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3
The light-independent
reaction
Aims
This worksheet requires you to study and compare the Calvin cycle with alternative ways of
fixing carbon dioxide. You will need to discuss the advantages of each new pathway and
understand the conditions that make them beneficial. You will need a good knowledge of the
Calvin cycle to complete this worksheet.
Questions
1 Outline the events that take place in a C3 plant during the fixation of carbon dioxide
(the Calvin cycle).
2 Although C4 plants are more efficient at high light intensities and high temperatures, low light
intensities and low temperatures favour C 3 plants. Suggest why C4 plants are more efficient at
higher light intensities and higher temperatures.
3 The chain of reactions initiated when RuBP accepts oxygen is called photorespiration. Suggest why.
4 The molecule that combines with carbon dioxide in C4 plants is PEP but in C3 plants the molecule is
RuBP. PEP has a much higher affinity for carbon dioxide than RuBP and will not accept oxygen.
Explain how this helps C4 plants.
5 Gas exchange in CAM plants takes place mainly at night as this is when the CAM pathway fixes
carbon dioxide. Explain how this helps these plants avoid excessive water loss.
6 The main difference between C4 and CAM pathways is the way by which carbon dioxide supply to
the Calvin cycle is maintained. Explain how the carbon dioxide supply is maintained and what the
benefit to the plant is in each case.
7 In what ways does the C4 pathway differ from the CAM pathway?
8 Genetic modification of plants may make it possible to transfer the genes for the C 4 or CAM
pathways to C3 plants. As a result the genetically modified plants could be more efficient and better
adapted to the conditions in many developing countries. What would a scientist consider before
deciding to genetically modify a C3 plant with genes from one of the C4 or CAM pathways?