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I am keen to apply for this doctoral program as its research interests are an excellent
match for my academic background. My first research venture was as a MSc.
Dissertation student, I joined Prof. (Dr.) Rosamma Philip research group at Cochin
University of Science and Technology (CUSAT) for the MSc. dissertation project entitled
“Antimicrobial peptides derived from Sin Croaker”. The objective of this project was
focused on isolation of antimicrobial peptides from the Sin croaker, Johnius dussumieri
and determining its antimicrobial activity in vitro. In my dissertation, I have used
modified acetic acid-acetone method, Sep-pak ® c-18 cartridges (Waters, USA) and
Fast Protein Liquid Chromatography- FPLC
for the purification and Liquid growth
inhibition assay to determine antimicrobial activity.
I worked as an intern at National Centre for Antarctic & Ocean Research, Ministry of
Earth Sciences (India) for six months under the supervision of Dr. P.V Bhaskar. Here I
have undergone analysis on water samples from Indian Southern Ocean Expedition and
Equatorial samples. Analysis like Quantification of Exopolysaccharides (EPS) by phenol
sulphuric acid method, enumeration of microorganisms in samples by DAPI staining,
Identification of siderophore producing microorganisms by CAS assay, Isolation of DNA
from isolates producing good amount of EPS followed by PCR amplification.