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Project Description

Identification of the three commonly used


1 medicinal plants by the T'bolis of South
Cotabato in treating diseases.

Fieldworks focusing on the usage of

2
medicinal plants. Data on this phase were
primarily obtained through series of
observation, interview, validation, and
documentation.
Assessment of the antimicrobial properties

3 of the medicinal plants, toxicity screening


and determination of secondary metabolites
through phytochemical screening.
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Research Objectives

Identification of the three commonly used


1 medicinal plants by T’bolis of South
Cotabato.

2
Variation of the medicinal plants in terms of
Family, parts used and process of preparation
in traditional medical treatment.

3
Determination of the antimicrobial activities
of the different medicinal plants.

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Research Objectives

Determination of significant difference in


4 the mean zone of inhibition of bacteria and
fungus.

5
Determination of secondary metabolites found
in the medicinal plants.

6
Determination of the toxicity levels of the
different medicinal plants.

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General Procedure

Prior to the conduct of the study

Courtesy call to Sending of


Barangay communication
Captains and to proper
T’boli leaders authorities

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Field Protocols

Validation of
Collection of
Series of answers of
Plant
interviews the
Samples
respondents

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Laboratory Protocols

Preparation of Antimicrobial
Treatments Assay

Brine Shrimp
Phytochemical
Assay for
Screening
Toxicity Level

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Photo documentation

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Findings

Medicinal Properties according to


T’bolis: For toothache, headache,
vomiting and diarrhea

Preparations:
Flowers are directly smelled and dabbed
to forehead and neck 3 times a day for 3
days for headache and vomiting.
Flowers and roots are chewed 3 times a
day with continuous use for toothache.

Superstition:
Bidens pilosa L. Small flower is placed on the child’s
navel for diarrhea.
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Findings

Medicinal Properties according to


T’bolis:
For pimples

Preparations:
Leaves and fruits are directly applied
three times a day with continuous
use for pimples.

Superstition:
When you eat tilapia eggs, your
pimples will return.

Ludwigia prostrata Roxb.


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Findings

Medicinal Properties according to


T’bolis:
For urinary tract infection (UTI) and hair fall

Preparations:
Roots are boiled and the decoction is drunk
immediately 3 times a day with continuous
use for urinary tract infection (UTI).
Roots are also used as shampoo every bath
for hair fall.

Superstition:
You should not let other people use your
comb or your hair fall will not be cured.
Eleusine indica
(L.) Gaertn. www.themegallery.com
Findings
Plant Extracts with
Species Antimicrobial Properties

B. pilosa L. (flowers)


S. aureus L. prostrata Roxb. (leaves)
E. indica (L.) Gaertn. (roots)

B. pilosa L. (flowers)


E. coli L. prostrata Roxb. (leaves)
E. indica (L.) Gaertn. (roots)

B. pilosa L. (flowers)


A. niger L. prostrata Roxb. (leaves)
E. indica (L.) Gaertn. (roots)
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Findings
Secondary metabolites
Plant Plant Parts present
 Alkaloid
B. pilosa L. Flowers  Tannin
 Steroid

 Alkaloid
B. pilosa L. Roots  Tannin
 Free fatty acid

 Tannin
 Free fatty acid
L. prostrata Roxb. Leaves  Flavonoid
 Steroid

 Free fatty acid


E. indica (L.) Gaertn. Roots  Steroid
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Table 5. Median Lethal Concentration (LC50) of the Plant
Extracts Using the Brine Shrimp Lethality Assay

Plant Extracts Common Names LC50 µg/mL

B. pilosa L. (flowers)
Keluon melel bulok 0.00008
B. pilosa L. (roots) Keluon melel bulok 39.33

L. prostrata Roxb. (leaves) Bulung henus 4.01

E. indica (L.) Gaertn. (roots) Keluon fet 5.11

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Conclusions

1. T’bolis use a variety of plants for treatment of common


diseases. B. pilosa L. and L. prostrata Roxb. are herbs
while the E. indica (L.) Gaertn. is a grass.
2. The three plants are classified into three families
namely Asteraceae (Compositae), Onagraceae and
Poaceae (Graminae).
3. Majority of the plants could inhibit the growth of the
two representative bacteria and one representative
fungus.
4. There is a significant difference in the zone of inhibition
of bacteria and fungus after 24 and 48 hours.
5. Plants tested have varying content of secondary
metabolites.
6. Plants tested have high toxicity levels.
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Recommendations

1. Conduct of cytotoxicity study using human


lymphocytes
2. Conduct of other equally important bioassays like
anti-inflammatory, antiangiogenic, and anti-viral
activities on the three identified plants
3. Determination of specific levels of secondary
metabolites.
4. Conduct of bioassay on other plants identified to
establish their antimicrobial activity, the active
secondary metabolites present and toxicity levels;
and
5. Conduct of other equally important bioassays like
anti-inflammatory, antiangiogenic, and anti-viral
activities on the three identified plants.
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