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Metaphase
The formation of spindle fibres is complete by the beginning of metaphase.
Spindle fibres attach to the kinetochores of the chromosomes. Chromosomes
line up along the equatorial plane of the cell.
The metaphase chromosomes orient themselves on the equatorial plate and
are clearly visible with a stain. Each metaphase chromosome is composed of two
sister chromatids attached to the spindle fibres of opposite poles. The chro-
matids are now ready to separate.
Anaphase
The unique feature of anaphase is chromatid separation. Chromatids sepa-
rate at their centromeres, and one group of chromosomes moves toward each
pole. The microtubules of the spindle shorten considerably, pulling the chromo-
somes towards the poles.
Telophase
The events of telophase are almost the reverse of those of prophase. This
phase begins as chromosome movement ceases. A new nuclear membrane is
formed at each pole which surrounds the daughter chromosomes. Each chro-
mosome begins to uncoil, becoming less condensed. Chromatin fibres are
formed and metabolic activities are initiated.
Finally, a nucleolus appears within each nucleus. When karyokinesis or divi-
sion of nuclear material is complete, the result is two identical nuclei.
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THE STRUCTURE OF EUKARYOTIC CELL
Vacuole Membrane bound sacks, 3-types Store materials, waste and water
Contractile
Found in fresh-water protists Removes excess water
Vacuole
Storage
Found in plant cells Stores toxic salts as crystals
Vacuole
Formed from the alternation of chloroplast, contains red, Gives variety of colour
Chromoplast
orange and yellow pigments
Large structure surrounded by double membrane, contains The control centre of the cell
Nucleus
chromosomes and nucleolus
Rod-like structure composed of DNA and protein, contains Controls all cellular activities
Chromosome
genes
Cell wall Found in plants, protista, fungi and some bacteria Protection and structural support
Cilia (9+2) Short projections extending from the surface of the cell Provides cellular movement
Flagella(9+2) Long projections extending from the surface of the cell Cellular locomotion
Cell organelles:
Nonmembranous granules composed of RNA and pro-
Ribosome tein, some attached to ER some free in cytoplasm some Synthesise protein
in chloroplast and mitochondria
Lipid synthesise
Smooth ER Lack ribosomes on outer surface
Drug detoxification
Double membranous organelles, inner membrane is Power house of the cell, produces
Mitochondria ATP energy
folded to form cristae
Found in animal cells, nonmebranous structures com- Help distribute chromosomes to new
Centrosome cells during cell division
posed of two centrioles.
Microbodies Membrane bound sacks containing variety of enzymes Destroys toxic chemicals such as
(Peroxysome) such as hydrogen peroxidase hydrogen peroxide