four molecules of pyrrole linked by methyne bridges absorb visible light colors heme deep red Hemoglobin : a tetramer composed of two different types of subunits
The tetrameric structure of hemoglobin facilitates saturation with O2 in the lungs and release of O2 as it travels through capillary beds
B. OXYGEN DISSOCIATION CURVES
Myoglobin curve : hyperbolic Hemoglobin curve : sigmoidal (seperti huruf S) Interpretation When Po2 is high (in lungs) both myoglobin and hemoglobin saturated with O2 When Po2 is low (in oxygen-using tissues) hemoglobin cant bind oxygen as well as myoglobin myoglobin is a good oxygen-storage, hemoglobin is a good oxygen-vehicle C. OXYGENATION-DEOXYGENATION OF HEMOGLOBIN : CONFORMATIONAL CHANGES The binding of teh first O2 molecule to deoxyHb shifts the heme iron toward the plane of the heme ring from a position about 0.04 nm beyond it transmitted to the proximal (F8) histidine rupture of salt bridges between carboxyl terminal residues one pair of alpha/beta subunits rotates 15
The cooperativity in O2 binding in Hb comes from conformational changes in tertiary
structure that take place when O2 binds Conformational changes : changing from a T (tense) state with low affinity for O 2 to an R (relaxed) state with a high affinity for O2 D. HEMOGLOBIN TRANSPORTS CO2 AND PROTONS TO THE LUNGS After delivering O2 to the tissue Hb transport CO2 and protons to the lungs CO2 carries as carbamates carbamate changes the charge on amino terminals from positive to negative favoring salt bridges formation CO2 in venous blood : 15% as carbamate, remain as bicarbonate
The Bohr Effect : hemoglobins oxygen binding affinity is inversely related
to both acidity and to the concentration of carbon dioxide Protons for the Bohr effect arise from rupture of salt bridge
E. AGENTS THAT AFFECT OXYGEN BINDING
1. 2,3-Bisphosphoglycerate Formed in red blood cells Binds to HB in the central cavity formed by the four subunits increasing the energy required for the conformational changes that facilitate oxygen binding lowers the affinity of Hb for oxygen oxygen is readily bound when Hb contains 2,3-BPG 2. Proton Binding (Bohr Effect) Binding of protons lowers its affinity for oxygen pH of blood decrease as it enters the tissue (because CO2 produced by metabolism converted to carbonic acid) Dissociation of carbonic acid produce protons react with several amino acid residues in Hb conformational changes that promote the release of O2 In the lungs, proses diatas terjadi terbalik. Oxygen berikatan dengan Hb release protons combine with bicarbonate to form carbonic acid In tissues : pH of the blood is low because of the CO2 produced by metabolism O2 released from Hb In lungs : pH of the blood is higher because CO2 is being exhaled O2 binds to Hb 3. Carbon Dioxide Most of CO2 to the lungs as bicarbonate; some of it (15%) covalently bound to Hb as carbamate In the lungs : Po2 is high oxygen binds to Hb and CO2 released
Physical Organic Chemistry—Ii: Specially Invited Lectures Presented at the Second IUPAC Conference on Physical Organic Chemistry Held at Noordwijkerhout, Netherlands, 29 April–2 May 1974