Natural Products 2016 Scientifictracks Abstracts

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com
575th Conference
International Conference and Exhibition on
Marine Drugs and

Natural Products
July 25-27, 2016 Melbourne, Australia
Scientific Tracks & Abstracts
(Day 1)
Natural Products 2016

Tracks 1, 2, 6, 7 & 12
Day 1 July 25, 2016
Track 1: Natural Products
Track 2: New Sources and Approaches to Natural Products
Track 6: Medicinal Natural Products
Track 7: Natural Products as Anti-Cancer Drugs
Track 12: Bioactive Natural Products
Session Chair Session Chair
Jae-Ha Ryu Liang Xu
Sookmyung Womens University, Republic of Korea University of Kansas Cancer Center, USA
Session Introduction
Title: Natural products targeting RNA-binding protein Musashi
Liang Xu, University of Kansas Cancer Center, USA
Title: Production of biomass and secondary metabolites from plant cell and organ culture using bioreactor
system in medicinal plants
So-Young Park, Chungbuk National University, Republic of Korea
Title: Plant derived diarylheptanoids as Wnt/β-catenin pathway inhibitors
Jae-Ha Ryu, Sookmyung Womens University, Korea
Title: Synthesis of analogs of natural tubulin polymerization inhibitors using parsley and dill seed extracts
Victor V Semenov, Russian Academy of Sciences, Russia
Title: “Scytonemin” pigment from Lyngbya notarisii (Cyanobacteria) may protect human skin from UV
radiations.
Abdul Aziz, University of Dhaka, Bangladesh
Title: Anti-diabetic and anti-obesity potential of selected medicinal plants used in the traditional medicine
of Jordan
Fatma U Afifi-Yazar, The University of Jordan, Jordan
Title: Efficacy of detogen-B, a medicinal plant formulation in cancer diseases
Ekenna Elikee, Ekenna Natures Ltd., Nigeria
Title: Food plants and plant molecules affect ovarian functions
Alexander V Sirotkin, Research Institute of Animal Production and Constantine the Philosopher University, Slovakia
Title: Isolation and characterization of a hyperbranched proteoglycan from Ganoderma lucidum for anti
diabetes
Ping Zhou, Fudan University, China

Page 30
conferenceseries.com
Liang Xu, J Pharmacogn Nat Prod 2016, 2:3(Suppl)
http://dx.doi.org/10.4172/2472-0992.C1.002
Marine Drugs and Natural Products

Natural products targeting RNA-binding protein Musashi
Liang Xu
University of Kansas Cancer Center, USA
M usashi-1 (MSI1) is an RNA-binding protein that acts as a translation activator or repressor of target mRNAs. The best-
characterized MSI1 target is NUMB mRNA, whose encoded protein negatively regulates Notch signaling. Additional MSI1
targets include the mRNAs for the tumor suppressor protein APC that regulates Wnt signaling and the cyclin-dependent kinase
inhibitor P21WAF-1. We hypothesized that increased expression of NUMB, P21 and APC, through inhibition of MSI1 RNA-binding
activity might be an effective way to simultaneously down-regulate Wnt and Notch signaling, thus blocking the growth of a broad
range of cancer cells. We used a fluorescence polarization assay to screen for small molecules that disrupt the binding of MSI1 to
its consensus RNA binding site. One of the top hits was (-)-gossypol (Ki=476±273 nM), a natural product from cottonseed, known
to have potent anti-tumor activity and which has recently completed Phase IIb clinical trials for prostate cancer. Surface Plasmon
resonance and nuclear magnetic resonance studies demonstrate a direct interaction of (-)-gossypol with the RNA binding pocket of
MSI1. We further showed that (-)-gossypol reduces Notch/Wnt signaling in several colon cancer cell lines having high levels of MSI1
with reduced SURVIVIN expression and increased apoptosis/autophagy. Finally, we showed that orally administered (-)-gossypol
inhibits colon cancer growth in a mouse xenograft model. Our study identified (-)-gossypol as a potential small molecule inhibitor of
MSI1-RNA interaction and suggests that inhibition of MSI1’s RNA binding activity may be an effective anti-cancer strategy.
Biography
Liang Xu has completed his Post-doctorate training at Stanford University and started his own Lab at University of Michigan working on cancer drug discovery. He is a Co-
Inventor of the first natural product Bcl-2 inhibitor that entered into clinical trials. He has more than 25 patents with four INDs in advanced clinical trials. He is a Professor of
Cancer Biology at University of Kansas and has been funded by NIH, DOD and Komen Foundation. He is currently working on cancer drug discovery targeting the so far
undruggable oncoproteins such as RNA-binding proteins.
xul@ku.edu
Notes:
J Pharmacogn Nat Prod

ISSN: 2472-0992 JPNP an open access journal
Natural Products 2016 Volume 2, Issue 3(Suppl)
July 25-27, 2016

Page 31
So-Young Park, J Pharmacogn Nat Prod 2016, 2:3(Suppl)
http://dx.doi.org/10.4172/2472-0992.C1.002

Production of biomass and secondary metabolites from plant cell and organ culture using bioreactor
system in medicinal plants
So-Young Park
Chungbuk National University, Republic of Korea
P lants have been an important source of pharmacologically active substances for thousands of years. It is estimated that
approximately one quarter of all prescribed drugs contain plant extracts or active ingredients obtained from modeled on plant
substances. Recently, increased emphasis is on the research of bioactive products from plants with potential pharmacological activity.
Plant cell and tissue culture technology has been considered as a powerful tool for the biomass and bioactive compound production
from those medicinal plants. In the past decade, tremendous progress has been made in this area and its importance has rapidly
increased because of increased need for medicinal plant substances as sources of medicine and health food ingredients. Bioreactor
culture system was applied for biomass and secondary metabolite production in medicinal plants. This system has been also refined
to enhance the efficiency in terms of productivity and for cost reduction. For an efficient large-scale bioreactor culture, a perpetual
explant source that is stable and fast growing is important. Majority of studies have been conducted on the cell and root cultures for
biomass and secondary metabolite production for commercial purposes. Herein I would like to present an updated and comprehensive
overview of in vitro biomass production system in various medicinal plants. Future perspectives of biomass and bioactive compound
production have been also discussed.
Biography
So-Young Park has completed her PhD from Chungbuk National University during 1999-2002 in Korea and Postdoctoral studies from University of British Columbia,
Canada. She is the Associate Professor of Department of Horticultural Science in Chungbuk National University. She has published more than 80 papers in reputed journals
and has been serving as an Editorial Board Member of Journals such as Journal of Plant Biotechnology.
soypark1021@gmail.com
Notes:

July 25-27, 2016

Page 32
Jae-Ha Ryu, J Pharmacogn Nat Prod 2016, 2:3(Suppl)
http://dx.doi.org/10.4172/2472-0992.C1.002

Plant derived diarylheptanoids as Wnt/β-catenin pathway inhibitors
Jae-Ha Ryu
Sookmyung Womens University, Republic of Korea
T he Wnt/β-catenin signaling pathway plays a primary role in the differentiation, proliferation, and function of many cells but
disruption of the pathway is involved in cancer development including colon cancer. Colorectal cancer is the third most common
cancer in males and the second most common cancer in females, accounting for approximately 10% of all cancer-related deaths.
Inhibiting the Wnt/β-catenin pathway can be a good strategy for chemoprevention and treatment of colorectal cancer. While screening
for Wnt/β-catenin pathway inhibitors from medicinal plants, we found that (E)-7-(4-hydroxy-3-methoxyphenyl)-1-phenylhept-4-
en-3-one (compound 1), among six diarylheptanoids from lesser galangal (Alpinia officinarum), most potently suppressed Wnt3a-
induced β-catenin/Tcell factor activity. Moreover, compound 1 suppressed proliferation of colon cancer cells by inhibiting β-catenin
translocation to the nucleus by disrupting the β-catenin/galectin-3 complex. Furthermore, a structure–activity realtionship study
implicated that the enone group in the linker is critical and the hydroxy substituent on the aromatic ring is generally preferred for
activity. Our findings suggest that diarylheptanoids from lesser galangal exerts anticolon cancer activity by down regulating the
Wnt/β-catenin pathway. Bioactive diarylheptanoids and the basic understanding of their structure–activity relationship could be
utilized to develop potential candidates for β-catenin-targeted cancer treatment.
Biography
Jae-Ha Ryu has completed his PhD at the College of Pharmacy, Seoul National University, Korea in 1989 and Post-doctoral studies from National Institute of Health,
Maryland, USA. He got academic postion in 1992 at College of Pharmacy, Sookmyung Women’s University, Seoul Korea. He is the Director of Research Center for Cell Fate
Control (Medical Research Center). His main research interest is to suggest leading compounds from medicinal plants for drug development, especially for the treatment
of cancer and various metabolic diseases. He has published 150 papers in reputed journals.
ryuha@sookmyung.ac.kr
Notes:

July 25-27, 2016

Page 33
Victor V Semenov et al., J Pharmacogn Nat Prod 2016, 2:3(Suppl)
http://dx.doi.org/10.4172/2472-0992.C1.002

Synthesis of analogs of natural tubulin polymerization inhibitors using Parsley and Dill seed extracts
Victor V Semenov and M N Semenova
Russian Academy of Sciences, Russia
A nalogs of antimitotic natural products combretastatin A-4 (CA4), podophyllotoxin (PT) and flavanoids were synthesized using
allylpolyalkoxybenzenes from Dill and Parsley seed essential oils. The targeted molecules were evaluated in vivo in a phenotypic
sea urchin embryo assay for antimitotic and microtubule destabilizing activity. Structure activity relationship studies identified
mostly active molecules with polymethoxyphenyl rings as potent antiproliferative agents. The effective threshold concentrations
(EC) resulting in mitotic abnormalities in the sea urchin embryos were 0.25-1 nM. These molecules displayed high cytotoxicity
against a panel of 60 human cancer cell lines including multi drug resistant cells. Cytotoxic effect of tested compounds was attributed
to microtubule destabilization resulted in cell cycle arrest followed by apoptotic cell death. Considering encouraging data from
phenotypic and mechanistic studies, some compounds may prove to be lead candidates for further in vivo studies to assess its potential
as anti-tumor agents.
Biography
V V Semenov is working as the Head of Medicinal Chemistry Laboratory from N D Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences. He is the
author of 25 patents and 280 scientific articles. His research project is on application of nitrogen heterocycles and nitrocompounds in drug design; synthesis of analogs of
natural antimitotics using allylpolyalkoxybenzenes from dill and parsley seed essential oils; biological evaluation of compounds as tubulin modulators in the original sea
urchin embryo assay and; development of chemical library for screening for anticancer, antibacterial, and antifungal activities in cooperation with National Cancer Institute
(Bethesda, USA) and the University of Queensland (Brisbane, Australia).
vs@chemical-block.com
Notes:

July 25-27, 2016

Page 34
Abdul Aziz, J Pharmacogn Nat Prod 2016, 2:3(Suppl)
http://dx.doi.org/10.4172/2472-0992.C1.002

“Scytonemin” pigment from Lyngbya notarisii (Cyanobacteria) may protect human skin from UV
radiations.
Abdul Aziz
University of Dhaka, Bangladesh
L yngbya notarisii (Menegh.) Wille, is characterized by a filament having thick sheath of 10-12 layers around a trichome with yellow-
to reddish-brown pigment “scytonemin”. In Chu 10D medium filaments may attain about 3 cm long and 42 µm wide, including
6.6-14.0 µm diameter sheath when grown under a continuous light flux of 50 µE m-2 s-1 and at a temperature of about 25º C. The
scytonemin produced by trichomes is known to protect cells from UV-A and UV-B radiations and diffuse into sheath layers as the
filament grows, sometimes abundant in the inner layers. The scytonemin is water soluble and continuously oozes out through open
end of a filament after exposure to diurnal cycle having about 950 µE m-2 s-1 light flux during mid day. A sheath containing pure
scytonemin may be separated by maximizing phosphorus concentration in the medium that induces repeated hormogonia formation
from the trichome and their release. The scytonemin impregnated polysaccharide sheath remained may be processed in making
protecting cream reducing the cost of base material and of extraction of the pigment.
Biography
Abdul Aziz has completed his PhD from University of Durham, England in 1985. He is Professor of Botany, published over 100 research papers and served as a Chief Editor,
Bangladesh J. Botany. He has developed large scale Azolla pinnata (used as poultry and fish feeds) production system in ponds round the year; bio-indicator of arsenic
pollution and measuring arsenic in groundwater using Azolla filiculoides; discovered new phenomena of cyanobacterial morphogenesis like differentiation of a hormogonium
or a hair from a single cell depending on availability of PO4-P in the environment; sorted out taxonomic confusions of some cyanobacteria.
dr.aziz.botany@gmail.com
Notes:

July 25-27, 2016

Page 35
Fatma U Afifi-Yazar et al., J Pharmacogn Nat Prod 2016, 2:3(Suppl)
http://dx.doi.org/10.4172/2472-0992.C1.002

Anti-diabetic and anti-obesity potential of selected medicinal plants used in the traditional medicine of
Jordan
Fatma U Afifi-Yazar1, Violet Kasabri1, Entisar Al-Hallaq2 and Simona C Litescu3
1
The University of Jordan, Jordan
2
MSD Animal Health, Jordan
3
National Institute for Biological Sciences-Bucharest, Romania
I n the recent decades complementary/alternative/integrative medicine flourished and lead to the renaissance of nutritional, clinical
and scientific interest in plants’ potential as preventive/therapeutic agents in the management/treatment of chronic diseases.
Worldwide and in Jordan, the prevalence of type-2 diabetes (T2DM) and obesity has reached alarming proportions. In the Jordanian
traditional medicine, Crataegus aronia L. and Adiantum capillus-veneris L. are two of the edible/medicinal plants used for the
treatment of T2DM. The present study gives an overview of anti-diabesity plants of Jordan and discusses the LC-MS evaluation of
the crude extract and in vitro and in vivo evaluation of different biological activities of A. capillus-veneris to evidence its claimed
pharmacological potential. HPLC-MS analyses revealed the presence of ellagic acid (5.48 mg/g), rutin (4.77 mg/g), quercetin-3-
O-glucoside (3.96 mg/g), ferulic acid (3.88 mg/g), gallic acid (3.44 mg/g), caffeic acid (1.55 mg/g), epicatechine (1.34 mg/g) and
quercetine (0.43 mg/g). Hypocholesterolemic efficacy was evaluated in 10-weeks high-cholesterol-diet (HCD) fed rats and compared
to atorvastatin. A. capillus veneris aqueous extract (500mg/kg body weight) decreased highly significantly the total cholesterol (TC)
and low density lipoproteins (LDL) in HCD-fed rats. Additionally, atherogenic index parameter of TC/HDL was normalized in A.
capillus veneris-treated rats. Moreover, the plant extracts’ and some of the identified constituents’ role in modulating gastrointestinal
carbohydrate and lipid digestion and absorption were demonstrated. The results indicate that A. capillus-veneris can be considered a
potential candidate for the management of hypercholesterolemia, obesity and diabetes.
Biography
Fatma U Afifi-Yazar is a Professor of Pharmacognosy and Phytochemistry. She obtained her Doctor of Natural Sciences from ETH Zurich (Switzerland) in 1977. Since 1982,
she works at University of Jordan (UJ), Faculty of Pharmacy where she teaches and supervises MSc and PhD students and holds administrative positions (Dept. Head,
Dean). She has published more than 125 papers in reputed journals and has been serving as an Editorial Board Member of reputed journals. Her research projects were
granted institutionally, governmentally and internationally. She received the “Distinguished Researcher Award” from the UJ for three successive years; 2011, 2012 and 2013.
fatueafi@ju.edu.jo
Notes:

July 25-27, 2016

Page 36
Ekenna Elikee, J Pharmacogn Nat Prod 2016, 2:3(Suppl)
http://dx.doi.org/10.4172/2472-0992.C1.002

Efficacy of detogen-B, a medicinal plant formulation in cancer diseases
Ekenna Elikee
Ekenna Natures Ltd., Nigeria
R ecent global statistics shows that cancers claim more than 8 million lives, while kidney diseases register up to one million cases
annually, with most cases defying even the conventional orthodox medications. Hence, there is always need to explore alternative
approaches to effective cures. Some cases of cancer and kidney failure that have been established in teaching hospitals or licensed
diagnostic laboratories were subjected to our extemporaneous herbal preparations from already researched plants with established
phytochemical properties. Histories of patients’ diets were taken, and the toxicological properties of the food additives noted as
possible risk factors in the disease conditions. Following our treatment protocol, patients with cancers of the liver, cervix, bone,
lungs including cirrhosis, fatty liver and severe kidney diseases have recovered and are now stable. Laboratory evidences from CT
scans, X-ray images, and Bence Jones protein clearance tests were obtained. The first-line drug in these treatments is Detogen-B.
This herbal formulation has passed through sub-acute and sub-chronic toxicity tests conducted by the Nigeria Natural Medicine
Development Agency (NNMDA), under the auspices of the Federal Ministry of Science and Technology, in conjunction with the
Lagos University Teaching Hospital (LUTH). Animal organ tests showed a graphical increase in the mean weights of the liver, pancreas
and lungs showing its activity in the regeneration of tissues. Reduction of LDL and triglycerides levels showed its detoxification and
cardiovascular effects. The Haematological profile shows increase in the levels of WBC, MCH, MCHC and platelets without changes
in the levels of RBC and procalcitonin. All these are indications of the effects of Detogen-B in the treatment of lesions, cancers,
haemangioma and very degenerative disease conditions with immunostimulatory effects.
Biography
Ekenna Elikee is a graduate of the University of Nigeria Nsukka. He is the MD of Ekenna Natures Limited and has devoted more than 10 years after graduation in
understanding the science of phytochemistry and using such knowledge in cure of so many intractable diseases including cancers, kidney/liver diseases and cardiovascular
disorders.
ekennanatures@yahoo.com
Notes:

July 25-27, 2016

Page 37
A V Sirotkin, J Pharmacogn Nat Prod 2016, 2:3(Suppl)
http://dx.doi.org/10.4172/2472-0992.C1.002

Effect of selected food and medical plants and plant molecules on ovarian functions
A V Sirotkin
Constantine the Philosopher University, Slovakia
T he aim of our in vitro and in vivo studies was to examine the potential influence of some medical and food plants and their
constituents on ovarian functions. For this purpose, we have study the influence of green tea, rooibos, ginkgo, flaxseed, yukka
extracts, as well as of plant molecules resveratrol, curcumin, quercetin, daidzein, diosgenin on proliferation, apoptosis, release
of hormones and response to gonadotropins of porcine and rabbit ovarian cells as well as on rabbit fecundity. It was observed,
that green tea, rooibos, ginkgo, flaxseed, extracts, as well as of resveratrol, curcumin, quercetin, daidzein, diosgenin are able to
suppress proliferation, promote apoptosis, to alter the release of steroid hormones and to inhibit the response of cultured ovarian
cells to hormonal stimulators FSH and IGF-I. Yukka extract expressed an opposite effect. Furthermore, feeding of rabbits with yukka
increased their fecundity. These observations suggest potential direct inhibitory influence of food and medical plants green tea,
rooibos, ginkgo, flaxseed on ovarian functions. The similarity in plant and plant constituent effects suggest that the observed plant
effects can be due to presence of curcumin, quercetin, daidzein and diosgenin. The potential anti-reproductive effect of these plants
should be taken into account by their consummation. On the other hand, yukka can be used as a natural stimulator of reproduction
and fecundity.
Biography
A V Sirotkin is working as Professor at the Constantine the Philosopher University, as a Research Scientist at Research Institute of Animal Production in Nitra and as a
Visiting Professor at the King Saud University in Riyadh. He has more than 600 publications including 120 full papers in the international journals. He is a member of editorial
boards of 4 international journals and a recipient of more than 10 national and international awards.
sirotkin@vuzv.sk
Notes:

July 25-27, 2016

Page 38
Ping Zhou, J Pharmacogn Nat Prod 2016, 2:3(Suppl)
http://dx.doi.org/10.4172/2472-0992.C1.002

Isolation and characterization of a hyperbranched proteoglycan from Ganoderma lucidum for anti
diabetes
Ping Zhou
Fudan University, China
I nhibition of protein tyrosine phosphatase 1B (PTP1B) activity has been considered as a promising therapy approach to treat type-
2 diabetes. In this work, a novel PTP1B activity inhibitor, named FYGL (Fudan-Yueyang-G. lucidum), was screened from the
fruiting bodies of Ganoderma lucidum and showed an efficient PTP1B inhibitory potency with IC50=5.12±0.05 μg/mL. The type-2
diabetic animals treated orally by FYGL showed an obvious decrease in the plasma glucose level and comparable with those treated
by metformin, a clinic drug. The toxicity of FYGL is very low. FYGL is a water soluble hyperbranched proteoglycan with molecular
weight (Mη) of 105. In addition, it was also found that FYGL could protect kidney against the renal functional and morphologic
injuries by increasing the activities of antioxidants and inhibiting the accumulation of oxidation. The results indicate that FYGL may
serve as a drug candidate or a health care food for the diabetic therapy and renal functional protection.
Biography
Ping Zhou has completed her BS degree study from Fudan University, China and PhD degree and Post-doctoral studies from The Chinese University of Hong Kong. She
was appointed as a Staff Member at Fudan University and promoted to full Professor in 2005. She has focused on the Research of Biomedicine Materials and developed
new drugs from natural herbs for diabetes treatment. She has published more than 100 scientific papers and got Science and Technology Award (Natural Science), Ministry
of Education of China (2004), Wang Tianjuan Award for Magnetic Resonance Spectroscopy in China (2006) and Shanghai Natural Science Award (2011).
pingzhou@fudan.edu.cn
Notes:

July 25-27, 2016

Page 39
575th Conference
Marine Drugs and

Natural Products
(Day 2)

Tracks 4 & 5
Day 2 July 26, 2016
Track 4: Natural Products Drug Discovery
Track 5: Phytomedicine and Phytochemistry
Session Chair Session Chair
Jun Wu Henry Chen
Jinan University, China Jinan University, China
Title: Unique drug discoveries based on marinopyrrole and novel derivatives
Rongshi Li, University of Nebraska Medical Center, USA
Title: “Seed to patient”: Role in clinically proven natural medicines
Dilip Ghosh, Soho Flordis International, Australia
Title: Glucosyl terpenates from the dried fruits of Prunus domestica L
Shin-ichi Kayano, Kio University, Japan
Title: Flavones and flavonoids from wild Aquilaria sinensis leaves: In vitro bioactivities and in vivo regulation
against impairment of the plant
Henru Chen, Jinan University, China
Title: Chemical diversity of limonoids from mangrove plants
Jun Wu, Jinan University, China
Title: Bufadienolides with cytotoxic activity from the skins of Bufo bufo gargarizans
Lei Wang, Jinan University, China
Title: Kukoamine B Ameliorates rodent polymicrobial sepsis via simultaneous blockade of primary PAMPs
and induced inflammatory disorderss
Xin Liu, Third Military Medical University, China

Page 44
Rongshi Li, J Pharmacogn Nat Prod 2016, 2:3(Suppl)
http://dx.doi.org/10.4172/2472-0992.C1.002

Unique drug discoveries based on marinopyrrole and novel derivatives
Rongshi Li
University of Nebraska Medical Center, USA
N atural products and their derivatives account for about half of the New Chemical Entities (NCEs) for drug discovery to treat
human diseases. For example, 78 of the 118 small-molecule NCEs identified for antibacterial agents were from either natural
products or their derivatives during 30 years between 1981 and 2010. For anticancer drug discoveries, 85 of the 175 small molecules,
for 70-year span from 1940 to 2010, were from natural products, their derivatives, metabolites and mimics. To date, natural products
have provided the most successful supply of drug leads. In this talk, a case study of marinopyrrole and its novel derivatives as potential
antibiotic and anticancer agents will be presented. The design, synthesis and optimization of these novel derivatives to achieve
improved physicochemical and drug-like properties and potent biological activities and high selectivity with low toxicity will be
discussed.
Biography
Rongshi Li is a Professor of Chemistry and Medicinal Chemistry in the Department of Pharmaceutical Sciences, College of Pharmacy, of University of Nebraska Medical
Center, USA. He spent 14 years in industry and advanced from Scientist to Senior Vice President after his Postdoctoral training at University of California, San Francisco.
He began his academic career in 2008 at Moffitt Cancer Center, Tampa, Florida. In 2013, he was recruited at University of Nebraska Medical Center. Since 2008, he has
published over 20 peer-reviewed articles, filed and published 30 US and PCT patents, edited one book and delivered over 30 invited talks.
rongshi.li@unmc.edu
Notes:

July 25-27, 2016

Page 45
Dilip Ghosh, J Pharmacogn Nat Prod 2016, 2:3(Suppl)
http://dx.doi.org/10.4172/2472-0992.C1.002

“Seed to patient”: Role in clinically proven natural medicines
Dilip Ghosh
Soho Flordis International & NutriConnect, Australia
S tandardized extracts are processed products, where some specific and known components, recognized to contribute more than
others to the therapeutic activity, are adjusted to a given amount, within the acceptable tolerance. Standardization is achieved by
adjustment of natural substance/herbal preparation with excipients or by blending batches of natural substance/herbal preparations.
All the other components are still present in the extract, because the action of the plant may result from the synergistic activity of
several constituents. According to the concept of phytoequivalence, a chemical profile, such as a chromatographic fingerprint, for a
natural product should be constructed and compare with the profile of a clinically proven reference product. Plants are highly variable
by nature and it is a common experience that batches of medicinal plants with similar specifications, as species and part of plant, may
have quite different chemical compositions. From many reasons, it is clear that the “generic” concept which is valid for conventional
medicines is mainly invalid for “Specifically Clinically Proven” natural medicines. Increasing the quantity and quality of clinical and
scientific information on herbal medicinal products will reduce uncertainty in assessment and greatly contribute to decision making
related to hazard and risk.
Biography
Dilip Ghosh has received his PhD in Biomedical Science from University of Calcutta, India. Previously, he held positions in Organon (India) Ltd.; HortResearch, New
Zealand; USDA-ARS, HNRCA at Tufts University, Boston; The Smart Foods Centre, & Neptune Bio-Innovation Pty. Ltd., Australia. He is an international speaker, facilitator
and author. He is a fellow of American College of Nutrition, professional member of AIFST, and also he is the Editorial Board Member of several journals. He has published
more than 70 papers in peer reviewed journals, numerous articles in food and nutrition magazines and 4 books under CRC Press, USA.
dilip.ghosh@sfihealth.com
dghosh@optusnet.com.au
Notes:

July 25-27, 2016

Page 46
Shin-ichi Kayano, J Pharmacogn Nat Prod 2016, 2:3(Suppl)
http://dx.doi.org/10.4172/2472-0992.C1.002

Glucosyl terpenates from the dried fruits of Prunus domestica L
Shin-ichi Kayano
Kio University, Japan
T he chemical structures of 9 compounds isolated from the dried fruits of Prunus domestica L., were elucidated on the basis of NMR
and MS analyses. Each isolated compound was determined to be scopolin (1), (3-O-cis-p-coumaroyl-β-D-fructofuranosyl)-
(2→1)-α-D-glucopyranoside (2), 1S-(4-β-D-glucopyranosyl-3-methoxyphenyl)-2R-[4-(3-hydroxypropyl)-2-methoxyphenoxy]-1,3-
propanediol (3), β-D-glucopyranosyl 9-carboxy-8-hydroxy-2,7-dimethyl-2E,4E-nonadienate (4), β-D-glucopyranosyl 7-carboxy-
2-methyl-2E,4E-octadienate (5), 8-hydroxy-2,7-dimethyl-2E,4E-decadienedioic acid 1-β-D-glucopyranyl ester 10-methyl ester
(6), (3-O-trans-p-coumaroyl-β-D-fructofuranosyl)-(2→1)-α-D-glucopyranoside (7), β-D-glucopyranosyl cinnamate (8), and
2,7-dimethyl-2E, 4E-octadienedioic acid (9), respectively. Compounds 2, 3, 7, 8, and 9 were isolated from Prunus domestica L., for
the first time and compounds 4, 5 and 6 were novel glucosyl terpenates. On the basis of the chemical structures of some isolated
compounds from Prunus domestica L., a possible biosynthesis pathway of them was also proposed.
Biography
Shin-ichi Kayano has graduated from Department of Food Science and Nutrition, Osaka City University, Japan, in 1985 and he has worked as a Senior Researcher at
Research Institute, Miki Corporation, Japan, until 2004. He was awarded a PhD in Science in 2004 from Osaka City University, Japan, under the supervision of Professor
Nobuji Nakatani. The same year, he has changed his job in Kio University and currently working as a Professor since 2009.
s.kayano@kio.ac.jp
Notes:

July 25-27, 2016

Page 47
Heru Chen et al., J Pharmacogn Nat Prod 2016, 2:3(Suppl)
http://dx.doi.org/10.4172/2472-0992.C1.002

Flavones and flavonoids from wild Aquilaria sinensis leaves: In vitro bioactivities and in vivo regulation
against impairment of the plant
Heru Chen and Maoxun Shang
Jinan University, China
A quilaria sinensis is the famous traditional Chinese medicinal plant in Guangdong, south China. The well-known medicinal part
is the resin from Aquilaria sinensis, which is the response of the plant against external impairment. Therefore, the formation
of resin is an incidental and lengthy process. This makes the resin very scarce and cherish. However, Aquilaria sinensis leaves are
abundant and reproducible. The current report focused on the isolation of flavones and flavonoids, the in vitro bioactivities, and
in vivo regulation against external impairment of the plant. By applying means of solvent partition and various kinds of column
chromatography including silica gel, Sephadex LH-20, C-18 reverse phase HPLC, and HSCCC, on the basis of physical properties
and spectra evidences, 12 flavones and flavonoids have been identified. The scavenging effects on nitrite of these compounds were
determined. Compound Y1 (mangiferin) and Y2 (2-O-α-L-rhamnopyranosyl-4, 6, 4’-trihydroxybenzo-phenone) were identified as
the two most active compounds with scavenging rate 29.43%±0.74% and 24.56%±0.77% at the conditions of pH 3, 37, and 30 min
duration, respectively. Furthermore, the in vitro cancer cell growth inhibition activities of 12 compounds were evaluated by MTT
method, respectively. 7 of them were demonstrated good inhibitory activity against DU145PC-3, and HepG2 cancer cell lines,
respectively. It was indicated preliminarily that these compounds may be used as the candidates for developing anti-cancer drug.
Most interestingly, as one secondary metabolite, injection of genkwanin with moderate concentration (0.5mM) exhibited significant
protective effect against external mechanical and chemical injuries to Aquilaria sinensis plants. Five differential expressed proteins
including Armadillo repeat-containing kinesin-like protein-1 (PT/ST promoter) and Tetrathionate response regulatory protein TtrR
(TC/MG promoter) were identified using differential proteomics analysis method, and the regulation mechanism of genkwanin
against the external impairments of Aquilaria sinensis plants has been demonstrated preliminarily. It is suggested that genkwanin play
an important role in defense of external plant impairment.
Biography
Heru Chen has completed his PhD from the Hong Kong University of Science and Technology with Prof. Richard K Haynes as supervisor. He got his Post-doctoral training
with Prof. Dr. Peter W. Schiller in the Clinical Research Institute of Montreal, Canada. Then he worked as a Visiting Scholar in Wuppertal University, Germany. Since March
2008, he has been a full-time Professor and Research Director in the Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University,
China. He has published more than 80 papers in reputed journals and serving as Editorial Board Members of several reputed journals.
thrchen@jnu.edu.cn
Notes:

July 25-27, 2016

Page 48
Jun Wu et al., J Pharmacogn Nat Prod 2016, 2:3(Suppl)
http://dx.doi.org/10.4172/2472-0992.C1.002

Chemical diversity of limonoids from Mangrove plants
Jun Wu and Li Shen
M angrove plants are a large group of different salt tolerant plants growing in tropical and subtropical intertidal estuarine zones.
Limonoids, which have been found mainly in plants of the families Meliaceae, Rutaceae, and Simaroubaceae, are modified
triterpene derivatives originating from a precursor with a 4,4,8-trimethyl-17-furanylsteroid skeleton displaying four usually highly
oxidized rings (designated as A, B, C, and D) in the intact triterpene backbone. The mangroves of the genus Xylocarpus are known
to produce a variety of antifeedant limonoids, especially mexicanolides and phragmalins. During the recent ten years, my team has
identified more than 150 new limonoids from mangrove plants of the genus Xylocarpus collected in south China, India and Thailand.
More than 30 limonoids, such as xylogranatins F-R, andhraxylocarpins A-E, and thaixylomolins A-C, are compounds with new
skeletons. These limonoids exhibited plentiful chemical diversity. It is suggested that environmental factors might play the leading
role in the generation of structural diversity of limonoids from those mangroves. The study also demonstrates that mangroves of the
genus Xylocarpus are a rich source for the production of limonoids with novel carbon frameworks.
Biography
Jun Wu has completed his PhD from Peking University in 2001 and won the National Natural Science Foundation of China award for Distinguished Young Scholars in 2011.
He is the Director of Marine Drugs Research Center, Jinan University. His research interests focus around bioactive natural products from mangrove plants, mangrove
endophytic fungi and marine dinoflagellates. His group has identified more than 100 new limonoids from mangrove plants of the genus Xylocarpus collected in India and
Thailand. Some limonoids exhibited antifeedant, insecticidal, anti-tumor and anti-infective activities. He has published more than 120 papers which have been cited over
900 times.
wwujun68@163.com
Notes:

July 25-27, 2016

Page 49
Lei Wang et al., J Pharmacogn Nat Prod 2016, 2:3(Suppl)
http://dx.doi.org/10.4172/2472-0992.C1.002

Bufadienolides with cytotoxic activity from the skins of Bufo bufo gargarizans
Lei Wang, Bao-Jing Li, Dong-Mei Zhang, Ren-Wang Jiang and Wen-Cai Ye
T he toad Bufo bufo gargarizans Cantor is distributed in most regions of China. The dried skin of B. bufo gargarizans has been
used as a traditional Chinese medicine (‘Chan-Pi’ in Chinese) for the treatment of tumor, carbuncle, scrofula and heart failure.
Cinobufacini (Hua-Chan-Su) injection, prepared from the skins of B. bufo gargarizans, has been widely used in clinical cancer therapy
in China for a long time. Pharmacological studies revealed that this injection has antitumor and anti-hepatitis B virus activities. The
bioactive constituents of cinobufacini injection were considered to be bufadienolides. Bufadienolides are typically C-24 steroid with
a characteristic α-pyrone ring at C-17 position, which attracted much attention of pharmacologists due to their significant antitumor
activities. Recent research on the action mechanism revealed that bufalin could activate ClC-3 Cl− channel and induce apoptosis
through the PI3K/Akt/mTOR pathway. Although about forty bufadienolides had been isolated from the skins of B. bufo gargarizans,
further investigation to exploit other bioactive compounds and trace constituents of this commercial toad skin are necessary. As a
part of our work to discover new potential anti-tumor agents, twelve new bufadienolides (1-12) and fourteen known ones (13-26)
were isolated from the skins of B. bufo gargarizans. Among them, compound 1 was an unusual bufadienolide with 3,19-epoxy moiety.
Compounds 2-3 and 4 were rare bufadienolides possessing 10-H atom and 10-carboxyl unit, respectively. In addition, the cytotoxic
effects of the isolated compounds against HepG2, A549 and HeLa cell lines were evaluated. Six new compounds (2, 3, 5, 6, 10, 12)
displayed significant anti-proliferative activities with IC50 values ranging from 0.049 to 1.856 μM. Arenobufagin (24) exhibited the
most potent cytotoxic activity with IC50 value 0.011 μM.
Biography
Lei Wang has completed his PhD from China Pharmaceutical University and Post-doctoral studies from Jinan University. He has published more than 52 papers in reputed
journals and has applied for 5 patents.
cpuwanglei@126.com
Notes:

July 25-27, 2016

Page 50
Xin Liu, J Pharmacogn Nat Prod 2016, 2:3(Suppl)
http://dx.doi.org/10.4172/2472-0992.C1.002

Kukoamine B ameliorates rodent polymicrobial sepsis via simultaneous blockade of primary PAMPs
and induced inflammatory disorders
Xin Liu
Third Military Medical University, China
K ukoamine B (KB) is an alkaloid compound isolated from the traditional Chinese herb Cortex Lycii. KB has been identified as a
novel dual antagonist for LPS and CpG DNA; two major pathogens associated molecular patterns (PAMPs) for triggering sepsis.
However, its inhibitory effects against LPS and CpG DNA in vivo and the potential therapeutic implications in treating sepsis need
to be well elucidated. In this study, the impact of KB on survivals of different rodent sepsis models was analyzed. Organ distribution
and clearance of LPS and CpG DNA in the presence of KB were detected. The antagonistic effects of KB against LPS and CpG
DNA, including inflammatory associated cytokines production, coagulation parameters, major organ functions were evaluated in
rats CLP model. We found that KB could bind bacterial LPS from different bacterial origins and inhibit the induced release of pro
inflammatory cytokines. KB attenuates the activity of LPS and CpG DNA in vivo, accelerates their circulatory clearance and increases
liver uptake. KB effectively improves the survival of different murine sepsis models. KB also ameliorates the production of pro and
anti-inflammatory cytokines and serum biomarkers of sepsis in CLP rats, KB efficacy in reversing their acidosis state, suppressing
DIC and organ dysfunctions and limiting acute lung injuries secondary to sepsis. Overall, KB is effective in simultaneously blockade
of LPS and CpG DNA in vivo and improves the outcomes in rodent sepsis models. KB may become a promising candidate drug for
the treatment of sepsis.
Biography
Xin Liu has received his PhD in Southwest Hospital, Third Military Medical University, China in 2010. He currently serves as an Associate Professor in the same institute.
His research interest lies mainly on investigating therapeutic intervention of sepsis by means of natural products. He has co-operated with his fellow researchers to screen
more than 140 traditional Chinese herbs and identify a couple of novel anti-sepsis compounds.
triplestars@163.com
Notes:

July 25-27, 2016

Page 51
575th Conference
Marine Drugs and

Natural Products
Young Researchers Forum
(Day 2)

Hong Ngoc Thuy Pham et al., J Pharmacogn Nat Prod 2016, 2:3(Suppl)
http://dx.doi.org/10.4172/2472-0992.C1.002

Optimization of ultrasound assisted extraction of total phenolic compounds and antioxidant activity
from Helicteres hirsuta Lour
Hong Ngoc Thuy Pham1,2, Quan Van Vuong1, Michael C Bowyer1 and Christopher J Scarlett1
1
University of Newcastle, Australia
2
Nha Trang University, Vietnam
H elicteres hirsuta Lour (H. hirsuta L) is wildly distributed in Southeast Asian countries and has been used as a traditional medicine
for the treatment of various ailments such as malaria and diabetes. This study aimed to optimize ultrasound assisted extraction
conditions for retaining maximum yield of bioactive compounds and antioxidant capacity from H. hirsuta L. Response surface
methodology (RSM) with central composite design (CCD) was employed to design experiments for assessing the effect of ultrasonic
temperature (40-60ºC), ultrasonic time (15-35 min), ultrasonic power (60-100% or 150-250 W), sample-to-solvent ratio (1-6 g/100
mL) and methanol concentration (0-50%) on the yields of total phenolic content, total flavonoid content and antioxidant activity
of H. hirsuta L extract. The results showed that ultrasonic temperature, sample-to-solvent ratio and methanol concentration had a
significant influence on the extraction efficiency of phenolics, flavonoids and antioxidant capacity. The optimal extraction conditions
were ultrasonic temperature of 60ºC, ultrasonic time of 25 min, ultrasonic power of 150W, sample-to-solvent ratio of 3:100 g/mL,
with the solvent being 40% (v/v) methanol. The actual values obtained under these conditions were 15.97 mg GAE/g of phenolics,
16.42 mg CE/g of flavonoids and 13.34 g/100 g of extractable solids. The highest values of the antioxidant assays (DPPH, ABTS and
FRAP) were also observed under these conditions, with the exception of CUPRAC (obtaining 87% maximum value). These optimal
extraction conditions can be applied to produce powdered crude extract for further isolation and purification of individual bioactive
compounds for their potential use in the nutraceutical and pharmaceutical industries.
Biography
Hong Ngoc Thuy Pham has completed her BSc in Food Technology in 2004 and completed her Master’s degree in Post-harvest Technology from Nha Trang University,
Vietnam in 2009. She is currently a Lecturer of Nha Trang University, Vietnam and a PhD student at the University of Newcastle, Australia. She has published 7 papers in
domestic and international journals. She is presently working on the research project entitled: “Extraction of anticancer compounds from selected medicinal plants as novel
agents against pancreatic cancer cells” at the University of Newcastle, Australia.
HongNgocThuy.Pham@uon.edu.au
Notes:

July 25-27, 2016

Page 53
Haema Thevanayagam, J Pharmacogn Nat Prod 2016, 2:3(Suppl)
http://dx.doi.org/10.4172/2472-0992.C1.002

Carrageenan and its potential use as a photoprotective agent: Anti-inflammatory and apoptotic cell death
Haema Thevanayagam
International Medical University, Malaysia
T he use of marine resources for photoprotection is heightened at present. Carrageenan, the polysaccharide from red algae which
is used in food and medicine is also a widely used excipient in cosmetics and skincare products. Carrageenan has shown to
have prospective photoprotective effect against UVB irradiation on immortalized normal human keratinocyte (HaCaT) cells in
vitro by preventing excessive cell death, exhibiting antioxidant and free radical scavenging effect while stimulating the skin’s natural
antioxidant enzymes. In this study, we aim to evaluate the anti-inflammatory action of iota (ι), kappa (κ) and lambda (λ) carrageenan
and the favor of apoptotic cell death over necrotic cell death after UVB exposure in HaCaT cells. Results indicated that carrageenan
pretreated cells had significantly (p<0.05) reduced levels of interleukin-1α (IL-1α) by 41.67-100% in comparison to the cells without
treatment where IL-1α increased 22.84-61.46% after irradiation. Successively, carrageenan pretreated cells had lower occurrence of
cell death and the nature of cell death in these cells showed a higher percentage (6.16-29.06%) of apoptotic cell death rather than
necrotic. At 100 mJ per cm² an increase of 21.79% of necrotic cell death was observed in the untreated cells. This signifies the potential
use of carrageenan as an anti-inflammatory agent along with its promising antioxidant property and its ability to reduce necrotic cell
death thus preventing excessive inflammatory reactions. The combination of these properties and the additional value of carrageenan
deduced from this study would enhance the performance of skin care regimes and the prospective role in photoprotection.
Biography
Haema Thevanayagam has completed her A-Levels in INTI International College and her Tertiary Education at Management and Science University in Bachelor of
Biomedical Science. She then completed her Master of Science Degree (MSc Medical and Health Sciences) in International Medical University and graduated in 2013. She
is currently pursuing her PhD in the same field concentrating on marine derivatives, photo aging and skin cancer.
roja59714@yahoo.com
Notes:

July 25-27, 2016

Page 54
Irma Antasionasti et al., J Pharmacogn Nat Prod 2016, 2:3(Suppl)
http://dx.doi.org/10.4172/2472-0992.C1.002

Determination of Antioxidant Activity and Identification of Active Compounds From the Ethyl Acetate
Extract of Avocado Peel
Irma Antasionasti, Sugeng Riyanto and Abdul Rohman
Gadjah Mada University, Indonesia
A research had been conducted to test antioxidant activity and to identify the active compounds of antioxidant from the ethyl acetate
extract of avocado peel. The research aims to determine antioxidant activity of the ethyl acetate extract from avocado peel using
in vitro method based on DPPH and ABTS radical scavenging, reducing power of iron (III), total phenolic and flavonoid content. To
identify the structure of the active compound of antioxidants from the ethyl acetate extract of avocado peel, FTIR spectroscopy and
GC-MS are used. Ethyl acetate extract of avocado peel had antioxidant activity with IC50 values of DPPH, ABTS radical scavenging,
reducing power, total phenolic and flavonoid content are 18,387±0,022 µg/mL, 5,487±0,216 µg/mL, 179,748±3,057 mg of ascorbic
acid/gram of extract, 13,178±0,809% w/w EGA and 39,835±0,607% w/w ER, respectively. Isolate compound of the ethyl acetate
extract was identification to be 1,2,4-trihidroksiheptadek-16-yne-18-ene. Avocado peel can be used as natural antioxidant source to
prevent diseases associated with free radical.
Biography
Irma Antasionasti has completed her undergraduate program from Chemistry Education at Halu Oleo University, Kendari, Indonesia. She is currently doing her MSc
Program in Faculty of Pharmacy Gadjah Mada University, Yogyakarta, Indonesia. She receives scholarship from The Indonesia Endowment Fund for Education, Ministry of
the Finance Republic of Indonesia. She is working on antioxidant activity and identification of active compounds from natural products.
irmaantasionasti07@gmail.com
Notes:

July 25-27, 2016

Page 55
575th Conference
Marine Drugs and

Natural Products
(Day 3)

Tracks 8, 9 & 10
Day 3 July 27, 2016
Track 8: Marine: The Ultimate Source of Bioactives and Drug Metabolites
Track 9: Marine Biotechnology
Track 10: Marine Natural Products Drug Discovery
Session Chair
Laurent Lebrun
University of Rouen, France
Title: Marine based ingredients for natural cosmeceuticals
Jeanine B Downie, Overlook and Mountainside Hospitals, USA
Title: Seaweed extracts as potential source of new antiviral agents for bio-based cleaning wipes and filters
Laurent Lebrun, University of Rouen, France
Title: Cholinesterase inhibitory and protective effects of algal compounds against neurotoxicity and
neuroinflammation
Gan Sook Yee, International Medical University, Malaysia
Title: Fortune from the red Carrageenan: A discovery for photoprotection
Shar Mariam Mohamed, International Medical University, Malaysia
Title: Exploration of potential Indonesian seaweeds
Noer Kasanah, Universitas Gadjah Mada Yogyakarta, Indonesia
Title: The potential of Malaysian seaweeds as the source of antifungal compounds for application in the
oil palm industry
Zetty Norhana Balia Yusof, Universiti Putra Malaysia, Malaysia

Page 57
Jeanine B Downie, J Pharmacogn Nat Prod 2016, 2:3(Suppl)
http://dx.doi.org/10.4172/2472-0992.C1.002

Marine based ingredients for natural cosmeceuticals
Jeanine B Downie
Overlook and Mountainside Hospitals, USA
M arine compounds are currently used in antibiotics derived from fungi. These include compounds from a sponge that have
been found to be useful in treating cancer. One specific neurotoxin from a sea snail has been determined to be as strong and
effective as morphine. They are ubiquitous as unicellular organisms make up more than 95% of the living organisms in the ocean.
These microorganisms grow where no other life forms can, very deep in the ocean. Logically, if they can survive in the ocean depths,
they may be helpful in protecting our skin from environmental stressors. Ocean organisms can be sun burned and it appears that they
have adapted and developed compounds/mechanisms that help repair damage from the environment. Preserving algae is the best way
to keep the potency of the seaweed. This is done by cold extraction or freeze-drying. We will discuss promising marine compounds
for cosmeceutical use and the new hyaluronic acid moisturizer, HA 5 which has marine micro- organism polysaccharide peptide
complex in it.
Biography
Jeanine B Downie MD, MA is a board-certified Dermatologist and the Director of Image Dermatology in Montclair, NJ. She has published 37 articles in peer-reviewed
journals and lectures nationally and internationally on Cosmetic Dermatology. She consults for the top-tier pharmaceutical companies and does cutting edge clinical trials
for them as well. She is a medical television contributor that frequently appears on The Today Show, The Dr. Oz Show, Good Morning America and The View among other
shows. She has been honored repeatedly by Castle Connolly as one of New York Metropolitan’s Top Doctors. Her first book, Beautiful Skin of Color is a comprehensive skin
care guide for Asian, Olive and Dark Skin (2004).
jeaninedownie@yahoo.com
Notes:

July 25-27, 2016

Page 58
Laurent Lebrun, J Pharmacogn Nat Prod 2016, 2:3(Suppl)
http://dx.doi.org/10.4172/2472-0992.C1.002

Seaweed extracts as potential source of new antiviral agents for bio-based cleaning wipes and filters
Laurent Lebrun
University of Rouen, France
D uring viral epidemics such as influenza, Ebola or SARS many states are unable to afford effective but expensive filtering facepiece
respirators (FFP3 or N95 type). Unefficient alternative respiratory protective equipments such as cotton fabrics and medical
masks are often used. Several methods have been recently developed for producing low-cost virucidal filters suitable for respiratory
protective masks. The use of natural biocide molecules could make a contribution to introduce antimicrobial properties inside
cellulose filters. Surface cleaning represents also an essential disinfection procedure for virus elimination from contaminated surfaces.
The cleaning by modified wipes containing antiviral compounds is also of major interest. The present work reports the preparation of
new antiviral cleaning wipes and filters using seaweeds extracts as antiviral agents. 30 seaweeds extracts containing ulvans, fucoidans,
alginates, pectins or polyphenols were tested. The antiviral experiments were first performed on suspensions of T4D bacteriophage
of Escherichia coli. Only polyphenols revealed antiviral activity. All polyphenol-grafted cellulose layers exhibited a large improvement
in the reduction of the viral concentration (5-log after 20 min). Hence, these materials could be used as virucidal wipes for the virus
elimination from contaminated surfaces. Virus filtration experiments were performed by spraying a suspension of bacteriophage
through modified layers. The virus reduction was improved 10-fold for monolayer and 4-fold for bi-layers. Finally, two layers were
placed inside a commercial medical mask in place of its cellulose layer. The virus reduction was improved 12-fold compared with the
original mask. Based on these results, a significant improvement over conventional commercial medical masks was obtained.
Biography
Laurent Lebrun has completed his PhD in Physicochemistry in 1993 from Rouen University. He is currently an Assistant Professor. He has published 60 peer-reviewed
publications and is Co-Inventor of one patent and two standards. His main research interest is concerned with polymers (synthesis and characterization), membranes,
barrier materials and the use of polymers for the improvement of the environment. His application fields are packaging materials, gas separation and wastewater treatments
and biocomposites.
Laurent.lebrun@univ-rouen.fr
Notes:

July 25-27, 2016

Page 59
Gan Sook Yee, J Pharmacogn Nat Prod 2016, 2:3(Suppl)
http://dx.doi.org/10.4172/2472-0992.C1.002

Cholinesterase inhibitory and protective effects of algal compounds against neurotoxicity and neuroinflammation
Gan Sook Yee
F ucosterol, pheophytin A and pheophytin A isomer isolated from brown seaweeds such as Padina ornata and Sargassum polycystum
as well as caulerpin isolated from the green seaweed, Caulerpa racemosa were assayed for their cholinesterase inhibitory activities
and their neuroprotective effects on amyloid β1-42 (Aβ1-42) / glutamate induced SH-SY5Y cells. Their anti-neuroinflammatory effects
were also determined by measuring the levels of cytokines and pro-inflammatory mediators in lipopolysaccharide (LPS)-stimulated
C8-B4 microglia cells. All four algal compounds exhibited inhibitory activities on acetylcholinesterase and butyrylcholinesterase in
a dose dependent manner. They significantly increased the viability of Aβ1-42 / glutamate induced SH-SY5Y cells but suppressed the
expression of TNF-α, IL-1β, IL-6, NO and PGE2 in LPS-stimulated C8-B4 cells. The four algal compounds showed dual cholinesterase
inhibition and neuroprotective effects against Aβ1-42/ glutamate/ LPS suggesting possible applications for the prevention of Alzheimer’s
disease.
Biography
Gan Sook Yee has completed her PhD in Algal Biotechnology in 2005 from University Malaya, Malaysia. Currently, she is the Head of Life Sciences department under the
School of Pharmacy at the International Medical University. Her main research interest is in the area of Algal Biotechnology (genetic engineering, algal transcriptomics
and bioactives). She is also trained in Molecular Biology and has involved in gene expression studies and miRNA research in nasopharyngeal carcinoma. She is presently
involved in the study of algal bioactives for neurodegenerative disorders.
sookyee_gan@imu.edu.my
Notes:

July 25-27, 2016

Page 60
Shar Mariam Mohamed, J Pharmacogn Nat Prod 2016, 2:3(Suppl)
http://dx.doi.org/10.4172/2472-0992.C1.002

Fortune from the red carrageenan: A discovery for photoprotection
Shar Mariam Mohamed
P hoto protection against ultraviolet radiation is a major concern worldwide and the best protection agent has yet to be found.
Carrageenan is a polysaccharide extracted from the red seaweed mostly of the genera Chondrus, Eucheuma, Gigartina and Iridae
is used as a gelling and thickening agent in the food industry, medicines and as an excipient in cosmetics. Considerably, carrageenan
is believed to have prospective photo protective properties. In the current study the cytotoxicity, photo protection and Rat Sarcoma
(RAS)-Rapidly Accelerated Fibrosarcoma (RAF) gene mutation of iota, kappa carrageenan and their synergism with vitamin E was
evaluated against UVB induced immortalized normal human keratinocyte (HaCaT) cells. MTT results for cytotoxicity and photo
protection indicated that carrageenan was not toxic to cells if used in concentration lower that 200 µg/ml with CD50 values of 80 and
90 µg/ml for iota and its synergism with vitamin E and 132 and 155 µg/ml for kappa and its combination with vitamin E respectively.
Cells pre-treated with carrageenan exhibited significantly (p<0.05) higher cell viability compared to the cells without treatment by
3.53-27.73% after 100 mJ/cm² and 11.08-45.17% after 300 mJ/cm² UVB fluence. The incident of RAS mutation using the RAS-RAF
pathway somatic mutation assay was lower in cells treated with carrageenan compared to those without. Collectively results suggest
the potential use of carrageenan as a photo protective agent. An added value of carrageenan rather than being only an excipient could
be deduced from this study which is worthwhile for further exploration on its other mechanisms that promises photo protection.
Biography
Shar Mariam Mohamed is currently the Head of Human Biology Department under the School of Medicine. She is one of the pioneer team members who developed the
Medical Biotechnology Programme dated back in 2005-2006. She has been extensively involved in curriculum design and reviewed the programme. She has introduced
and developed the Enterprise Management module which has now become the unique feature of IMU’s Medical Biotechnology Program. Her area of expertise includes cell
and human physiology. Her research career has focused on natural products and their anti-cancer potential and properties. The two main areas of her research interest are
the studies on the effects of carrageenan, a polysaccharides from red seaweed on human keratinocytes and on the anticancer activities of gram positive bacterium Bacillus
thuringiensis (BT18 strain) against leukaemic cell line CEM-SS. The study on carrageenan revealed its role as a photoprotective agent against UVB-induced cell killing,
hence indicating its potential in minimizing skin cancer. The study has won an international recognition (Dr Martin Baker Memorial Prize, SCCANZ Skin Cancer Conference,
Australia) in 2011. She has been a member of the Working Group for setting Malaysian Standards on Safety in Laboratories (SIRIM) – Microbiological since 2008. She is
an Invited speaker at the 4th World Congress of Marine Biotechnology, Dalian China during 16-18 October 2014; Panel member of drafting the National Act On “Biorisks/
Biothreats: Bridging Science and Security” held in Penang Malaysia (2014) and; Career coach at the Biocareer 2014 in Conjunction With Biomalaysia & Bioeconomy Asia
Pacific 2014 held at the University of Malaya, organized by the Malaysian Biotechnology Corporation.
sharmariam_mhd@imu.edu.my
Notes:

July 25-27, 2016

Page 61
Noer Kasanah et al., J Pharmacogn Nat Prod 2016, 2:3(Suppl)
http://dx.doi.org/10.4172/2472-0992.C1.002

Exploration of potential Indonesian seaweeds
Noer Kasanah1, Triyanto1, Yuliana Saloso2, Wellem Turupadang2, Husnul Khotimah1, Muhamad Akbar Hakim1, Ajeng Diyan Novitasari1 and Aji Nugroho1
1
Universitas Gadjah Mada, Indonesia
2
Universitas Nusa Cendana, Indonesia
S eaweeds and their extracts have been studied for decades as novel sources a variety of compounds and some of them have been
reported to possess biological activity of potential medicinal value. Seaweeds are great potential producer of secondary metabolites
that responsible for bioactivities which have commercial application in pharmaceutical, medical, cosmetic, nutraceutical and
agricultural industries. In order to fully explore and exploit the value and potential of local seaweed we initiated research project on
Indonesian seaweeds since 2012. The objectives of research program were (1) to explore the indigenous various seaweeds, collected
from parts of Indonesia mainly in Yogyakarta Coastal region and East Nusa Tenggara, (2) to study and determine chemical constituent
and screen for the potential as edible seaweed (sea vegetables), anti oxidant, antibacterial agents and sulfated polysaccharide. More
than 100 seaweeds were collected and screened for antibacterial, antioxidant, sulfated polysaccharides and toxicity. We followed
bioassay guided isolation to determine bioactive compounds. Our results showed that potential of Indonesian seaweeds are very
promising. We are going to discuss in details apesies, chemical diversity and bioactivity in the presentation.
Biography
Noer Kasanah has completed her PhD from School of Pharmacy, University of Mississippi (2008) and Post-doctoral studies from College of Pharmacy, Oregon State
University (2010). She is an Assistant Professor and Principal Investigator of Marine Biotechnology Research Group, Department of Fisheries, Faculty of Agriculture,
Universitas Gadjah Mada Yogyakarta since 2011.
noer.kasanah@ugm.ac.id
Notes:

July 25-27, 2016

Page 62
Zetty Norhana Balia Yusof, J Pharmacogn Nat Prod 2016, 2:3(Suppl)
http://dx.doi.org/10.4172/2472-0992.C1.002

The potential of Malaysian seaweeds as the source of antifungal compounds for application in the oil
palm industry
Zetty Norhana Balia Yusof
Universiti Putra Malaysia, Malaysia
O il palm (Elaies guineensis) is a major contributor of the world’s edible vegetable oil and also a potential source of biodiesel in
Malaysia. However, it is confronted with a serious disease caused by fungi. Ganoderma boninense, a plant pathogenic fungus
was recognized as the main factor that causes a disease which will affect the production of oil palm products and also causes death in
palms. To date, there is no efficient strategy for early detection or control of this disease just yet. Fungicide and chemicals have been
utilized as the main control but they are harmful for the environment and human health. Alternative disease control utilizing organic
sources such as microbes and plants have been postulated to be having high potential. Seaweeds or also known as macroscopic, multi-
cellular, marine algae have been known to possess various natural compounds with antifungal activities. Seaweeds are abundant in
Malaysia and most of their potential and advantages are under-explored. The utilization of seaweeds will be a more natural way of
controlling the disease without harmful effects to the environment as well as human. This project aimed at discovering potential local
seaweeds which possess useful antifungal characteristics and also at elucidating their bioactive compounds with inhibitory activity
against oil palm disease-causing fungus, G. boninense. Crude extracts were recovered from selected seaweeds of Malaysia and tested
for their antifungal activities. Phytochemical analyses were carried out to identify the potential bioactive compounds for antifungal
activities. This project is hoped to be the solution for the never ending hunt for the best disease-control mechanism of diseases caused
by fungus in oil palm.
Biography
Zetty Norhana Balia Yusof is working as a senior lecturer in the department of Biochemistry, as a Faculty of biotechnology and Biomolecular Sciences. Her research interest
are Thiamine (vitamin B1) biosynthesis pathway in plants, specifically in oil palm (Elaies guineensis) and microalgae, effect of thiamine towards the immune system of
plants, potential of seaweeds as a source of bioactive compounds, the hunt for riboswitches in plants. She is supervising 3 ongoing Master of Science students. She has
been hosted by various awards.
zettynorhana@upm.edu.my
zettybyusof@gmail.com
Notes:

July 25-27, 2016

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