This Study Resource Was: Do Not Open The Exam Until Instructed!

BioMG1350 Prelim.
2- K Spring 2014 - ANSWER KEY
DO NOT OPEN THE EXAM UNTIL

INSTRUCTED!
The exam will start at 9:05 and end at 9:55
While you wait to start the exam, please fill out your name, ID and version of this
test on the Scantron form. Do NOT fill out your birthdate
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LAST NAME (space) FIRST NAME 7 DIGIT STUDENT ID NUMBER
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Make sure you fill in the information clearly; only use #2 pencil
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• TURN OFF CELL PHONES AND ALL ELECTRONIC DEVICES. Anyone
found touching an electronic device will be assumed to be cheating, and the
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test removed.
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• Put ALL belongings under your seat.

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• If you finish before 9:50, you may hand in your Scantron and leave.
AFTER 9:50 YOU MUST STAY IN YOUR SEAT UNTIL THE END OF THE
EXAM AND ALL EXAMS HAVE BEEN HANDED IN.
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• At the end of the exam, hand in just the Scantron form; you
may keep this set of questions.
• Suggestions: indicate your answers on the test and then
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transfer them to the Scantron.

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• Answer the easy questions first, and go back to the harder

ones.
• Mark only one response per question (the Scantron will
invalidate any answers with more than one entry)
• GOOD LUCK!
https://www.coursehero.com/file/12081236/Prelim-2-SP14/ The exam starts on the next page

BioMG1350 Prelim. 2-K Page 2
Q1. The version of this test is:

1. Version K ß THIS IS YOUR VERSION, PLEASE ENTER THIS ANSWER IN THE FORM!
2. Version L

Q2. Many proteins are completely synthesized in the cytosol and then targeted to the appropriate organelle.
For which of the following organelles is this not the case:
1. Mitochondrion
2. Nucleus
3. Chloroplast
4. Peroxisome
5. It is true for all of these organelles

Q3. You are studying a nuclear protein and want to identify its nuclear localization sequence. If you use
molecular genetics to express a construct lacking the first ten amino acids of your protein, it fails to go into the
nucleus. If you express a construct of the ten amino acid sequence fused to a normally cytoplasmic protein, you find
that the fusion protein is cytosolic. Which of the following statements would be true about the ten amino acid
sequence:
1. It is necessary and sufficient to target a protein to the nucleus
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2. It is not necessary nor sufficient to target a protein to the nucleus
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3. It is not necessary but is sufficient to target a protein to the nucleus
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4. It is necessary but not sufficient to target a protein to the nucleus
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5. None of the above

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Q4. Which of the following molecules require a receptor to pass through a nuclear pore:
1.
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ATP
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2. GTP
3. A 20 kilodalton protein
4. A 100 kilodalton protein
5. Both 3 and 4
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Q5. You are studying nuclear import. You mutate the only nuclear import receptor in a cell so that it cannot bind
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Ran-‐GTP but can still bind a cargo protein. Which of the following statements would correctly describe what would
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happen:
1. The import receptor would be found both in the cytosol and nucleus with bound cargo
2. The import receptor would accumulate in the cytosol with bound cargo
3. The import receptor would accumulate in the nucleus with bound cargo
4. The import receptor would accumulate in the cytosol without bound cargo
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5. The import receptor would accumulate in the nucleus without bound cargo
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Q6. Which of the following statements is wrong regarding import of proteins into mitochondria:
1. The precursor protein is first translated in the cytosol
2. The protein is folded after it is transported
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3. The signal sequence binds a receptor in the outer membrane

4. Translocation across both membranes is coupled
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5. None of the above statements are wrong

Q7. What fraction of the different proteins synthesized in a cell are made on the endoplasmic reticulum?
1. About 1%
2. About 10%
3. About 30%
4. About 50%
5. About 80%

The exam continues on the next page

https://www.coursehero.com/file/12081236/Prelim-2-SP14/
The diagram at right shows the membrane of the endoplasmic reticulum and relates to Questions 8-‐10.

Q8. Which of the indicated components is involved in slowing,
or stopping, translation
1. A
2. B
3. C
4. D

Q9. Which of the components is necessary to relieve the
slowed, or stopped, translation?
1. A
2. B
3. C
4. D

Q10. Which of the components is rapidly degraded?
1. A
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2. B
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3. C
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4. D
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Q11 In the diagram of the sequence of a membrane protein shown, if the signal sequence is removed after the
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protein is synthesized, how many times will the polypeptide chain cross the membrane
1.
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Two times
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2. Three times
3. Four times
4. Five times
5. Six Times
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Here is a list of some of the organelles/compartments found in cells; this relates to questions 12-‐17.
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A. cis Golgi
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B. Early endosome
C. Lysosome
D. Endoplasmic reticulum
E. trans-‐Golgi network

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Q12. In which organelle/compartment does the first step of N-‐linked glycosylation occur?
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1. A
2. B
3. C
4. D
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5. E

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Q13. In which organelle/compartment are proteins destined for regulated secretion sorted from those destined
for constitutive secretion:
1. A
2. B
3. C
4. D
5. E


Q14. In which organelle/compartment are mis-‐folded secretory proteins retained and then subject to
degradation:
1. A
2. B
3. C
4. D
5. E

Q15. From which organelle/compartment do COPI vesicles transport material from:
1. A
2. B
3. C
4. D
5. E

Q16. From which organelle/compartment do COPII vesicles transport material from:
1. A
2. B
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3. C
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4. D
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5. E
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Q17. From which organelle/compartment do clathrin vesicles transport material from:
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1. A
2. B
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3. C
4. D
5. E

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Q18. If the t-‐SNARE present on the plasma membrane was removed, which of the following things would start to
happen:
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1. Lysosomal enzymes would be secreted

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2. Secretory proteins would build up in the endoplasmic reticulum

3. Secretory proteins would build up in post-‐Golgi secretory vesicles
4. The LDL receptor would continue take up LDL normally
5. Secretory proteins would no longer be subject to N-‐linked modification

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Q19. If a transport vesicle is accidentally made without a v-‐snare, which of the following events would not
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happen:
1. Cargo would be packaged into the transport vesicle
2. Adaptins would associate with the vesicle
3. The vesicle would become tethered
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4. The vesicle would fuse with its target membrane

5. The vesicle would have bound Rab-‐GTP
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Q20. If dynein is absebnt, which of the following things will occur:
1. The Golgi will disperse
2. The endoplasmic reticulum will collapse
3. Transport from the endoplasmic reticulum to the Golgi will be affected
4. Both 1 and 2
5. Both 1 and 3


The scientists Mike Brown and Joe Goldstein established the receptor-‐
mediated uptake of LDL (Low Density Lipoprotein) by analyzing cells
from patients with hypercholesterolemia. The diagram shows normal
LDL receptor (A), and two mutant receptors (B and C) from such
patients, with the location of the mutation indicated by a star.This
relates to questions 21 and 22.

Q21. Which of these receptors cannot bind LDL:
1. A
2. B
3. C
4. B and C
5. All can bind LDL

Q22. If you treat cells to neutralize the pH of the early endosome, which of these receptors will get trapped in an
early endosome with bound LDL
1. A
2. B
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3. C
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4. A and B
B and C
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5.
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Q23. In which of the following organelles do proteins from the secretory pathway first meet up with material
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from the endocytic pathway
1.
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The Golgi apparatus
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2. The endoplasmic reticulum
3. The early endosome
4. The lysosome
5. They never meet up
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Q24. The pathway for delivery of lysosomal enzymes to lysosomes was elucidated by studying cells from patients
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in which lysosomal enzymes did not get to lysosomes. One of these diseases is called I-‐cell disease -‐ the lysosomal
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enzymes fail to be appropriately modified in the Golgi for recognition by the lysosomal enzyme receptor. In cells from
a patient with I-‐cell disease, which of the following statements is correct:
1. The lysosomal enzyme receptor would be degraded
2. The lysosomal enzymes would be degraded in the trans-‐Golgi Network
3. The lysosomal enzyme receptor would be stuck in the trans-‐Golgi Network
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4. The lysosomal enzyme receptor would be stuck in the early endosome
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5. The lysosomal enzyme receptor would cycle normally between the trans-‐Golgi Network and the
early endosome

Q25. Which phase of the cell cycle is most variable in duration between different cells in an animal:
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1. M
2. G1
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3. S
4. G2

Q26. During which phase of the cell cycle is M-‐cyclin synthesized
1. M
2. G1
3. S
4. G2


The diagram at right relates to questions 27-‐31 and shows inactive M-‐Cdk with two
sites phosphorylated

Q27. What is the component labeled B:
1. CDK
2. M-‐Cyclin
3. APC
4. The kinetochore
5. Kinesin

Q28. Component B is necessary for M-‐Cdk activity because:
1. Without component B, component A cannot bind ATP
2. It moves an inhibitory loop in the structure of component A
3. Component A is degraded without component B

Q29. How is M-‐Cdk activated:
1. By additional phosphorylation
2. By removal of inhibitory phosphate
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3. By removal of activating phosphate
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Q30. Which of the following is activated by a positive feedback loop to fully activate M-‐Cdk:
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1. Wee1 kinase
2. Cdk-‐activating kinase (CAK)
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3. Cdc25 phosphatase.

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Q31. How is active M-‐Cdk turned off when the checkpoint in mitosis is satisfied:
1. By phosphorylation of the inhibitory site
2. By removal of the activating phosphate
3. By degradation of component A
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4. By degradation of component B

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Q32. p53 is a human protein called the 'guardian of the genome'. Which statements about p53 is false:
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1. It is activated by DNA damage

2. It is stably present in cells with no DNA damage
3. It becomes phosphorylated upon DNA damage
4. p53 is a transcription factor that regulates expression of a gene encoding a Cdk inhibitor protein

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Q33. At which checkpoint do cells arrest if DNA synthesis is not complete:
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1. Checkpoint in mitosis

2. G2 checkpoint
3. G1 checkpoint
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Using this list, answer questions 34-‐36 A. Telophase
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B. Metaphase
C. Prometaphase
D. Anaphase-‐Metaphase transition
E. G1 phase
F. G2 phase
G. S phase
H. Prophase
Q34. When do cohesins first function:
1. B
2. C
3. D
4. G
5. H


Q35. When do cohesins get degraded:

1. A
2. C
3. D
4. F
5. S

Q36. The kinetochore is assembled on chromosomes during:
1. B
2. C
3. D
4. F
5. H

Q37. Microtubule disassembly at kinetochores during anaphase is involved in
1. Sliding of inter-‐polar microtubules
2. Movement of chromosomes to poles
3. Activation of the checkpoint in mitosis
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4. Kinesin 5-‐dependent movement
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5. Movement of spindle poles apart
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Q38. During anaphase B, dynein is involved in:
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1. Sliding of inter-‐polar microtubules
2.
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Movement of chromosomes to poles
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3. Activation of the checkpoint in mitosis
4. Kinesin 5-‐dependent movement
5. Movement of spindle poles apart

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Q39. Which type of signaling uses membrane-‐bound signals?

1. Paracrine
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2. Neuronal
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3. Contact-‐dependent
4. Endocrine
5. More than one of the above

Q40. An investigator is studying the effect of hormones on cells and sets up the following experiment: First, he takes
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blood from rats that have been stressed by extreme exercise. Then he uses the blood to treat 3 different types
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of cells which he is growing on independent

dishes. The following day, he observes cells
under the microscope and notices that cells A
have proliferated dramatically, while cells B
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have changed their morphology. The cells in

plate C secrete proteins that turn the media
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red. The investigator explores the following

hypotheses to explain the results from the
experiment. Based on the information
provided and your knowledge on cell
signaling, indicate which one provides a valid
explanation:

1. All three cell types contained the same receptor for a single hormone in the blood of the rat.
2. The three cell types responded to a unique hormone, but they had different receptors.
3. The effects were triggered by three different hormones, each being bound by a different receptor in each
cell type.
4. All of the above.


Indicate whether the following statements about cell signaling are TRUE OR FALSE.
Q41. Steroid hormone receptors elicit changes in cell behavior faster than those elicited by channel-‐coupled
receptors. (1=TRUE; 2=FALSE)
Q42. Second messengers are small molecules that are produced in large quantities inside the cell upon reception of a
signal. (1=TRUE; 2=FALSE)
Q43. In order to stay alive, cells need to receive signals constantly. (1=TRUE; 2=FALSE)
Q44. Drugs can have secondary effects when they target components of a signal transduction pathway that control
multiple effectors within a cell. (1=TRUE; 2=FALSE)
Q45. Fill in the space in the following statement using one of the options below: “When two signals share a common
component of their intracellular signaling pathway, this common component is said to ___________ the two
signals to elaborate a single response.”
1. Relay.
2. Transduce.
3. Integrate.
4. Distribute.
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5. Amplify.
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Q46. Indicate which one of the statements below about meiosis is TRUE:
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1. During the first meiotic cell division, the spindle separates sister chromatids.
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2. During the second meiotic cell division, homologous chromosomes undergo recombination.
3. The first meiotic cell division is very similar to mitosis, but the resulting cells are haploid because the DNA
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does not replicate previously.
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4. More than one statement is true.
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5. None of the statements is correct.

Q47. Which one of the following adult tissues originates from the ectoderm germ layer:
1. Gut, liver and lungs.
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2. Skeleton and muscle.

3. Heart and blood.
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4. Nervous system.
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5. None of the above.

THE FOLLOWING TEXT AND DRAWING REFER TO QUESTIONS Q48 TO Q51 (see below):
A graduate student is performing a transplantation experiment in bird embryos. To be able to trace the origin of cells
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after the transplantation, he uses two different species of birds which have a different pigmentation. He uses as
donor tissue a region of the hindlimb (leg) of pink-‐pigmented bird embryos. He transplants this piece to the anterior
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region of the forelimb (wing) primordia of a blue-‐pigmented bird. The transplanted tissue heals and the graduate
student finds that the treated wing in the resulting blue-‐pigmented bird develops normally and contains some pink-‐
pigmented areas in the anterior part. Indicate whether the following statements are TRUE or FALSE:
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Q48. The fate of the transplanted tissue was to become wing. (1=TRUE; 2=FALSE)
Q49. The transplanted tissue was already determined to become wing before the transplantation (1=TRUE; 2=FALSE)
Q50. The transplanted tissue was specified as wing tissue after the transplantation (1=TRUE; 2=FALSE)
Q51. In this experiment some cells could induce others to change their fate (1=TRUE; 2=FALSE)
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BioMG1350 Prelim.

2- K Spring 2014 - ANSWER KEY

DO NOT OPEN THE EXAM UNTIL

• Put ALL belongings under your seat.

transfer them to the Scantron.

• Answer the easy questions first, and go back to the harder

https://www.coursehero.com/file/12081236/Prelim-2-SP14/ The exam starts on the next page

Q1. The version of this test is:

3. The signal sequence binds a receptor in the outer membrane

5. None of the above statements are wrong

The exam continues on the next page

The exam continues on the next page

1. Lysosomal enzymes would be secreted

2. Secretory proteins would build up in the endoplasmic reticulum

4. The vesicle would fuse with its target membrane

The exam continues on the next page

The exam continues on the next page

4. By degradation of component B

1. It is activated by DNA damage

1. Checkpoint in mitosis

The exam continues on the next page

Q35. When do cohesins get degraded:

Q39. Which type of signaling uses membrane-­‐bound signals?

of cells which he is growing on independent

have changed their morphology. The cells in

red. The investigator explores the following

The exam continues on the next page

2. Skeleton and muscle.

5. None of the above.

This is the last page of the exam

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Q39. Which type of signaling uses membrane-‐bound signals?