BD Vacutainer  Order of Draw

for Multiple Tube Collections

Designed for Your Safety Reflects change in CLSI recommended
Order of Draw (H3-A5, Vol 23, No 32, 8.10.2)

Closure Color Collection Tube Mix by Inverting

BD Vacutainer  Blood Collection Tubes (glass or plastic)
®

• Blood Cultures - SPS 8 to 10 times

* When using a winged

blood collection set • Citrate Tube* 3 to 4 times
for venipuncture
and a coagulation or • BD Vacutainer  SST
® ™
5 times
(citrate) tube is the Gel Separator Tube
first specimen tube to • Serum Tube 5 times (plastic)
be drawn, a discard (glass or plastic) none (glass)
tube should be drawn
• BD Vacutainer Rapid
®

first. The discard tube 5 to 6 times

Serum Tube (RST)
must be used to fill
the blood collection or • BD Vacutainer  PST
® ™
8 to 10 times
set tubing’s “dead Gel Separator Tube
space” with blood With Heparin
but the discard tube
• Heparin Tube 8 to 10 times
does not need to be
completely filled. This
important step will or • EDTA Tube 8 to 10 times
ensure proper blood-
• BD Vacutainer PPT ™
®

to-additive ratio. The 8 to 10 times

discard tube should
Separator Tube
K2EDTA with Gel
be a nonadditive or
coagulation tube.
• Fluoride (glucose) Tube 8 to 10 times

Note: Always follow

your facility’s protocol
for order of draw BD Technical Services
Handle all biologic samples and blood collection 1.800.631.0174
“sharps” (lancets, needles, luer adapters and
blood collection sets) according to the policies BD Customer Service
and procedures of your facility. Obtain appropriate
medical attention in the event of any exposure
to biologic samples (for example, through a
puncture injury) since they may transmit viral
1.888.237.2762
hepatitis, HIV (AIDS), or other infectious diseases.
Utilize any built-in used needle protector if the
www.bd.com/vacutainer
blood collection device provides one. BD does
not recommend reshielding used needles, but
the policies and procedures of your facility may
differ and must always be followed. Discard any
1 Becton Drive
Franklin Lakes, NJ 07417
blood collection “sharps” in biohazard containers
approved for their disposal.
= 1 inversion
www.bd.com/vacutainer BD, BD Logo and all other trademarks are property of Becton, Dickinson and Company. © 2010 BD
Franklin Lakes, NJ, 07417 1/10 VS5729-6
 BD Microtainer® Tubes with BD Microgard™ Closure
Tube Guide and Order of Draw
Mix by
Catalog #/Closure Color Additive Inverting Laboratory Use

For whole blood

hematology determinations.
K2EDTA 10x Tube inversions
prevent clotting.
365974 Lavender

For plasma determinations

Lithium Heparin 10x in chemistry. Tube inversions
prevent clotting.
365965 Green

Lithium Heparin For plasma determinations

365985 Mint Green
and Gel for 10x in chemistry. Tube inversions
plasma separation prevent clotting.

365987 Mint Green

365976
Tube Extender
For glucose
determinations. Tube
NaFl/Na2EDTA 10x inversions ensure
proper mixing of
additive and blood.
365992 Grey

Clot Activator
365967 Gold and Gel for 5x For serum determinations
serum separation in chemistry.

365978 Gold

No additive 0x For serum determinations

in chemistry.
365963 Red

BD Global Technical Services: CAUTION: BD Diagnostics

1.800.631.0174
BD Customer Service:
1.888.237.2762
www.bd.com/vacutainer
Handle all biologic samples and blood collection “sharps” (lancets, needles, luer adapters, and blood collection sets) Preanalytical Systems
in accordance with the policies and procedures of your facility. Obtain appropriate medical attention in the event of 1 Becton Drive
any exposure to biologic samples (e.g., through a puncture injury) since samples may transmit viral hepatitis, HIV (AIDS),
Franklin Lakes, NJ 07417
or other infectious diseases. Utilize any safety-engineered feature if the blood collection device provides one. Discard
all blood collection “sharps” in biohazard containers approved for their disposal.

BD, BD Logo and all other trademarks are property of Becton, Dickinson and Company. ©2004 BD. Made in USA 11/04 VS5836-2