EFEK DEKOKTA PELEPAH DAUN NIPAH (nypa Fructicans Wrumb) Terhadap KADAR SOD dan MDA

JANTUNG TIKUS MODEL DMT-2

Yowanda Avif Muftidiyanto, Helmin Elyani, Arif Yahya

Mahasiswa Fakultas Kedokteran Universitas Islam Malang
E-mail : avifdrsp@gmail.com

ABSTRAK

Pendahuluan : Diabetes mellitus tipe 2 merupakan salah satu faktor resiko terjadinya penyakit kardiovaskuler. Hal ini
berhubungan pemekatan gula darah yang meningkatkan stress oksidatif pada jantung yang di tandai oleh perubahan
kadar SOD dan MDA pada jantung. Kandungan flavonoid, tannin, saponin dan fenol pada pelepah daun nipah
berpotensi sebagai antidiabetes dan antioksidan. Tujuan penlitian ini adalah melihat efek pelepah dau nipah terhadap
kadar SOD dan MDA jantung.
Metode: Penelitian in vivo ini dengan desain control group post test only menggunakan tikus wistar jantan dengan
diinduksi HFD dan STZ single dose 35 mg/kgBB. Pemberian dekokta pelepah daun Nipah dosis 200, 400 dan 800 mg
diberikan selama 30 hari. Pengukuran kadar SOD dan MDA jantung menggunakan uji colorimetric. Data dianalisa
selanjutnya diuji dengan one way ANOVA dan dilanjutkan dengan LSD (p≤0.05).
Hasil: induksi DMT-2 mampu menurunkan SOD dan meningkatkan MDA masing-masing 26% dan 21% di banding
kontrol normal. Pemberian dekokta pelepah daun nipah dosis 200 dan 400 mampu meningkatkan kadar SOD masing-
masing 26% dan 16% dan menurunkan MDA jantung sebesar 24% dan 17% sedangkan dosis 800 dapat meningkatkan
kadar SOD dan menurunkan MDA jantung sebesar 31% dan 4%.
Kesimpulan : dekokta pelepah daun nipah dosis 400 mampu menurunkan kadar MDA dan meningkatkan kadar SOD
jantung.

Kata Kunci: : Dekokta Pelepah Daun Nipah, Diabetes Melitus Tipe-2 (DMT-2), kadar SOD-MDA Jantung.

NIPAH MIDRIB LEAF (nypa Fructicans Wrumb) EFFECT TO THE LEVEL OF HEART SOD AND MDA
ON DMT-2 RATS MODEL
Yowanda Avif Muftidiyanto, Helmin Elyani, Arif Yahya
Student of Medical Faculty of Universitas Islam Malang
E-mail : avifdrsp@gmail.com

ABSTRACT

Background: Type 2 diabetes mellitus is one of the risk factor for cardiovascular disease. This mechanism is caused by
increased blood glucose viscosity that can also increase oxidative stress in the heart marked by changed MDA and SOD
level on the heart. Flavonoid, tannin, saponin and fenol from nipah midrib leaf can be potentially used as antidiabetic
and antioxidant. This research aims to observe the effect of nipah midrib leaf to the heart SOD and MDA level.
Methods: This in vivo research was done using control group post test only design on male wistar rats induced with 35
mg/kgbw of HFD single dose and STZ. Supplementation of decocta nipah midrib leaf with the dose of 200, 400 and
800 mg was done for 30 days. Heart SOD and MDA level was measured with colorimetric test. The data was then
analized with one way ANOVA continued with LSD (p<0.05)
Result: DMT-2 induction can reduce SOD and increase MDA level by 26% and 21% compared with control group.
Supplementation with decocta nipah midrib leaf at the dose of 200 mg and 400 mg can increase SOD level by 26% and
16% respectively and also able to reduce MDA level by 24% and 17%. Meanwhile, supplementation with the dose of
800 mg can increase SOD and reduce MDA level to 31% and 4%.
Conclussion: Decocta nipah midrib leaf at the dose of 400 mg can reduce heart MDA and increase heart SOD level.

Keywords: Decocta midrib leaf, Type-2 Diabetes Mellitus (DMT-2), Heart SOD and MDA Level