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INTRODUCTION
METHODOLOGY
2. Using the microbiurette/ pin head as the dropper, the extract chloroplast is
dropped on the prepared chromatography paper (see Figure 1).
3. The extract is dropped at about 1.5 cm from the pointing end of the paper.
Then, the drop is dried with a hair dryer and the process is repeated for 3-4
times until one small dot of thick pigment available.
4. The paper strip is attached at the cork stopper using a pin. The strip is
placed vertically and straight into the test tube which contained solvent.
5. Let the solvent moves and the paper is removed before the solvent front
reaches the top of your chromatography paper.
7.3 𝑐𝑚
𝑅𝑓 = = 0.89 𝑐𝑚
8.2 𝑐𝑚
ii) Chlorophyll B (yellow green) pigment (purple spinach)
6.9 𝑐𝑚
𝑅𝑓 = = 0.86 𝑐𝑚
8.0 𝑐𝑚
iii) Anthocyanin (purple) pigment (purple spinach)
2.6 𝑐𝑚
𝑅𝑓 = = 0.33 𝑐𝑚
8.0 𝑐𝑚
DISCUSSION
CONCLUSION
REFERENCES
https://www.ukessays.com/essays/biology/characterization-of-photosynthetic-
pigments-using-paper-biology-essay.php
https://students.ga.desire2learn.com/d2l/lor/viewer/viewFile.d2lfile/1798/12844
/photosynthesis18.html