PRP THERAPY KIT (PRF Preparation)
IN CHRONIC WOUND ULCERS AND DIABETIC FOOT ULCERS
Platelet rich plasma (PRP) application is a successful
catalyst in the healing process for wide varieties of
conditions. PRP therapy began gaining popularity in the
mid 1990s. Methods of harvesting and using platelet rich
plasma are becoming widely practiced in the fields of
sports medicine, orthopedics, traumatology, general
surgery, dentistry, dermatology and cosmetology and
aesthetic medicine.
Main function is hemostasis by attaching to any breach
in vessel where platelets aggregate, activate and release
granules. Alpha granules contain clotting mediators such
as factor V, factor VIII, fibrinogen, fibronectin, platelet-
derived growth factors, and chemotactic agents. Delta
granules, or dense bodies, contain ADP, calcium,
serotonin, which are platelet-activating mediators.
Activated platelets release granules by exocytosis which
contain more than sixty different biologically active
substances that are involved in processes of tissue
regeneration, angiogenesis, epithelialization,
proliferation and differentiation of osteoblasts, and in
synthesis of collagen and extracellular matrix of
connective tissue.
Use of PRP in Non-healing skin Ulcers :
Any wound goes through stages of acute inflammation,
increased mitotic activity and finally orderly repair.
Chronic wounds can be those wounds failing to proceed
through an orderly and timely process produce anatomic
and functional integrity. Practically, a chronic wound is
the one which has failed to heal within 3 months.
Repeated trauma, foreign bodies, pressure necrosis,
infection, ischemia and tissue hypoxia are few of the
reasons of non healing. Growth factors are crucial for
timely wound healing. Platelets can fill the gap by
supplying growth factors. It is desirable to supply
growth factors over extended time and this can be
achieved by delivering platelets in fibin matrix.
Fibrin membranes are very useful for treating chronic
diabetic non healing ulcers, and pressure sores. Use of
fibrin membranes in such cases can prepare the wound
for skin grafting earlier, reducing the time for total
healing. This results in improved quality of life and
lower cost of care over other conventional therapies.
PRP Product to be used :
Concentrated PRF preparations ( typically 7-10 times
concentration of platelets in fibrin clot) are used in these
conditions. Quantity required varies depending on area
of wound and PRF membranes can be prepared with the
size of 60 mm discs. Depending on the area required
number discs can be prepared.
Material provided:
1. PRP-50 sterile anticoagulant Ampoule 5 ml.
2. PRP activator 1 ml ampoule
3. PRP Test tubes 15 ml 3 nos Sterile
4. PRP Transfer pipettes 3 ml 1 pair Sterile
5. Sterile disposable 60 mm petri dishes
(All material sterile for 3 years from manufacture)
Other Material required:
1. Centrifuge 15 ml and/or 50 ml rotor with timer
and RPM Meter.
2. Incubator with digital control
3. Test Tubes stand for 10 ml and 50 ml tubes
Procedure :
1. Take 10 ml, 15 ml or 50 ml tube as per the need
2. Add 1.5 ml or 5 ml anticoaguland and collect
13.5 ml blood by using scalp vein set 18 No.
directly to the anticoagulant added tubes.
Depending on need additional number of tubes
can be set-up. Discard remaining anticoagulant.
3. Cap the tubes tightly and Mix well by inversion
several times.
4. Centrifuge for 15 min at 1500 RPM
 5. Plasma rich in platelets will be separated. At 10. Apply appropriate dressing and leave the
least 50% volume should be plasma on top and dressing for 5-7 days.
there should not be any buffy coat on the RBC
layer. If plasma is less, centrifugation needs to
be increased and if buffy coat forms,
centrifugation needs to be reduced. What to expect :
6. Transfer plasma to sterile petri dishes using
The PRF membrane releases growth factors over next
sterile pipette. Petri dishes should be kept
one week and rapid growth of healthy granulation tissue
horizontal on vibration free surface.
is expected in 1-2 weeks. The Membrane application can
7. Add 0.5 ml activator for each 10 ml plasma in
be repeated 1-2 times as required depending on the
that proportion and mix gently.
healing and suitability of grafting.
8. Cover the petri dish with its cover in incubator at
37C and allow to clot for 10-15 min.
9. Very thin clot membrane will form which then is
applied to wound directly from the plate.

Please contact Dr. S K Hayatnagarkar, Cryobank Fertility Research Centre, Kamgar Chowk, N-
2, CIDCO Aurangabad Cell & Whatsapp: 9422725000, 9890900585