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double determination with an oxydation of the sample with H202.

TOTAL SO2
Method: colorimetric, λ = 420 nm Linearity
Code product: SHPE036042 For a initial sample volume of 0,050 ml and a final volume after reaction
of 2,050 ml, method is linear until 300 mg/l of total SO2.
Constitution: 125 ml + 125 ml (125 tests)
Conservation: 4-8°C Precision
For in-vitro diagnostic only In a double determination, using 0.050 ml of the same sample and a final
volume after reaction of 2.050 ml, we can obtain a difference of
Principle (Réf.1) absorbance (Abs) which moving from 0.010 to 0.015 units, which
At pH 8, free and combined SO2,, react in proportional quantity correspond to a concentration variation in total SO2 of 1.5 – 2.0 mg/l.
with dissulfide introduced in excess. A coloured compound is
produced, which could be measured at 420 nm. Sample preparation: Generaly not predictable.
Increasing of optical density at 420 nm is proportional with
quantity of total SO2 present in the sample.
Bibliographie
Réf.1 - Determinazione di gruppi solfidrilici in materiale biologico
Composition du kit – JF Murray, Biochem. (1967)
1 x 250 ml CHROMOGEN - Sulphydril modifying reagent – JE Bodwell et al.
content: DTNB Biochemestry (1984)
Ethanol
K2HPO4, H3PO4
Note
1. A proportional variation of the reaction volumes does not change the
Stabilisants
results.
1 x 250 ml BUFFER
2. We suggest do not mix Reagents from different Production lots.
content: K2HPO4, H3PO4
5. PAY ATTENTION!
Stabilisants
Applications on routine Analyzers may be totally different from
Preparation and stability of solutions what we developed as manual determination, and also from
1. CHROMOGEN: ready to use. Solution is stable until the themselves.
final lifetime of the kit. 6. The reagent must be used only for the intended destinations, by
2. BUFFER: ready to use. Solution is stable until the final expert people and in the due lab. conditions.
lifetime of the kit.
Significations of printed pictogrammes
Nb:
During conservation, buffer could content turbidity, due to Reagents for in-vitro diagnostics only
sursaturation of one of compents. The presence of the solid
modify nothing in the reacting quality of reagents. Lot number

Méthode
Wavelenght: 420 nm See leaflet
tube: minimum 3 ml
Temperature: ambiante Provider
Final volume: 2.050 ml
Measure against distilled water.
Distributor
White White sample
reagent Standard Sample
Distilled H2O 0,050 ml Lifetime
Standard 160 mg/l 0,050 ml
Sample 0,050 ml 0,050 ml
Chromogen 2,000 ml 2,000 ml 2,000 ml Limit values low and high for keeping temperature
Buffer. 2,000 ml of the kit

Mix and wait 5 minutes at ambiant temperature. Measure


absorbance (Abs) of final solution.

Calculate:
(Abs sample - Abs white reagent) - Abs white sample.
-------------------------------------------- x 160 = mg/l total SO2
(Abs standard - Abs white reagent)

Specificity
This method is specific of sulfate groupes.
There is no interferences with ascorbic acid. But to avoid
interference due to anthocyanes and polyphenols, we can make a

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