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ANNA UNIVERSITY, CHENNAI

QUESTION BANK

BT2251 BASIC INDUSTRIAL BIOTECHNOLOGY

B.TECH BIOTECHNOLOGY

UNIT-1
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INTRODUCTION TO INDUSTRIAL BIOPROCESS

PART-A
1. What is fermentation?
2. Comment on continuous culture. May-2007
3. Comment on batch culture.
4. Define batch fermentation process.
5. Define submerged culture process.
6. Define surface culture process.
7. Comment on anaerobic fermentation.
8. Comment on aerobic fermentation process.
9. Short notes on different type of sterilization methods. May-2007
10. Define the role of genetic engineering in bioprocess. May-2007
11. Comment on pH and biomass reduction in fermentation technology. May-2007
12. Name any two significant milestones in the history of biotechnology. Dec-2003

PART-B
1. Write a brief note on different fermentation products? May-2007
2. Explain the principle of a fermenter with a clear diagram. May-2007
3. Overview of downstream processing; centrifugation, filtration and ultra filtration.
May-2007
4. Comment on interactions between fermentation and recovery. May-2007
5. What are the different types of reactors used in the biotech industry? May-2006
6. Give a pictorial representation of production of alcohol.
7. Give a brief survey of organisms and products related to modern biotechnology.
8. Write a historical overview of industrial fermentation process.
9. Explain with block diagrams the overall industrial bioprocess.

UNIT-2
PRODUCTION OF PRIMARY METABOLITES
PART-A
1. What are primary and secondary metabolites? Dec-2005
2. What is downstream processing? Dec-2007
3. Applications of aspartic acid and acetic acid.
4. Differentiate between liquors and spirits. May-2006
5. With the help of a flowchart show the production of citric acid by precipitation
method.
6. Give two methods by which L-glutamate production in C.glutamicum is increased.
7. Application of butanol.
8. Give a outline of processes for production of vinegar.
9. What is yellow oil?
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10. What are the by products produced during ethanol production?

11. Give a scheme of a plant for the production of absolute alcohol. May-2006

PART-B
1. Write a note on the commercial use of amino acids. May-2006
2. Discuss strategies to improve production of amino acids. May-2006
3. Give a scheme for the material flow in L-glutamate production plant and discuss the
production process along with a scheme of main reactions in L-glutamate formation
from glucose. May-2006
4. How is citric acid produced? Give a flowchart for the manufacture of citric acid. May-
2006
5. What are the applications of citric acid? May-2006
6. Write in detail the method of production of aspartic acid. Dec-2005
7. Describe in detail about ethanol production with a neat diagram and explain about the
purification process and its applications in various fields. Dec-2005
8. How is lactic acid produced? Give a flowchart for the manufacture of lactic acid.
9. Describe in detail about acetic acid production with a neat diagram.

UNIT-3
PRODUCTION OF SECONDARY METABOLITES
PART A
1. What are wild and type strains? Dec-2005
2. What are amino glycosides? Explain its application.
3. Define mutasynthesis. May-2006
4. Give an example of an amino glycoside antibiotic, its application and a producing
strain.
5. What are the precursors used in the production of penicillin G and penicillin V?
6. What are macrolides? Give an example.
7. What are steroids?
8. What are beta-lactams?
9. What are secondary metabolites?
10. Give an outline for the production of cephalosporin.
11. Application of vitamins in industries.

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12. Give the structure of erythromycin.

13. What are the types of penicillin and its application?
14. Write the importance of macrolides. Dec-2005

PART-B
1. Explain the steps involved in the production of vitamins and steroids with one
example each. May-2007
2. Explain the step involved in the production and purification of penicillin from the
microbial broth. May-2007
3. What is the process for the production of β lactam antibiotics and the recovery of the
product? May-2007
4. How can the production of secondary metabolites be enhanced?
5. Write in detail about the production of amino glycosides.

UNIT-4
PRODUCTION OF ENZYMES AND OTHER BIOPRODUCTS
PART-A
1. Write the application of proteases. Dec-2005
2. What are the biopesticides? Give an example. Dec-2005
3. Name two methods by which enzyme productivity can be increased many-fold. May-
2006
4. What is nisin and its application? May-2006
5. What are biofertilizers? Nov-2003
6. Name and discuss any one of the advantages and disadvantages in using whey as an
industrial raw material. Dec-2003

PART-B
1. Write a note on PHA, PHB and biopol and its importance in medicine and commerce.
2. How is PHB metabolism regulated? May-2006
3. Discuss production of PHA by plants microbial sources. May-2006
4. Describe the 2-stage bioprocess for the production of PHA. May-2006
5. Give a flowchart for the production of cheese. May-2006
6. Write a detailed note on the manufacture of cheese. May-2006
7. How can you recover and isolate α amylase from Bacillus amyloliquefaciens?

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8. Describe giving details any two industrial products (proteases and lipases) using
enzymes. Nov-2003
9. Explain industrial production of cellulases and their applications. May-2007
10. Explain in brief about bio pesticides. May-2007
11. What are bio fertilisers?
12. Describe in detail about bio preservatives and biopolymers with relevant examples.
13. Discuss a strategy for the production of bio pesticides from Bacillus sphaericus. May-
2006
14. What is single cell protein? What are the types of micro organisms used for its
production and write its nutritional value. Dec-2005

UNIT-5
PRODUCTION OF MODERN BIOTECHOLOGY PRODUCTS
PART-A
1. Role of air-lift reactor in plant cell culture. May-2007
2. Explain in brief “animal cell culture”. May-2007
3. Differentiate between “callus culture” and “suspension cultures”. May-2006
4. Why mouse is a useful experimental animal model? Nov-2003
5. What are recombinant proteins? Nov-2005
6. What are MAbs?
7. Application of MAbs.
8. Application of recombinant proteins for diagnostic purpose.
9. What are vaccines?
10. What are the products of plant cell culture?
11. What are the products of animal cell culture?
12. Give examples for vaccines.

PART-B
1. Discuss about the production of recombinant proteins with any commercially
important protein as examples. May-2007
2. Discuss in detail about the production and various uses of monoclonal antibodies.
May-2007
3. Outline the process for the cultivation of mammalian cells. May-2006

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4. How mammalian cells are genetically engineered? May-2006

5. How can perfusion cultures be used in biomass production of mammalian cells?
6. What are recombinant proteins? How are they produced?
7. What are the products of plant and animal cell culture?
8. Explain the therapeutic and diagnostic application of recombinant proteins.

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