Professional Documents
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Bioethanol Production
from
Municipal Solid Waste
Department of Civil and Environmental Engineering
Aiduan Li
Dr Majeda Khraisheh
Dr Blanca Antizar
Background
Previous studies
Ongoing project
Waste characterization
Experiments & results
Conclusions
Future work
Background: MSW - rubbish or resources?
Average Biodegradability Overall
Composition of MSW % Wt Fraction Biodegradability
in MSW 1 (%) 1 (%) 1
Paper and card 27.8 100 27.8
Organics 34.3 100 34.3
Fines (< 10 mm) 1.3 60 0.8
Textiles
4.4 Million tonnes MSW produced 2.4 50
in London in 20031.2
Miscellaneous combustible 10.3 50 5.2
Glass 7.5 0.0 0.0
Other non-combustibles 1.6 0.0 0.0
Plastic film 5.0 0.0 0.0
Ferrous metal 3% rising2.9every year 0.0 0.0
Non ferrous metal 0.9 0.0 0.0
Waste electrical and electronic
equipment (WEEE) 0.3 0.0 0.0
Household hazardous waste
(HHW) 0.2 0.0 0.0
Dense plastic 5.5 0.0 0.0
Total 100.0 - 69.3%
Source: Burnley S J, Coleman T and Gronow J R (1999) The Impact of the Landfill Directive on
Strategic Waste Management in the UK, Sardinia 1999 International Conference on Landfill.
1 Dry matter basis
Background: MSW as Feedstock?
Source: Defra, UK
Background: Ethanol Market
Source: Berg, C. (2004). World fuel ethanol analysis and outlook. F.O. Licht, Commodity Analysts.
[URL: http://www.distill.com/]
Background: Ethanol process challenges
Improving technology
to reduce cost:
Improved efficient
pretreatment
Multi-substrates
Investigation of mixed waste, by combining construction lumber
waste, almond tree prunings, wheat straw, office waste paper,
and newsprint, with pretreatment method of dilute-acid hydrolysis2,
reported that 80-90% theoretic of glucose yield can be obtained
with enzyme loading of 66 FPU after 100 hours hydrolysis.
Sources:
1
Kim, S. B. & Moon, N.K. (2003). Enzymatic digestibility of used newspaper treated with aqueous ammonia-hydrogen peroxide
solution. Applied Biochemistry and Biotechnology. Vol. 105-108. pp. 365-373.
2
Nguyen, Q. A., Keller, F.A., Tucker, M.P., Lombard, C.K., Jenkins, B. M, Yomogida, D. E., and Tiangco, V.M. (1999).
Bioconversion of mixed solids waste to ethanol. Applied Biochemistry and Biotechnology. Vol. 77-79. pp. 455-472.
On-going Project
Enzyme
and Yeast SSF
MSW /Fermentation Ethanol
Pre-processing Yeast
Process Simulation
Enzyme
Current Stage
Waste characterization
100
80
60
40
20
0
s s r r
g g ss e e se
elin elin r a
pap pap lu
l o
G l
pe pe s
ap e
o ot ew r C
tat ar
r N S
c
o C
P
Sample
C H N Analysis
50
40
% content
% Carbon
30
% Hydrogen
20
10 % Nitrogen
0
ge
r
ss
g
g
er
pe
in
in
ra
ra
ap
el
el
Pa
e
G
Pe
Pe
sp
Av
p
ew
ra
t
to
ro
Sc
ta
N
ar
Po
Sample
80
carrot peelings
60 Potato peelings
40 Grass
20 Newspaper
0 Scrap paper
E
E
E
W
t
en
S
+S
M
l+
4+
m
4+
O3
HC
SO
at
SO
HN
re
H2
et
H2
pr
no
Pretreatment methods
Cellulase Effect
Glucose yield (%
100
Theoretic)
80
60 T.reesei
40
20 T.viride
0
gs gs ass p er er
il n i n r a p
ee eel G sp pa
tp o
p e w rap
rr
o
ta
t N Sc
Ca o
P
Substrates
Hydrolysis condition: temperature 50oC, ph 4.8, enzyme loading 60 FPU, time 96 hours
Current Results: Cellulase effects (with
pretreatment)
Effects of Cellulase
Glucose yield ( % Theoretic)
120
100
80 T.reesei
60
40 T.viride
20
0
e l (C) (P) r) P) P
)
P P SP
c (G N (S N +
vi gs gs
s r
( r P+ C P+
A lin lin e
e e r as ape ap S r +N
t pe pe G sp
p
p
+G
ro tat
o ew cra +P
ar o N S C
C P
Pretreated Substrate
Hydrolysis condition: temperature 50oC, ph 4.8, enzyme loading 60 FPU, time 96 hours
Pretreatment: H2SO4 + SE
Current results: Glucose yield (after
pretreatment)
100 Avicel
Glucose yield (%
Potato peelings(P)
60
Grass (Gr)
40 New spaper (NP)
20 Scrap paper (SP)
0 SP+NP
C+P
0 24 48 72 96
C+P+Gr+NP+SP
Hydrolysis condition: temperature 50oC, ph 4.8, enzyme (T. viride) loading 40 FPU
Factorial experiment design
Sample: Carrot peelings Time: 72 hours, pH 4.8
Factors: A, Acid concentration: 1% and 4% Treatment: H2SO4 + SE
B, Temperature 121 and 134 0C Enzyme: T. viride
C, Enzyme loading: 10 and 60 FPU Hydrolysis temperature 50oC
2 134 1 60 72.50
3 134 4 60 61.16 B+ 58.62 49.72
- low
4 121 1 10 65.21 setting
B: Temperature
+
5 134 4 10 50.22 high 72.80 58.22 C+
g
in
setting
ad
6 134 1 10 56.70
lo
e
m
7 121 4 10 43.34
zy
En
B- 60.85 46.27 C-
8 121 1 60 68.45 A- A+
C:
A: Acid concentration
Factorial experiment design
Sample: Carrot peelings Time: 72 hours, pH 4.8
Factors: A, Acid concentration: 1% and 4% Treatment: H2SO4 + SE
B, Temperature 121 and 134 0C Enzyme: T. viride
C, Enzyme loading: 10 and 60 FPU Hydrolysis temperature 50oC
70
Glucose
50
X = A: Acid concentration
30 Y = B: Temperature 70.57 61.66
30 40 50 60 70 80 Z = C: Enzyme loading
AV, %
B: Temperature
+
B (Temperature) 0.04 0.13 high 72.80 58.22 C+
g
in
setting
ad
lo
C (Enzyme loading) 13.58 49.39
e
m
zy
AB (Acid 0.76 2.78
En
B- 60.85 46.27 C-
concentration*Temperature) A- A+
C:
A: Acid concentration
Conclusions
Pretreatment of dilute sulphuric acid hydrolysis followed with steam
explosion did increase in general the rate at which the maximum yield of
glucose was formed. However, this pretreatment did not give higher yields for
newspaper wastes.
Acknowledgement