Professional Documents
Culture Documents
Service Manual
English
Service Manual Centrifuge 5804/5804 R and 5810/5810 R, English 5804 920.104-12
Safety precautions 1
Disassembly / Assembly 4
Alignment / Adjustment 5
Diagrams 6
Technical data 8
(blank) Maintenance 9
Descriptions 10
Service Manual Centrifuge, English 5804 920.104-12
Centrifuge 5804/5804 R and 5810/5810 R
Contents
1 Safety precautions
Warning and hazard symbols in this Service Manual ..................................................... 1-1
Risk to persons ................................................................................................................... 1-2
Risk of damage to device................................................................................................... 1- 2
Risks through incorrect handling of centrifuge .............................................................. 1- 3
Risks through incorrect handling of rotors...................................................................... 1- 3
Risks through extreme stress on test tubes.................................................................... 1- 4
Risks from products, chemicals and extreme ambient conditions ............................... 1- 4
Emergency cut-off.............................................................................................................. 1- 5
4 Disassembly / Assembly
5804/5804 R and 5810/5810 R Lid .................................................................................... 4-1
Disassembling the lid ...................................................................................................... 4-1
5 Alignment / Adjustment
Adjusting magnet to Imbalance PCB ............................................................................... 5-1
Mounting procedure for adjusting the sensor idle value of Imbalance PCB .................. 5-1
Adjusting microswitches for lid latch ............................................................................... 5-1
Adjusting motor for lid latch with crank and torsion spring........................................... 5-2
Motor unit for lid latch compl. following assembly ......................................................... 5-3
6 Diagrams
58XX Steuerung (control).................................................................................5810 810.809
58XX Steuerung (control).................................................................................5810 810.906
Übersichtsplan 58XX Electronics....................................................................5810 901.006
8 Technical data
Technical data..................................................................................................................... 8-1
Certificate for the specifications of .................................................................................. 8-2
Eppendorf Centrifuge 5804 ............................................................................................. 8-2
Eppendorf Centrifuge 5804 R.......................................................................................... 8-3
Eppendorf Centrifuge 5810 ............................................................................................. 8-4
Eppendorf Centrifuge 5810 R.......................................................................................... 8-5
9 Maintenance (blank)
10 Descriptions
Hardware description of the centrifuge 5810/5810 R ..................................................... 10-1
General design................................................................................................................. 10-1
Specifications for the serial interface of the Centrifuges 5804/5804R/5810/5810R .... 10-10
Control via the serial interface ......................................................................................... 10-10
Pin assignment and connection to a PC ......................................................................... 10-10
Data transmission protocol ............................................................................................. 10-11
Adjust request (Select Telegram)..................................................................................... 10-12
Permitted format of VAL .................................................................................................. 10-12
Telegram format .............................................................................................................. 10-13
Examples of telegram ...................................................................................................... 10-13
Interface parameters of Centrifuges 58xx ....................................................................... 10-14
Control codes .................................................................................................................. 10-14
Control and status words ................................................................................................ 10-15
Setting the serial address of the centrifuge..................................................................... 10-16
Rotor table ....................................................................................................................... 10-16
Notes for centrifuge operation......................................................................................... 10-17
Symbol Meaning
DANGER
Risk of electric shock with potential for severe injury or death as a consequence.
DANGER
Risk of explosion with potential for severe injury or death as a consequence.
WARNING
Warning of potential injury.
CAUTION
Refers to slight risk or risk of material damage.
Risk to persons
Risk of explosion!
→ Do not operate the device in rooms where work is being carried out with explosive
substances.
→ Do not use this device to process explosive or highly-reactive substances.
Warning! Personal injury and damage to property through objects within safety
zone
Objects within the safety zone of the device will be set in motion by the vibrations and
may cause accidents.
→ Maintain a safety clearance of 30 cm between the device and other objects
according to the recommendations of EN 61010-2-020.
Risk! Harm to health from contact with toxic liquids and pathogenic
microorganisms
→ When handling toxic liquids or pathogenic microorganisms belonging to risk
group II (see World Health Organization: Laboratory Biosafety Manual), make sure
you comply with the relevant national regulations.
→ When working with pathogenic microorganisms belonging to a higher risk group,
more than one aerosol-tight bioseal must be provided for. ?(Bioseals are a
component of biosafety systems which are however not capable by themselves of
ensuring the protection of people and the environment when pathogenic
microorganisms are being handled.)
3. Have careful professional cleaning of the device carried out. The instructions
about cleaning and disinfection included in the operating manual should be
observed here.
Emergency cut-off
We recommend that you include this information together with chapter 2 of your service
manual for Centrifuges 58XX,
in order that all relevant product information can be found in one place.
The first Centrifuges 5804 / 5804 R / 5810 / 5810 R have already been successfully on the market
since 1996.
The hardware electronics have changed rapidly since then, and despite continuous adjustment,
some components are now no longer available.
The stoppage of delivery of replacement parts and support will take place in December 2009.
The rotors can still be used for the Refresher Centrifuges (5804/5804 R/5810 as of serial number
10.000 and for the 5810 R as of serial no. 12.000) when the correct care measures have been
applied in the past and there are no scratches or cracks!
Please note that the following rotors have a maximum period of usage of 7 years:
A-2-DWP ; A-2-MTP ; A-4-44 ; A-4-62 ; A-4-81 ; T-60-11 ; F-34-6-38
Be sure to document the commissioning date if the rotors are to be further used.
Replacment parts for which the delivery guarantee is ending in December 2009
Redesigned nameplate for centrifuges with the new 12-digit serial number (example):
Please note that, depending on the type of instrument, it can take some time until the first
devices with the new nameplate and the new serial number reach the market.
Wir empfehlen, diese Info Ihrem Service Manual Centrifugen 58XX, Kapitel 2, beizufügen,
damit alle relevanten Produktinformationen zusammenhängend abgelegt sind.
---------------------------------------------------------------------------------------------------------------------------
We recommend that you include that information together with chapter 2 of your service manual for
Centrifuges 58XX, in order that all relevant product information can be found in one place.
For logistics reasons the spring lifter had to be assigned new order numbers, please use
the current spare parts list at our homepage www.eppendorf-support.com
Old order number: 5804 001.109 New order number: 5814 850.058
Old order number: 5810 002.001 New order number: 5820 850.078
Error 1 Error in measurement system for – Check the rotational system on the
rotational speed. rotor.
– After repair:
False connection of cable U2/V2/W2
from motor to converter.
Device brakes, even when the lid is open. – Carry out a continuity check of the
microswitches while they are closed.
No actions possible, after a reset the port – Replace the "Control" PCB.
lines are constantly reset to 1.
Error 12 EDP error Check the interface PCB and the plug
connections for correct positioning.
– Serial PC interface: error during trans-
mission.
Error 13 EDP error Check the interface PCB and the plug
connections for correct positioning.
Serial PC interface: reception error, parity
error, wrong code number, check sum
error.
Error 14 EPROM check sum error after mains on. – Replace the EPROM or the
PROCESSOR (IC1).
– Error program memory, EPROM
defective. – Replace the "Control" PCB.
– Service program 6,
check temperature sensors,
condenser and bottom of rotor
chamber.
Device switches off – braked stop, the – Check all plug connections X3, X5,
refrigeration aggregate is switched off, X10.
the fan keeps running. – Check whether the refrigeration
Let the refrigeration aggregate cool aggregate is filled.
down, check the air intake.
Check whether the condenser fan rotates
when the aggregate is switched on.
– During run and stand-by. The fan is – Remove interference in the environ-
controlled with full power. ment. Test the centrifuge in another
room with a different device circuit.
– After mains on: The device is not rec-
ognized as a refrigerated device!!
Error 31 – x 0: Timeout while closing the lid. – Check the connections on the sensor
board and plug X10.
1: Timeout while opening the lid.
Display Cause
Error 32 Hardware error: imbalance measuring – Check the connections on the sensor
system. board and plug X10.
Error 33 Measuring values outside of the – Check the connections on the sensor
measuring range, too little space between board and plug X10.
sensor and magnet.
Error 35 Imbalance sensor idle value exceeds the – Check the connections on the sensor
limit (670 ± 100). board and plug X10.
– see Error 34
Display Cause
Display Cause
200 rpm New rotor was used in devices with older soft- Install new software and carry out
ware imbalance adjustment.
Display Cause
Display Cause
Standby Centrifuge not used for 6 hours Open lid and close again.
off (from software 2.0 onwards).
Display Cause
Display Cause
CCI – An old FC with software 2.1 was used. Use new FC with extended temperature
false range.
Problems with defective frequency converter, motor, brake resistors, overload, Error 6
6. Service Manual, "Service function, Program 13, Viewing the error table".
2 : x x y y y z z z z z
10. With Error 6: Service Manual, Customer service program 9, current error indication
(selectable via cursor key only directly after error)
9 : E r x x y y z z
Check the non-refrigerated centrifuges 5804/5810 to see whether the foam parts in the device are still
suitable to maintain the required ventilation flow for cooling.
By actuating simultaneously the PLUS and MINUS buttons (cursor keys) the device is switched over to
the service indication mode. This does not depend on the operational mode the device is currently in.
The run of the centrifuge is not influenced by this.
During a run only the indications no. 1...6 are accessible (from START to opening the lid or resetting to
the nominal value indication with START).
During a fault the indication automatically jumps to 9. After that you can start again switching to different
indications (error indication with display of the error location and for error 6 of the FC error numbers).
The FC parameters can be checked during a standstill and while the lid is closed (FC on!) with the
indication 2 and by actuating the MINUS button.
1: Temperatures
1 :
1 : x x * y y • y z z • z
2 : x x y y y z z z z z
xx – Motor current 33 =^ 3.3 A (phase current in the phase line to the motor)
y – F = FC on, display for fixed-angle rotor (only for factory setting)
yyy – parameter 9, display for swing-bucket rotor (only for factory setting)
zzzzz – parameter 3 rotational field - rotational speed/control value (only for factory setting)
2 : P x x V = y y y y y
3 : x x x * y y y z z z
4: Rotor recognition
4 : x x x y z z
xxx – Rotor relation (rotor table, rotor relations, see last page of Section 3)
y – number of recognition measurements
zz – Rotor number (rotor table, rotor relation, see last page of Section 3)
5: Imbalance monitoring
5 : x x x r u = z z z
5: Imbalance monitoring
5 : x x x y y y z z z
6: Lid locking
6 : u u u x1 x2 y z z z
7 : w w x x y y z z
ww – last error
xx – error report before last etc.
8: Version
8 : x x x y y z z z z
9: Current error indication (selectable via cursor key only directly after error)
9 : E r x x y y z z
xx – error number
yy – no. for locating the cause (for factory settings only)
zz – FC error number, if the frequency converter switches off with error 6
0 =no error message
1 =undervoltage
2 =overvoltage
4 =overtemperature FC
5 =overtemperature motor
7 =overcurrent FC
8 =interference EEROM FC
10 =no release
FA =reset by internal software and hardware
FF =serial interface timeout
General description
The service programs run independently from the normal operating modes.
You enter the program selection by simultaneously pressing the PLUS-/MINUS key and switching the
centrifuge on.
Display:
S E x x
The required program number can be set by actuating the PLUS or MINUS key.
If "error 36" is displayed immediately after calling up the service programs the EEPROM must be
initialized (see section Initializing).
The service mode can only be quit by switching off the device.
Program overview
5 Initializing
7 Cycle test
8 Rotor recognition
15 Display of the switching off values for imbalance / sensor idle value / power
supply
16
Deleting of the operating hours / error table
17
(factory only)
18
(factory only)
1 • x x x x x
xxxxx = RS232
BBUS Selection by means of the PLUS button
disable
2. n x x
Factory setting: 20 H
By actuating the PLUS button the address to be set is continuously counted upwards.
Acknowledgement of the selected device address with START.
END – end of program – actuate the START button.
START Program 2
S P x x x
START Program 3
START Program 4
y y y l r s s x x x
Keys:
OPEN – lid open/closed – normal function
PLUS – pulse by pulse forwards
MINUS – pulse by pulse backwards
SPEED – erase counter for continuous lid latch test.
STOP – end of program
Initializing
START Program 5
I n i t 2
The nominal values set and the operating conditions are stored in the EEPROM after each run of the
centrifuge. The limits for switching off (imbalance) and statistical data and fixed programs are also
stored in the EEPROM.
When calling up the program via the serial interface – code no. 64, additionally the interface is set to
20 H/disable.
Switching the aggregates / temperature sensor test (only for refrigerated devices)
START Program 6
K A L M F U L K S = x x
Key:
TEMP – refrigerating aggregate on/off
PROG – motor fan on/off
SPEED – frequency converter on/off (no control of FC, only relay on PCB audible)
TIME – Condenser fan on/off
xx – fan step (0...16)
the step can be changed by means of the PLUS and MINUS buttons
T e m p = x x • x T K = y y
1 : x x * y y • y z z • z
Is the refrigerating system tight? Does the valve switch? Repair if necessary and refill cooling agent
Quantity – cooling agent R134A
5804 R 400 g
5810 R 470 g
Cycle program
START Program 7
This program is used for a continuous test (e.g. factory test) of the centrifuge. Always 3 runs with a
subsequent interval of 1 min are carried out. After the 4th run the interval is 10 min. The FC is switched
off after each run.
The nominal values of the run can be changed after the start of the service program (pre-setting:
nominal rotational speed of the rotor, 10 min, 4 °C).
4 0 4 0 0 0 1 0
P A U S E x x
An individual run can be aborted by means of the STOP button. The lid can be opened during the
interval.
You can only quit the cycle program by switching off the device.
START Program 8
r o t a t e
_ _ _ _ _ _ _ _ _ _ _
r v = x x x y z z
When the upper part of the housing has been taken off, the check must be done without the
rotor!
START Program 9
F U b x x x y y y
b – converter ready, from switching on the FC to READY indication approx. 1 s will pass
xxx – FC on/off
yyy – rotational field speed, if FC is switched on
START Program 10
The centrifuge determines the imbalance cut-off value of the individual rotors from all rotors in a rotor
group.
One angled rotor (W) and one swing-bucket rotor (A) must be used from each rotor group. As a
rule, this should be carried out with the standard rotors.
Exception: Use (A) Rotor A-4-81 if avaliable.
Standard rotors: Centrifuge 5804 / 5804R – (W) F34-6-38 and (A) Rotor A-4-44
Standard rotors: Centrifuge 5810 / 5810R – (W) F34-6-38 and (A) Rotor A-4-62
It is possible to weigh the weights yourself using Eppendorf tubes. Filled 2.0 ml tubes (approx. 3.2 g) are
recommended. The weight of the tube must also be taken into consideration.
With swing-bucket rotors, these should be placed flat, loose and without an adapter on the bottom of
the swing-bucket. With the angled rotor F34-6-38, water should be pipetted directly into the empty
bores. MTP rotors can be loaded and then filled with one MTP each.
2. Call up the service program: Hold down both cursor keys and switching on the device.
Display : SE 1
6. Three calibration runs are performed automatically. The lid opens automatically.
8. Close the lid and wait until three further calibration runs have been performed.
Lid opens automatically.
9. Insert weight G3. Close lid. Start run: Start (Centrifuge must show "Imbal" and switch off.)
11. Remove fixed-angle rotor (W) and insert swing-bucket rotor (A).
12. Using the corresponding imbalance amounts G1, G2, G3, carry out the procedure described in
points 2. to 9
13. Switch off the device. Remove the imbalance weights and then switch on the centrifuge again as
normal.
Note:
An imbalance adjustment must be carried out every time the motor, PCB Control or PCB Imbalance is
replaced or when the rotor is inserted!
After the motor has been replacedl or when the centrifuge has not been used for a long period, it should
run for approximately five minutes at maximum speed in order to ensure
that the vibration mounts have the normal degree of hardness.
Centrifuges: 5804
Rotor designation G1 G2 G3
A A-4-44 16g 22g 25g
A A-2-DWP 16g 22g 25g
A A-2-MTP 16g 22g 25g
Centrifuges: 5804 R
Rotor designation G1 G2 G3
A A-4-44 12g 18g 25g
A A-2-DWP 12g 18g 25g
A A-2-MTP 12g 18g 25g
START Program 11
3 0 0 2 0 0 0 1 1
START run
Display of the acceleration time in seconds (at 97 % of the rotational speed nominal value)
H L x x • x
B L x x • x
START Program 12
– The software sets a rotational speed above the switching-off limit. If the device operates correctly, it
switches itself off when reaching the switching-off rotational speed and stops without braking.
If the device is not switched off, the run must be stopped immediately by hand!
On switching on again the centrifuge brakes and is then again in the normal operational mode.
Overspeed cut-off:
The overspeed cut-off is about 300 1/min above the nominal speed of the rotor in use.
START Program 13
E r r o r x x y y y
xx – error no.
START Program 14
E A x x x x
C L x x x x
The load cycles are recorded separately for each rotor type.
START Program 14
E A x x x x
C L x x x x x x
C L y y x x x x x x
yy – rotor number CL
h x x x x x
START program 15
U y y x x x
yy – rotor number
xxx – switching-off values for all rotors (no. 2, 3, 4, 5, 8, 13, 14, 17, 18)
always continue switching with START
START
U r x x x
xxx – idle value imbalance sensor (measurement during the imbalance calibration)
Start
U 3 x x x
xxx – max. imbalance value for 30g-run G3 with rotor A-4-81 (from software 3.4 onwards)
Start
R W x x
Start
R A x x
Start
D V x x
Start
S P x x x
Start
X X y y
XX – P0, P1, PL
test words (check of the test process)
START program 16
E r a s e E r r o r
PROG – error table and the operating hours are being erased
acknowledgement – display ok
Rotor num-
max. speed/rcf
ber Rotor designation Rotor relation
max. radius
CL
1
3700 / 2250 1 2 153.9
2 147 mm A-2-DWP * *
143...163
4000 / 2253 192.3
3 A-2-MTP
126 mm 182...202
4000 / 3220 A-4-62 230.7
4
180 mm (rectangular bucket) 220...240
4000 / 2750 A-4-62 230.7
4
154 mm (MTP carrier) 220...240
3
4000 / 3220 A-4-81 * 269.2
5
180 mm (rectangular buckets) 255...283
3
4000 / 2915 A-4-81 * 269.2
5 163 mm (MTP carrier) 255...283
3
4000 / 2900 A-4-81 * 269.2
5
153 mm (Flex bucket) 255...283
6
7
5000 / 4500 384.6
8 A-4-44
161 mm 375...395
9
10
11
12
12000 / 15294 4 577
13 95 mm F-45-48-PCR *
563...591
5
12000 * / 18514 5 615.4
14 F-34-6-38 *
115 mm 605...625
15
16
14000 / 20817 F-45-30-11 730.7
17
95 mm FA-45-30-11 721...741
1
* Usable from software version 3.0 onwards
2
* From software version 3.3 onwards allowed up to 3700 rpm as well for older rotors labeled 3600 rpm
3
* Can be used from software version 3.5 onwards
4
* Usable from software version 3.7 onwards
5
* Centrifuge 5804 / 5804 R / 5810 only 11000 rpm
– Remove lid.
– Start device.
– 5804/5804 R only!
Remove 6 Phillips screws from rear panel.
– 5804/5804 R only!
Remove rear panel.
– 5804/5804 R only!
Disconnect plug from fan.
– Remove 2 Phillips screws at the rear side of the upper part of housing.
– 5404R/5810 R only!
Remove 2 Phillips screws at the right front side (cover of cooling aggregate).
– Take out black clamps of holder for front cover. This eases removal of upper part of housing.
– Pull side panels of upper part of housing outwards slightly and pull upwards.
– Start device.
– Disconnect 5 plugs.
Pre-condition: Remove upper part of housing, front cover and PCB Control.
– Unsolder cable.
– Start device.
– Replace motor.
Imbalance adjustment: Magnet must be aligned horizontally and vertically to Hall sensor.
Horizontal distance between magnet and
Hall sensor = approx. 9-13 mm, depending on magnet design.
– Remove cover.
– Disconnect plug X10 (PCB Imbalance detection/ Hall sensor /rotor temperature).
– Replace motor.
Imbalance adjustment: Magnet must be aligned horizontally and vertically to Hall sensor.
Horizontal distance between magnet and
Hall sensor = approx. 9-13 mm, depending on magnet design.
Pre-condition: Remove lid, front cover, upper part of housing and cover plate of PCB Control.
– Replace motor.
Imbalance adjustment: Magnet must be aligned horizontally and vertically to Hall sensor.
Horizontal distance between magnet and
Hall sensor = approx. 9-13 mm, depending on magnet design.
– Remove 4 Phillips screws from cover plate of PCB Frequency converter. Put cover plate aside.
– Fasten cover plate for PCB Control temporarily with 2 screws on housing.
– Disconnect plug X10 (PCB Imbalance detection/ Hall sensor /rotor temperature).
– Replace motor.
Imbalance adjustment: Magnet must be aligned horizontally and vertically to Hall sensor.
Horizontal distance between magnet and
Hall sensor = approx. 9-13 mm, depending on magnet design.
– Start device.
Clean the plane for mounting the unit and apply thin coat of heat sink compound on com-
plete surface using a spatula.
(Not required for new FC with extended temperature range.)
– Start device.
Clean the plane for mounting the unit and apply thin coat of heat sink compound on com-
plete surface using a spatula.
(Not required for new FC with extended temperature range.)
– Start device.
– Remove 3 Phillips screws from holding plate for motor of lid latch.
– Remove Seeger-ring.
– Start device.
– Pull plug.
– Remove 2 nuts.
– Replace fan.
– Start device.
– Start device.
Pre-condition: Remove lid, front cover and upper part of housing and motor of centrifuge.
– 5804 R only!
Lift PCB Control, pull plug X4 (fan).
– Replace fan.
– Start device.
Repair kit motor cap cpl. with rotational speed sensor (no. 5820 850.329)
containing:
Motor cap with rotational speed sensor
3 spacer bushings
3 screws
3 O-rings
O-ring 40 x 1 (for refrigerated centrifuge)
Insulating flange
Ferrite bead
Fastening material
For mounting the repair kit motor cap, push the 3 small o-rings onto the spacer bushings.
Note:
Always when mounting the motor cap, be sure that the rubber sleeeve does not slide onto the insulating
flange. This would result in a too high position of the motor cap on one side, thus causing the swing-
buckets of rotor A-4-81to strike there.
Retrofitting rotors
Retrofitting without installation is possible from the following serial numbers onwards:
Rotor Software Centrifuges / Serial numbers
5804 5804 R 5810 5810 R 5804 5804 R 5810 5810 R
230 V 230 V 230 V 230 V 120 V 120 V 120 V 120 V
T-60-11 3.0 1380 1796 1100 2925 1410 1820 990 2949
A-2-DWP up to 3,600 1/
3.0 1380 1796 1100 2925 1410 1820 990 2949
min
A-2-DWP up to 3,700 1/
3.3 1980 2421 1680 4305 1970 2493 1690 4461
min
A-4-81 / MTP / Flex 3.5 / / 2162 6191 / / 2186 6203
F-45-48-PCR 3.7 2904 3897 2306 6743 2904 3861 2366 6743
5810R:
230 Volt > 660 and < 1220 Frequency converter with extended 5810 855.250
temperature range required
120 Volt > 660 and < 1180 Frequency converter with extended 5810 855.241
temperature range required
230 Volt < 2162 NEW frequency converter required 5810 855.250
120 Volt < 579 Frequency converter with extended tempera- 5810 855.241
ture range required
Only: 5804 / 5810 and 5804 / 5810 from serial no. 10,000
Modified mounting of PCB Imbalance recognition for Centrifuges 5804 and 5810:
Modify the delivered PCB Imbalance recognition No. 5820 850.027 if necessary.
Older centrifuges cannot be modified due to bore dimensions of the base plat.
drehen
turn
90°
5820 850.027
Please pay attention to the change to cable colors and partly to allocation for
older Centrifuges 5804 / 5804 R / 5810 / 5810 R (see drawing)!
grün - gn – green - GN
weiß - ws– white - WH
blau - bl – blue - BU
grau - gr – gray - GY
weiß - ws – white - WH
Horizontal distance – Magnet to Hall sensor approx. 9–13 mm depending on magnet carrier Imbalance
recognition.
ca. 9–10 mm
approx. 9–10 mm
ca. 12–13 mm
approx. 12–13 mm
Mounting instructions
Aids: Hand drill
Drill, ø 3.6 mm, with 10 mm drilling-depth limit.
Mounting instructions
Removing rotor
Turn rotor nut anticlockwise using the rotor key, remove rotor in an upward direction. If this is impossible,
tighten the rotor nut back up, close the centrifuge lid and set the temperature in the rotor chamber to the
removal temperature in the range from 10 °C to 30 °C. Turn rotor nut anticlockwise using the rotor key,
remove rotor in an upward direction.
If this is unsuccessful: undo the rotor nut and use both hands to pull the rotor vigorously upwards using
a gentle rocking and tilting motion. It may be helpful for a second person to hit the rotor key in the rotor
gently with a plastic hammer at the same time to loosen the jam on the motor shaft.
Please follow the section in the operating manual entitled "Fitting and removing the rotors".
Magnet must align horizontally and vertically to Hall sensor of Imbalance PCB!
Horizontal distance magnet to sensor approx. 9 to 13 mm.
– Switch on centrifuge (perhaps Error 20, because temperature sensor is not connected).
To avoid damaging the compressor, do not operate the centrifuge with cooling in normal
position within 4 hours. Disconnect the compressor plug on Control PCB for the test time if
required!
Both microswitches are actuated by the locking plates of the reinforcement for the lid mat and issue the
start signal for the lid latch motor. Set the switches so that they switch simultaneously when the lid is
closed. The switches should not respond too early, when the lid is just lying loosely on top, otherwise the
latch might not reach its end position and be switched immediately to Lid Open.
Set/check using Service program SE 4.
3 11
1 2 4 97 8 6 5 10
Fig. 1
Adjusting motor for lid latch with crank and torsion spring
Secure the screws/nuts no. 6, 9 and 11 with Loctite 243 (medium strength).
Note: actuate motor externally at approx.10 - 24 VDC or using Service program SE 4 to put the drive
shaft in a different position.
A) Fit motor for lid latch (1) on motor mounting angle (2).
B) Push crank compl. (3) onto drive shaft (4). Crank for the lid latch (5) remains mobile.
C) Secure crank compl. (3) on drive shaft (4) using screw (6) (see Figure 2 following assembly).
D) Turn adjustment ring (7) 30°– 45° to the right (clockwise) which puts torsion spring (8) under
initial tension. Now secure adjustment ring (7) using screw (9).
E) Perform a function check: put the rotor key in crank for the lid latch (5) and check that it will
turn through approx. 170° anticlockwise (and against the tension of the torsion spring).
F) Put the motor in a favorable installation position of crank for the lid latch (5) to facilitate assembly
in the centrifuge. Fit motor unit (Figure 2) and connect the cable.
G) Once the motor unit has been installed or replaced, select a different installation position for crank
for the lid latch (5), put on the slide for the lid latch and and install the washer (10) and tighten with the
nut M5 (11). If there is still a crank with peg, tighten the circlip.
Fig. 2
2 X9 8
Warning : X12 IC10
For continued protection against R2
2 26 1 R46
LM393
fire hazard replace only with the C44 R55
G same type and rating of fuses. 1 25
R60
R20 R45
R61
R27
R26
R29
R25
R49 R21
Fuse 120/202V 230V R48 R43
C23
F4 R34 R33 C22
F3 T300mA T125mA
R13
R11
GL3 ULN2003
R5
IC8 C17
1
14
C40
IC3
R30
TRAFO R31 TMS27PC512
C8 KK2
74HCT573
F5 IC2
C12 SPR2
C51
C11 R9
C10
GL2 C13
R50
R51
F C24
C16
7
C18
8
R6
IC1 R10
R36
RES
80C517A
C30
xxx
TLE4260
74HC04
R19 R47
C19
IC6
C29
KK1 SPR1 SEN1
C45 C46
F3 WS SW C28
3
4
D7
D9
D8
D10
WS X2 SW D6
DR3
TRI1
IC4
X7 X7_2 Q2
2
14 11 12
1
C5 24 21 22
K4 FM24C04
R12
1
C32
C33
1 X5 IC9 L298N
E VR4 C50
N PE U1
14 11 12 1
C6 C47
24 21 22 K3 C34
VR3
14 11 A
12 K6 B X61
1
ULN2003
X4
IC7
D1 OK1 ROTF
2
R3 R42 X17
MCT275
R59
C21
R4 R39
1
74HCT14
K5 R37
C3 C4 R38
IC5 R32
C25 X18
X3 X11
1 2
DR2 R7
C31 GND
D
4
R17
X6
1
VR2 R16
VR1 VR5
C2
C27
R18 C38
10
R23
3
DR1 R22
X8
VERTRAULICH
CONFIDENTIAL
C37
2
R35
R14
R62
X10
1
2
C1
D2
1
C35
R1
10 1
C20
R44 R8
1
C X1 D3 C26
R15 X15
C39
EP.LP. 946-7
A-Nr : 97/
A
DV_Nr: 0015_512.946-7
Auftr_Nr. : 000-00000
58XX_Steuerung
Masstab: 1:1
eppendorf Gepr.:
Datum: 31.01.99/roth
5810_810.809.04
11 10 9 8 7 6 5 4 3 2 1
only cooled F4 RXE110
24V 24V 24V
58xx
Cooling- Frequency SPR2 24V 24V
24V 24V VCC
24V 24V
Version 1
24V
1 2
24V
24V
Compressor converter F3 TRAFO KK2 24V
1 2 2 7824 VCC VCC
X3 X2 24V
2
7 14 1 3 24V
X3
X3
max. 200mA
2
$$$ GL3 FBP005 IN OUT
X2
X2
X2
2
2
1 2 230V(120)
2
VR5 U
1
100N
2
100N
2
100U
2
4700U
470N
2
2
GND
1
2
10K
R55
Warning:
3
2
1
R21
R5
K5 12 1 4
24K3
X1
1
1
1
6 2
5K11
VR2
1
1
R2
11 14
$$$
10K
C8
C44
C10
C11
For continued protection 20V/0.6A 8
1
R45
C40
2K21
U
1
SL
6
L_FU
N_FU
H SL against fire hazard -
2
1
L_KU
X1 2 3 8
1
replace only with the 2 7
2 1 - IC10
12 OC.
C39 100N 10n/230VAC C4 same type and rating 1 5
1
1 2 F5 500mA VCC VCC VCC1 IC10 + LM393
2 1 of fuses. GND2 2 GND2
GND 4,2 V OC.
10n/230VAC C3 10 1 2 100K 3 LM393
4 1 2 1 SP1 2 + 4
1K
R6
R46
L
C5 10n/250V
FUSE 120/202V 230V GL2 B250C1_5
2
X1 3 2 1 ca.260mA 4
1 2 1 2 K4 12
2
L L 50-60Hz 50-60Hz
2
2
2
11 14 1 TLE4260 5
2
IN OUT
2200U
470N
2
3 1 GND GND
470N
2
150N
2
1000U
100N
2
R20
2 1
5K11
F3 T300mA/UL T160mA/IEC 9.2V/0.4A SPR1DRES 4
1M
R1
R2610XB40 R2610XB40 C6 10n/250V 100N C24 SP4
1
1
$$$
2 1 3
1
1
1
1
1
VR1 U
2 1 RES 100K
C1
C2
C13
DR1 DR2
C12
K3 12 2 GND 1 2
C16
C17
R43
1
1
3 4 3 4 11 14 N_VE 1 8 3 1
2 GND
N N
N
TRF17 KK1
X1 1 GND2 2 GND1 1
G Line
RESET
2
VCC VCC
Version OK1 6
2
37=GND,14=VCC 4 SYN
2
4 MA_2
4U7
IC5 1
D7
K
D8
K
A K 1 5 1 X7_2
10U
100N
2
LIDR 1 2 DMA 5
R59
D1 74HCT14 IN1 VS
100K
EM513 IC6
7=GND,14=VCC 7 2
1
IN2 OUT1
1
1
74HC04
A
A
DM 10
C28
IN3 MA_1
C45
C46
IC9
D6
$$$$ 10K 1 12 3 1 X7
L_VE 1 2 2 4 1 2 IN4 OUT2
ES1B
ES1B
only cooled R3 MCT275 R4 LID 3 4 6
IC6 EN_A
7=GND,14=VCC ENB 11 13
2CN1206K40 1
Version gesickt aufsetzen GND GND EN_B OUT3 LID-Motor
GND 74HC04
VCC VCC
1 14
SEN1 OUT4
X11 ENAB
1K 15
97=GND,14=VCC 8 2 1 SEN2
Quarzgehaeuse gegen IC5 R32 24V
C32 22P Standard ist der L298N
74HCT14 (8=GND2,9=VCC) 24V
D9
K
D10
K
X11 1 GND verloeten 1 2 8=GND,9=+V 24V 24V VCC
12MHZ Quartzeinsatz
X11 2 1 IC8 16 24V X61
F VCC
C33 22P 12MHz C47 100N
A
A
VCC K4
2 1 1 2 RES ULN2003 8=GND2,9=24V 1 2 24V
1 3 4 X61
2
GND IN OUT
ES1B
ES1B
Compressor- Q2 GND 2=GND 7 IC7 10 A B GND2 2
60 40 3 X61
100N
2
temp. HWPD XTAL1 optional not cooled Version
ULN2003 GND2 2 GND2 2
2 X61
R7
10K
RESET 10 K3
1
RESET 8=GND2,9=24V 1 X61
C25
1K
1
ALE 50 39 1 EIN 117=GND,14=VCC 10 2 1 5 12 A B
1
C27
ca. 2.5V ALE XTAL2 OC VCC IC5 R37 IC7 Magnetcoil
11 IC2
GND1 C 8=GND2,9=24V ULN2003
10K
R50
bei 25’C GND1 1 GND1 1 PSEN 49 74HCT14 K6 12
2
PSEN 24V
52 DAT0 DAT0 2 19 ADR0 1 IC7 16 11 14
P0.0 1D 1Q
1
100N
69 53 DAT1 DAT1 3 18 ADR1 24V X4
OWE P0.1 2D 2Q VCC VCC ULN2003 only cooled
100N
2
VCC1 54 DAT2 DAT2 4 17 ADR2 8=GND2,9=24V
R11
P0.2 3D 3Q 24V C34 L_ML
274R
82 55 5 16 1K
10n/250V 1 X4
RO P0.3 DAT3 DAT3 4D 4Q ADR3 MOTL 57=GND,14=VCC 6 2 1 6 11 K6 Version 1 2
1
56 DAT4 DAT4 6 15 ADR4 R38 IC7
2
P0.4 5D 5Q IC5
VR3
C21
LID-key- VCC1 11 57 DAT5 DAT5 7 14 ADR5 74HCT14 ULN2003 N_VE
$$$
VAREF P0.5 6D 6Q B A 2 X4
58 8 13 8=GND2,9=24V 8=GND2,9=24V
U
position P0.6 DAT6 DAT6 7D 7Q ADR6
2
100N
2
12 59 DAT7 DAT7 9 12 ADR7
2
1 VAGND P0.7 8D 8Q 4 13 3 14 Motorfan
10K
R12
VCC
74HCT573
GND GND IC7 IC7
1
10K 4 36 ENAB GND ULN2003 ULN2003
3 2 1 PE-SWD P1.0 10=GND,20=VCC
10K
R51
C29
E SEN1OUT R10
P1.1
35 SUMM
K5
51 34 KL 8=GND2,9=24V X5
1
2 E/A P1.2
1
GND 33 ROTF SDA 1K
1
P1.3 KUHL 137=GND,14=VCC 12 2 1 2 15 A B N_VE
100N
2
UGN3503 TZ2 78
P8.0 P1.4
32 ROTF SCL IC5 R39 IC7 480uH/0,8A
1 X5
VR4
GND GND GND1 1 TZ1 79 31 8=GND,9=+V ULN2003 3 1 1 4 2 1
74HCT14
$$$
P8.1 P1.5
1
80 30 1 6 TRI1 0,1/250 C50 L_KL
U
SDA
R36
P8.2 P1.6 NC SCL 2 15 DR3 2 X5
562K
C18
81 29 SCL IC8
2
P8.3 IC1 P1.7
2 7 ULN2003
2
A1 WP 1 Coolingfan
KEST 20 41 ADR8 100R 100R 4 2
GND1 1 GND1 1
P7.0 P2.0 IC4 KL 5 1 2 1 2
TKO 19 42 ADR9 3 5 IC6 6 R47 R19 S202T01
KPOS
P7.1 P2.1 A2 SDA 7=GND,14=VCC GND
18 43 ADR10 74HC04
P7.2 P2.2 FM24C04 GND
IMB 17 44 ADR11 X6
P7.3 P2.3 GND GND GND
16 45 ADR12 4=GND,8=VCC 274R
P7.4 P2.4 RMK 1 2 -RMK
1
2
24V 24V 15 46 ADR13 R16 1 X6
P7.5 P2.5 GND
14 47 ADR14
C30
1
24V P7.6 P2.6 2 X6
13 48 ADR15 EPROM 64K X 8 274R
LMK -LMK
R9
P7.7 P2.7 1 2
R62
R17 3 X6
274R
5K11
ADR0 11
2
A0 GND
70 21 RXD0 ADR1 10
2
P6.0 P3.0 A1 IC3 VCC 4 X6
2
1
100N
RXD2* 71 22 TXD0 ADR2 9
P6.1 P3.1 A2
D TXD2* 72 23 MOTL ADR3 8 16=GND,32=VCC
GND1 1 GND
P6.2 P3.2 A3 C20 100N GND LID-
1K
73 24 SYN ADR4 7 13DAT0 GND
R15
P6.3 P3.3 A4 DQ1 1 2 position
2
FREE 74 25 LMK ADR5 6 14DAT1
GND P6.4 P3.4 A5 DQ2 VCC VCC
READY 75 26 RMK ADR6 5 15DAT2 X15
1
SELECT
P6.5 P3.5 A6 DQ3 C19 100N
76 27 ADR7 4 18DAT3
X10 P6.6 P3.6 A7 DQ4 1 2
10K
R14
77 28 ADR8 29 19DAT4 X8
P6.7 P3.7 A8 DQ5 GND
X10 4 VCC1 ADR9 28 A9 DQ6
20DAT5 V_BUS
1
VCI DB0 68 1 EIN ADR10 24 21DAT6 1 X15 LP"IFACE" X15 10 3 X8
X10 1 274R P5.0 P4.0 A10 DQ7
IMBS 2 1 DB1 67 2 KUHL ADR11 27 22DAT7
1
C31
X10 2 R35 P5.1 P4.1 A11 DQ8 SELECT ( OPTION ) I/O_BUS
GND1 DB2 66 3 LIDR ADR12 3 6 X15 X15 8 5 X8
X10 3 P5.2 P4.2 A12
DB3 65 5 LID ADR13 30
2
P5.3 P4.3 A13 RXD0 GND_BUS
2
Imbal DB4 64 6 LIDAN ADR14 31 3 X15 X15 9 7 X8
K
D3
K
D2
P5.4 P4.4 A14
10U
100N
DB5 63 7 EN ADR15 2
37=GND,83=GND,38=VCC,84=VCC
P5.5 P4.5 A15 TXD0 GND
DB6 62 8 RW 23 4 X15 X15 5 8 X8
R44
P5.6 P4.6 E
1
DB7 61 9 RS
VERTRAULICH
CONFIDENTIAL
A
A
P5.7 P4.7
C35
80C517A 25 2 X15 X15 7 1 X8
2 562K 1
G TMS27PC512
5810_840.007
GND
ZMM8_2
ZMM4_7
1 1 1 1 1 1 VCC GND GND
6 X8
PSEN
R13
274R
GND1 GND1 GND1 GND1 GND1 GND1 TXO
2 X8
2
C VCC VCC RXO
C23 100N 4 X8
GND 1 2
VCC C22 100N
X10 5
KEST 1 2 VCC VCC VCC VCC VCC
X10 6 VCC VCC Interface
GND
24V
2
Rotor-
1
temp. 24V
100N
2
ca. 2.5V
1
1
1
R8
10K
bei 25’C not mounted
1
R33
2K21
C26
1
10K
R48
10K
R30
10K
R31
2 0R 1 2 0R 1
2
GND1 1 GND1 1
R60 R61
2
2
2
VCC 1
9 8
VCC Start- Code- IC6 3,36V
100R 7=GND,14=VCC 8=GND,9=+V 8=GND,9=+V X9
1 2 impulse impulse 74HC04
R22 274R 0R
RXD2* 1 2 14 IC8 3 10 IC8 7 2 1 TXD
1 R49 R25 8 X9
10K
68N
2
2 1 2 1 SUMM 11 ULN2003 ULN2003
R18 IC6 10 L298N 1,16V V_ON
VCR 10U C37 7=GND,14=VCC 8=GND,9=+V 7 X9
X10 7
1
B ROTF 17=GND,14=VCC 2 ROTF 74HC04 274R
X10 8 R23 FREE 4 13 1 2 RXD+
C51
X10 9
GND 2 1K 1 IC5 IC8 R26 1 X9
74HCT14 1 2 SP3 1
LIDAN 13 12 ULN2003 FREI+
C38 3N3 IC6 8=GND,9=+V 4 X9
Rotation 7=GND,14=VCC GND
4,4/0,7V
1 2 74HC04 TXD2*
274R RXD-
GND GND
5 IC8 12 1 2
1 15 R27 2 X9
ULN2003 2 0R 1 OK_OUT
8=GND,9=+V R29 5 X9
VCC1 VCC VCC 274R
3,5V
Pinning READY 1 2 11 IC8 6 1 SP2 2 FREI-
AUSFUEHRUNG LP-SCHILD IC R34 3 X9
VERSION PCB-LABEL DR1 DR2 TRAFO R3 VR1..VR5 R50 R51 ULN2003
GND Supply
ROTF
5804_811.003 74HCT14D 7 14 Converter-
GND control
2
120V/50-60HZ 5805_811.007 R3008X60 BV13067 18k2 S14K150 GND
VCC1
VCC1
TZ1
TZ2
DB7
GND
DB6
GND
DB5
GND
DB4
GND
DB3
GND
DB2
GND
DB1
GND
DB0
VCC
RS
VCC
RW
EN
LIDA
SUM
GND 74HCT04D 7 14
5810_811.007
5811_811.000 X12
9
8
2
7
1
6
5
4
3
R42
5K11
ULN2003AD 8 9
26
25
24
23
22
21
20
19
18
17
16
15
14
13
12
11
10
GND
5804_810.007
1
5805_810.000 SAB80C517A 37,83 38,84
X12
X12
X12
X12
X12
X12
X12
X12
X12
X12
X12
X12
X12
X12
X12
X12
X12
X12
X12
X12
X12
X12
X12
X12
X12
X12
A 230V/50-60HZ R2610XB40 BV13068 39k2 S14K300 For service
5810_810.000 FMC24C04D 4 8
GND
5811_810.004 Keyboard/Display 58xx Steuerung
5804_812.000 OUTX L298N 8 9
purposes only
5805_812.003 Blatt 1 von 1
1
1
202V/50-60HZ R2610XB40 BV13070 39k2 S14K300
5810_812.003 eppendorf
X17
X18
5811_812.007 Gepr.:
Zentrifugen Datum: 01.01.99
5810 810.906-04
11 10 9 8 7 6 5 4 3 2 1
58xx
POS. TYP Connector and LP"Interface"
X3 AMP MATE-N-LOCK 2-pol. are optional Chassis
X1 AMP MATE-N-LOCK 3-pol.
LID-position serial Interface
X2 WAGO-Klemme 3-pol. 1 2 5 ( RS 232 and BBUS complete)
H X4, X5 WAGO-Klemme 2-pol. LID-
X6, X61 AMP MODU-II 4-pol. left right 6
motor Mikroswitch
X7 FSH 4.8
X7_2 FSH 6.3
X8, X9 AMP MMT-Buchsenl. 8-pol. Masseband
X15 AMP MMT-Stiftleiste 10-pol.
X10 WAGO-Stiftleiste 10-pol.
X12 AMP Einloet-STV 26-pol.
X11 AMP MODU-II 2-pol.
KEYBOARD/
G
DISPLAY
VCC
1
X8 X17 X18 2 VCC
X6
1
2
3
4
5
6
7
8
SUM
FUSES Version 230V Version 202V X7 X7_2 1 2 3 4 3
LIDA
GND
RX0
GND
4
ROTF
TX0
not not EN
V_BUS
5
cooled cooled cooled cooled RW
6
5
7
8 I/O_BUS
9 GND_BUS
RS
F1 T 10A T 8A MT 12A T 10A 7
X15 8 DB0
X15
X15 10
X15
X15
X15
F2 T 10A T 8A MT 12A T 10A GND
9
F DB1
LP - 58XX_Steuerung 10
LP IFACE GND
11
DB2
12
GND
Main plug Main switch 13
DB3
Version FUSE F3 14
GND
15
X15
X15
X15
X15
X15
ws DB4
16
6
4
3
2
1
1 GND
2 120V-50/60Hz T300mA/UL 17
sw DB5
3 18
GND
19
X1 DB6
20
19
230V-50/60Hz T160mA/IEC 21
19 GND
with Fuses F1/F2 with Thermoswitch DB7
(only 120V) 22
19
E (only 230V,202V) TZ2
23
19
202V-50/60Hz T300mA/UL TZ1
24
19
VCC1
25
19
VCC1
X12 26
X12
1 1
2 2
see also connection shematic X61 3
X5 siehe auch Anschlussplan 4
Zei.-Nr. 5810_901.014-
ws (white) 1
D sw (black) 2 1 2 1 2 3 1 2 3 4 5 6 7 8 1 2 3 4 5 6 7 8 9 10 1
X4 2
X3 X2 X9 X10
X11
weiss(white)
schwarz(black)
gnge(grye)
VERTRAULICH
CONFIDENTIAL
bn(brown)
bl(blue)
C
Suppression Motor- Magnet-
Compressor Filter 1 2 3 4 Rotor- Fan coil
temp. imbal temper.
not cooled
sensor
cooled Version Version
N Magnet
U1
PE 1 2 3 4 5 6 7 8 9 10
Temperature
Switch
Overtemp.-switch
A Only for cooled Version ! For service
purposes only Uebersichtsplan 58xx Electronic
only 5810 Brake-
120V-Version resistor Blatt 1 von 1
eppendorf Gepr.:
Zentrifugen Datum: 01.07.99
5810 901.006-03
Centrifuge 5804/5804 R and 5810/5810 R
7 Spare parts list
7–2 7–5
7–4
7–3
7–9 7–9
10–3
7–7
11–1
11
Fig. 1
5–3 1–2
Fig. 2
Fig. 3
Fig. 4 12–5
1–3 Fig. 5
1–6
Fig. 6
10–9
Fig. 7
Fig. 8
1–9
Fig. 9
Abb. 10
7–1
Abb. 11
7–1
Abb. 12
7–5
Abb. 13
7–5
Abb. 14
5804 R
5810 R
Fig. Pos. Designation Part no.
5804
5810
1 Drive
5 1-3 Motor cap complete with rotational speed sensor x x x x 5820 850.329
2 Brake resistors
3 Fan
3-2 Fan for motor 230 V / 202 V (base plate) x x 5821 850.071
4 Accessories
5804 R
5810 R
Fig. Pos. Designation Part no.
5804
5810
4-2 Fuses 8 A slow blow x x 5810 352.002
5 Electronics
5804 R
5810 R
Fig. Pos. Designation Part no.
5804
5810
3 5-2 EPROM software program for 5810/5810 R x x 5810 810.108
3 5-2 EPROM software program for 5810/5810 R for SMD x x 5810 850.100
3 5-2 EPROM software program for 5804/5804 R for SMD x x 5804 850.106
5-6 Retrofit kit EDP connection, rear panel, 5804 x 5814 850.031
5-6 Retrofit kit EDP connection, rear panel, 5810 x 5820 850.264
5-8 PCB Interface 58xx (with EDP connection cable) x x x x 5811 855.504
6 Temperature sensors
6-1 Temperature switch for fan of power unit, 120 V x 5804 623.002
7 Lid
11 7-1 Upper part of lid 5810/5810 R /with monitor glass*2 x x 5810 160.510
12 7-1 Upper part of lid 5804/5804 R with monitor glass*2 x x 5804 160.517
5804 R
5810 R
Fig. Pos. Designation Part no.
5804
5810
1 7-3 Phillips screw for lid, 50 long x x 0110 000.995
14 7-5 Lower part of lid 5810, with rim and bore* x 5810 160.529
13 7-5 Lower part of lid 5804, with rim and bore* x 5804 160.525
7-5 Lower part of lid 5804 R, with rim and monitor glass* x 5805 160.529
7-5 Lower part of lid 5810 R, with rim and monitor glass* x 5811 160.522
8 Mains input
3 8-1 Mains switch with thermal fuse (120 V vers.), 15 A x x 0113 203.218
3 8-1 Mains switch with thermal fuse (120 V vers.), 12 A x x 0113 203.080
8-3 Fuse holder for mains input module (230 V) x x x x 0113 200.189
8-3 Fuse holder for mains input module (202 V) x x x x 5820 850.434
5804 R
5810 R
Fig. Pos. Designation Part no.
5804
5810
8-6 Retrofit PCB Interference mains filter, 120 V, 5810 x 5810 855.020
8-7 Retrofit PCB Interference mains filter, 120 V, 5810 R x 5810 855.039
9 Control pad
1 9-4 Plastic cap for emergency release, front cover x x x x 0113 001.607
10 Lid latch
5804 R
5810 R
Fig. Pos. Designation Part no.
5804
5810
11 Housing (without mounting parts)
11-6 Rear panel, lamella protection, 5804 R (retrofit kit) x 5815 850.027
11-6 Rear panel, lamella protection, 5810 R (retrofit kit) x 5821 850.268
1 11-8 Guiding rail for shell for defrosted water x x 5811 010.032
12 Refrigerating system
5804 R
5810 R
Fig. Pos. Designation Part no.
5804
5810
4 12-5 Starter capacitor, 230 V x x 5810 855.144
*1 Installation into the 5804 R is only possible with 4 new bores in the base plate,
as well as 4 new bores in the base plate in order to relocate the compressor.
*2 Note: the enclosed starter capacitor and starter relay must be used.
5804 R
5810 R
Pos.
Fig.
5804
5810
Fixed-angle rotor with lid, F-34-6-38 x x x x 5804 727.002
Lid for F-34-6-38 x x x x 5804 727.509
5804 R
5810 R
Pos.
Fig.
5804
5810
Deepwell-plate rotor carrier (2 pcs.) for A-2-DWP x x x x 5804 743.008
Rubber base plate (2 pcs.) x x x x 5427 702.008
Name:
Tel.:
Order no.:
5804 R
5810 R
Qty. Fig. Pos. Designation Part no.
5804
5810
1 Drive
2 Brake resistors
3 Fan
5 Electronics
6 Temperature sensors
5804 R
5810 R
Qty. Fig. Pos. Designation Part no.
5804
5810
7 Lid
9 Control pad
10 Lid latch
Name:
Tel.:
Order no.:
5804 R
5810 R
Qty. Fig. Pos. Designation Part no.
5804
5810
1 Drive
2 Brake resistors
3 Fan
5 Electronics
6 Temperature sensors
5804 R
5810 R
Qty. Fig. Pos. Designation Part no.
5804
5810
4 6-5 Temperature sensor (condenser) x x 5821 850.098
7 Lid
8 Mains input
3 8-1 Mains switch with thermal fuse (120-V vers.), 15 A x x 0113 203.218
3 8-1 Mains switch with thermal fuse (120-V vers.), 12 A x x 0113 203.080
9 Control pad
10 Lid latch
Power supply 230 V 120 V 230 V 120 V 230 V 120 V 230 V 120 V
Max. power requirement 750 W 850 W 1,350 W 1,300 W 900 W 950 W 1,350 W 1,300 W
8A 10 A 8A 10 A
Fuse protection
time lag time lag time lag time lag
Overcurrent protection 12 A 15 A 12 A 15 A
Weight 55 kg 80 kg 68 kg 99 kg
Rotor: A-4-44
Tube: 4 x approx. 50 ml
Measurement Procedure:
Rotational speed measurement: Load the rotor with the specified tubes (tubes should be filled with
ethylene glycol).
Allow the centrifuge to accelerate to the specified rotational speed.
After five minutes, check the rotational speed.
Measuring instrument used: Stroboscope (accuracy: ≤ 1 %).
Time measurement: Set the run time to continuous operation. Wait for five seconds. Start
the centrifuge by pressing the Start key.
Measure the time which elapses until the display switches from 4 min
to 5 min.
Measuring instrument used: Stopwatch (accuracy: < = 0.1 s)
We herewith certify that each centrifuge is tested according to the above procedures and meets the given
specifications.
Hamburg, 15.05.2001
Eppendorf AGr
Rotor: A-4-44
Tube: 4 x approx. 50 ml
Measurement Procedure:
Rotational speed measurement: Load the rotor with the specified tubes (tubes should be filled with
ethylene glycol).
Allow the centrifuge to accelerate to the specified rotational speed.
After five minutes, check the rotational speed.
Measuring instrument used: Stroboscope (accuracy: ≤ 1 %).
Time measurement: Set the run time to continuous operation. Wait for five seconds. Start
the centrifuge by pressing the Start key.
Measure the time which elapses until the display switches from 4 min
to 5 min
Measuring instrument used: Stopwatch (accuracy: < = 0.1 s)
Temperature measurement
(Refrigerated centrifuges): Load the rotor with the specified tubes (tubes should be filled with
ethylene glycol).
Pre-cool the rotor to 6 °C using the Fast Cool function.
Centrifuge for 45 minutes at the specified rotational speed. Brake the
centrifuge using the maximum braking level.
Immediately after the centrifuge has come to a standstill, stir the
contents of the test tubes and measure the sample temperature in the
middle of the bucket (room temperature: 23 °C ± 2 °C)
Measuring instrument used: Temperature measure with thermo-
sensor, 0.5 mm diameter (accuracy: < = 0.5 °C).
We herewith certify that each centrifuge is tested according to the above procedures and meets the given
specifications.
Hamburg, 15.05.2001
Eppendorf AG
Rotor: A-4-62
Tube: 4 x approx. 50 ml
Measurement Procedure:
Rotational speed measurement: Load the rotor with the specified tubes (tubes should be filled with
ethylene glycol).
Allow the centrifuge to accelerate to the specified rotational speed.
After five minutes, check the rotational speed.
Measuring instrument used: Stroboscope (accuracy: ≤ 1 %).
Time measurement: Set the run time to continuous operation. Wait for five seconds. Start
the centrifuge by pressing the Start key.
Measure the time which elapses until the display switches from 4 min
to 5 min.
Measuring instrument used: Stopwatch (accuracy: < = 0.1 s)
We herewith certify that each centrifuge is tested according to the above procedures and meets the given
specifications.
Hamburg, 15.05.2001
Eppendorf AG
Rotor: A-4-62
Tube: 4 x approx. 50 ml
Measurement Procedure:
Rotational speed measurement: Load the rotor with the specified tubes (tubes should be filled with
ethylene glycol).
Allow the centrifuge to accelerate to the specified rotational speed.
After five minutes, check the rotational speed.
Measuring instrument used: Stroboscope (accuracy: ≤ 1 %).
Time measurement: Set the run time to continuous operation. Wait for five seconds.
Start the centrifuge by pressing the Start key.
Measure the time which elapses until the display switches from 4 min
to 5 min.
Measuring instrument used: Stopwatch (accuracy: < = 0.1 s)
Temperature measurement
(Refrigerated centrifuges): Load the rotor with the specified tubes (tubes should be filled with
ethylene glycol).
Pre-cool the rotor to 6 °C using the Fast Cool function.
Centrifuge for 45 minutes at the specified rotational speed.
Brake the centrifuge using the maximum braking level.
Immediately after the centrifuge has come to a standstill, stir the
contents of the test tubes and measure the sample temperature in the
middle of the bucket (room temperature: 23 °C ± 2 °C)
Measuring instrument used: Temperature measure with thermo-
sensor, 0.5 mm diameter (accuracy: < = 0.5 °C).
We herewith certify that each centrifuge is tested according to the above procedures and meets the given
specifications.
Hamburg, 15.05.2001
Eppendorf AG
General design
The centrifuges of the series 58XX consist of the following main electrical assembly groups:
– Assembly group "Control" with input filter, power supply unit, central control unit, frequency
converter interface, drive and position recognition of the lid locking mechanism, connections for
imbalance monitoring and rotational speed monitoring. For the refrigerated version there are also
the temperature monitoring and the elements to control the condenser fan, refrigeration aggregate
and motor fan.
– Assembly group "Display/Operation" consisting of the keypad (snap washer under the foil),
LCD display module incl. driver and background illumination and signal transmitter.
– Assembly group imbalance monitoring consisting of a PCB with an analogue Hall sensor and
OpAmp. for signal processing.
– Frequency converter CCI 1000 of the Hanning company to operate a 3-phase asynchronous motor.
– As an option we offer a PC interface, that can be retrofitted in the form of a plug-in module and an
interface cable.
– Various cables.
Power supply
Power is supplied via a plug module that is dimensioned according to the voltage version in use:
The main switch is installed externally in the housing. The PCB "58xx Control" is connected via
plug "X1".
The PCB is installed in the front side of the device and screwed to the top and bottom plate.
– imbalance monitoring
– converter interface
– power supply for the refrigeration aggregate, the motor fan and the converter
– temperature monitoring
The power supply unit consists of the components transformer, rectifier bridges GL_2, GL_3, voltage
regulator SPR1 or SPR2 and filter capacitors.
• non-stabilized DC voltage as a driver voltage for the locking mechanism of the lid
Apart from that SPR1 supplies a reset signal if the voltage drops below 4.8 V.
The processor works with a cycle frequency of 12 MHz which is generated by an external oscillator. This
oscillator is designed as an oscillatory circuit consisting of a12 MHz quartz and two 22 pF capacitors.
The operating system of the centrifuge is stored on the EPROM.
With the EEPROM it is possible to store current operating parameters such as the rotational speed, the
duration of the last run, the operating hours etc. These data remain in the EEPROM even when the
centrifuge is switched off, they can be called up when the centrifuge is switched back on.
Apart from that the selectable programs are stored in the EEPROM.
These functions are carried out with the help of a unipolar Hall sensor with an open-collector output. A
"pull-up" resistor (R32) in the output of the sensor is used as a load resistor which supplies a defined
signal at the processor input if e.g. a cable break occurs between the sensor and the PCB “58xx
Control“. The Hall sensor (HAL506UA) is soldered to a sensor board which is located underneath the
motor covering in the rotor chamber.
Under the influence of the magnetic field of the coded magnet bar in the lower part of the rotor the
output of the sensor becomes conductive against ground. The sensor board is connected to the control
PCB via the plug connector X10. The pulses are supplied via a triggered inverter (IC5) at PORT 1.3 and
1.4 of the processor.
During the rotation the magnetic strips on the rotor move past the Hall sensor and according to the
length of the strips they generate “LOW“ pulses which are analyzed as rotational speed pulses. The
magnetic systems of the rotors consist of a strip with even length (starting magnet) and a strip with
variable length which enables coding of the rotors. By making the relation, a large number of rotors can
be coded. The rotational speed has no influence in this context. R23 and C38 serve to reduce interfering
pulses (low-pass filter) and IC 5 serves to improve the pulse steepness.
For measuring purposes the rotational speed pulses can be taken from OUTX(X17).
Imbalance monitoring
The imbalance monitoring is done by a system which analyzes the distance between a magnet fastened
to the motor and a Hall sensor which is installed in a fixed position on the motor plate. This sensor is an
analogue Hall sensor (UGN3503U) in which a voltage is induced that corresponds to the strength and
polarity of the magnetic field. This voltage is applied to the A/D converter in the processor (PORT 7.3).
Depending on the position of the motor the magnetic field of the magnet influences the sensor with
different field strengths which in turn changes the induced voltage at the output.
The voltage is proportional to the deflection. This enables an analysis of the imbalance that can be
exactly reproduced. An RC combination (R14, C31) serves as a low-pass filter that dampens interfering
pulses.
The sensor is integrated on the PCB "Imbalance detection" which amplifies the Hall signal. This
assembly group is installed at a distance of approx. 10 mm from the initial magnet.
In order to achieve a sufficient signal the operating voltage of the OpAmp. on the imbalance PCB is 8 V.
The signal is generated on the control PCB from the 24V supply by means of a Z stabilization.
Since the analogue voltage at the processor input to be analyzed may not exceed 5 V, the voltage is
limited by a 4.7 V Z-diode (D2).
In order to measure the rotor chamber temperature, an NTC resistor is used which is encapsulated in a
sleeve and located on the rotor chamber base.
Depending on the temperature the sensor (NTC 10 k / 25 °C) changes its resistance. Via a voltage
divider (R8) this leads to a change of the voltage at the analogue input (PORT 7.0) of the processor.
Condenser temperature
In order to measure the condenser temperature, an NTC resistor is used which is fastened at the output
of the compressor.
This sensor (NTC 10 k / 25°C) serves as a protection against overtemperature. Via X11 and a voltage
divider (R7) it is connected to the analogue input of the processor PORT 7.1.
The locking mechanism consists of the lid motor drive, the lid position request and the position request
of the locking mechanism.
The necessary current is supplied to the lid motor by means of an IC driver (IC9). Depending on the
signal at the ports 4.2 and 4.3 the motor can rotate in left-hand or right-hand direction. Normally the
motor will rotate in left-hand direction when it opens as well as when it closes the lid. In case of a fault
(e.g. the locking bar is blocked) the motor will be rotated in right-hand direction after a certain time
(approx. 2 s) to loosen the blocking. For a short period of time (less than 1 s) during the locking process
it can draw up to 1.5 A current. This reduces the transformer voltage from 30 V to approx. 15 V.
The “ENABLE“ line, controlled by PORT 4.5, blocks the outputs of IC 9 when the locking mechanism for
the lid is inactive.
Two microswitches are used for the position request of the lid. They are located at the slots in the
housing in which the brackets of the lid are inserted. When these brackets are inserted into the slots
PORT 3.4 and 3.5 are connected to GND. In this way it is recognized that the lid is closed and can now
be locked.
In order to recognize the position of the locking mechanism the junction plate has one magnet each on
the upper and lower edge with opposite magnetic polarity. An analogue Hall sensor (SEN1) is located on
the control PCB which is influenced by these magnets. Without the influence of a field the voltage at the
sensor is approx. 2.5 V. When the lid is locked the lower magnet is positioned above the sensor and the
voltage is approx. 2.7 V. If the lid is unlocked the upper magnet is positioned above the sensor and the
voltage is approx. 2.3 V. Since this voltage can be measured at ANALOGPORT 7.3 it is possible to
request the position.
A condenser is installed in the refrigeration circuit which is used to distribute the heat from the
compressor to the environment. Additionally the condenser is supplied with cooling air by a fan.
The fan regulation is done phase-controlled. This is done by a solid state relay (TRI1) which influences
the voltage at the fan and is controlled by the processor. In this application there are only two steps; full
load during the run and fixed load in stand-by mode.
The necessary phase analysis (D1, R3, OK1, R4) of the mains voltage is done by means of the phase
analysis circuit. D1 suppresses the negative half wave and the positive half wave is switched by the
opto-coupler onto a trigger. This generates a rectangular signal, which is used by the processor to
control the rotational speed of the fan.
Power supply for the refrigerating aggregate, the motor fan (lifting magnet) and the converter
These 3 aggregates operate with the mains voltage. For the individual aggregates the power is supplied
in the following way:
– the lifting magnet (non-refrigerated version) directly from pin 15 and 16 of the IC7
All relay contacts are equipped with a radio shielding capacitor to minimize sparks when switching off.
On the refrigerating aggregate relay an RC circuit (R2, C7) is used for this purpose.
Converter interface
The communication between the control and the converter is done on the basis of a serial data
transmission (RS232). This makes it possible to change the converter parameters during a run to achieve
an optimal run of the centrifuge. During a run telegrams are constantly exchanged, in this way a
malfunction of the converter can be immediately analyzed.
The data input to the converter is done by port 6.2 and the data output from the converter is done by
port 6.1.
Additionally a release signal (PORT 6.4) is transferred to the converter which reports its readiness to
PORT 6.5.
PC interface
The centrifuge series 58XX can be equipped with a standard serial interface RS232C and with an
Eppendorf specific interface (BBUS).
For this it is necessary to retrofit the module "IFACE" on the control PCB and to retrofit the 9-pole cable
in the device with SUB_D connector (9-pole). Then it is possible to communicate directly with the
centrifuge via a PC.
X15 is the plug connection and X8 the plug for the SUB_D cable.
■ 1
TX n 2
RX n 3 RS232C
x n 4
GND n 5
n 6
V_BUS n 7
I/O_BUS n 8 BBUS
GND_BUS n 9
This assembly group contains all components that are needed to operate the centrifuge with a PC or an
Eppendorf-BBUS.
The BBUS operation is done by opto-couplers and is thus uncoupled from the RS232C. This interface is
operated by a BBUS converter which is externally connected or by the Eppendorf BUS system.
By means of the data selector IC (IC1) the signals TXD0 and RXD0 are either switched to the BBUS
interface or to the RS232C interface.
The frequency converter is used to convert the single-phase alternating voltage into a 3-phase
alternating voltage which powers the 3-phase asynchronous motor (no collector). The output of the
converter is approx. 1100 W. The converter is equipped with a basic data set which can be updated with
the required parameters via the interface.
This makes it possible to adjust optimal acceleration and brake curves, also the power consumption and
the power loss can be reduced to a minimum.
The converter contains the electronic braking circuit which uses the external braking resistors to reduce
the excess energy which is generated (the motor acts as a generator).
Keypad
The keypad request is done by a resistor matrix that contains tactile switches. By pressing a key a
certain voltage divider is generated. The voltage value is either higher than TZ1 or TZ2 at the analogue
inputs PORT 8.0, 8.1. To each key a certain voltage value is assigned.
Display
The display is an LCD module with a driver/decoder and background illumination. The intensity of the
illumination is fixed. The brightness of the display can be set at the potentiometer POT1.
The control of the display is done by 3 control lines and 8 data lines.
– control lines EN from PORT 4.5 enable signal for the driver
RW from PORT 4.6 read / write command
RS from PORT 4.7 selection whether the incoming byte (DB0...DB7)
is a control command or a data byte
The standstill of the device and the readiness to open the lid of the centrifuge is indicated by the "OPEN"
LED LED1 (PORT 4.2).
The buzzer SUM1 signals the completion of the centrifuge run or a fault during the centrifuging process
(PORT 1.1).
The centrifuge can be controlled together with other laboratory devices via the BBUS as well as
individually via a PC and an RS 232 interface. During operation via BBUS, several different devices can
be connected to one BBUS interface and controlled by a master PC.
The centrifuge functions as a "slave", meaning that it must be addressed via an external EDP. A test with
an interface tester is only possible when the centrifuge and the EDP are connected.
A voltage of approx. minus 9.5 volt should be measured at the EDP plug output (TX) of the centrifuge
when not connected to the EDP.
Ground= pin 5
In service program 1, set the interface type and the device address.
The Centrifuges 58xx have two different interfaces on a 9-pin SUB-D plug.
The pin assignment for the 9-pin male SUB-D plug is as follows::
RS232 2 = TX
3 = RX
5 = GND
BBUS 7 = V-BUS
8 = I/O-BUS
9 = GND-BUS
Telegram format
– 1 start bit
– 8 data bits
– 1 stop bit
Send request
STX CNR CNR CNR = VAL VAL VAL VAL VAL ETX BCC
If the incorrect code number is entered, the centrifuge responds with NAK.
An adjust request allows a nominal value or a control command from the PC to be transmitted to the
centrifuge.
EOT TYP ADR STX CNR CNR CNR = VAL VAL VAL VAL VAL ETX BCC
– Max. 20 characters are accepted (e.g. 3000.000). The figures after the decimal point are not
processed. The centrifuge requires a maximum of 5 figures. (VAL – type integer)
– Figures 0...9
– The value is set to the left, i.e. the highest position follows the equal sign
Examples of telegram
04 21 20 30 31 30 05
02 30 31 30 3D 34 30 30 30 03 0B
04 21 20 02 30 31 30 3D 34 30 30 30 03 0B
04 21 20 02 30 31 33 3D 2D 35 03 14
Control codes
10H = START
20h = STOP
40h = OPEN
2= Acceleration phase
4= Deceleration phase
6= Free deceleration
0= Lid open
1= Lid disengaged
3= Latch backwards
4= Latch forwards
6= Lid closed
The Service programs run completely separately from the normal operating procedure. By simultane-
ously pressing both register keys and by switching on the main power supply at the same time, the user
arrives at the program selection menu.
Display:
1 • x x x x x
Display:
2 • x x
When the PLUS key is pressed, the address which is to be set is incremented.
START – To confirm the address selected
After the address has been set, the centrifuge must be switched off and switched on again in order to
restore the normal operating status.
Rotor table
The rotor number should be set after the rotor has been exchanged to enable every nominal rotational
speed value to be specified:
With the exception of Short spin and the programming of fixed programs, all machine functions can be
operated via the serial interface. The fixed programs of the centrifuge cannot be used, although this
function may be simulated on a computer by transferring fixed data records.
The parameters 10, 11, 12, 13, 15, 16, 17 may be modified during a centrifugation run. Unlike operation
using the keypad, the run time (Par. 12) cannot be calculated using the time which has already expired.
The value which is transferred is valid as the new nominal value.
For technical reasons, it is not possible to switch during the braking phase from free deceleration
(Par. 17 =0) to a braking level (Par.17= 1...9).
Period of use
The service life of a rotor/bucket depends on different parameters like care, material and of course on
the factors type and scope of runs.
Eppendorf rotors and buckets have a max. period of use of 7 years from initial commissioning.
No fixed-angle rotors have any restriction on period of use with the exception of F-34-6-38 and T-60-11.
Please follow the instructions in the operating manual and the rotor supplement sheet with specific
reference to measures for looking after rotors.
Once period of use has expired, rotors and buckets should be decommissioned at once!
Visual check/cleanliness
Examine rotors/buckets for dirt residues and check that they have a smooth, clean surface. The
Eppendorf markings should be clearly legible. The bores of the fixed-angle rotors should be checked
with the torch for deposits and for residues of chemicals (use an endoscope if possible).
Aluminum rotors are protected against common laboratory chemicals by an anodized coating. No traces
of corrosion from chemical or mechanical effects, such as falls, blows, scratches, cracks and damage
which may be due to glass from broken tubes not having been removed may be present.
For Rotor A-4-62 with MTP carriers only: ensure that there are no traces of abrasion on the rotor caused
by careless insertion/removal of MTP carriers (not guided in straight).
Clean with a clean, grease-free, damp cleaning cloth and then dry.
Perform a visual inspection (using torch if necessary). Check for score marks which can be caused only
by the customer not securing the rotor properly with the rotor key.
Assess the thread of the motor shaft of the centrifuge (use torch if necessary). Check that the rotor can
be tightened up properly clockwise using the rotor key so as to test the rotor nut as well. After the rotor
nut has been turned through approx. two revolutions, the threads must mesh and it should no longer be
possible to take off the rotor. Turn the nut right in up to the stop and check that it is properly and firmly
located.
Swing-bucket rotors
Clean the pivots, they must be free of contamination. Is there any obvious wear? Clean the buckets and
suspend them in the rotor to check possible swing path. With the bucket inserted in the rotor, check
whether it is possible for the bucket to swing evenly at all positions without jerks when swinging
backwards and forwards. Grease lightly with Eppendorf pivot grease.
Rotor cross: have the distances between the individual arms of the rotor cross changed as a result of
incorrect use? With a bucket suspended in the rotor, check whether even swinging is possible at all
positions without jerks when swinging backwards and forwards.
Note: only use original Eppendorf pivot grease, order no. 5810 350.050, other types may go
resinous or sticky! It is recommended that a few runs be started in the centrifuge before
routine operation to distribute grease evenly.
Buckets
Clean grooves, which must be free of contamination. If there is visible wear on the bearing surface of the
pivots, this may be a result of aggressive cleaning in a dishwasher. Clean the buckets and suspend them
in the rotor to check possible swing path. With a bucket inserted in the rotor, check whether it is possible
for the bucket to swing evenly at all positions without jerks when swinging backwards and forwards.
Grease grooves lightly with Eppendorf pivot grease.
Note: only use original Eppendorf pivot grease, order no. 5810 350.050, other types may go
resinous or sticky! It is recommended that a few runs be started in the centrifuge before routine
operation to distribute the grease evenly.
Weight classes
Buckets are sorted by weight. The weight class is embossed into the side of the groove, see illustration:
e.g.. "_60" (the last 2 digits in grammes if the empty bucket weighs 260 g, for example).
Then place all buckets on a scale, consecutively if possible, and check for deviations.
In this example "60", the weight must be between 260 g and 260.9 g with empty and cleaned
rectangular buckets A-4-44.
Note: a mixed arrangement of 2 opposing MTP carriers with 2 rectangular buckets or 2 Flex
buckets is permitted only on swing-bucket rotor A-4-81
Use with only two buckets inserted is prohibited for all swing-bucket rotors!
Are the side walls bent as a result of too many plates or the wrong, non-approved plates having been
inserted? The cause may also be a plate inserted carelessly at the edge of the bucket.
Under no circumstances continue using buckets with bent side walls.
Watch out for deformation.
If the baseplate is damaged or cracked or the screws are oxidized as a result of aggressive chemicals or
inadequate cleaning, then replace them. In the process, replace plastic baseplates on all buckets with
rubber baseplates.
Information: to replace the rubber baseplate: undo screws, remove baseplate and clean bucket. Ensure
that the new rubber baseplate is located centrally. Do not use any adhesive such as Loctite or similar;
gently tighten new screws alternately to 0.6 Nm so that no unnecessary deformation is caused.
Fixed-angle rotors
Examine the bores of the fixed-angle rotor. Are there any cracks, changes inside or underneath the
rotor? Perform the examination using the torch (or endoscope, if appropriate). Any residues of chemicals
or the like will have to be removed for this purpose. Is there visible efflorescence/are there visible
material changes at the edge of the rotor under the seal?
This is often caused by the customer not taking adequate care of the rotor.
Rotor lid
Perform a visual inspection, in particular of the lid screw with thread (not for Rotor F-45-30-11). Check
that circlip and spacer are securely located.
Place rotor lid on a smooth surface and inspect it for an out-of-round, deformed appearance.
Locking mechanism
Information: the rotor lid and the rotor thread can be greased lightly with Eppendorf pivot grease, order
no. 5810 350.050 to minimize noise when tightening.
Rotor F 45-30-11
On this rotor, the lid is simply placed on top and is held on the rotor during operation by physical
conditions.
The O-rings should be replaced as a precaution during a service.
O-ring for lid mounting, order no. 0013 578.109
O-ring for rotor (set of 5 pcs.), order no. 5498 900.137
The aerosol-tight rotor lid has two sealing rings: one on the lid screw and another in the lid groove. The
aerosol-tight rotor lid may only be used with undamaged, clean seals. Frayed, worn or dirty seals do not
provide adequate protection and may lead to loss of aerosol-tightness. Damage may also occur as a
result of incorrect autoclaving operations.
The rotor lid should be replaced with a new aerosol-tight rotor lid every 10 autoclaving cycles (121 °C, 20
min.). Please check regularly that seals are undamaged! Rotor lids with defective seals may not continue
to be used and should be replaced by a new rotor lid. It is not permitted to replace the seals!
Information: the rotor with aerosol-tight rotor lid is tested using the Porton Down test. Tests are also
offered by other institutes. However, it is cheaper to replace the existing lid with a new aerosol-tight rotor
lid tested by the factory.
Please note: to prolong the life of the seal, do not store aerosol-tight rotors with the lid
screwed up tightly!
The old aerosol-tight rotor in silver has the aerosol-tight sealing ring in the rotor and not in the lid. The
sealing ring (set of 2 x 5) order no. 5498 900.013 continues to be available and replaceable.
However, a test of leaktightness cannot be performed.
Information: the aerosol-tight rotor with lid has passed the Porton Down test. Tests are also offered by
other institutes, though it is cheaper to replace the rotor complete with rotor lid with a new one if
required. The silver design is no longer available.
Check whether the locators for the adapters are still firmly assembled. Check for visible damage.
Rotor marking
Date of manufacture
Gas spring
The gas spring should be inspected by a service technician at least once a year. The gas spring should
replaced as a precaution every 2 years.