Serotonin Receptor (5HT1A) Polyclonal Antibody

Product Specifications Background:

Catalog Number: 905-741 Serotonin (5-hydroxytryptamine, 5-HT) is a
monoamine found in the central nervous system,
Host: Rabbit gastrointestinal tract, and blood with broad
physiological functions as a neurotransmitter, in gastric
Species Reactivity: Human, Mouse, Rat motility, hemostasis, and cardiovascular integrity1.
Other species not tested
Seven subclasses (5-HT1-7) of serotonin receptors have
Applications: WB: Yes been identified, a majority of which are members of
IHC: Yes the seven transmembrane G-protein coupled receptor
Membrane ELISA*: Yes (GPCR) family, and are the target of several anti-
Cell ELISA*: Yes depressant drugs2. The 5-HT1 subclass consists of five
Other applications not tested.
highly homologous receptors that couple via Gi/o 
The optimal dilution for a specific application subunits to inhibit adenylyl cyclase activity3. The
must be determined by the investigator. 5-HT1A receptor gene is 99% homologous between
*Under certain conditions, this antibody has human and mouse, and is expressed from an intronless
been shown to display activation-state gene with a predicted length of 421 amino acids.
specificity5. The dilution required to achieve
activation-state specificity will vary, and 5-HT1A receptor activation is generally inhibitory to
should be optimized by the researcher. cAMP production, but may also stimulate type II
adenylyl cyclase activity in a cell-specific manner via
Predicted M.W.: ~46 kDa. Higher m.w. species release of Gβγ subunits when coupled to Gi24. 5-HT1A
(~50 kDa) and intermediates also expression in mammals is strongest in CNS limbic
observed which may reflect post- pathways that control emotion, including tissue of the
translational modification or hippocampus, dorsal raphé, and septum1.
dimerization of the receptor6,7.
Concentration: See product label References:
Purification: Peptide Affinity 1. Pineyro, G. and Blier, P. (1999) Pharmacol Rev. 51, 533-591.
2. Hoyer, D., et al. (1994) Pharmacol Rev. 46, 157-203.
Format: PBS, 50% glycerol, 0.01% 3. Lin, S.L., et al. (2002) Br J Pharmacol 136, 1072-1078.
4. Albert, P.R., et al. (1999) J Biol Chem. 274, 35469-35474.
sodium azide 5. Gupta, A., et al. (2007) J Biol Chem. 282, 5116-5124.
6. Ou, X.M., et al. (2001) J Biol Chem. 276, 14299-14307.
Storage: Store at -20˚C 7. Lukasiewicz, S., et al. (2007) Pharmacol Rep. 59, 379-392.
Shipping conditions may differ from the
recommended storage temperature

Immunogen: Synthetic peptide derived from

sequence near the amino-
terminus of mouse Serotonin
Receptor (5-HT1A)
Related Products:
Goat anti-Rabbit IgG Polyclonal
SAB-300
Antibody, HRP Conjugate
900-066 cyclic AMP (direct) EIA Kit Visit the Scientific Resources
section of our website for
Serotonin Monoclonal Antibody
905-548 ELISA, IHC, and WB protocols.
(SPM146)

Generally reagents are good for one year from the date of receipt, except for conjugates which are good for six months and
reagents with an expiration date indicated on the label or other supporting document.

Assay Designs makes every effort to provide a consistent source of high quality polyclonal antibodies. However, due to variations inherent in this
technology, investigators are urged to purchase sufficient quantities of a specific lot number if an identical antibody is required throughout a study.

(OVER)

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC USE

5777 Hines Drive ● Ann Arbor, MI ● 48108 | Tel: 800-833-8651 or 800-668-6113 | Fax: 734-668-2793
www.assaydesigns.com | orders@assaydesigns.com | technical@assaydesigns.com
Last Revised: 7-13-2009
 Western Blot Analysis: MW marker
(1) and 20 g rat brain extract (2)
probed with Serotonin Receptor (5HT1A)
Polyclonal Antibody at 2 µg/mL.

IHC: Fluorescent confocal

photomicrograph of rat brain cortex
fixed in 100% methanol was stained
with propidium iodide (red) and
Serotonin Receptor (5HT1A) Polyclonal
Antibody (green) at 1:1000.

Cellular ELISA: HEK293 cells expressing

serotonin receptor 5HT1A were treated with
indicated concentrations of agonist (DPAT)
and probed with Serotonin Receptor
(5HT1A) Polyclonal Antibody (1:1000 of a
stock solution of 1 μg/μL) by ELISA. Data
from vehicle treated cells were taken as
100%. Results are the mean ± SEM
(n=3).

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC USE

5777 Hines Drive ● Ann Arbor, MI ● 48108 | Tel: 800-833-8651 or 800-668-6113 | Fax: 734-668-2793
www.assaydesigns.com | orders@assaydesigns.com | technical@assaydesigns.com
Last Revised: 7-13-2009