Professional Documents
Culture Documents
CHM 112
11/21/2015 dcapodaca_CHM112_MAPUA 2
• Ethylene diamine – bidentate
• Adenosine triphosphate – tetradentate
• Aminocarboxylic acids – synthetic chelating agents
11/21/2015 dcapodaca_CHM112_MAPUA 3
EDTA Titration Curves
• Mn+ + EDTA = MYn-4
• Graph of pM (= - log [M]) versus the volume of
added EDTA
• Region 1: Before the Equivalence point
– There is excess Mn+ left in solution after the EDTA
has been consume. The concentration of free
metal ion is equal to the concentration of excess,
unreacted Mn+
11/21/2015 dcapodaca_CHM112_MAPUA 4
• Region 2: At the Equivalence point
– There is exactly as much EDTA as metal in the
solution
– Some free Mn+ is generated by the slight
dissociation of MYn-4
– MYn-4 = Mn+ + EDTA
– EDTA – total concentration of free EDTA in all of its
forms
– At the equivalence point, [Mn+ ] = [EDTA]
11/21/2015 dcapodaca_CHM112_MAPUA 5
• Region 3: After the Equivalence point
– Excess EDTA
– All the metal ion is in the form MYn-4
– The concentration of free EDTA can be equated to
the concentration of excess EDTA added after the
equivalence point
11/21/2015 dcapodaca_CHM112_MAPUA 6
Titration Calculations
• Calculate the shape of the titration curve for
the reaction of 50.0 mL of 0.0500 M Mg2+
(buffered to pH 10.00) with 0.0500 M EDTA
• K’f = 2.2 x 108
• Equivalence volume is 50.0 mL
• Region 1: before the equivalence point
– Determine [Mg2+] = fraction remaining x original
concentration of metal x dilution factor
11/21/2015 dcapodaca_CHM112_MAPUA 7
• Region 2: at the equivalence point:
– [MgY2-] = original concentration of metal x dilution
factor
– K’f = 2.2 x 108 = [MgY2-] / [Mg2+][EDTA]
11/21/2015 dcapodaca_CHM112_MAPUA 8
Metal ion indicators
• A compound whose color changes when it
binds to a metal ion
11/21/2015 dcapodaca_CHM112_MAPUA 9
• MgIn (red) + EDTA = MgEDTA + In (blue)
11/21/2015 dcapodaca_CHM112_MAPUA 10
EDTA Titration Techniques
• Direct titration – analyte is titrated with
standard EDTA. The analyte is buffered to an
appropriate pH at which the conditional
formation constant for the metal-EDTA
complex is large and the color of the free
indicator is distinctly different from that of the
metal-indicator complex.
11/21/2015 dcapodaca_CHM112_MAPUA 11
• Auxiliary complexing agent prevents the metal
ion from precipitating in the absence of EDTA.
11/21/2015 dcapodaca_CHM112_MAPUA 12
• Back titration – a known excess of EDTA is
added to the analyte. The excess EDTA is then
titrated with a standard solution of a second
metal ion.
11/21/2015 dcapodaca_CHM112_MAPUA 13
• Displacement titration – the analyte is treated
with excess Mg(EDTA)2- to displace Mg2+,
which is later titrated with standard EDTA.
• Hg2+ determination
11/21/2015 dcapodaca_CHM112_MAPUA 14
• Indirect titration – anions that precipitate with
certain metal ions can be analyzed with EDTA
11/21/2015 dcapodaca_CHM112_MAPUA 15
• An anion can be precipitated with excess
metal ion. The precipitate is filtered and
washed, and the excess metal ion in the
filtrate is titrated with EDTA.
11/21/2015 dcapodaca_CHM112_MAPUA 16
• Masking agent – a reagent that protects some
component of the analyte from reaction with
EDTA.
11/21/2015 dcapodaca_CHM112_MAPUA 18