Number of amino acids: 446

Molecular weight: 49912.23

Theoretical pI: 4.81

CSV format
Amino acid composition:
Ala (A) 40 9.0%

Arg (R) 17 3.8%

Asn (N) 31 7.0%

Asp (D) 33 7.4%

Cys (C) 2 0.4%

Gln (Q) 22 4.9%

Glu (E) 21 4.7%

Gly (G) 49 11.0%

His (H) 6 1.3%

Ile (I) 20 4.5%

Leu (L) 22 4.9%

Lys (K) 20 4.5%

Met (M) 16 3.6%

Phe (F) 20 4.5%

Pro (P) 9 2.0%

Ser (S) 28 6.3%

Thr (T) 25 5.6%

Trp (W) 19 4.3%

Tyr (Y) 22 4.9%

Val (V) 24 5.4%

Pyl (O) 0 0.0%

Sec (U) 0 0.0%

(B) 0 0.0%

(Z) 0 0.0%

(X) 0 0.0%

Total number of negatively charged residues (Asp + Glu): 54

Total number of positively charged residues (Arg + Lys): 37

Atomic composition:

Carbon C 2221

Hydrogen H 3287

Nitrogen N 601

Oxygen O 683

Sulfur S 18

Formula: C2221H3287N601O683S18

Total number of atoms: 6810

Extinction coefficients:

Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.

Ext. coefficient 137405

Abs 0.1% (=1 g/l) 2.753, assuming all pairs of Cys residues form cystines

Ext. coefficient 137280

Abs 0.1% (=1 g/l) 2.750, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).

>20 hours (yeast, in vivo).

>10 hours (Escherichia coli, in vivo).

Instability index:

The instability index (II) is computed to be 29.97

This classifies the protein as stable.

Aliphatic index: 61.30

Grand average of hydropathicity (GRAVY): -0.516

Longueur de la séquence : 446 amino acides

Masse moléculaire : 49912,03
Coefficient d'extinction molaire à 280 nm (1cm) : 136270
- en prenant en compte toutes les cystéines : 136390

Absorption 0,1% (1g/l) à 280 nm (1cm) : 2,730

- en prenant en compte toutes les cystéines : 2,733

Point isoélectrique théorique :

- classique : 4,60
- en prenant en compte toutes les cystéines : 4,60

Index aliphatique : 61,30

MMITLRKLPL AVAVAAGVMS AQAMAVDFHG YARSGIGWTG SGGEQQCFQT
TGAQSKYRLG NECETYAELK LGQEVWKEGD KSFYFDTNVA YSVAQQNDWE
ATDPAFREAN VQGKNLIEWL PGSTIWAGKR FYQRHDVHMI DFYYWDISGP
GAGLENIDVG FGKLSLAATR SSEAGGSSSF ASNNIYDYTN ETANDVFDVR
LAQMEINPGG TLELGVDYGR ANLRDNYRLV DGASKDGWLF TAEHTQSVLK
GFNKFVVQYA TDSMTSQGKG LSQGSGVAFD NEKFAYNINN NGHMLRILDH
GAISMGDNWD MMYVGMYQDI NWDNDNGTKW WTVGIRPMYK WTPIMSTVME
IGYDNVESQR TGDKNNQYKI TLAQQWQAGD SIWSRPAIRV FATYAKWDEK
WGYDYTGNAD NNANFGKAVP ADFNGGSFGR GDSDEWTFGA QMEIWW

Composition en amino acides

Ala (A) 40 8,97 %
Arg (R) 17 3,81 %
Asn (N) 31 6,95 %
Asp (D) 33 7,40 %
Cys (C) 2 0,45 %
Gln (Q) 22 4,93 %
Glu (E) 21 4,71 %
Gly (G) 49 10,99 %
His (H) 6 1,35 %
Ile (I) 20 4,48 %
Leu (L) 22 4,93 %
Lys (K) 20 4,48 %
Met (M) 16 3,59 %
Phe (F) 20 4,48 %
Pro (P) 9 2,02 %
Ser (S) 28 6,28 %
Thr (T) 25 5,61 %
Trp (W) 19 4,26 %
Tyr (Y) 22 4,93 %
Val (V) 24 5,38 %

Asx (B) 0 0,00 %

Glx (Z) 0 0,00 %

Courbe de tirage
Valeurs utilisées des pK :
alpha-NH3 : 9,69 alpha-COOH : 2,34
Arg : 12,40 Lys : 10,50 His : 6,00
Asp : 3,86 Glu : 4,25 Cys : 8,33 Tyr : 10,00