Professional Documents
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(Bioecology of anthracnose pathogen (Colletotrichum spp.) Origin of Plant Papaya (Carica papaya
L.) 2
ABSTRACT
Papaya has many beneficial effects on human utility that is produce food substance.
Colletotrichum spp. is one of limiting factor of papaya production that cause anthracnose. This
pathogens can infect different organs of the papaya plant, so it is Necessary to detect the presence
of the fungus in the field and to identify morphological characteristics as well as the ability to
infect in various organs of the plant. The aims of studying bioechology of anthracnose
(Colletotrichum spp.) Crops origin papaya (Carica papaya L.) in West Java is will be conducted
using methods of isolation, identification, pathogenicity test, cross pathogenicity in various
different organs of the plant papaya and the ability of transmitted in seed. This experiment uses a
completely randomized design (CRD) then conducted a further test of Duncan's Multiple Range
Test (DMRT). The expected benefits from this research are to provide scientific information in
particular Offender agricultural cultivation horticulture and ecology of the species origin papaya
fruit anthracnose fungi will be used in controlling anthracnose.
PRELIMINARY
Background
Papaya (Carica papaya L.) is one of the fruits are highly favored by the people, including
Indonesia, it is seen from the increasing per capita consumption of papayas from year to year.
Consumption of papaya in 2002 was nearly 2:24 kg per capita with a population in 2002 amounted
to 212 million. If the estimated 2025 total population of 315 million inhabitants, the consumption
of papaya Indonesia reached 705 600 tonnes. Indonesia ranks fifth largest papaya producer world
with a production of 621 524 tonnes (FAO 2007). The amount of production in Indonesia because
papaya papaya has some privileges compared with other types of fruit, because it is easily
cultivated, fast to produce and can be grown throughout the year. One of the limiting factors in the
increased production of both quality and quantity of papaya fruit is anthracnose pathogen that
attacks when post-harvest. Pathogens that infect the fleshy fruit is caused by the fungus
Colletotrichum spp. during the pre-harvest and remain latent infections develop into symptoms of
the disease in the post-harvest.
According to Bailey and Jeger (1992), anthracnose caused by Colletotrichum species are important
pathogens that affect the economies of tropical and sub-tropical areas that can infect the host in
such a wide range of horticultural crops and food. Within 5 years in Mexico, the spread of
anthracnose in the field or in storage can reduce yields by 50% although it has been repeated
fungicide applications. Kader (2000) also states that anthracnose caused by Colletotrichum
gloeosporioides is a major cause of yield loss on papaya fruit after harvest in California. The
disease is caused by a species of C. capsici and C. gloeosporioides in Mexico, USA, Trinidad and
Tobago (Tapia-Tussell et al., 2008; Tarnowski and Ploetz 2010; Rampersad, 2011), while in
Malaysia (Sepiah et al. 1991) report that pathogens are important causes anthracnose on papaya is
C. capsici. In Indonesia, the papaya fruit anthracnose pathogen is C. gloeosporioides (Penz) Sacc
that the perfect stage known as Glomerella cingulata (solution would et al. 1991). The symptoms
appear when the fruit is in the transport, marketing or storage while in the field is still in a state of
latent pathogens. Symptoms caused by Colletotrichum spp. initially formed small brownish-black
spots on the fruit, yellowish edges and widened in humid conditions. By the time the symptoms
last more symptomatic section will form the body of the fruit in concentric circles forming pink
spore masses (Semangun 2007), the symptoms will appear at 5 days after infection. Conidia were
spherical to elongated spherical, not septate and sometimes contain 1-2 dots of different sizes.
Fungus conidia size varies by an average of 12-16 x 4-6 μm (Singh 1991).
Relatively fast development of anthracnose disease was suspected by the air humidity is high
enough 78.5% -82.95%, rainfall of 2937.1 mm (Romlawati 1999) and the optimum temperature
for growth of C. gloeosporioides is 28 ºC (AVRDC 1988). The distribution is relatively fast
because on the outside of the spores of this fungus has an adhesive so that it can easily be attached
by sprinkling water on the target (Suryaningsih et al. 1996) so that the spores will germinate
quickly when you've found the right host.
Generally the species Colletotrichum spp. can be identified from a range of growth and
morphological characteristics such as morphology of conidia, presence or absence of seta,
fungicide sensitivity, colony color and growth rate (Adaskaveg and Hartin 1997). For that to
identify the causes anthracnose fungus to determine the similarity between plant species and the
effect of temperature on the growth of pathogens. It can be used as supporting information to
determine the appropriate method in controlling anthracnose.
Formulation of the problem
Anthracnose fungus infects papaya fruit when post-harvest but can also attack during the process
of planting in the field. Further research to explore the causes anthracnose and identification of
species from various parts of the papaya plant.
Research purposes
This study aims to identify the anthracnose pathogen in various parts of the plant papaya (Carica
papaya L.), and compare the species Colletotrichum spp. in a variety of areas that include
pathogenicity test, the effect of temperature on the growth, and test seed-borne pathogens.
hypothesis
The species of fungi Colletotrichum spp. which infects the fruit, leaves, petioles and stems are the
same. This is associated with the expression of symptoms and morphological similarity fungi on
each part of the plant in various areas as well as the differences in growth rate of pathogens on
variations in temperature, and the ability to carry over seed.
Benefits of research
This research is expected to provide scientific information in particular offender agricultural
cultivation of horticultural ecology on the type and origin of papaya fruit anthracnose fungi for the
preparation of appropriate strategies in the control of anthracnose.
Method
Isolation of Colletotrichum spp.
The fungus was isolated from the stem, branches, fruit and papaya on each section showing typical
symptoms of anthracnose. Isolation of fungi carried out in the laboratory using the method of
planting media network on potato dextrose agar (PDA) and incubation at 28 ° C. Pure culture
conidia harvested after 7 days. Harvesting is done by adding sterile water to the cup so submerged
(± 18 mL). Conidia were harvested using a spatula to manufacture a single spore cultures. Single
conidia obtained culture propagated back on PDA and incubated at room dark by irradiating 12
hours and 12 hours of light under near ultraviolet rays for seven days.
Data analysis
The experiments were performed using a completely randomized design (CRD) and descriptive
data analysis, the results of experiments analyzed using ANOVA with SAS 9.1. The treatment that
significantly tested further by Duncan's multiple range test at 5% significance level.
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