5th ANNUAL North Dakota Tribal College Research Symposium

Disseminating the Future of Tribal College Research

Friday, April 7, 2017

Sponsored by: ND IDeA Network for Biomedical Research Excellence

ABSTRACT SUBMISSION GUIDELINES
Deadline: March 31, 2017

ELECTRONIC SUBMISSION BY E-MAIL

To: lacey.mckay@littlehoop.edu
Subject: NDTCRS Abstract Submission
Attach your abstract

Instructions for Preparing Abstracts

1. All abstracts must be sent via email as attached documents formatted in Microsoft WORD.
Please follow the directions carefully and use the format indicated.
2. Use the sample abstract format as a guide for size as you prepare your abstract.
3. The abstract content should be structured as follows:
► A specific and detailed title [bolded]
► Authors [First name, Middle Initial, Last name] Note: Do not include degrees after the
Authors’ names. Place an asterisk before the name of the presenting author.
► Single space after the Title and Authors.
► Single-space the text of the abstract with one continuous paragraph using Times New Roman
12 CPI.
► Text should be no more than 250 words (not including title, authors, and contact info)
► Organize the text in the following manner:
o A brief Purpose statement or Background of the study
o A statement of the Methods used (including number of subjects and other
pertinent data)
o A summary of the Results presented in sufficient detail to support the conclusion
o A statement of the Conclusion which should include potential impact or public
health use for native populations (it is not acceptable to state “the results will be
discussed”)
► Single line spacing after the text of the abstract.

4. Complete the biographical sketch on the next page and email it to the address below.
6. Abstracts must be received by close of business on March 31, 2017.
7. All abstracts should be emailed to Lacey McKay at: lacey.mckay@littlehoop.edu.

Please put NDTCRS Abstract Submission in the subject heading. For questions about abstract
submission, please contact Lacey by email or by telephone at 701-766-1323 (email preferred).

5th Annual North Dakota Tribal College

Research Symposium

Biographical Data Form

Primary Author: (Name, Title, STUDENT and or Place of Employment)

As you would like it to appear in the program listing

Email Address:

Mailing Address:

City/State/Zip:

Phone Numbers: Work: Fax:

Secondary Authors: (Name, Title, STUDENT and or Place of Employment)

Submitted for: Poster Presentation

 4th Annual ND Tribal College Research Symposium
Abstract Template

An Investigation into the Differential Expressions of SCN1A and PFKFB3 Based on Absence
or Presence of the N- or C-Terminal Domains of MT-3
Derrick Fournier*, Brent J Voels, Scott H Garrett, Don A Sens, Seema Somji
Department of Pathology, School of Medicine and Health Sciences, 501 N. Columbia Road Stop
9037,University of North Dakota, Grand Forks, ND 58202-9037.
Studies have shown that the MT-3 protein contains 7 additional amino acids that are not present in
any other member of the MT gene family, a 6 amino acid C-terminal sequence and a Thr in the N-
terminal region. The goal of this study was to characterize the function of the N- and C-terminal
domains of MT-3 in the breast cancer cell line, MCF-7. For this purpose six different constructs of
MTs were prepared which were as follows: wild type (WT) MT-3, MT-3 N-terminal mutation,
MT-3 C-terminal deletion, WT MT-1E, and MT-1E mutated to contain the N-terminal of MT-3 or
the C-terminal or both the N- and the C-terminal of MT-3. Each of these constructs was stably
transfected into MCF-7 cells and subsequent microarray analysis was performed. Microarray
analysis demonstrated that Sodium Channel, Voltage Gated, Type 1 (SCN1A) and 6-
phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3) expression were up regulated 2.35
and 2.1 fold respectively. It has been shown that voltage gated sodium channels (VGSCs) are up
regulated in cancer, and favor an invasive/metastatic phenotype. Array validation demonstrated
that SCN1A expression was not significantly altered. PFKFB3 is highly expressed and activated in
cancer cells specifically colon adenocarcinoma. Real time PCR analysis indicated that PFKFB3 is
significantly up regulated only when the N-terminal of MT-3 is present, but down regulated when
only C-terminal is present. Differential regulation of the N- or C-terminal domains of MT-3 may
affect tumor progression due to the alteration of PFKFB3 expression.