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Siti Nor Syairah Anis, Nurhezreen Md Iqbal, Sudesh Kumar & Amirul Al-
Ashraf
To cite this article: Siti Nor Syairah Anis, Nurhezreen Md Iqbal, Sudesh Kumar & Amirul Al-Ashraf
(2013) Increased recovery and improved purity of PHA from recombinant Cupriavidus necator,
Bioengineered, 4:2, 115-118, DOI: 10.4161/bioe.22350
Download by: [Universiti Sains Malaysia] Date: 23 December 2017, At: 11:18
Article Addendum Article Addendum
Bioengineered 4:2, 115–118; March/April 2013; © 2013 Landes Bioscience
A
Downloaded by [Universiti Sains Malaysia] at 11:18 23 December 2017
simple procedure for recovering depends on several factors. The main fac-
biodegradable polymer from bacte- tor is the chemicals or solvents used for
rial cells has been developed using eco- recovery process which should be a green
nomical and environmentally friendly chemical or green solvent. It will be a more
solvent or chemicals. Recombinant bac- economical process if the green chemical
terium, Cupriavidus necator harboring or green solvent is an inexpensive material.
pBBR1MCS-C2 plasmid polyhydroxy- Besides that, a simple process during oper-
alkanoate (PHA) synthase gene was ation of the PHA extraction which reduces
used for the production of copolymer the energy, time and cost should also be
P(3HB-co-3HHx) from crude palm ker- taken into consideration especially in the
nel oil (CPKO). NaOH was chosen in large scale recovery process.
this study as it could give high purity PHA is an intracellular product, thus
and recovery yield. Increase of NaOH the methods adopted for its recovery focus
concentration had resulted in an increase either on its solubilization or on the solu-
of the PHA purity, but the recovery yield bilization of non-polymer cellular materi-
had decreased. The greater improve- als (NPCM). The NPCM which consist
ment of PHA purity and recovery were of nucleic acids, lipids, phospholipids,
achieved by incubating the freeze-dried peptidoglycan and proteinaceous materi-
cells (10–30 g/L) in NaOH (0.1 M) for als will be separated out from the polymer
1–3 h at 30°C and polishing using 20% during recovery process.2,3
(v/v) of ethanol. The treatment caused There are many different strategies for
negligible degradation of the molecu- the extraction and recovery of the polymer
Keywords: PHA, recovery, Cupriavidus lar weight of PHA recovered from the or PHA from bacterial cells. Numerous
necator, alkaline digestion, P(3HB-co- bacterial cells. The present review also recovery processes have been proposed
3HHx) highlights other extraction methods to to recover PHA from C. necator. These
provide greater insights into economical involve solvent extraction, enzymatic diges-
Submitted: 07/04/12 and sustainable recovery of PHA from tion method, sodium hypochlorite diges-
Revised: 09/23/12 bacterial cells. tion and the use of mechanical process
method.4-6 However, these methods are
Accepted: 09/24/12
The biodegradable polymer is not highly generally complex, expensive, highly toxic,
http://dx.doi.org/10.4161/bioe.22350 competitive to petrochemical-based plas- cause severe degradation of molecular
*Correspondence to: Amirul Al-Ashraf; tics due to its high production and recovery weight of polymer and require large quan-
Email: amirul@usm.my cost. Particularly, the cost of PHA recovery tities of non-environmental friendly sol-
from bacteria cells is more than 50% of the vents.4 Due to this, a simple, efficient and
Addendum to: Anis SN, Nurhezreen MI, Sudesh total PHA production cost as revealed by more economical recovery method using
K, Amirul AA. Enhanced recovery and purifica-
economic evaluation.1 For large scale pro- green chemicals or solvents is developed in
tion of P(3HB-co-3HHx) from recombinant
Cupriavidus necator using alkaline digestion duction, PHA extraction cost should be as this research (Fig. 1). The green chemicals
method. Appl Biochem Biotechnol 2012; 167:524- low as possible with high PHA recovery or solvents are environmentally friendly
35; PMID:22569781; http://dx.doi.org/10.1007/ yield at the end of this product. The effi- and their usage can result in reduced waste,
s12010-012-9677-9 cient recovery process for PHA production safer outputs and eliminated pollution.
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