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First two digits represents type of reaction it catalyzed, last two digits
represents substrate which enzyme acts.
Properties of enzymes
1. Enzyme is specialized protein
It exhibit all properties of proteins
2. Colloidal nature : Due to large size of molecules
3. Catalytic property
Enzymes are very efficient catalyst. Only small amount of
enzymes is enough to convert large quantity of substrate in
product. It Speed up reaction by 106 - 1012 times greater than
those of the corresponding un catalyzed reactions
4. Specific
Enzymes are more specific toward their substrates and for the type of
reactions that catalyze.
Enzymes shows 3 types of specificity
a. Specific specificity
Some enzymes act on only one substrate
e.g. Urease acts only on urea.
b. Group specificity
Catalyze the reaction of structurally related groups only
Amylase hydrolyses the group of substances like starch, dextrin and
glycogen, which have the same type of glycosidic linkages (α1,4).
c. Optical Specificity
The enzymes acts on only one of the two optical isomers of a compound
For example L-amino acid oxidase acts only on L-amino acid but not on
its D-form of amino acid.
6. pH sensitivity
Enzyme is dependent upon the pH of where the reaction is taking
place, e.g. pepsin in the stomach has an optimum pH of about pH2,
Whereas salivary amylase has an optimum pH of about 7. 4.
7. Reversibility of reaction
Enzymes are capable of bringing reversion in a chemical reaction
Function of enzymes
1) To accelerate or retard or bring about reaction
2) Regulate reaction
3) To make possible the metabolic reactions
4) To facilitate reaction
5) To break down larger molecule to small molecule
6) To carry out flow of reaction smoothly
a. Michaelis-Menton Hypothesis
The theory postulates that the enzymes has number of active sites where
the substrate is bounded to form weak enzyme substrate complex (S).
This enyzme-substrate complex, on hydrolysis, decomposes to yield the
reaction product (P) and the free enzyme (E). These reactions may be
symbolically represented as follows:
[S] + [E] ----------> [ES] ---------> P+[E]
POLYLACTIC ACID
Chemical factors
• Chemical structure & composition
• Presence of ionic group and configuration structure
• Molecular weight and presence of low molecular weight compounds
Physical factors
• Processing condition
• Sterilization process
Waste Management
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