MICRO-NANO SYSTEM

ENGINEERING LABORATORY

LAB MANUAL

STEREO MICROSCOPIC
 INTRODUCTION
 For three dimensional perspective (3D).
 For viewing relatively large and solid surfaces or specimens.
 For detailed work such as microsurgery, watch making and circuit
board inspection and manufacturing.

APPARATUS
 Stereo Microscopic
 Specimen
 PC, Software
 SIDE VIEW OF STEREO MICROSCOPIC
 Right View of Stereo Microscopic  Left View of Stereo Microscopic
 NOMENCLATURE DESCRIPTIONS
Zoom adjustment knob Left/right single-shaft horizontal knobs
(with high/low zoom magnification
stopper)
Camera Port Install and uninstall the camera
Light path selector lever • Binocular Observation – Push the lever
• Camera Observation – Pull the lever

Power Supply To switch ON/OFF light.

Focus adjustment knob The magnification can be limited in the

desired range by setting each stopper on
the microscope body (also used as the
magnification indices) and the stopper ring
on each zoom adjustment knob. The right
zoom adjustment knob is used to limit the
higher end of the magnification, and the
left knob is used to limit the lower end.
X and Y axis knob To move the specimen to a desired
position, rotate the X-axis knob and Y-axis
knob.
 NOMENCLATURE DESCRIPTIONS
Eyepiece/ Observation Tube To observed the objective.

Microscope Body · SZ61

· SZ51
· SZ61-60
· SZ51-60
· SZ61TR
Stage Plate When reflected light illumination is
used, the stage plate can be placed
either the white or black surface
facing up depending on which side
makes the specimen easier to view.
• ESD performance, use the black
surface
• Transmitted light illumination, use
transparent glass stage plate.
X and Y axis knob To move the specimen to a desired
position, rotate the X-axis knob and
Y-axis knob.
 Observation Procedure

START

Put the specimen on

the stage center plate

Turn ON power

Adjust the interpupillary

distance.
• Observed the specimen from the
Adjust the eyepiece diopter.
eyepieces 2
• Rotate the zoom adjustment knob 3 to
Observed the
the target magnification and rotate the
specimen from the
focus adjustment knob 4 to bring the
eyepieces
specimen into accurate
• (Please refer to the next page for
GOOD figure)
NO

YES
END
 INSTRUCTION FIGURE
Adjust the interpupillary distance 1

Adjust the eyepiece diopter.

• Observed the specimen from the

eyepieces 2
• Rotate the zoom adjustment knob
t 3 to the target magnification
and rotate the focus adjustment
knob 4 to bring the specimen
into accurate
 Mounting the TV Camera

1. Using the Allen wrench, loosen the C-mount clamping

screw 1 and remove the C-mount seat 2.
2. Screw the C-mount seat 2 into the TV camera 3.
3. Place the C-mount seat in the original position and tighten
the clamping screw 1.
4. Connect the cables and monitor to the TV camera.
 Procedure To Capture Image Using Software
analySIS getIT- Step By Step

1.Double click on icon analySIS getIT

2.Click on icon (a) and the DigiAcquis TWAIN window would appear.
d a c

3. Click on icon (b) to capture the image.

4. Click on icon (c) to set the correct scale bar / magnification

for the image.
5. To burn the scale on the image, click on “Image”, then “Scale Bar” then
“Burn into Image”.

6. Click on icon (d) to save the image to the desired folder.

Note: It is compulsory to save the file as type Tagged Image Format “tif” for
the images which require measurement in the future.
measureIT – Step By Step
1. Close the analySIS getIT application.
2. Double click on icon measureIT.
3. Click on icon (e) to apply the scale bar. The scale bar will be accurate if
the image was saved as “tif” file.
4. Click on icon (f) to do measurement.
5. Click on icon (g) for some annotation on the image.

e f g
 CALIBRATION
BY DIFFERENCE ZOOMING
0.6X 1.2X

1.5X 1.0X
2.5X 3.5X

2.0X 3.0X
4.5X 4.0X
Prepared by: Approved by:

……………………………………. …………………………………….
Nurul Nadiah Binti Bakri Dr. Mohd Ridzuan Ahmad
Research Assistance (RA) Dept. of Control and Mechatronics Engineering,
Dept. Control and Mechatronics Engineering Faculty of Electrical Engineering,
Faculty of Electrical Engineering UTM, Skudai.
UTM, Skudai.