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NEOSPORA CANINUM ELISA KIT
BIO K 218
For serum - Competition - Monowell

Neospora caninum is a protozoon that was original- Use of the kit

ly described as a parasite in dogs, in which it causes
myositis and encephalitis. Bovine neosporosis is now
recognised as a major cause of spontaneous abortion
in cattle. It is highly suspected on 20% of the farms
with repeated abortions and a cow that is seropositive
for Neospora caninum has a threefold greater risk of
aborting than a cow that is Neospora-negative. Neos-
pora is responsible for 21% of spontaneous abortions
occurring in an individual animal. This percentage rises
to 33% for the herd as a whole. Verticle transmission
is the rule (at least 80% of the calves born to seropo-
sitive cows are infected). Serotesting before the calfs
first colostrum intake will reveal prenatal infection.
The kit is designed to determine
cattles serological status for Neos-
pora caninum.
Reliable Results
The use of monoclonal antibody as
conjugate ensures excellent speci-
ficity and very reliable results.
The use of recombinant SRS2
Neospora caninum protein on the
plate also makes it possible to ob-
tain an excellent specificity

Ease-of-Use
Minimal hands-on-time
Results available in 160 minutes
for single or batch testing

Moab anti-SRS2
peroxidase label-
led

Serum sample

EIA Procedure

1- Microplate coated with rSRS2 Neospora caninum

purified P38 protein.
2- Add samples and positive and negative controls.
Incubate 2 hours at 37C+/-3C.
Wash
rSRS2 from Neos-
3- Add conjugate.
pora caninum
Incubate 1/2 hour at 37C+/-3C.
Wash
4- Add monocomponent TMB.
Wait 10 minutes
Add stop solution. Read at 450 nm

Bio-X Diagnostics
Site du complexe des Postes 49, rue Joseph Wauters BE-5580 Jemelle BELGIUM (Europe)
Tel: +32 (0)84.32.23.77 Fax: +32 (0)84.31.52.63 E-mail: info@biox.com http://www.biox.com
 www.biox.com
Kit performance
The performance of the BIO K 218 kit (Competitive test) was compared with BIO K 192
ELISA test (immunocapture test) on 270 blood serum samples. The results of these com-
parisons are shown in Graph 1. For immunocapture (Y axis) 1 corresponds to the value
obtained with the kits positive reference serum (S/P).

Graph 1

BIO K 218

- +
BIO K 192

- 236 5 241
+ 4 25 29
240 30 270

Concordance between the two tests: Kappa = 0.83

The concordance between the two tests is considered excellent.
Landis et Koch, The measurement of observer agreement for categorical data
Biometrics 1977, 33, 159-74

Bio-X Diagnostics
Site du complexe des Postes 49, rue Joseph Wauters BE-5580 Jemelle BELGIUM (Europe)
Tel: +32 (0)84.32.23.77 Fax: +32 (0)84.31.52.63 E-mail: info@biox.com http://www.biox.com
 www.biox.com
Combined results

- +
BIO K 218
- 27 5 32
+ 1 41 42
28 46 74

Seventy-four serum samples were taken on two farms, both of which had had a
large number of spontaneous abortions associated with Neospora caninum.
These 74 samples were tested using four commercial kits and one of the labora-
torys own kits. They were also evaluated by Western blot analysis. The results
were classified as positive or negative according to the majority of the kits outco-
mes. In the case of doubt, the Western blot was used as the reference.

Sensitivity: 89 %
Specificity: 96 %

Composition of the kit

BIO K 218 NEOSPORA CANINUM ELISA KIT

2 plates of 12 strips for a total of 192 tests

1 vial of positive reference serum
1 vial of negative reference serum
1 vial of conjugate ready to use
1 bottle of washing solution
1 bottle of monocomponent TMB
1 bottle of stopping solution

1 year of stability at 4C

Bio-X Diagnostics
Site du complexe des Postes 49, rue Joseph Wauters BE-5580 Jemelle BELGIUM (Europe)
Tel: +32 (0)84.32.23.77 Fax: +32 (0)84.31.52.63 E-mail: info@biox.com http://www.biox.com