Professional Documents
Culture Documents
In Partial Fulfillment
Of the Requirements for the Course BIO 199
Undergradute Thesis
Globally, mangroves covered an estimated 137, 760 km² in 2001. Thirty-five percent of
the total 18 million ha of global mangrove forest are found in Southeast Asia (Honculada-
Primavera, 2000). And the Philippines holds at least 50% (Primavera et al., 2004) of the world’s
65 species of mangroves. The mangrove ecosystem is a crucial environment that shelters birds,
fish and invertebrates (Kathiresan and Bingham, 2001). They are responsible for coastal protection
(Sherman et al., 2001) and persist in a dynamic and physiological stressful environment that
activities as well as natural catastrophes such as storm surges and tropical storms, (Friess, D.,
2016) causing damages on agriculture, infrastructure and to the environment, including the
mangrove ecosystems. Due to the intensity of damage, sometimes entire mangrove forests are
destroyed, thus, losing its rich biodiversity. The strongest storm recorded in Philippines to make
landfall was Super Typhoon Haiyan (Yolanda) which sent blasts of up to 315–375 kph and storm
surges up to 7.4 m high in 2013, destroying vast mangrove community. But rehabilitation projects
In the rage of mangrove rehabilitation, the Ecosystem Research and Development Bureau
(ERDB) has pressed companies and organized groups to tie up with them on pest management-
driven mangrove farming. Growing mangrove community can recurrently experience disturbances
such as pest infestations of mollusk, worm, fungus, insects, etc., affecting its health and survival.
ERDB warns that some species of mangroves are more vulnerable to infestation like barnacles
which are considered the most destructive pests of mangroves, inhibiting the growth leads to
mangrove death (BDPH, 2017). Infestation can create large-scale diebacks by feeding and making
homes on barks, trunks and branches of trees and shrubs, weakening the structure of the tree. And
In this perspective, assessing the health of mangrove trees is therefore significant for a
variety of reasons.
In this study the pest infecting the mangrove trees in Sitio Bayug, Brgy. Hinaplanon, Iligan
City is to be determined. The following are the specific objectives that must also be met to
1. to assess the abundance and diversity of the species infesting the mangrove trees in
Sitio Bayug, Brgy. Hinaplanon, Iligan City for the occurrence of infestation and to
2. to determine the prevalence and incidence of the infestation and the specific location
3. to determine the to determine the physico-chemical parameters such as water and soil
pH, air temperature, water temperature, dissolved oxygen, salinity, total suspended
physico-chemical parameters;
tree profile in the said area. These information can contribute as reference for future studies and
strategies in the area and can also be used as baseline source for further development of the area.
The study was limited only on surveying the occurrence of infestation, the extent of
infection, prevalence and incidence. The abundance and diversity of mangroves in the sampling
area will also be determined and the appearance and distribution of pest(s) on the different parts
of the mangrove. And so as to compare infested mangrove tree to a healthy mangrove profile. The
physico-chemical parameters such as D.O., salinity, air and water temperature, water and soil pH,
humidity, total suspended solids, and substrate estimation will be all taken.
Methods
coordinates of 8°15'51.0"N 124°14'56.5"E that is 3.7 km northeast of Poblacion. Bayug was the
earliest pre-Spanish settlement of native sea dwellers in pre-colonial era. Bayug Island was almost
wiped out during a strong tropical storm “Sendong” hit the Philippines in 2011
(opinion.inquirer.net, 2012).
B. Sampling Method
A quadrat and transect line method will be used. Establishing a transect line
perpendicular to the shoreline and fixing the line on both ends. Then, laying out of the 10m
× 10m quadrats at 10m interval from the shore, seawards. All trees within the 10m × 10m
quadrat will be measured of DBH (Diameter at breast height) and will record the number
of individuals through actual count (English et al., 1997.). Samples of tree units (plants,
leaves, inflorescences etc.) will be taken at random from the field (e.g. by following a set
of random number coordinates, or a randomly chosen number of paces across the field) or
across the field and taking a sample every 10 paces) to observe the extent of infestation
and determine the species that infests. Random sampling is preferred by biometricians.
C. Measurement of the Meristic Characteristic of Mangrove
Total height: The Total height will be obtained by estimation using the height of a person.
Leaf cover: A calibrated vinyl measuring tape will be used to determine the leaf cover by
Leaf width: A calibrated plastic ruler will be used to determine the leaf width by getting
five leaves on each tree and each of the leaves width will be measured and the average
Diameter at breast height (DBH): As prescribed in Benfield, 2002 and Defew, 2003 the
diameter of a tree at breast height will be measured using a pre-calibrated DBH tape
measure. To ensure accuracy, the DBH tape should be level and stretched firmly against
the trunk. If the tree has already branched out, both the circumference at breast height as
In studying ecological communities, some indices are used to help assess the overall
health of the ecosystem. These include species richness, relative abundance, dominance
and evenness, and species diversity. Paleontological Statistics Software (PAST) will be
D. Infestation Assessment
Critical information in the assessment of disease is the amount of disease that is present.
population of plants. This can be measured by counting the number of plant that is
infested.
b. Disease Severity: Refers to severity in the quantity of disease affecting plants within a
sample population. This is done by observing the sizes of lesion and its extent (spread)
severity by comparing a diseased plant with diagrams showing both more and less
disease. Standard area diagrams e.g. leaf rust provide 1, 5, 10, 20, 50% leaf area
infected.
Figure 1.1 Disease assessment keys. (A) Common scab of potatoes caused by
Streptomgces scabres. (From James 1971.) (B) Mycosphaerella leaf diseases of
eucalypts. (From Carnegie et al. 1994.) (C) phytophthora blight (P. coLocasise) of
taro (Colocasta esculenta). (From Golliferand Brown, 1974.)
b. Descriptive scales: The descriptive keys have also been standardized for various
diseases. This is widely used and are of many types ranging from disease rating on
0.1 Lesions found with difficulty, and on less than one plant in fifty.
5 Lesions on every plant, and on most leaves except the young ones,
but only about two to ten spots per leaf
10 All except the youngest leaves affected, with ten to fifty spots per
leaf.
25 All except the youngest leaves affected, but with about three-
quarters of the leaf area green, although lowest leaves may be
severely attacked.
50 All leaves affected. Most of the middle leaves showing only half
their area green.
75 All leaves affected. Most of the middle leaves show only one-
quarter of the leaf green, giving a grey appearance to the crop as a
whole.
90 Very little green visible on middle and lower leaves, but youngest
leaves show green.
100 All leaves completely covered with the lesions.
Table 1.2 Key for the assessment of root rot (From Greenhalgh and Lucas, 1984.)
Scale Symptoms
1 Slight lateral root rot (less than 1% of lateral root tips necrotic)
will be done. On each trees, all parasites found will be counted from the roots, the stem and the
leaves. Different species will counted separately and their location on the mangrove parts is noted.
A representative sample per species will be collected and put inside a zip-lock bag and will be
brought to the laboratory, cleaned with fresh water and then identified using FAO’s The Living
Marine Resources of the Western Central Pacific Vol. 1(K. Carpenter and V. Niem 1998).
a. Temperature
Field thermometer will be used to measure the water temperature on site.
b. Salinity
Determination of salinity will be measured with the help of a refractometer.
c. pH
The soil and water pH will be measured using pH meter on site.
e. Dissolved oxygen
The Dissolved oxygen of the seawater in the study area will be determined with the
help of a D.O. meter.
f. Humidity
Relative humidity will be measured using a psychrometer.
F. Statistical Analysis
Pearson Correlation
The correlation between disease infestation and meristic characters will be obtained
References
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Eucatgpttts globulus provenances to Mycosphaerella leaf disease . Canadian Journal of
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