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Article history:
Received 1 April 2017 As early as 2004, the Fingerprint Working Group (EFP-WG) of the European Network of Forensic Science
Received in revised form 31 August 2017 Institutes (ENFSI) has organised prociency tests (PTs), as well as collaborative exercises (CEs), as a way
Accepted 4 September 2017 of raising standards within the ngerprint profession.
Available online xxx This article provides an overview of the three collaborative exercises carried out in 2015. The
characteristics of the testing programme are summarised, followed by an overview of the knowledge that
Keywords: has been gained, including depicting what lessons have been learnt.
Fingermarks Crown Copyright 2017 Published by Elsevier Ireland Ltd. All rights reserved.
ENFSI EFP-WG
Collaborative exercises 2015
Visualisation
Imaging
Individualisation
https://doi.org/10.1016/j.forsciint.2017.09.002
0379-0738/Crown Copyright 2017 Published by Elsevier Ireland Ltd. All rights reserved.
56 A. Mattei et al. / Forensic Science International 280 (2017) 5563
3. Test overviews
Two pieces of white paper; each piece should have been treated of the visualised dots; all samples were photographed on the same
with a different amino-acid reagent, i.e. for example one with day.
ninhydrin and the other with 1,8-diazauoren-9-one. One dot resulted in a score of 1, two dots in a score of 2 and all
A brown piece of paper; in this case the participant was free to three dots in a score of 3.
choose which visualisation technique(s) should be used (Fig. 1).
3.2. Imaging Collaborative Exercise (Im-CE)
To achieve uniform test samples for every participant, dots of
three different amino acid solutions were placed in known The participants were asked to enhance four photographs
volumes and concentrations. In addition, a control sample of containing ngermarks (Fig. 3) in order to evaluate their capability
water was also included (Fig. 2). The amino acids present in the in providing extra information (I, II and III details) which could
solution were glycine, alanine, aspartic acid, threonine, histidine, assist a practitioner in his/her decision-making process. In doing so
valine, leucine, isoleucine, ornithine and glutamic acid. These it should be also important to avoid processing techniques that
amino acids were selected as they are commonly present in introduce permanent changes or artifacts within the image.
ngerprint residue. Ultimately the dots contained the amino acids The selected images were reproduced by NFI to resemble
listed each one at the concentration reported in Fig. 2 for the total typical casework ngermarks using similar surfaces and known
volume indicated. donors (true source):
The samples were prepared between the 12th and 16th of June
2015. To ensure homogeneity of the test materials, batch sampling 1. Tin can treated with cyanoacrylate
was undertaken after every fth sample, these were treated within 2. Black cardboard treated with indandione
the NFI to demonstrate the presence of amino acids. The remaining 3. White paper with blue text treated with ninhydrin
samples were packaged and sent to participants on the 17th of June 4. Brown wrapping paper treated with ninhydrin
2015.
Post treatment, samples and a detailed response form were Participants were asked to record the software programs and
returned to the NFI, where two individuals assessed the presence image processing techniques that were used to enhance the
images.
On the 17th of June 2015 the test material was made available
for registered participants by three download links (RAW format
le, PSD format le and TIFF format le) to the Imaging exercise on
Google Drive. Response forms were sent individually to all
participating institutes.
Fig. 4. Examples of ngermarks (n. 2, 4 and 7) used for the Individualisation test.
58 A. Mattei et al. / Forensic Science International 280 (2017) 5563
Sample 1 white paper (Table 2): 52% of the participants, 4.3.1. Quality of the mark
treating the sample with a single amino acid reagent, were able to On the response form, the participants were asked to give an
visualise the maximum of three dots. evaluation of the quality of the mark based on clearness of the
Sample 2 white paper (Table 3): 28% of the participants, ridges, amount of distortion, etc. The participants had to write
treating the sample with a single amino acid reagent, were able to down a (G) for good, (M) for moderate or (B) for bad quality. Fig. 6
visualise the maximum of three dots. provides a general overview.
Sample 3 no conclusive analysis is possible.
4.3.2. Total number of assigned minutiae
4.2. Imaging Collaborative Exercise (Im-CE) Within the individualisation process, often a method is applied
called the ACE-V method. The rst stage of the method is
From a total of 46 subscribers, 33 participants returned their commonly known as the analysis phase. In the analysis phase,
results (71%). an encoding is made (usually done individually by a latent print
All results were made available in pdf format on Google Drive examiner) of all minutiae that are considered present in the mark.
through a download link. On the response form, a specic question was asked to all
To enhance the photos ve different programs were used plus a participants to write down the total number of minutiae found in
common lter: the analysis phase.
In Fig. 7, the observed minutiae count is presented on the
Photoshop (Cs2-Cs6) (Raw converter included) > 77,2% vertical axis (040). The marks are presented on the horizontal
DCS4 (Foster + Freeman) > 17% axis (mark #01#10). The results are presented using boxplots
Table 1
Result sheet for the Visualisation test.
Lab code Paper 1 Reagent Paper 2 Reagent Paper 3 Reagent Lab Code Paper 1 Reagent Paper 2 Reagent Paper 3 Reagent
FP201502 3 IND 2 NIN 0 Sequence FP201528 3 DFO 2 NIN 2 Sequence
FP201503 2 NIN 3 IND 0 Sequence FP201530 3 NIN 2 IND 0 IND
FP201504 2 IND/NIN 3 IND/NIN 0 Sequence FP201531 3 DFO/NIN 3 NIN 2 NIN
FP201506 3 DFO 3 NIN 1 Sequence FP201532 3 IND/NIN 3 IND/NIN 0 Sequence
FP201507 2 DFO 3 NIN 0 Sequence FP201534 3 DFO 2 NIN 0 Sequence
FP201508 3 NIN 3 IND 1 Sequence FP201535 2 DFO 3 NIN 2 Sequence
FP201509 2 DFO/IND/NIN 2 DFO/IND/NIN 0 Sequence FP201536 3 IND 2 NIN 1 Sequence
FP201510 3 IND/NIN 3 IND/NIN 0 Sequence FP201537 2 IND 2 NIN 3 Sequence
FP201511 3 IND/NIN 3 IND/NIN 0 Sequence FP201539 2 DFO 2 NIN 1 Sequence
FP201512 1 NIN 0 DFO 2 NIN FP201540 0 IND 2 NIN 0 Sequence
FP201515 2 DFO 2 NIN 0 Sequence FP201541 2 DFO 1 IND 0 DFO
FP201516 0 NIN 0 NIN 2 NIN FP201543 3 IND/NIN 2 NIN 0 Sequence
FP201517 0 DFO 2 NIN 0 Sequence FP201548 0 DFO/NIN 1 DFO/NIN 0 Sequence
FP201518 3 IND 2 NIN 0 IND FP201550 3 DFO/NIN 3 DFO/NIN 0 Sequence
FP201520 3 DFO 0 NIN 0 Sequence FP201552 2 IND/NIN 2 IND/NIN 1 Sequence
FP201521 3 DFO 2 NIN 0 Sequence FP201553 3 IND 0 NIN 0 IND
FP201522 3 NIN 3 DFO/NIN 3 Sequence FP201555 0 IND/NIN 0 IND/NIN 0 Sequence
FP201524 3 IND 2 NIN 0 Sequence FP201557 3 DFO/IND/NIN 3 DFO/IND/NIN 1 Sequence
FP201525 1 IND/NIN 0 ORO 1 Sequence FP201559 1 NIN 3 IND 0 Sequence
FP201527 3 DFO 3 NIN 0 Sequence FP201562 0 NIN 0 NIN 0 Sequence
Table 2 Table 3
Sample 1, white paper. Sample 2, white paper.
covering the total of observed minutiae per mark, from the lowest the orange box represents 50% of the observed minutiae count:
to the highest observed minutiae count. Each boxplot contains a 50% of the respondents observed a minutiae count between
vertical line and a box, the vertical line represents all observed 14 and 19;
minutiae counts, the box represents 50% of all observed minutiae the lowest minutiae count for mark #01 is 9 minutiae;
counts. the highest minutiae count for mark #01 is 28 minutiae;
Example for Mark #01: the average minutiae count for mark #01 is 16 minutiae (this is
represented by the horizontal black line in the box).
Table 4
Table displaying the number of false negative results (22) and false positive results
(6) of the individualisation part.
M1 M2 M3 M4 M5 M6 M7 M8 M9 M10
False negatives 0 3 0 3 0 2 0 11 3 0
False positives 1 0 0 0 0 0 5 0 0 0
5. Discussion
Fig. 8. Summary of the results for the individualisation part of the exercise. For most of the participants the instructions did not provide
sufcient clarity to undertake the test. This is borne from the fact
that 30% of the laboratories undertook sequential treatment,
4.3.3. Individualisation part rather than the intended single visualisation technique. The
A summary of results for the 50 participants of the Individu- unintended consequence was that an inter-laboratory compari-
alisation part of the Collaborative exercise 2015 is shown in Fig. 8. son of only twenty-four participants out of a possible forty
15 out of the 50 participating institutes marked all answers participants was possible.
correctly (100% score). One institute reported back 1 correct According to recent literature [5,6], the composition of amino
answer (10% score). acids within test solutions should be representative of natural
The Individualisation part of the collaborative exercise secretions; therefore, the absence of serine, an abundant amino
contained a total of 9 matches and 1 no match (Mark acid in sweat, is considered a factor that might inuence test
#07 was the only no match). The best results were obtained results.
with Mark #01, in which only 1 incorrect result was given (98%
correct). The second best results were obtained with Mark #05, That being stated, in Figs. 10 and 11 it is possible to observe the
in which 2 of the answers were marked as an incorrect result performance of ninhydrin (NIN), 1,8-diazauoren-9-one (DFO) and
(96% correct). As expected Marks #07, #08 and #09 were the 1,2-indandione (IND). For sample 1, indandione seems to be a more
most difcult marks. The most difcult mark was Mark #07, in effective technique.
which 27 of the answers were marked incorrect (46% correct), For sample 2, it can be seen that the participants with the
followed by Mark #08, in which 17 answers were marked maximum score used the visualisation techniques, indandione or
incorrect (66% correct) and Mark #09, in which 20 answers were ninhydrin.
marked incorrect (60% correct) (Fig. 9). Unfortunately, the lack of details around the solutions
Of the incorrect results, a distinction is made between false employed prevents the authors from making an in-depth
negative results (the true donor in the reference set was incorrectly evaluation around furthering an understanding on whether the
excluded) and false positive results (the mark was attributed to a observed results correlate to the reagents components.
wrong donor) (Table 4). Note: the false positive answer for Mark However, if we consider sample #1 and sample #2 as consistent
#01 was considered an administrative error and is not considered samples, two main observations can be made:
as a false attribution.
Fig. 9. Overview of correct/incorrect answers for each mark for the individualisation part of the exercise.
A. Mattei et al. / Forensic Science International 280 (2017) 5563 61
Due to the design limitation of the 2015 Im-CE, the provider was
not in a position to report these ndings, including the expected
results, to the participants.
As a lesson learnt, it is acknowledged that one of the most
important objectives of CEs is the sharing of information with
participants in order to promote professional development and
best practice. ENFSI EFP-WG should therefore ensure that this
aspect is taken into account for all future CE provision.
Table 6
Individualisation tests 2015: in-depth overview of mark #07 and mark #08.
overview of the factors that could have negatively inuenced the Construction of the test: with regard to the brown paper, as a
comparison of the two marks is given in Table 6. result of the lack of the recording of the outcome of each step in the
sequential treatment, no conclusive analysis of the results was
5.4. Issues and lessons learnt possible.
Response form: in order to support the evaluation of the test, the
The purpose of the pilot study is to undertake an indepen- response form should be designed to collect more detailed
dent evaluation of the test in order to identify ambiguity information from the participants. For example, having the
regarding the instructions, to verify that the test materials are t information about formulations or methods, can allow for
for purpose and to ensure that every element from the receipt of unexpected results to be further investigated as part of root cause
the material to the return of the response form can be accounted analysis.
for. The risk of not conducting the pilot study has the potential Reporting of results: the report to participants must be supplied
to invalidate the test results, or to reduce the ability of the within a reasonable time-frame and with sufcient details, so that
provider to analyse results. This applies across all of the areas the participants can evaluate their individual results against the
mentioned in this paper (visualisation/imaging/individualisa- overall test results and have the opportunity to make improve-
tion). ments so that casework samples are not unduly affected.
In addition to the pilot study it is essential to conduct quality
checks on the samples that are produced for the test. 5.4.2. Imaging Collaborative Exercise (Im-CE)
The importance of conducting both the pilot study and the Construction of the test: even if the images submitted for the
quality checks of the samples cannot be underestimated. analysis were of the appropriate le format and they were in line
with the average casework images, in order to maximize the
5.4.1. Visualisation Collaborative Exercise (V-CE) usefulness of the test the provider should include in the report to
Instructions of the CE: the instructions relating to the treatment the participants the procedure which has been taken as a reference
of the two pieces of white paper were ambiguous, resulting in 14 of when designing the test (e.g. what anticipated results were
the 40 laboratories performing sequential treatments on the item. expected).
These results were excluded from the evaluation and therefore Reporting of results: although the individual response forms
invalidated this part of the test for those laboratories. collated all the procedures followed by the participants, this level
In addition, for practical purposes the amino acid solutions of detail was not published in the report. The report to participants
were placed as dots and not as friction ridge details. This was not must be supplied within a reasonable time-frame and with
mentioned in the instructions, therefore when the dots were sufcient details, so that the participants can evaluate their
developed the participants did not recognize these as a result individual results against the overall results and have the
because they were expecting friction ridge details. Although the opportunity to make improvements.
use of friction ridge details should not be regarded as mandatory,
there is a need for this to be made clear in the instructions as this is 5.4.3. Individualisation Collaborative Exercise (In-CE)
a deviation to the outcomes that would be anticipated in the Construction of the test: with regard to the total number of
working environment that is being tested. minutiae assigned, it would be better to ask for the images in
Consistency of samples: on the white paper using the same order to see how the participant marks up the minutiae. This is a
technique some participants obtained different results, concluding critical point as reported in Ref. [11] and it needs to be addressed in
that the samples were not homogeneous. Every effort must be a CE in the near future.
taken in the preparation of the samples in order to ensure In addition, a scale must be included and effort made to ensure
homogeneity; otherwise the test results could be invalidated. that images are in focus.
A. Mattei et al. / Forensic Science International 280 (2017) 5563 63
This project has been funded with the support of the European
Commission. This publication reects the views only of the