Professional Documents
Culture Documents
The ABO Blood Group System glycosyltransferases that add sugars to a basic
precursor substance.
Routine ABO Testing
Forward grouping Importance of the H antigen
Reverse grouping 1. Production of A, B, and H antigens in fetal and
Routine reagents for ABO testing adult life
Requirements for routine ABO testing Interaction of Hh and ABO Genes
Inverse reciprocal relationship between the Immunodominant sugars confer blood group
forward and reverse type specificity
Naturally occurring antibodies <Insert Figure 64>
Postulated role of bacteria Importance of the H gene
ABO Antibodies Bombay phenotype
Predominantly IgM Importance of the A gene
Activate complement Importance of the B gene
Room temperature or colder <Insert figures 6-5 and 6-6>
Produce strong direct agglutination reactions Considerations for the AB blood group
Reagent anti-A
Reagent anti-B Molecular Genetics of ABO
Reagent anti-A,B Investigation of ABO alleles, epitope
Monoclonal antisera structures, and exons
Polyclonal antisera ABO gene located on chromosome 9 and
Considerations for routine blood bank consists of seven exons
testing Diversity at the ABO locus