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TOTAL SO2
Method: colorimetric, λ = 420 nm Linearity
Code product: SHPE036042 For a initial sample volume of 0,050 ml and a final volume after reaction
of 2,050 ml, method is linear until 300 mg/l of total SO2.
Constitution: 125 ml + 125 ml (125 tests)
Conservation: 4-8°C Precision
For in-vitro diagnostic only In a double determination, using 0.050 ml of the same sample and a final
volume after reaction of 2.050 ml, we can obtain a difference of
Principle (Réf.1) absorbance (Abs) which moving from 0.010 to 0.015 units, which
At pH 8, free and combined SO2,, react in proportional quantity correspond to a concentration variation in total SO2 of 1.5 – 2.0 mg/l.
with dissulfide introduced in excess. A coloured compound is
produced, which could be measured at 420 nm. Sample preparation: Generaly not predictable.
Increasing of optical density at 420 nm is proportional with
quantity of total SO2 present in the sample.
Bibliographie
Réf.1 - Determinazione di gruppi solfidrilici in materiale biologico
Composition du kit – JF Murray, Biochem. (1967)
1 x 250 ml CHROMOGEN - Sulphydril modifying reagent – JE Bodwell et al.
content: DTNB Biochemestry (1984)
Ethanol
K2HPO4, H3PO4
Note
1. A proportional variation of the reaction volumes does not change the
Stabilisants
results.
1 x 250 ml BUFFER
2. We suggest do not mix Reagents from different Production lots.
content: K2HPO4, H3PO4
5. PAY ATTENTION!
Stabilisants
Applications on routine Analyzers may be totally different from
Preparation and stability of solutions what we developed as manual determination, and also from
1. CHROMOGEN: ready to use. Solution is stable until the themselves.
final lifetime of the kit. 6. The reagent must be used only for the intended destinations, by
2. BUFFER: ready to use. Solution is stable until the final expert people and in the due lab. conditions.
lifetime of the kit.
Significations of printed pictogrammes
Nb:
During conservation, buffer could content turbidity, due to Reagents for in-vitro diagnostics only
sursaturation of one of compents. The presence of the solid
modify nothing in the reacting quality of reagents. Lot number
Méthode
Wavelenght: 420 nm See leaflet
tube: minimum 3 ml
Temperature: ambiante Provider
Final volume: 2.050 ml
Measure against distilled water.
Distributor
White White sample
reagent Standard Sample
Distilled H2O 0,050 ml Lifetime
Standard 160 mg/l 0,050 ml
Sample 0,050 ml 0,050 ml
Chromogen 2,000 ml 2,000 ml 2,000 ml Limit values low and high for keeping temperature
Buffer. 2,000 ml of the kit
Calculate:
(Abs sample - Abs white reagent) - Abs white sample.
-------------------------------------------- x 160 = mg/l total SO2
(Abs standard - Abs white reagent)
Specificity
This method is specific of sulfate groupes.
There is no interferences with ascorbic acid. But to avoid
interference due to anthocyanes and polyphenols, we can make a