ECE6008 Biosensors L T P J C

2 0 0 4 3
Version : 1.00
Pre-requisite: ECE5001
Course Objectives:
 To provide basic understanding of Biosensor working principle
 To offer thorough knowledge about the variety of sensing techniques for measurement and
detection of different analytes

Expected Outcomes:
The student will be able
 To gain the basic idea of biosensor and immobilization techniques.
 To differentiate between the types of biosensors available.
 To select a suitable biosensor for a given application.
 To apply the knowledge about the measuring different analytes to use them more effectively.

Student Learning Outcomes (SLO): 1,2,6,9,13 and 17

Module:1 Introduction to sensor and transducers 2 hours SLO:

1,2,9,17
Enzyme electrodes, Immobilization methods, entrapments of biomolecules, sensing element design
considerations

Module:2 Characteristics of Biosensors 4 hours SLO:

1,2,9,17
Electrochemical, optical, potentiometric, piezoelectric, voltametric, galvanometric CHEMFETs, ISFETs,
FET based Biosensors

Module:3 Amperometric Biosensor 4 hours SLO:

1,2,9,17
Amperometric enzyme electrodes: substrate and enzyme activity, Detection mode and transduction
method, mediated and modified electrodes, commercially available biosensor.

Module:4 Potentiometric Biosensor 4 hours SLO:

1,2,9,17
pH glass and ion selective electrodes, solid state and redox electrodes, gas electrodes, commercially
available biosensors

Module:5 Optical Biosensor 4 hours SLO:

1,2,9,17
fiber optic biosensor, Fluorophore and chromophore based biosensor, Bioluminescence and
chemiluminescence based biosensors

Module:6 Immunosensors 4 hours SLO:

1,2,9,17
Non labled and labled immunosensors, Microbial Biosensors: electrochemical, photomicrobial, Microbial
thermistor. Application of microbial biosensors in glucose, ammonia, acetic acid, alcohol, BOD, methane
sensing

Module:7 Hybrid Biosensor, In Vivo Biosensor and 6 hours SLO:

Commercial Biosensor 1,2,9,17
Hybrid biosensor, O2, Co2, blood gas, pH sensing, environmental monitoring, Glucose, Hcg, Glutamate,
cancer detection, and pollutant and environmental Biosensor
Module:8 Contemporary Issues 2 hours SLO:
2,17

Total Lecture: 30 hours

Reference Books:
1. Brian R Eggins, Chemical sensors and Biosensors, John Wiley Sons Ltd, 2004.
2. Loic J Blum and Coulet, “Biosensor: Principle and applications”, CRC Press, 2nd ed.2010
3. Janata, Jiri,"Principles of Chemical sensors", Springer, 2nd ed. 2009.
4. A. Turner “Biosensors: Fundamentals and applications” Oxford university press 1990
List of Projects for J component :
1. Clark electrode based biosensor for glucose measurement
2. pH measurement using potentiometric biosensor
3. Immobilization of biomolecules for sensing element
4. Air Quality Measurement using bioluminescence
5. Blood pH measurement using IR based optical biosensor
6. Immobilization of enzymes on electrodes surface for biomarkers

Date of Approval by the Academic Council 18.03.16