Typical measuring time ~ 5 sec (depending on sample)
Display LCD (Liquid crystal display), 2 line display
Ambient condition 1) Temperature: 5 ~ 35 C
2) Humidity: below 85 % RH (no condensation)
Power source AC 100-240 V, 50/60 Hz
Power consumption 20 W
Dimension 270 mm (W) x 400 mm (D) x 225 mm (H)
Weight 6 kg

FCC Rules and Radio Interference Regulations
This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to
both Part 15 of the FCC Rules and the radio interference regulations of the Canadian Department of
Communications. These limits are designed to provide reasonable protection against harmful interference
when the equipment is operated in a commercial environment. This equipment generates, uses and can
radiate radio frequency energy and, if not installed and used in accordance with the instruction manual, may
cause harmful interference to radio communications. Operation of this equipment in a residential area is like to
cause harmful interference in which case the user will be required to correct the interference at his own
expense.
To protect your METTLER TOLEDO products future:
METTLER TOLEDO Service assures the quality, measuring accuracy and
preservation of value of all METTLER TOLEDO products for years to come.
Please send for full details about our attractive terms of service.
Thank you.
Printed on 100% chlorine-free paper, for the sake of our environment.
Subject to technical changes and to the availability

*P51709840* of the accessories supplied with the instruments.
Mettler-Toledo GmbH 2000 ME-51709840 Printed in Switzerland 00/01/2.12
Mettler-Toledo GmbH, Analytical, Sonnenbergstrasse 74, CH-8603 Schwerzenbach, Tel. (01) 806 77 11, Fax (01) 806 73 50,
Internet: http://www.mt.com

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RE20B BRIX Refractometer

2. Safety measures .............................................................................................. 6

3. BRIX, HFCS and Refractive Index .................................................................. 7

4. The instrument .............................................................................................. 10

5. Getting started ............................................................................................... 16

6. Sample (Number and ID) .............................................................................. 20

8. Functions (password protected) ................................................................. 21

METTLER TOLEDO RE20B BRIX Refractometer 3

9. Setup (password protected) ......................................................................... 29

10. Error messages and malfunctions .............................................................. 34

11. Standard and optional equipment ............................................................... 35

MemoCard RE20B (Short Instructions) ........................................................................ 36

Annex 1: BRIX Table ...................................................................................................... 37

Annex 2: Temperature corrections for BRIX refractometer values ......................... 38

4 METTLER TOLEDO RE20B BRIX Refractometer

METTLER TOLEDO RE20B BRIX Refractometer 5

Measures for your protection

Do not work in a hazardous area! The housing of the refractometer is not

Measures for operational safety

6 METTLER TOLEDO RE20B BRIX Refractometer

3. BRIX, HFCS and Refractive Index

3.1 Definition of refractive index

If a ray of light at a particular angle passes

Air If a ray of light passes into an optically less

METTLER TOLEDO RE20B BRIX Refractometer 7

3.2 Definition of BRIX

3.3 Definition of HFCS42 and HFCS55 (Inverted sugar)

Inverted sugar concentration denominates the concentration in weight % of an isomerized

8 METTLER TOLEDO RE20B BRIX Refractometer

3.4 Measurement principle

The light emitted by the RE20B pass-

Setup of the measurement system

METTLER TOLEDO RE20B BRIX Refractometer 9

10 METTLER TOLEDO RE20B BRIX Refractometer

4.1 General overview

9. AC power connector (IN)

METTLER TOLEDO RE20B BRIX Refractometer 11

7 8 9 Check Calib. BS Method

. 0 - Clear Esc. Reset Measure

Description of each key and its function:

Report Report Key to change print format.

Method Method Key to change Method.

Print Print Key to print the displayed message or parameters

12 METTLER TOLEDO RE20B BRIX Refractometer

Function Function Key to selectet following functions:

Setup Setup Key to select the setup parameters as follows:

Measure Key to start a measurement

METTLER TOLEDO RE20B BRIX Refractometer 13

. Dot and hyphen key

0 Ready After a few seconds the main display will appear:

14 METTLER TOLEDO RE20B BRIX Refractometer

4.4 Measures for correct measurements

METTLER TOLEDO RE20B BRIX Refractometer 15

5.1 Setting up the instrument

Press the <SETUP> key

Interface? Select "0.Interface". Confirm with <ENTER>.

Printer? Select "GA" with the key. Confirm with <ENTER>.

5.1.2 Date and time (Setup 1)

Press the <SETUP> key

Setup(0-7)? 1 Select "1.Date&Time". Confirm with <ENTER>.

Enter date (the date format is selected in the menu Setup

16 METTLER TOLEDO RE20B BRIX Refractometer

5.1.3 Enter the lab name (Setup 2)

5.2 Performing the first adjustment (calibration)